• Title/Summary/Keyword: 섬유 분포 특성

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Identification by 16S rDNA Partial Sequencing of Thermophilic Bacteria with Fermentation of Pig Manure (16S rDNA 증폭에 의한 부분염기서열을 이용한 분뇨 발효 관련 고온 호기성 박테리아의 동정)

  • Kim, Myung Kil;Choi, Don Ha;Choi, In Gyu;Kim, Byung Gyu;Song, Jae Gyung
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.1
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    • pp.68-78
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    • 2006
  • The purpose of this study was to estimate the identification of thermophilic hacterial with fermentation of pig manure. To identify the characters of thermophilic bacteria related to fermentation at a high temperature condition, we selected 28 different kinds of original settling thermophilic bacteria that were sampled at different 23 areas. They were distributed 1$1{\times}10^5{\sim}10^8CFU$ at medium and the enzyme activity at $55^{\circ}C$ incubation condition, especially cellulase and a-amylase, were higher than those of $30^{\circ}C$. Partial sequencing data for 165 rDNA region were obtained from 28 samples representing 15 different genera. Bacillus subcilis, one of those bacteria, has endodermic spores at high fermented condition.

The Effect of Circulat Hole Size and Distribution on Strength of Braided Composite (브레이드 복합재료의 원공의 크기와 분포가 재료강도에 미치는 영향)

  • Lee, Gyeong-U;Gang, Tae-Jin
    • Korean Journal of Materials Research
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    • v.4 no.3
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    • pp.253-258
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    • 1994
  • The effect of hole size and hole-to-hole distance in the braided and laminated composite was studied in terms of tensile strength, pin bearing strength, and flexural strength of S2-glass fiber braided polyester. The tensile strength reduction with hole size was well fitted with he Whitney and Nuismer's prediction for the laminated composite. The characteristic distance was measured to be about 1.6mm for braided composite and 1.8mm for laminated one. The effect of distance between the centers of two circu lar holes on tensile strength was negligible when the distance between these two holes was larger than 4 times of the diameter of circular hole for both braided and laminated composite. The side effect was diminished when the center of hole was located 3 times farther than the diamet.er of the hole. The pin bearing strengths was decreased with the size of pin hole for both braided and laminated composite.

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도정도를 달리한 백미 및 미강에 함유된 phenolic acids와 총 폴리페놀 함량

  • 김성란;하태열;이지연;이세은;이현유
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.10a
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    • pp.226.1-226
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    • 2003
  • 최근 쌀 소비량이 감소되고 쌀중심 식생활의 영양적 우수성을 인식하지 못하여 쌀은 주식으로서 위치가 흔들리고 있다. 그동안 쌀의 영양특성에 관한 연구로서 주로 탄수화물 급원으로서의 당질에 관한 연구와 미강을 중심으로한 식이섬유, 미강유등에 관한 연구결과들이 발표되었다. 특히 미곡 부산물인 미강으로부터 다양한 유효성분들이 확인되어 그 효능이 보고되고 있으며 일부가 기능성 소재로서 제품화되기도 하였다. 그러나 실제 우리의 식생활에서 섭취되고 있는 백미 또는 현미상태에서의 유효성분의 분포 및 함량에 대한 연구는 미진한 상태이다. 본 연구에서는 쌀의 영양적우수성을 규명하고자 유효성분 중 phenolic acid와 폴리페놀 함량을 품종 및 도정도에 따라 조사하였다. 조생종인 오대와 만생종인 남평 품종으로부터 현미와 7분도, 10분도 및 12분도로 도정도를 달리한 백미, 도정도별 미강을 각각 제조하였다. Ferulic acid 등 쌀과 미강에 존재하는 phenolic acid 는 알칼리로 추출한 후 pH를 조정하고 에틸아세테이트로 반복 추출하여 HPLC로 분석하였다. 현미에 함유된 phenolic acid는 ferulic acid가 가장 많아 50% 내외를 차지하였으며 p-coumaric acid, benzoic acid, sinapinic acid 순으로 검출되었다. 오대 현미의 총 phenolic acid 함량은 65.9 mg%로서 남평 현미의 57.2 mg%보다 높았으며 도정도가 증가할수록 백미 중의 총 phenolic acid 함량은 감소되었다. 미강 중에는 백미의 10배 량에 해당하는 phenolic acid가 검출되었고 benzoic acid와 m-hydroxy benzoic acid는 미강 시료에서만 검출되었다. 도정한 백미 중의 phenolic acid는 28.8∼51.7 mg%, 미강에서 321.4∼438.4 mg% 범위로 나타났다. 현미, 백미 및 미강에 함유된 총 폴리페놀의 함량을 표준 페놀화합물로 카테친을 사용하고 비색법에 의하여 측정하였을 때 오대 현미의 폴리페놀 함량은 78.4 mg%, 남평 현미 88.8 mg% 였다. 도정한 백미 중의 총 폴리페놀 함량은 30.3∼56.9 mg%, 미강이 541.5∼472.6 mg%의 범위였다. 이상과 같이 쌀에는 phenolic acid 및 총 폴리페놀이 상당량 함유되어 있으며 특히 배유보다는 강층에 많이 존재하므로 이들 성분의 효율적인 이용을 위한 쌀의 섭취방안이 필요한 것으로 나타났다.

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Chungkook-jang Koji Fermentation with Rice Straw (볏짚을 이용(利用)한 청국장 제조(製造)에 관(關)한 연구(硏究))

  • Kim, Kyung-Ja;Ryu, Myung-Ki;Kim, Sang-Soon
    • Korean Journal of Food Science and Technology
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    • v.14 no.4
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    • pp.301-308
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    • 1982
  • Chungkook-jang Koji was fermented with rice straw at $40^{\circ}C\;and\;50^{\circ}C$ for 72 hours. The changes of proximate composition, pH, titrable acidity, nitrogen compounds, protease activity and free-amino acids during the fermentation were investigated. Moisture, lipid and protein contents remained essentially unchanged during the fermentation. The pH was gradually increased from 6.4 to 7.46 and 7.82 at $40^{\circ}C\;and\;50^{\circ}C$, respectively, after 72 hour fermentation. Amino type and water soluble nitrogen increased as fermentation progressed. however, the former slightly decreased after 60 hour fermentation. Chungkook-jang fermented at $40^{\circ}C$ showed somewhat higher protease activity than $50^{\circ}C$. However, protease activity at both fermentation temperatures showed the same trend; that is, it increased until 48 hour fermentation and thereafter decrease. Free amino acid content of Chung-kook-jang after 72 hour fermentation at $40^{\circ}C$ was 6 times greater than that of the steamed soybean, while it was 2.5 times greater at $50^{\circ}C$. Based on these results. it seems that the optimum fermentation conditions for Chungkook-jang were $40^{\circ}C$ and 72 hours.

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Root Starch Yield and Physio-Chemical Characteristics of Bracken (Pteridium aquilinum Kuhn) according to Harvesting Time (고사리 뿌리의 채취시기에 따른 전분 추출수율과 이화학적 특성)

  • Jung-Seob Moon;Gue-Saeng, Yeom;Song-Hee Ahn;;Dong-Chun Cheong
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.74-74
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    • 2020
  • 고사리(Pteridium aquilinum Kuhn)는 고사리 속(Pteridium spp.)에 속하는 양치식물의 총칭으로서 우리나라에는 22과 70속 272종이 분포되어 있는 것으로 보고되었다. 고사리의 어린 순에는 가식부 100g 당 칼슘 15.0mg, 칼륨 185.0mg 등이 함유되어 있으며 골다공증, 심혈관 질환 등에 효과가 있고 식이섬유로 인해 변비 예방에도 유용한 것으로 알려져 있다. 우리나라의 고사리 재배면적은 2019년 기준 5,349ha 수준을 보이고 있으며 전체 산채류 재배면적에서 26%의 비중을 차지하고 있으나 재배면적의 증가에 따라 고사리 재배의 부가가치를 높일수 있는 방안이 요구되고 있는 실정으로 그 중 고사리 뿌리에서 추출한 전분은 중국 및 일본 등지에서 면류나 제과용으로 이용되고 있어 고사리 재배의 부가가치를 향상시킬 수 있는 방안으로 기대되고 있다. 본 연구에서는 고사리 뿌리의 채취시기에 따른 뿌리 전분의 생산성과 이화학적 특성을 조사하기 위해 2020년 3월 26일부터 10월 28일 까지 5회에 걸쳐 뿌리를 수확하여 세척한 후 고무망치로 파쇄하고 수침 방법을 이용해 추출한 전분의 추출수율과 이화학적 특성을 조사하였다. 채취시기별 고사리 뿌리의 생산성은 7월 27일 수확에서 3,783.3kg/10a로 유의하게 높은 수량을 보였다. 파쇄한 고사리 뿌리를 24시간 sodium metabisulfite 0.4% V/W 용액에 수침하여 전분을 추출한 후 24시간 간격으로 3회에 걸쳐 진탕, 침전과 세척의 과정을 거친 후 조사한 전분 추출 수율은 3월 26일 수확한 뿌리에서 7.6%로 가장 높았고 7월 27일과 9월 24일 채취한 경우에서는 전분이 검출되지 않았으며 10월 28일에는 4.9%의 유의하게 낮았다. 추출한 전분의 색도분석에서 L값은 채취시기가 늦어질수록 높아지는 경향을 보였고 a, b값은 낮아지는 경향을 보여 전분의 색도가 향상되는 경향을 보였으며, 신속점도측정기로 조사한 전분의 호화특성에서는 채취시기가 늦어질수록 전분의 최고점도는 높아졌으나 최저점도 및 최종점도는 낮아지는 경향을 나타냈다. 전분의 무기물 함량은 3월 26일과 5월 25일 채취하는 경우 Fe > Ca > Mg > K > P의 순으로 함량이 높았으나 10월 28일 채취한 경우에서는 Fe와 Ca이 감소되고 K 함량은 증가하여 Ca > Fe > Mg > K > P의 순으로 무기물 함량이 높게 나타났다.

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Anisotropy Measurement and Fiber Tracking of the White Matter by Using Diffusion Tensor MR Imaging: Influence of the Number of Diffusion-Sensitizing Gradient Direction (확산텐서 MR 영상을 이용한 백질의 비등방성 측정 및 백질섬유 트래킹: 확산경사자장의 방향수가 미치는 영향)

  • Jun, Woo-Sun;Hong, Sung-Woo;Lee, Jong-Sea;Kim, Sung-Hyun;Kim, Jae-Hyoung
    • Investigative Magnetic Resonance Imaging
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    • v.10 no.1
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    • pp.1-7
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    • 2006
  • Purpose : Recent development of diffusion tensor imaging enables the evaluation of the microstructural characteristics of the brain white matter. However, optimal imaging parameters for diffusion tensor imaging, particularly concerning the number of diffusion gradient direction, have not been studied thoroughly yet. The purpose of this study was to evaluate the influence of the number of diffusion gradient direction on the fiber tracking of the white matter. Materials and methods : 13 healthy volunteers (ten men and three women, mean age 30 years, age range 23-37 years) were included in this study. Diffusion tensor imaging was performed with different numbers of diffusion gradient direction as 6, 15, and 32, keeping the other imaging parameters constant. The imaging field ranged from 1 cm below the pons to 2-3 cm above the lateral ventricle, parallel to the anterior commissure-posterior commissure line. FA (fractional anisotropy) maps were created via image postprocessing, and then FA and its standard deviation were calculated in the genu and the splenium of the corpus callosum on each of FA maps. Fiber tracking of the corticospinal tract in the brain was performed and the number of the reconstructed fibers of the tract was measured. FA, standard deviation of FA and the number of the reconstructed fibers were compared statistically between the different diffusion gradient directions. Results : FA is not statistically significantly different between the different diffusion gradient directions. By increasing the number of diffusion gradient direction, standard deviation of FA decreased significantly, and the number of the reconstructed fibers increased significantly. Conclusion : The higher number of diffusion gradient direction provided better quality of fiber tracking.

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Coexpression of $P2X_3$ with TRPV1 in the Rat Trigeminal Sensory Nuclei (흰쥐 삼차신경감각핵에서 $P2X_3$와 TRPV1의 공존에 관한 연구)

  • Moon, Yong-Suk;Ryoo, Chang-Hyun;Cho, Yi-Sul;Kim, Hong-Tae;Park, Mae-Ja;Paik, Sang-Kyoo;Moon, Che-Il;Kim, Yun-Sook;Bae, Yong-Chul
    • Applied Microscopy
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    • v.38 no.3
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    • pp.151-157
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    • 2008
  • Trigeminal primary afferents expressing $P2X_3$ or transient receptor potential vanilloid 1 (TRPV1) are involved in the transmission of nociceptive information. In order to characterize $P2X_3$- and TRPV1-immunopositive neurons in the trigeminal ganglion (TG) and trigeminal caudal nucleus (Vc), we performed immunofluorescence experiments using anti-$P2X_3$ and anti-TRPV1 antisera and a morphometric analysis. 77.4% (1,401/1.801) of all the $P2X_3$-postive neurons coexpressed TRPV1 and 51.9% (1,401/2,698) of all the THFV1-immunopositive neurons also costained for $P2X_3$ in the TG. Immunoreactivity for both $P2X_3$ and TRPV1 were present in medium-sized neurons but not in small- and large-sized neurons. $P2X_3$ and/or TRPV1-immunopositive fibers were observed in the primary afferents and their associated axons in the Vc. These fibers and terminals were distributed in the superficial lamina of Vc: $P2X_3$-immunopositive fibers and terminals were distributed in the lamina I and II, expecially in the inner part of lamina II (lamina IIi), whereas TRPV1-immunopositive ones were densely detected in the lamina I and outer part of lamina II (lamina IIo). Immunopositive fibers and terminals for both $P2X_3$ and TRPV1 were observed on the border between lamina IIi and IIo. These results suggest that terminals coexpressing $P2X_3$ and TRPV1 are involved in specific roles in the transmission and processing of orofacial nociceptive information.

Sensory Characteristics of White and Black Sesame Gruels with Different Mixing Ratio and Decortication (깨의 함량과 전처리에 따른 깨죽과 흑임자죽의 기호도 연구)

  • 김진숙;손정우;염초애
    • Korean journal of food and cookery science
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    • v.12 no.4
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    • pp.547-556
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    • 1996
  • The purpose of this study was to invesitage the optimum cooking method, which ingridients mixing ratio and decortication of white and black sesame gruels, were examined by sensory and mechanical tests. As the results of sensory evaluation for the decortication white and black sesame gruels with the levels of 50, 100, 150, and 200% sesame per rice l00g, the flavor, nutty taste, and the overall preference were increased with the increase of sesame add. The gruel which made with decorticated sesame roafting for 7 minutes was proferable. The gruels 150% decorticated white and black sesame roasted 7 min had better overall preference. In the mechanical measurement for the white sesame gruels, L value tended to be decreased, while ‘a' and ‘b' values as well as viscosity tented to be increased as the sesame ratio and roasting time increased. In black sesame gruels, L value tended to be decreased, while ‘a' and ‘b' values as well as viscosity tended to be increased when sesame ratio was increased. In correlation between sensory evaluation and mechanical measurement of the white and black sesame gruels, color, flavor, nutty taste of sensory evaluation were correlated with the mechanical measurement. The optimal material mixing ratio for gruels was rice 100 g, white and black sesame 150 g, and water 1075 g.

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Anatomical Studies of Major Tree Barks Grown in Korea - II. Anatomy of Quercus Barks (한국산(韓國産) 주요수피(主要樹皮)의 해부학적(解剖學的) 연구(硏究) - 제2보(第二報) 참나무속(屬) 수피(樹皮)의 해부(解剖))

  • Lee, Hwa-Hyoung;Lee, Phil-Woo
    • Journal of the Korean Wood Science and Technology
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    • v.5 no.1
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    • pp.3-8
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    • 1977
  • A bark comprises about 10 to 20 percents of a typical log by volume, and is generally considered as an unwanted residue rather than a potentially valuable resource. As the world has been confronted with decreasing forest resources, natural resources pressure dictate that a bark should be a raw material instead of a waste. The utilization of the largely wasted bark of genus Quercus grown in Korea can be enhanced by learning its anatomical structure and properties. In this paper, bark characteristics of Quercus grown in Korea are described. In bark anatomy, general features such as color of rhytidome, exfoliating form, color of periderm, arrangement of periderm, and thickness of the inner and outer hark. etc., arc discussed. Studies on the microscopic structure include sieve tube, companion cell, parenchyma, pholem fiber, ray, periderm(phelloderm, phelloogen, phellem), sclereid, and crystal, etc. The results may be summarized as follows: 1. In general characteristics of rhytidomes, exfoliating is not easy and sclereids are distint to the naked eye. Inner bark is thicker than that of outer bark except in case of Q. variabilis. 2. It is not clear to distinguish between phelloderm and phellogen in Quercus bark. The phellem is developed conspicuously in Q. variabilis but that of Q. accutissima is composed of thinwalled phellem and thickwalled stone cell. 3. Quercus Bark has sieve tube, companion cell, phloem fiber and sclereid. Sclereids of Quercus bark are the most distinguished characteristics comparing with pinus and populus. The volume percent of sclereids are higher than that of fiber. 4. Rays are 1~3 seriate, and multiseriate ranging with from 15 to 20. 5. Parenchyma cell contains two types, polygonal and druses crystal.

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Short-Term Efficacy of Steroid and Immunosuppressive Drugs in Patients with Idiopathic Pulmonary Fibrosis and Pre-treatment Factors Associated with Favorable Response (특발성폐섬유화증에서 스테로이드와 면역억제제의 단기 치료효과 및 치료반응 예측인자)

  • Kang, Kyeong-Woo;Park, Sang-Joon;Koh, Young-Min;Lee, Sang-Pyo;Suh, Gee-Young;Chung, Man-Pyo;Han, Jung-Ho;Kim, Ho-Joong;Kwon, O-Jung;Lee, Kyung-Soo;Rhee, Chong-H.
    • Tuberculosis and Respiratory Diseases
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    • v.46 no.5
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    • pp.685-696
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    • 1999
  • Background : Idiopathic pulmonary fibrosis (IPF) is a diffuse inflammatory and fibrosing process that occurs within the interstitium and alveolus of the lung with invariably poor prognosis. The major problem in management of IPF results from the variable rate of disease progression and the difficulties in predicting the response to therapy. The purpose of this retrospective study was to evaluate the short-term efficacy of steroid and immunosuppressive therapy for IPF and to identify the pre-treatment determinants of favorable response. Method : Twenty patients of IPF were included. Diagnosis of IPF was proven by thoracoscopic lung biopsy and they were presumed to have active progressive disease. The baseline evaluation in these patients included clinical history, pulmonary function test, bronchoalveolar lavage (BAL), and chest high resolution computed tomography (HRCT). Fourteen patients received oral prednisolone treatment with initial dose of 1mg/kg/day for 8 to 12 weeks and then tapering to low-dose prednisolone (0.25mg/kg/day). Six patients who previously had experienced significant side effects to steroid received 2mg/kg/day of oral cyclophosphamide with or without low-dose prednisolone. Follow-up evaluation was performed after 6 months of therapy. If patients met more than one of followings, they were considered to be responders : (1) improvement of more than one grade in dyspnea index, (2) improvement in FVC or TLC more than 10% or improvement in DLco more than 20% (3) decreased extent of disease in chest HRCT findings. Result : One patient died of extrapulmonary cause after 3 month of therapy, and another patient gave up any further medical therapy due to side effect of steroid. Eventually medical records of 18 patients were analyzed. Nine of 18 patients were classified into responders and the other nine patients into nonresponders. The histopathologic diagnosis of the responders were all nonspecific interstitial pneumonia (NSIP) and that of nonresponders were all usual interstitial pneumonia (UIP) (p<0.001). The other significant differences between the two groups were female predominance (p<0.01), smoking history (p<0.001), severe grade of dyspnea (p<0.05), lymphocytosis in BAL fluid ($23.8{\pm}16.3%$ vs $7.8{\pm}3.6%$, p<0.05), and less honeycombing in chest HRCT findings (0% vs $9.2{\pm}2.3%$, p<0.001). Conclusion : Our results suggest that patients with histopathologic diagnosis of NSIP or lymphocytosis in BAL fluid are more likely to respond to steroid or immunosuppressive therapy. Clinical results in large numbers of IPF patients will be required to identify the independent variables.

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