• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.116-116
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• 2003

명천산, 카마이시산, 나루산, 풍남산, 기장산, 울산산 미역(Undaria pinnatifida)의 성숙 포자체는 각각 2001년 5월에 명천 배양장에서 채묘하였다. 인공 채묘와 채묘의 주요방법은 손(1993)에 따랐다. 재배미역의 번식생태학과 생산량이 우수한 형질을 규명하기 위하여 2001년 12월부터 2002 4월까지 명천산, 카마이시산, 나루산, 풍남산, 기장산, 울산산 미역(Undaria pinnatifida)에 대해서 고흥군 명천 어장과 계도 어장에서 양식실험을 실시하였다. 형태적 형질에 있어서 명천, 계도 어장에서 전장은 3월까지 급격한 성장을 보였으나 4월 이후 성장이 점점 완만해 지면서 감소하였다. 품종간에도 전장의 성장도가 달리 나타났으며 그중 가장 큰 것은 가마이시산이었고 가장 작은 것은 울산산이었다. 경장은 3월까지 지속적인 증가를 보였으며 6품종중 1품종은 증감하였다. 품종별로 경장의 변화는 달리 나타났다. 각 어장별 품종별로 줄기길이는 대부분이 월별로 증가하다 4월부터 감소하였다. 그러나 명천산은 4월까지 증가 하였다. 성실엽장은 6품종 모두 4월까지 지속적인 증가를 보였으며 특히 풍남산과 가마이시산이 두 어장에서 크게 나왔으며, 울산산이 두 어장에서 가장 작게 나타났다. 명천 어장에서 6품종의 전장과 경장의 상관계수는 카마이시산이 가장 크게 나타났고, 울산산이 가장 작게 나타났다. 계도 어장에서도 카마이시산이 가장 크게 나타났으며 울산산이 가장 작에 나타났다. 전장과 줄기길이의 상관계수는 명천어장에서는 풍남산이 가장 크게 나타났고, 울산산이 가장 작게 나타났으며, 계도 어장에서는 카마이시산이 가장 크게 나타났고 울산산이 가장 작게 나타났다. 명천 어장에서 전장과 성실엽장의 상관관계를 보면 명천산의 상관 계수가 가장 크게 나타났으며 나루토산이 가장 작게 나타났다. 계도 어장에서는 카마이시산이 가장 크게 나루토산이 가장 작게 나타났다. 명천, 계도 어장에서 전장과 각 형질간 ANOVA 분석은 1%~5% 수준에서 품종별로 모두 유의성 있게 나타났다.

• Journal of Life Science
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• v.12 no.4
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• pp.496-503
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• 2002

To develop a yeast strain of Pichia pastoris producing an antibacterial peptide, we have attempted the expression and secretion of an insect defensin. The nucleotide sequences corresponding to mature defensin were chemically synthesized by 6 oligomers, assembled in vitro and the synthesized gene was identified by nucleotide sequencing. The prepro sequence of yeast mating factor $\alpha$1 and the defensin gene were recombined into a Pichia expression vector, pPIC9K. The resulting plasmid, pPIDE, was transformed into P. pastoris GSl15 and transformants selected on histidine-deficient minimal plates were tested for antibacterial activity against Micrococcus luteus. Four strains with different antibacterial activity were selected for further analysis. Southern hybridization and RT-PCR verified the defensin gene was maintained and transcribed in a host. Four strains were cultivated in YPD broth for 96 hours to compare cell growth and antibacterial activity, They showed no difference in cell growth, however, each strain showed different antibacterial activity pattern with culture time. The maximal activity was about 550 AU/ $m\ell$.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.340-341
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• 2000

제주도에 주 분포하는 소라, Batillus cornutus는 제주도 연안의 암초성 패류 중 가장 대표적인 패류이다. 그간 소라에 대하여는 TAC 적용등 자원관리를 위하여 여러 조사 및 연구등이 활발하게 보고되고 있으나, 연령형질이 없는 소라의 연령과 성장을 정확히 파악하기는 어려움이 많은 종이다. 따라서 연급군의 분리를 통한 소라의 성장 parameter를 추정하여 금후 합리적 자원관리를 위한 자원생물학적 특성치를 정립하기 위하여 성장식을 유도하였다. (중략)

• Korean Journal of Ichthyology
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• v.13 no.4
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• pp.237-247
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• 2001

Three species, Gymnogobius urotaenia, G. sp. 1 and G. sp. 2, which were collected at Hosanriver, Hosan-ri, Samcheock-si, Gangwon-do, Korea, were examined for study of the age and growth. There was no differences between sexes in growth (p>0.05) except in age 0 of G. urotaenia, However, there were differences among the three species (p<0.0001). namely, G. sp. 1 was longer by about 5~10 mm (SL) than the other two species in each age group, while G. sp. 2 was shortest in each age group: G. rotaenia, at age 0 was less than 45 mm, at age 1 to be 45~60 mm, at age 2 to be 60~80 mm, at age 3 to be over 80 mm, and its maximum size was 105.0 mm. G. sp. 1, at age 0 was less than 55 mm, at age 1 55~65 mm, at age 2 65~85 mm, at age 3 over 85 mm and its maximum size was 105.3 mm. G. sp. 2, 0 age was less than 40 mm, at age 1 40~55 mm, at age 2 55~75 mm, at age 3 over 75 mm and its maximum size was 85 mm. Changes of body parts with increasing of body length were examined and analyzed. Covariance analysis showed interspecies differences in the following characters: body depth, head length, caudal peduncle depth, upper jaw length and pelvic fin ray length. G. urotaenia had the highest K-value (the relative growth coefficient) in head length and pelvic fin ray length, and G. sp. 1 had the highest in body depth, caudal peduncle depth and upper jaw length. However, G. sp. 2 had the lowest K-value in these 5 characters.

• Journal of Life Science
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• v.17 no.2 s.82
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• pp.293-297
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• 2007

Green fluorescent protein (GFP) is an attractive reporter for bioprocess monitoring. A fluorescence-based method was developed to quantify GFP levels in transgenic plants and protein extracts. In this study, GFP was produced and secreted from suspension cells derived from transgenic rice. The RAmy3E promoter placed before the GFP gene controlled by sugars such as sucrose. The effects of sucrose concentration on the secretion of GFP and total protein into the medium were investigated in batch suspension culture. It was possible, therefore, to induce the expression of the GFP by removing sucrose from the cultured media or by allowing the rice suspension cells to deplete sucrose catabolically. The dry cell weight (7.06 g/L) and GFP level were detected as highest at 12%, 3% sucrose after 20 day culture, respectively. However secreted GFP fluorescence at the other sucrose concentrations (6%, 12%, 18% and 24%) were a little amount in media.

• Journal of Embryo Transfer
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• v.21 no.4
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• pp.273-280
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• 2006

MC4R, PRKAG3, FABP3, and ESR have reported as important candidate genes related to some economic traits in pigs. To investigate the association between these genes and economic traits, the analysis of restriction fragment length polymorphism (RFLP) was conducted on 147 individuals (96 Durocs and 86 Korea native pigs; KNP) using single nucleotide polymorphism (SNP). Different genotype frequencies of 4 candidate genes were observed in Duroc and KNP. There were significant associations between MC4R polymorphic site and average daily gain (ADG, p<0.05) and backfat thickness (BF, p<0.05) in the Duroc, ADG (p<0.05) and days to 70 kg (p<0.05) in KNP. PRXAG3 polymorphic site were significantly .elated to BF (p<0.05) in the Duroc, ADG (p<0.05) and days to 70 kg (p<0.05) in the KNP. In FABP3, association with BF (p<0.05) in the Duroc, ADG (p<0.05) and days to 70 kg (p<0.05) in the KNP were found. ESR polymorphic site was not significantly associated to any other traits.

• Korean Journal of Ichthyology
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• v.20 no.2
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• pp.81-89
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• 2008

An experiment was conducted for 80 days to determine the effect of feeding and starvation on the survival, growth, gonad-somatic index (GSI), hepato-somatic index (HSI), viscera index (LSI), the morphometric changes and hematological parameter in a cultured catfish, Silurus asotus. The survival and growth of the starved group was lower than those of the fed group during the experiment. Starvation resulted in retardation of growth, which provides examples of the reduction of final body weight, growth rate of body weight, specific growth rate and condition factor, whereas the fed group continued to grow and remained in good condition. Blood analysis (cortisol, glucose, aspartate aminotransferase, alanine aminotransferase, red blood cell and hemoglobin) showed significant differences between the fed and starved groups at the end of the experiment. These results suggest that all nutritional parameters used for starvation and feeding in this study appear to be a useful index of nutritional status in the cultured catfish.

• Korean Journal of Poultry Science
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• v.42 no.4
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• pp.315-325
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• 2015

Recently, livestock breeding is more focused on the meat quality rather than meat quantity, mainly due to the improvement of consumers' income. Among the meat quality traits, meat color is one of very important traits because meat color is the first selection criterion from the consumers in the market. Most of the economically important traits have continuous variations and these are called quantitative traits. the genomic locations affecting these traits are called quantitative trait locus (QTL), which is mostly controlled by many genes having small effects. In this study, the recent QTL and candidate gene studies were reviewed in order to meet the consumers' demand for the future market. In the chicken QTL database, three traits are related with meat colors, namely breast color (Bco), meat color (Mco), drip loss (DL) and pH. The identified number of QTLs is 33 from 13 chromosomal regions. In these QTL regions, 14 candidate genes were identified; Eight for meat color (APP, BCMO1, COL1A2, FTO, KPNA2, PSMD12, G0S2, FTSJ3), two for drip loss (AGRP, FTO) and four for pH (GALNT1, PCDH19, DIAPH1, SPP2). These QTLs and candidate genes need to be confirmed and fine mapping is ultimately needed for identification of causative variations. The recently developed chicken resource population using Korean native chicken can be used for the improvement of meat quality traits, which increase the value that needed in the chicken industry.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.24 no.1
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• pp.66-72
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• 1979

interrelationship among the characters of the top and the root within a plant was investigated by deviding the roots of the plant into two parts and growing each one in two different environmental conditions, The roots grown on the poor environmental conditions such as over flooding moisture and overdried were less in numbers of root. shorter in root length. and lighter in root weight than those of good growing conditions, It was demonstrated that the roots grown under the poor environmental conditions affected not only the growing of the associated tillers but also the growing of all the tillers within a plant. In comparision with untreated plants. the culm length of treated plants was shortened. and the heading time of them was delayed. The most remarkable yield reduction was appeared at the over flooding moisture treatment. and the next was at the overdried treatment. The ten dency of yield reduction was more severe in sandy soil than that of clay loam.

• The Science & Technology
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• v.32 no.10 s.365
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• pp.22-23
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• 1999

돼지를 이용한 형질전환동물 개발연구로 주목을 끌고 있는 건국대 축산학과 이훈택교수는 복제동물의 산업화, 특히 이들 동물을 이용한 이식장기 생산동물의 개발에 주력하고 있다. 산양의 성장호르몬 유전자를 이용한 형질전환돼지 생산에 성공한 바 있는 이교수는 돼지에서 미성숙 정자에 의한 수정을 성공시켜 앞으로 남성 불임환자 치료기술을 개발하는데도 폭넓게 활용될 것으로 기대된다.