• Journal of Life Science
• /
• v.10 no.4
• /
• pp.422-430
• /
• 2000

In order to search for the effects of Shine-Dalgarno (SD) sequence and nucleotide sequence of spacer region (SD-ATG) on bGH expression, oligonucleotides containing random SD sequences and a spacer region were chemically synthesized. The distance between SD region and initiation codon (ATG) was fixed to 9 nucleotides in length. The expression vectors have been constructed using pT7-1 vector containing a T7 promoter. Positive clones were screened with colony hybridization and named pT7A or pT7B plasmid series. The selected clones were confirmed by DNA sequencing and finally, 19 clones having various SD combinations were obtained. When bovine growth hormone was induced by IPTG in E. coli BL21(DE3), all cells harboring these plasmids produced a detectable level of bGH in western blot analysis. However, various SD sequences did not affect on bGH expression, indicating that the sequences of SD and the spacer region did not sufficiently destabilize mRNA secondary structure of bGH gene. Therefore, these results indicate that the disruption of mRNA secondary structure might be a major factor for regulating bGH expression in the translational initiation process.

• Transactions of the Korean Society of Pressure Vessels and Piping
• /
• v.15 no.2
• /
• pp.31-39
• /
• 2019

Cracked component analysis is needed for structural integrity analysis under seismic loading. Under large amplitude cyclic loading conditions, the change in material properties can be complex, depending on the magnitude of plastic strain. Therefore the cracked component analysis under cyclic loading should consider appropriate cyclic hardening model. This study introduces a procedure for determining an appropriate cyclic hardening model for cracked component analysis. The test material was nuclear-grade TP316 stainless steel. The material cyclic hardening was simulated using the Chaboche combined hardening model. The kinematic hardening model was determined from standard tensile test to cover the high and wide strain range. The isotropic hardening model was determined by simulating C(T) test under cyclic loading using ABAQUS debonding analysis. The suitability of the material hardening model was verified by comparing load-displacement curves of cyclic C(T) tests under different load ratios.

• Proceedings of the Korean Information Science Society Conference
• /
• 2004.04b
• /
• pp.151-153
• /
• 2004

비즈니스 인텔리전스는 정보를 저장하고 분석하여 부가가치를 창출해 내는 기술로써 무한경쟁시대에서 기업이 경영환경 추세를 파악하고 경영전략을 세우는데 필수적인 도구이다. 비즈니스 인텔리전스 핵심기술로는 정보를 분석하기 쉬운 형태로 저장하는 데이터웨어하우징 기술과, 정보를 다차원적으로 분석하는 OLAP 기술, 그리고 데이터에 숨겨진 패턴과 관계를 찾아내는 데이터마이닝 기술이 포함되며, 이들 각각을 위한 소프트웨어 제품들은 개발되어 널리 사용되고 있다. 그러나 기업이 요구하는 분석결과를 적시에 제공하기 위해서는 이들을 통합한 개발 프레임워크가 필요하며, 본 연구팀은 그러한 프레임워크 개발 프로젝트(Chamois Project)에 참여하고 있다. 이 프레임워크에는 유사한 기능을 제공하는 여러 종류의 소프트웨어 제품들이 포함되어 있어서, 공통의 응용 프로그래밍 인터페이스(API)를 제공하는 것이 필요하다. 본 연구에서는 특히 다양한 OLAP 제품들을 수월하게 사용할 수 있도록 공통 OLAP API를 개발하였고, 이를 소개하고자 한다. 이와 같이 통합 환경에서 다양한 OLAP 제품들을 지원할 수 있다는 것은 OLAP 제품들의 선택의 폭을 넓히고 응용 프로그램의 코드를 수정하지 않아도 보다 나은 기능을 갖는 OLAP 제품으로의 업그레이드가 쉽게 이루어진다는 장점을 갖는다.

• Elastomers and Composites
• /
• v.40 no.2
• /
• pp.119-127
• /
• 2005

Material characteristics and lifetime evaluation are very important in design procedure to assure the safety and reliability of the rubber components. In this paper, the material test and accelerated heat aging test were carried out to predict the useful life of NBR and EPDM rubber mount for a compression motor which is used in a refrigerator. In order to investigate the effects of heat-aging on the material properties, crosslink density, modulus at 100% strain, stress-strain curves were obtained from uniaxial and equi-biaxial tensile tests. The change of compression set were used for assessment of the useful life and the time to threshold value were plotted against the reciprocal of absolute temperature to give the Arrhenius plot. The useful life at variable temperatures are obtained in the Arrhenius relationship.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.21 no.3
• /
• pp.51-57
• /
• 2021

In this paper, the relevance between Einstein's special theory of relativity (1905) and Bernoulli's fluid mechanics (1738), which motivates Shannon's theorem (1948), was derived from the AB=A/A=I dimension, and the Shannon's theorem channel code was simulated. When Bernoulli's fluid mechanics ΔP=pgh was applied to the Hallasan volcano Magma eruption, the dimensions and heights matched the measured values. The relationship between Einstein's special theory of relativity, Shannon's information theory, and the stack effect theory of fluid mechanics was analyzed, and the relationship between volcanic eruptions was mathematically proven. Einstein's and Bernoulli's conservation of energy and conservation of mass were the same in terms of bandwidth and power efficiency in Shannon's theorem.

• Horticultural Science & Technology
• /
• v.30 no.1
• /
• pp.93-96
• /
• 2012

'Double Shine', a cultivar of Gypsophila paniculata was newly developed for the cut flowers by Jeollabuk-do Agricultural Research & Extension Services. It has morphological characteristics of the spreading plant type, double florets of many petals and two pistils, emarginate petal tip and cup shaped calyx. Also 'Double Shine' has several traits such as strong flower stalk, bright colored flower, narrow branching angle and more or less resistance to powdery mildew and pest injury. Furthermore, 'Double Shine' produced higher primary branches, and longer flower stalk length with internodal length and than did a check variety 'Bristol Fairy' during summer cultivation in subalpine areas. Blooming of 'Double Shine' was delayed by 16 days, its floret had broader width, compared to 'Bristol Fairy'. Moreover, 'Double Shine' produced normal flowers with two pistils and 8.5 stamens, with little production of malformed flowers in the high temperature condition.

• Korean Journal of Environment and Ecology
• /
• v.26 no.5
• /
• pp.758-769
• /
• 2012

This study is aimed at analyzing the structural characteristics of vegetation in the Sangwonsa(Temple) valley, Yongmunsan(Mt.), and to collect basic data for research of large mountains which are located in the temperate, central region. The research on the entire vegetation struc trees are distributed over a wide area, accounting for 75.0% of theture of Sangwonsa(Temple) valley observed that oak total vegetation in the area. We examined 17 plots(unit area: $400m^2$) in the deciduous forest, dividing the trees into 5 community types based on the average important value. The 5 community types are as follows: A mixed dominant group of Carpinus laxiflora and Quercus serrata, a mixed dominant group of Quercus serrata and Quercus variabilis, a mixed dominant group of Quercus mongolica and Quercus serrata, a dominant group of Quercus serrata, a dominant group of Acer pictum supsp. mono. It is expected that the oak trees competing with Carpinus laxiflora and Acer pictum supsp. mono would flourish in a deciduous forest. The Shannon's species diversity index(H') was 0.3708~1.3083.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.41 no.9
• /
• pp.39-48
• /
• 2004

Turbo codes, whose performance in bit error rate is close to the Shannon limit, have been adopted as a part of standard for the third-generation high-speed wireless data services. Iterative Turbo decoding results in decoding delay and high power consumption. As wireless communication systems can only use limited power supply, low power design techniques are essential for mobile device implementation. This paper proposes new effective criteria for stopping the iteration process in turbo decoding to reduce power consumption. By setting two stopping criteria, decodable threshold and undecodable threshold, we can effectively reduce the number of decoding iterations with only negligible error-correcting performance degradation. Simulation results show that the number of unsuccessful error-correction can be reduced by 89% and the number of decoding iterations can be reduced by 29% on the average among 12500 simulations when compared with those of an existing typical method.

• Journal of Life Science
• /
• v.18 no.7
• /
• pp.907-911
• /
• 2008

Radish (Raphanus sativus L.) is one of very important crop plants in the world. It is very important to determine hybrid seed quality in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The allozyme for evaluating seed purity in a commercial $F_1-hybrid$ radish cultivar is demonstrated. Three hundred sixty seeds from the male and female harvest were subsequently screened for seed purity using 27 isozyme loci. Especially, F1 hybrids of radish, Per-1 ($aa{\times}bb$), Lap-1 ($aa{\times}bb$), Est-1 ($aa{\times}bb$) were presented clear hybrid bands. Est-1 locus revealed that 15 (8.3%) seeds from the female harvest and 26 (14.4%) seeds from the male harvest were sibs. It maintains higher than average level of genetic diversity compared with their correspondent parents. Shannon's index of phenotypic diversity (I) of hybrids was the highest of all accessions (R. sativus L. cv. Daepeng, R. sativus L. cv. Backza, and their hybrids). The allozyme may lead to a better insight into the hybrid seed purity.

• Journal of Korean Society for Quality Management
• /
• v.45 no.2
• /
• pp.227-246
• /
• 2017

Purpose: This study proposes a Shiny-Sigma Methods that combines the advantages of Six Sigma method and Shainin method in order to solve field defect. Each technique has advantages and disadvantages. Methods: This study proposed Shiny-Sigma by combining Six Sigma has the logical advantage of the problem solving road map and Shiny has the merits of finding the root problem from the defective phenomenon. The Six Sigma method has the disadvantage that it is difficult to solve if the number of data is small, but the Shiny method has the advantage of finding the root cause with a small number of data. Results: As a result of applying Shiny-Sigma method to the field, it has advantages of solving the problem easily and quickly than the existing Six Sigma method. It does not require a lot of statistical knowledge, which helps field workers to use it. Based on these successes L Co. company has obtained the effect of improving the production quality by applying the Shiny-Sigma method. Conclusion: The Shiny-Sigma method proved to be suitable for the production site as a result of field application. It is suitable for field workers with low statistical knowledge and is suitable for field where data is difficult to obtain. This method is not a method to solve all the problems because there are problems that can be solved according to the field problems. We hope that this method will spread to many industrial sites and this method will have a great effect on the improvement of field production quality.