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The Effect of Negative electric field using charged PTFE membrane on Bone Healing of Rabbit Long Bone (Charged membrane에 의한 negative electric field가 토끼 장골의 골 치유에 미치는 영향)

  • Kwon, Yong-Su;Park, Jin-Woo;Lee, Jae-Mok;Suh, Jo-Young
    • Journal of Periodontal and Implant Science
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    • v.34 no.3
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    • pp.551-562
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    • 2004
  • The purpose of this study was to evaluate the effects of negatively electric field on bone healing in rabbit segmental long bone defects using negatively charged PTFE membrane. Ten millimeter segmental defects in the rabbit radius were used as the experimental model. After membranes were then charge injected using a corona-charging apparatus, the left defects were covered with non charged PTFE membranes as control groups, whereas the right defect was covered with negatively charged PTFE membranes as test group. The animals were divided into 4 groups of 2 rabbits each, and sacrificed at 2, 4, 6, and 8 weeks. Histomorphometric analysis showed a more newly formed bone in negatively charged membrane at early healing period. At 2 weeks, the proportion of new bone formation to total defect area was 0.32% in control group, 1.10% in experimental group. At 4 weeks, the proportion of new bone formation to total defect area was 6.86% in control, and 13.75% in experimental. At 6 and 8 weeks, no obvious difference was found between the two groups but newly formed bone in test groups were slightly more than that in control groups. In conclusion, negatively charged membranes showed more newly bone tissue than noncharged membranes at an early healing period. Although the number of samples was small, this study showed that the combination of negatively electrical stimulation and P1FE membrane may be of value in long bone healing.

Study of a Recurring Anticyclonic Eddy off Wonsan Coast in Northern Korea Using Satellite Tracking Drifter, Satellite Ocean Color and Sea Surface Temperature Imagery (위성원격탐사를 이용한 동해 원산연안의 재발생 와동류 연구)

  • 서영상;장이현;김정희
    • Korean Journal of Remote Sensing
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    • v.16 no.3
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    • pp.211-220
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    • 2000
  • Even though recurring eddies at the terminal end of the East Korean Warm Current have been identified in the thermal infrared imagery from the NOAA/AVHRR sensor and ocean color data from Orbview-2/SeaWiFS sensor, it is difficult to make observation in the field regarding recurring eddies located around the Wonsan coastal area in North Korea. But we could get in situ data related to an eddy from an ARGOS satellite tracking drifter trapped in the eddy on January 4th, 1999. An ARGOS drifter, a NOAA satellite tracked buoy was trapped by the eddy during January 4th.March 18, 1999. The ARGOS drifter rotated 10 times per 72 days on the edge of the eddy located at $39^{\circ}N$, $129^{\circ}E$. The diameter of the eddy was about 100 km. The horizontal rotation velocity of the recurring cold-core anti-cyclonic eddy was 1.53 km/h(42 cm/sec). The sea surface temperatures of the eddy varied from $14.7^{\circ}C$ on January 5, 1999 to $9.6^{\circ}C$ on March 18,1999. To study the mechanism of the recurring eddy. we tried to find out the relationship between the vector of the drifter moving in the eddy and the wind vector in Sokcho and Ulleung Island located near the eddy in southern Korea, and the difference in sea level between Ulleung Island and Mukho. We hope the results of this study would be useful for calibration and validation data of simulation and numerical modeling studies of the recurring eddy.

Effect of Inorganic Environmental Factors on the Growth of Pinus koraiensis Seedlings (VII) -The Influence of Shading Pretreatment on the Weight Growth in Transplanting Bed- (무기적(無機的) 환경요인(環境要因)이 잣나무 유묘(幼苗)의 생육(生育)에 미치는 영향(影響)에 관(關)한 연구(硏究) (VII) -이식상(移植床)에서의 중량생장(重量生長)에 대(對)한 피음처리(被陰處理) 영향(影響)-)

  • Kim, Young Chai
    • Journal of Korean Society of Forest Science
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    • v.77 no.1
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    • pp.100-108
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    • 1988
  • This research was performed to estimate the influence of relative light intensity, planting density, growth period on the growth of fresh and dry weight of the stem and branches, and root in the pretreatment transplanting bed. 1. The growth of fresh and dry weight of the stem and branches per seedling varied in the order of 63%>100%>37%>19% in relative light intensity, and increased with decrease of planting density, and grew highly during the period from May to June. The contribution rates to the growth of fresh and dry weight of the stem and branches were 49.4% and 39.6% in shading treatment, and 4.3% and 6.4% in planting density, and 34.2% in growth period respectively. Of the three factors, that is, shading, density and period, all of the interactions between two factors, and among the three factors in the growth of fresh and dry weight of stem and branches were significant. The growth of fresh and dry weight of root per seedling increased with light intensity and decreased with increase of density. The best growth of root, showed during the period from May to June in fresh weight, but during the period from July and August in dry weight of root. For the three factors, that is, shading, density and growth period, all the interactions between any two factors, and among the three factors in the growth of fresh and dry weight of root were significant.

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Production of Chiral Phenyl Oxirane by Rhodosporidium toruloides in Hollow-fiber Reactor (Rhodosporidium toruloides를 이용한 Hollow-fiber 반응기에서의 광학활성 Phenyl Oxirane 생산)

  • 김희숙;박성훈;이은열
    • Journal of Life Science
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    • v.13 no.6
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    • pp.788-793
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    • 2003
  • Production of chiral phenyl oxirane by Rhodosporidium toruloides SJ-4 was investigated. Racemic phenyl oxirane was kinetically resolved by enantioselective hydrolysis reaction by epoxide hydrolase of R. toruloides in two-phase hollow-fiber reactor system. Dodecane with high concentration of the racemic substrate passed through the lumen side and cell suspension was recirculated through the shell side of the hollow fiber reactor For the removal of phenyl-1, 2-ethandiol to reduce the product inhibition to biocatalysts, another hollow-fiber reactor was employed to extract the diol. Racemic phenyl oxirane up to 300 mM was enantioselectively resolved with high enantiopurity (>99% ee) in hollow-fiber reactor system.

Determination of Ultimate Biodegradability and Multiple Decay Rate Coefficients in Anaerobic Batch Degradation of Organic Wastes (유기성폐기물의 회분식 혐기성 최종생분해도와 다중분해속도 해석)

  • Kang, Ho;Shin, Kyung-Suk;Richards, Brian
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.5
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    • pp.555-561
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    • 2005
  • A new graphical method was developed to separate two distinctive decay rate coefficients($k_1$ and $k_2$) at their respective degradable substrate fractions($S_1 and $S_2$). The mesophilic batch reactor showed $k_1$ of $0.151\;day^{-1}$ for wasted activated sludge(WAS), $0.123\;day^{-1}$ for thickened sludge(T-S), $0.248{\sim}0.358\;day^{-1}$ at S/I ratio of $1{\sim}3$ for sorghum and $0.155{\sim}0.209\;day^{-1}$ at S/I ratio $0.2{\sim}1.0$ for swine waste, whereas their long term batch decay rate coefficients($k_2$) were $0.021\;day^{-1}$, $0.001\;day^{-1}$, $0.03\;day^{-1}$ and $0.04\;day^{-1}$ respectively. At least an order of magnitude difference between $k_1$ and $k_2$ was routinely observed in the batch tests. The portion of $S_1$, which degrades with each $k_1$ appeared 71% for WAS, 39% for T-S, 90% for sorghum, and $84{\sim}91%$ at S/I ratio of $0.2{\sim}1.0$ for swine waste. Ultimate biodegradabilities of 50% for WAS, 40% of T-S, $82{\sim}92%$ for sorghum, and $81{\sim}89%$ for swine waste were observed.

The Anaerobic Biodegradability and Methanogenic Toxicity of Pulping Wastewaters (펄프페수의 혐기성 생분해능 및 메탄 생성균의 독성에 관한 연구)

  • 박종안;허준무;장봉기
    • Journal of Environmental Health Sciences
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    • v.24 no.1
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    • pp.70-79
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    • 1998
  • 본 실험의 목적은 다양한 펄프제조 조건과 여러 형태의 리그노 셀루로우스 성분이 펄프폐수의 혐기성 생분해에 미치는 영향을 규명하는 것이다. 실험에 사용된 폐수는 일반적으로 펄프 제조시에 발생되는 폐수를 대상으로 하였으며, 펄프제조 조건은 TMP공정과 소다 펄프공정을 적용하였다. 혐기성 생분해 가능성 시험 및 독성실험은 $35\pm 2\circ$C의 중온상태에서 입상슬러지를 식종물질로 사용한 회분식 반응조를 이용하였다. TMP공정의 배출되는 폐수는 산으로의 전환율이 총 COD기준으로 68-87%로 매우 높은 혐기성 생분해 가능성을 보였다. 그리고 TMP공정폐수는 일반적으로 제지폐수 처리시 독성농도라고 알려진 농도에서도 메탄생성균에 독성을 주지 않았고, 또한 COD 10g/l의 농도에서도 처리에 저해가 일어나지 않았다. 반면에, 알카리성 상태에서 준비된 펄프폐수의 경우는 생분해성이 매우 낮아서 대략 50%정도의 산전환율을 보였으며, 메탄생성균에 상당한 저해를 주었다. 메턴생성균의 활성도에 50%저해를 주는 농도는 2.1~5.4 gCOD/l였다. 알카리성 펄프폐수의 독성에 대한 추가 실험결과 펄프내 wood resin이 산이나 중성 pH부근에서는 잘 용해가 되지 않고 알카리성 상태에서 쉽게 용해되어 메탄생성균에 저해를 나타내는 것으로 밝혀졌다. 따라서 펄프제조시 나무성분이 알카리성분과 접촉할 경우 후속하는 혐기성 처리공정의 메탄생성균에 심각한 저해를 줄 수 있다.

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Zymomonas mobilis에 의해 생성된 Fructan (Levan)의 특성 및 화장품 원료로의 개발

  • 이재섭;양은경;이정하;김철호;박수남;이종원;김기호
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.28 no.1
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    • pp.186-201
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    • 2002
  • Fructan(Levan)은 식물체 및 미생물에서 발견되는 탄수화물로 이는 과당(fructose)이 $\beta$-2, 6 결합으로 연결되어 있는 polysaccaride 이다. 본 연구에서는 Fructan을 생성하는 미생물(Zymomonas mobilis)과 10% sucrose(기질), 1-2% 효모 추출물을 주성분으로 하는 배지를 사용하여 30-37$^{\circ}C$, pH 5.0-7.0에서 20-24시간 동안 배양한후 원심분리하여 균체를 제거하고 3배량의 알코올을 가하여 침전, 건조하여 얻은 Fructan의 화장품 원료로서의 가능성을 조사하였다. 보습효과에 있어서는 Hyaluronic acid와 유사하였으며, keratinocyte에 대한 세포증식 효과를 나타내었다. 또한 3-D culture에 의해 구축된 생인공 피부내에 0.05%의 sodium lauryl sulfate (SLS)를 사용하여 피부자극에 의한 초기 염증 반응을 유도한후 0.01mg/m1, 0.05mg/m1의 Fructan을 각각 처리하였을 때, SLS만을 처리한 생인공피부와 비교하여 세포증식효능을 보였고, SLS 자극물질로 유도된 전염증성 조절인자인 interleukin-l$\alpha$(IL-l$\alpha$)의 분비량을 조사 하였을때 0.01mg/ml, 0.05mg/ml의 Fructan을 처리한 생인공피부의 IL-l$\alpha$ 양이 Fructan을 처리하지 않은 것보다 상대적으로 감소하였다. 이러한 결과로 Fructan이 생인공 피부내 피부 세포의 증식효과를 나타낼 뿐만 아니라, 또한 피부자극물질에 의한 염증반응에 대해 자극완화효능이 있음을 알 수 있었다. 섬유아세포 및 동물을 이용한 안전성 시험에서도 독성이 없는 안전한 원료로 평가되었다.

Improving A Text Independent Speaker Identification System By Frame Level Likelihood Normalization (프레임단위유사도정규화를 이용한 문맥독립화자식별시스템의 성능 향상)

  • 김민정;석수영;정현열;정호열
    • Proceedings of the IEEK Conference
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    • 2001.09a
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    • pp.487-490
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    • 2001
  • 본 논문에서는 기존의 Caussian Mixture Model을 이용한 실시간문맥독립화자인식시스템의 성능을 향상시키기 위하여 화자검증시스템에서 좋은 결과를 나타내는 유사도정규화 ( Likelihood Normalization )방법을 화자식별시스템에 적용하여 시스템을 구현하였으며, 인식실험한 결과에 대해 보고한다. 시스템은 화자모델생성단과 화자식별단으로 구성하였으며, 화자모델생성단에서는, 화자발성의 음향학적 특징을 잘 표현할 수 있는 GMM(Gaussian Mixture Model)을 이용하여 화자모델을 작성하였으며. GMM의 파라미터를 최적화하기 위하여 MLE(Maximum Likelihood Estimation)방법을 사용하였다. 화자식별단에서는 학습된 데이터와 테스트용 데이터로부터 ML(Maximum Likelihood)을 이용하여 프레임단위로 유사도를 계산하였다. 계산된 유사도는 유사도 정규화 과정을 거쳐 스코어( SC)로 표현하였으며, 가장 높은 스코어를 가지는 화자를 인식화자로 결정한다. 화자인식에서 발성의 종류로는 문맥독립 문장을 사용하였다. 인식실험을 위해서는 ETRI445 DB와 KLE452 DB를 사용하였으며. 특징파라미터로서는 켑스트럼계수 및 회귀계수값만을 사용하였다. 인식실험에서는 등록화자의 수를 달리하여 일반적인 화자식별방법과 프레임단위유사도정규화방법으로 각각 인식실험을 하였다. 인식실험결과, 프레임단위유사도정규화방법이 인식화자수가 많아지는 경우에 일반적인 방법보다 향상된 인식률을 얻을수 있었다.

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Chemical Composition of Blue crabs Preserved in Soy Sauce (간장으로 염지한 꽃게장의 화학적 성분)

  • Lee, Fan-Zhu;Lee, Jin-Cheol;Jung, Dong-Sik;Yung, Ho-Chul;Eun, Jong-Bang
    • Korean Journal of Food Science and Technology
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    • v.33 no.6
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    • pp.714-719
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    • 2001
  • An interest in traditional foods is growing in an effort to preserve dietary culture in Korea. Blue crab preserved in soy sauce, one of the Korean traditional foods is especially popular in summer and is produced in the Jeollanam-do province, Korea. However, there has been no report on processing of the blue crab. The objective of this research is to investigate changes of nutritional composition in blue crab preserved in soy sauce. Moisture content was a little higher in FBC (Fresh Blue Crab) than in PBC (Preserved Blue Crab) and ash content was greatly higher in PBC than in FBC. Salt content was 1.50% in FBC and 7.89% in PBC. The amount of free sugars in FBC was very low but was increased after preserved using soy sauce, especially of fructose significantly. After preserved, the pH of blue crab was also increased. The contents of most total amino acids in blue crab were decreased after preserved, but free amino acids were increased. Major total amino acids were glutamic acid, arginine and aspartic acid in two samples and major free amino acids were arginine, proline and alanine. Fatty acid content was decreased after preserved and major fatty acids were palmitic acid (16 : 0), oleic acid (18 : l), eicosapentaenoic acid (20 : 5) and docosahexaenoic acid (22 : 6).

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Studies on the Farinograph Test and Crude Protein Content of Wheat Flour (밀가루의 조단백함량(粗蛋白含量)과 생지형성(生地形成)에 관(關)한 시험(試驗))

  • Kim, Hi-Kap
    • Korean Journal of Food Science and Technology
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    • v.6 no.2
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    • pp.61-64
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    • 1974
  • Six kinds of wheat flour were used for the assessments of the content of crude protein and the valorimeter value of Farinograph. Results obtained from these assessments are as follows. 1. In the case of common wheat varieties, a great differences are understood between Korean wheat and American wheat. The content of crude protein is negatively correlated to the valorimeter value in Korean wheat variefies, but there is a proportional tendency in American wheat varieties. 2. In the case of American wheat varieties, distinct differences are recognized between common wheat and durum wheat. The content of crude protein is directly correlated to the valorimeter value in common wheat varieties, but there is no proportional tendency in durum wheat.

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