• Proceedings of the Korean Institute of Building Construction Conference
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• 2022.04a
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• pp.227-228
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• 2022

In order to achieve the goal of cost management, which is one of the three major management goals of building production, this paper introduces an approximate cost estimating automation technology in the design stage as the importance of predicting construction costs increases. BIM is used for accurate estimating, and the quantity of structural members and finishing materials is calculated by creating a 3D model of the actual building. However, only 2D basic design drawings are provided when making an estimating. Therefore, for accurate quantity calculation, digitization of 2D drawings is required. Therefore, this research calculates the quantity of concrete structural members by calculating the area for the recognition area through 2D drawing recognition technology incorporating computer vision. It is judged that the development technology of this research can be used as an important decision-making tool when predicting the construction cost in the design stage. In addition, it is expected that 3D modeling automation and 3D structural analysis will be possible through the digitization of 2D drawings.

• Proceedings of the Korean Society of Computer Information Conference
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• 2009.01a
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• pp.351-359
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• 2009

21 세기 초 경쟁 환경에서 조직이 대처하기 위한 기업환경의 패러다임도 바뀌어 가면서 지식자산은 보다 더 중요한 기업가치 창출의 요소로 자려 잡게 되었다. 그러나 아직까지 대다수의 기업은 지식경영을 수행함에 있어 조직문화나 정보기술과 같은 지식경영 영향요소 (knowledge management enabler)자체에만 초점을 맞추고 있을 뿐 이를 어떻게 전략적으로 활용할 것인가에 대한 명확한 체계를 갖고 있지 못한 것도 사실이다. 따라서 종사원들이 보유하고 있는 지식이 기업의 근본자산이 되는 호텔 기업경영에 있어 변화하는 환경에 능동적으로 대처하며, 또한 시장에서의 경쟁우위의 획득 및 유지를 위해서는 전략적 지식경영 하에서 기업의 지식경영활동이 이루어져야 한다. 이러한 관점에서 호텔기업의 지식경영전략이 경영성과에 미치는 영향을 분석한 결과 형식지를 중심으로 한 시스템 지향적 지식경영전략과 앙묵지를 중심으로 한 인적 지향적 지식경영전략 모두 경쟁우위 정 (+)의 영향을 미치는 것으로 나타났다. 또한 경쟁우위는 재무성과에 정(+)의 영향을 미치는 것으로 나타났다. 반면에 지식경영전략은 재무성과에 부(-)의 영향을 미치는 것으로 나타나, 지식경영전략은 호텔기업의 경쟁우위를 획득 유지시키며 이를 통해 재무성과에 영향을 미친다고 나타났다. 이는 호텔기업에 있어서 모든 지식자산(암묵지, 형식지)를 기반으로 한 지식경영의 중요성을 인식시키고, 보다 지식경영활동을 활성화함으로써 호텔기업의 기업성과를 제고시킬 수 있는 구체적인 실행방안을 제시하였다.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.65-72
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• 2004

Instability of some eukaryotic sequence propagated in prokaryotic hosts is a frequently observed phenomenon. It is well documented that long inverted repeats, AT-rich sequences with structures like Z-DNA are extremely unstable in E. coli. These sequences may either be under-represented or even lost when cloned in E. coli. When we analyzed the polymorphic pattern for several tandom repeat (TR) in human SCKI gene, we found some TR regions were frequently deleted from plasmids and had difficult problem for their sequencing. These regions may result in non-clonability of the DNA sequence. Here we have cloned two difficult TR regions under low temperature and made two library for DNA sequencing using a nebulizer or sonicator. This study will help to determine the unstable genomic elements in complex mammalian genome.

• Journal of Food Hygiene and Safety
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• v.8 no.3
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• pp.163-169
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• 1993

The acute toxicity of lAS-Na(Linear Alkylbenzene Sulfonate-Na) and MES (ASME, Alpha-Sulfo fatty acid Methyl Ester), surfactans, was eyaluated in ICR mice for 14 days. Mice aging 6 weeks were administered orally with 0, 1,000, 1,320, 1,780, 2,280, 3,000 mg/kg of lASNa or 0, 1,000, 1,560, 2,450, 3,830, 6,000 mg/kg of MES in saline. The body weight of the treated animals was not significantly different from the controls. The main clinical signs of animals treated with lAS-Na or MES were diarrhea, decreased motor acthity and piloerection. The congestion in small intestine was only gross finding in dead animals treated with two sulfactants. In this study, $LD_{50}$ values of lAS-Na and MES were eyaluated 1,319 mg/kg in male and 1,402 mg/kg in female mice, 2,040 mg/kg in male and 2,548 mg in female mice, respectiyely.

• Journal of the Korean Society of Tobacco Science
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• v.2 no.2
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• pp.8-13
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• 1980

The inhibition effect of flavone and butylatedhydroxyanisole (BHA) on the microsomal activation of Cigarette Smoke Condensate (CSC) or its Neutral Portion (NP) was investigated in Rat. The activities of Latic acid dehydrogenase (LDH) in serum was measured in the time intervals of 3, 6, 12, 18, 24 and 30 hr, respectively, after the injection (ip) of CSC (5mg/kg) or NP (10mg/kg) to Wistar male rat. Flavone (1mg/kg) and BHA (1mg/kg) were injected along with CSC or NP. The significant enhancement of the LDH activity in serum was observed in both cases of rats treated with CSC and NP. A drastic decrease of LDH activity from 1040 unit to 641 unit was observed after 12 hours of injection of CSC along with flavone. In contrast with the case of flavone, BHA reduced the enzyme activity from 825 unit to 652 unit at the same condition of flavone. Therefore, flavone can be considered to be a better inhibitor on action of CSC in vivo.

• Water for future
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• v.28 no.4
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• pp.215-222
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• 1995

The water retention function which has the hysteresis phenomena is required to analyze the Richards equation which is a governing equation of the unsaturated flow, and its hysteresis phenomena has influence upon the characteristics of the unsaturated flow. The accuracy of the published hysteresis models is compared by using experimental data of the water retention function. The apparatus to experiment the hysteresis phenomena on the soil is developed, and experimental data for the main wetting process and the main drying process of the water retention function are obtained. The parameters of the van Genuchten equation are calibrated by using experimentally obtained data. As a result of the comparison of the selected hysteresis models which simulate the main drying curve from the main wetting curve, the Model I-1(Mualem) overestimates and the Model II-1(Mualem) underestimates but the Model III-2(Park and Sonu) similarly estimates the experimental data of the main drying curve.

• Journal of Veterinary Clinics
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• v.27 no.4
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• pp.402-406
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• 2010

Bovine leukemia virus (BLV) is a delta-retrovirus which causes chronic lymphocytosis in cattle. BLV infections have been divided into two groups such as enzootic bovine leukosis (EBL) and sporadic bovine leukosis (SBL) according to the clinical symptoms in infected cattle. The conventional detection method of BLV was hematological procedure which is determining lymphocytosis in the suspected animals. Recently several sensitive methods were developed to detect antibody to BLV and nucleic acid of the BLV from infected cattle. In this study we have compared the difference of positive rates between agar gel immunodiffusion (AGID) and enzyme linked immunosorbent assay (ELISA) which are using for BLV antibody detection methods. The positive detection rate of ELISA test was 7.4% greater than the positive rate of AGID. The discrepancy of the positive rate between ELISA and AGID were showed in the group of age over one year old to under three year old group. The result from each test agreed very well in the group of over 5 year old cattles. The serological test is very useful method to select the infected cattle for the eradication or control of the disease in the infected herd. But it has a limit by interference of the maternal antibody from the cow of under 6 month old. This study shows that 16.2% of these ages group showed BLV gene positive by polymerase chain reaction (PCR) method. The result suggests that ELISA test need to be used with PCR to clarify misinterpretation of positive animals by antibody response due to the natural infection from maternally derived antibody in calves of under 6 months old.

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.96-102
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• 2002

The S-phase checkpoint mechanisms response to DNA damage or inhibition of DNA replication for maintenance of genetic stability in eukaryotic cells. These roles include cell cycle control arrest at S-phase and Iranscriptional induction of repair genes. To characterize the defects of dpbll mutant for both these responses, we examined the over-expression effect of DPBll gene, the sensitivity to HU, MMS, and the transcriptional pattern by DNA damage agent for RNRS mRNA. RNRS transcript is induced in response to a wide variety of agents that either damage D7A directly through chemical modification or induce stress by blocking DNA synthesis. As results, dpbll-1 cells are sensitive to DNA damage agents and the level of RNR3 mRNA is reduced approximately 40% than wild type cells. Moreover, we found the same results in dpb2-1 cells. Therefore, we propose that DPB2 and DPBll act as a sensor of replication that coordinates the transcriptional and cell cycle responses to replication blocks.

• YAKHAK HOEJI
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• v.54 no.1
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• pp.49-54
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• 2010

SLC6A19 which reported as a neurotransmitter was composed of seven minisatellites. In previous our study, the minisatellites variants of SLC6A19-MS7 showed the susceptibility for hypertension. When this minisatellte sequences were analyzed using the bioinformatic tool, USF1 (upstream transcription factor 1) was found in this region as a putative transcription factor binding site. USF1 is binding with E-boxes which has a consensus sequence of CACGTG. USF1 is a ubiquitously expressed transcription factor and involved in the transcriptional control of many genes including the molecular pathogenesis of cardiovascular disease. Thus, we investigated that the putative functional relationship between the minisatellites variants and susceptibility for myocardial infarction. A case-control study was performed that compared genomic DNA from 400 controls and 225 cases with myocardial infarction. There were no significant differences observed in the overall allelic distribution of minisatellites between controls and cases, which indicates that this polymorphism is not responsible for myocardial infarction susceptibility. Hence, we analyzed the five different minisatellites alleles from this study and characterized 14 different repeats units (Unit1~Unit14). Then, we evaluated the DNA composition, phylogenic tree, and pairwise distances of its repeats. The variability of each repeats differed from 2.33% to 16%. The phylogenic trees for the four SLC6A19-MS7 minisatellites exhibited very different shapes in their braches and distances, and present most common 8 repeats allele was the longest 14 repeats allele. Therefore, this result may help to understand for the evolutional level of the length of minisatellites.

• Journal of the Korean Institute of Landscape Architecture
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• v.14 no.2
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• pp.43-68
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• 1986

It is necessary that children's actual activities in the playgrounds should be analyzed for the study on the children's playgrounds. The purpose of this study is to establish the design principles for the improvement of the children\`s playgrounds in apartment complex by means of the various methods ; behavior mapping, questionaires and photo simulations. For the purpose of data collection, children are divided into three groups-infants(0 to 4 years), young children(pre ; 5 to 8 years, post ; 9 to 11 years), teenagers(7.2 to 14 years). The study results are as follows; 1) Analysis of behavior mapping : (a) Approximately 70 percent of the children observed stayed in the playground less than 20 minutes. (b) Playing time for each play apparatus was less than 5 minutes. (c) As a result of the observation, the duration time in the attic was 1 hour or more (d) The swing was the most frequently used apparatus as children's first and last choice. (e) Most of the users were young children(46.5%). 2) Analysis of questionaires : (a) Children considered that the existing playgrounds were lack of shadows and the play apparatuses are not interesting. (b) They felt danger and crowding at the multi-used wooden apparatus. 3) Suggestions for the playground design : It is necessary that the playgrounds are divided into the infants, the young children's and the teenagers'playground. (a) The infants'playground needs about 50 square meters and needs to be located in the apartment court. (b) The young children's playground needs about 330 to 660 square meters and needs to be located adjacent to the pedestrian road. (c) The teenagers'playground needs about 660 to 1000 square meters, which is needed for various ball games.