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Inheritance Study of Male Sterile Transformants Containing Pollen-specific Promoter and Diphtheria Toxin A Gene (수술특이프로모터와 디프테리아 독성 유전자에 의한 웅성불임 형질전환체의 후대 유전분석)

  • Park, Young-Doo;Kim, Hyun-Uk;Park, Beom-Seok;Jin, Yong-Moon
    • Horticultural Science & Technology
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    • v.18 no.3
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    • pp.342-347
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    • 2000
  • The objectives of this study were to investigate the genetic and phenotypic features of male sterile transformants by pollen-specific expression of diphtheria toxin gene and to find out inheritance patterns of transgene to the next generation. When backcrossed (BC) progenies were tested for expression of kanamycin resistance ($Km^R$), 9 lines out of 13 lines, except 4 lines ($BC_{1}5-13,\;BC_{1}5-23,\;BC_{1}5-28,\;BC_{1}5-32$), showed the ratio of $Km^R$ to kanamycin sensitive ($Km^S$), from 1:30 to all $Km^S$. As a result, they were much lower than Mendelian segregation of a dominant gene. To determine whether male sterility is a heritable and stable trait, 5 male sterile plants ($BC_{1}5-13,\;BC_{1}5-14,\;BC_{1}5-23,\;BC_{1}5-32,\;BC_{1}5-33$ lines) which had different transgene copy numbers were backcrossed as female parents with pollens from wild type. To confirm the existence of the DTx-A gene in the genome of the progenies, PCR was conducted using specific primers of the DTx-A coding region. A PCR band of 428 bp was obtained from each generation, which is the predicted size of the DTx-A gene fragment. Trangenes were inherited to the next $BC_4T_0$ progenies and showed male sterility, however, based on the copy numbers of DTx-A gene male sterile plants did not show predicted ratio. When male sterile plants were backcrossed with fertile plants, fruit capsule sizes and seed settings were relatively reduced from those of selfing wild type plants. The fruit sizes and seed settings were reduced in proportion to the increase in the copy number of DTx-A gene.

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Study on Air Logistics Service Provider's Performance Under Post Covid19 Situation: Focusing on Trust and Reciprocity (포스트 코로나 시대의 항공물류 서비스기업의 경영성과에 대한 연구: 신뢰 및 호혜 개념을 중심으로)

  • Moon, Myungjoo;Koh, InKon
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.17 no.3
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    • pp.135-145
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    • 2022
  • Due to COVID 19, we are facing unprecedented phenomena. Among various industries, the aviation industry, as it is directly affected by the corona crisis is actively establishing various survival strategies, such as expanding cargo transportation. The market size of air cargo transportation is continuously increasing due to the characteristics that distinguish it from other transportation, and as individualism and selfishness deepen in the aftermath of COVID 19, it can be inferred that the concept of reciprocity within distribution channels will become important in the post-corona era. The specific contents of this study(research question) are as follows. First, as a central member of the air logistics distribution channel, logistics service companies business process and the sub-dimensions of trust of are identified, and how trust is built with transportation companies is investigated. Second, the effect of such trust on the various performances of logistics service companies is analyzed. Third, we examine whether the influences of the sub-dimensions of trust change according to the perceived reciprocity of logistics service companies. In addition, we investigate whether the perceived reciprocity changed before and after the corona situation. In particular, this study theoretically integrates the concepts of trust and commitment, which have been distinguished in many prior studies, to improve the parsimony and practicality of the research model. This study will be able to present useful academic and practical implications by empirically examining how trust is built between members of the air logistics distribution channel and furthermore, how much it affects the performance of logistics service companies, and by identifying the moderating effect of reciprocity.

Facilitated Transport: Basic Concepts and Applications to Gas Separation Membranes (촉진수송: 기본 개념 및 기체분리막 응용)

  • Park, Cheol Hun;Lee, Jae Hun;Park, Min Su;Kim, Jong Hak
    • Membrane Journal
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    • v.27 no.3
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    • pp.205-215
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    • 2017
  • Polymer membranes are cheap and easy in fabrication, and show a high permeability and selectivity, thus play pivotal roles in gas separation as well as water purification. However, polymer membranes typically exhibit the trade-off relation between permeability and selectivity; i.e. when the permeability is high, the selectivity is low and vice versa. Facilitated transport has been considered one of the solutions to address this issue. Over the last decades, facilitated transport concept had played an important role in preparing the membranes and providing ideal and various models for the transport. Understanding the nature of carrier, the mobility of matrix and the physico-chemical properties of polymer composites are crucial for facilitated transport. Depending on the mobility of carrier, facilitated transport membrane is classified into three; mobile carrier membrane, semi-mobile carrier membrane, fixed-site carrier membrane. Also, there are four types of reversible reaction between the carrier and the specific target; proton transfer reaction, nucleophilic addition reaction, p-complexation reaction and electrochemical reaction. The facilitated transport membranes have been applied in the separation of CO2, O2 and olefin (propylene or ethylene). In this review, major challenges surrounding facilitated transport membranes and the strategies to tackle these challenges are given in detail.

Proposition of a Practical Hybrid Model for the Valuation of Technology (기술가치평가를 위한 실용적 하이브리드 모델의 제안)

  • Park, Hyun-Woo;Nah, Do-Baek;Park, Jong-Kyu
    • Management & Information Systems Review
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    • v.28 no.4
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    • pp.27-44
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    • 2009
  • Economic value of a certain technology is of great interest and importance in a wide variety of investment circumstances. These vary from companies considering investing in R&D projects, to venture capitalists funding start-up companies. However, such valuation is extremely difficult in any case, and the cost of failure can be very high. Many techniques have been proposed to assist managers facing this issue, from traditional discounted cash flow analysis to more recent methods based on real options. In the meantime, the discounted cash flow method has limitations in applying the valuation of technology. At the same time, there have been various solutions to overcome theoretical problems of the method. Real options have been thought as a solution. However, there are another problems in using them in real world. This paper reviews the previous studies on the valuation of technology in several aspects, discusses the practicability of the various methods available, and explore the application of a hybrid model, which aims to make these rather aore the ideas more accessible to practicing managers.

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Introductions of Pre-Rake with Frequency Domain Equalizer and Cyclic Prefix Reduction Method in CDMA/TDD Multi-code Transmission (CDMA/TDD 다중코드 전송에서 주파수 도메인 등화기와 결합된 Pre-Rake 와 Cyclic Prefix 최소화 방법)

  • Lee, Jun-Hwan;Jeong, In-Cheol
    • Journal of Satellite, Information and Communications
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    • v.6 no.1
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    • pp.86-96
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    • 2011
  • In this paper we propose a Pre-rake system applied with a frequency domain equalizer in TDD/CDMA multi-code transmission. The Pre-rake system has been well known technique in TDD/CDMA to make a receiver simple. However, it still has residual losses of path diversity and signal to noise ratio (SNR). However, gathering all the residual paths demands an additional hardware such as a rake combiner at the receiver. For the reason Pre/Post-rake system has already been proposed at up/downlink correlated channel conditionunder the assumption of noisier channel. There is a trade-off between the first purpose of Pre-rake that makes hardware simple at the receiver and the performance improvement. From the point the frequency domain equalizer (FDE) can be considered in Pre/Post-rake to supply the receiver with the flexible equalizing methods with rather reduced complexity compared with time domain rake combiner or equalizers. Pre-rake itself increases the number of multipath, which results from the convolution of Pre-rake filter and wireless channel, and FDE must be well matched to Pre/Post-rake, while it considers the relationship of hardware complexity and the performance. In this paper, the Pre-rake/Post-FDE system is introduced at TDD/CDMA multi-code transmission. In addition, the cyclic prefix reduction method in the proposed system is introduced, and the theoretical analysis to the proposed system is given by assuming Gaussian approximation, and finally the numerical simulation results are provided.

Game Analysis for Next Generation User Interface based on Storytelling (차세대 스토리텔링기반 유저 인터페이스를 위한 게임분석)

  • Lee, Dae-Young;Kim, Seon-Ju;Yu, Hui-Beom;Won, Sun-Jin;Sung, Jung-Hwan
    • 한국HCI학회:학술대회논문집
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    • 2008.02b
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    • pp.534-539
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    • 2008
  • Nowadays, UI(User Interface)'s main target area is getting larger from user's convenience oriented interface, to user's satisfaction oriented interface. In this study, we attempted 'Storytelling User Interface' for fulfillment of user's satisfaction. We analyzed UI in games, distinguished and clarified its effectiveness that conveys main contents and special features to its audience. The most important element in storytelling UI is 'Inherent Drama'. This was proved through the game analysis with Blizzard's 'Diablo', Sierra's 'Home world', Eden Games' 'Test Drive Unlimited', EA's 'Black And White', and Sony Entertainment's 'Eye Of Judgment'. And we divided storytelling elements into 3 pieces, Mood Design - that shows story's background, or traits, Natural Control - for user's feeling as a hero in the content, and Flow Directing - using animation, visual design and sound, etc. Via these elements, we can make the best use of background, icon, animation, every structures and flow in games. Finally, we embodied three pieces for putting in practice for effective improving interface.

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Development of OSL Dosimetry Reader (선량 판독용 OSL 측정장치의 개발)

  • Park, Chang-Young;Chung, Ki-Soo;Lee, Jong-Duk;Chang, In-Su;Lee, Jung-Il;Kim, Jang-Lyul
    • Journal of Radiation Protection and Research
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    • v.37 no.1
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    • pp.10-15
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    • 2012
  • Design and performance test results of a newly developed optically stimulated luminescence (OSL) measurement system are presented in this paper. Generally, different types of optical filters are used in OSL reader system to minimize the interference of the stimulation light in the OSL signal. For optically stimulation of $Al_2O_3:C$, we have arrived at an optimal combination of the filters, i.e., GG420 filter for filtering the stimulating light source, and a combined UG11 and BG39 filter at the detecting window (PMT). By using a high luminance blue LED (Luxeon V), sufficient luminous intensity could be obtained for optically stimulation. By using various control boards, the OSL reader device was successfully interfaced with a personal computer. A software was developed to deliver required commands to operate the OSL reader by using the LabView program (National Instruments, Inc.). In order to evaluate the reliability and the reproducibility of newly designed-OSL reader. Performance testing of the OSL reader was carried out for OSL efficiency, OSL decay curve and signal to noise ratio of the standard $Al_2O_3:C$ OSL material. It was found to be comparable with that of commercial Riso reader system.

A study on the new Business Opportunity of E-Commerce (e-비즈니스의 기회창출 방안에 대한 연구)

  • Cho, Jae-Wan;Ko, Chang-Bae
    • Information Systems Review
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    • v.4 no.2
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    • pp.191-208
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    • 2002
  • In the area of new millennium of twenty first century, one of the urgent and critical research issues in commerce area is the regenerating of new business opportunities from the high value added perspectives. With this motivation, in this paper, we create new on-line e-businesses with the speed of lightning their affiliated supply, finance and business communities - which are involved in more and more tightly connected, open trading - we find that we need to deal with hundreds of business collaborative partners, millions of buyers and sellers while we have to face incompatible IT systems. Challenges of new business opportunities linking multi-enterprise data and processes cost effectively, reliably and securely in real time remains an open area in e-business. This challenge we shall describe as the problem of synchronization of multiple enterprise collaborative e-business opportunities (production related), value (finance related), business (operations related) in new business opportunity and infrastructure integrated all together over the off-line and online basis. It brings a new e-commerce opportunities infrastructure into this profitable challenge: by extracting and tracking new business information, new trends in the events of e-business processes. The transformation of the traditional commerce into this type of electronic based commerce can be interpreted as new Cultural Revolution. The revolution will be a new paradigm crossing over the geographical, and organizational zone, restructuring enterprise business process infrastructure.

Development of Cut-in Lane Changing Model Based on Observed Driver's Behavior in Uninterrupted Traffic Flow (연속교통류에서의 끼어들기 행태 분석 및 모형 개발)

  • Kim, Kyung-Hee;Chang, Myung-Soon;Kim, Jin-Tae;Kim, Eung-Cheol
    • Journal of Korean Society of Transportation
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    • v.22 no.6
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    • pp.145-157
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    • 2004
  • Microscopic freeway simulation models project the uncertain values of performance measures for subject traffic conditions by explaining drivers' driving behavior with lane changing and car-following models. However, the existing lane changing models are limited to gap acceptance oriented passive behavior of drivers and not able to capture more-or-less aggressive driving behavior(e.g. cut-in lane changing) ordinarily obseved in field. This paper suggests the definition of cut-inlane changing and presents its characteristics based on the findings from two different freeway on- and off-ramp sections. In addition, this paper proposes a new lane changing model capable of handling both passive and active drivers' driving behavior for better performance of simulations. The proposed lane changing model was tested with Hanyang Simulatin (HYTSIM), a microscopic freeway simulation program developed for this study. The HYTSIM simulation results reflecting the performance of the proposed lane changing model were compared against the field data. The test results showed that the distribution of gaps collected when vehicles change lanes were statistically identical to the field data at 95% confidence level.

Computation of Maintainability Index Using SysML-Based M&S Technique for Improved Weapon Systems Development (SysML 기반 모델링 및 시뮬레이션 기법을 활용한 무기체계 정비도 지수 산출)

  • Yoo, Yeon-Yong;Lee, Jae-Chon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.11
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    • pp.88-95
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    • 2018
  • Maintainability indicates how easily a system can be restored to the normal state when a system failure occurs. Systems developed to have high maintainability can be competitive due to reduced maintenance time, workforce and resources. Quantification of the maintainability is possible in many ways, but only after prototype production or with historical data. As such, the graph theory and 3D model data have been used, but there are limitations in management efficiency and early use. To solve this problem, we studied the maintainability index of weapon systems using SysML-based modeling and simulation technique. A SysML structure diagram was generated to simultaneously model the system design and maintainability of system components by reflecting the maintainability attributes acquired from the system engineering tool. Then, a SysML parametric diagram was created to quantify the maintainability through simulation linked with MATLAB. As a result, an integrated model to account for system design and maintainability simultaneously has been presented. The model can be used from early design stages to identify components with low maintainability index. The design of such components can be changed to improve maintainability and thus to reduce the risks of cost overruns and time delays due to belated design changes.