Browse > Article

The Effect of Negative electric field using charged PTFE membrane on Bone Healing of Rabbit Long Bone  

Kwon, Yong-Su (Department of Periodontology, College of Dentistry, Kyungpook National University)
Park, Jin-Woo (Department of Periodontology, College of Dentistry, Kyungpook National University)
Lee, Jae-Mok (Department of Periodontology, College of Dentistry, Kyungpook National University)
Suh, Jo-Young (Department of Periodontology, College of Dentistry, Kyungpook National University)
Publication Information
Journal of Periodontal and Implant Science / v.34, no.3, 2004 , pp. 551-562 More about this Journal
Abstract
The purpose of this study was to evaluate the effects of negatively electric field on bone healing in rabbit segmental long bone defects using negatively charged PTFE membrane. Ten millimeter segmental defects in the rabbit radius were used as the experimental model. After membranes were then charge injected using a corona-charging apparatus, the left defects were covered with non charged PTFE membranes as control groups, whereas the right defect was covered with negatively charged PTFE membranes as test group. The animals were divided into 4 groups of 2 rabbits each, and sacrificed at 2, 4, 6, and 8 weeks. Histomorphometric analysis showed a more newly formed bone in negatively charged membrane at early healing period. At 2 weeks, the proportion of new bone formation to total defect area was 0.32% in control group, 1.10% in experimental group. At 4 weeks, the proportion of new bone formation to total defect area was 6.86% in control, and 13.75% in experimental. At 6 and 8 weeks, no obvious difference was found between the two groups but newly formed bone in test groups were slightly more than that in control groups. In conclusion, negatively charged membranes showed more newly bone tissue than noncharged membranes at an early healing period. Although the number of samples was small, this study showed that the combination of negatively electrical stimulation and P1FE membrane may be of value in long bone healing.
Keywords
Electric field; PTFE; Bone healing;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Seibert J, Nyman S. Localized ridge augmenta-tion in dogs: a pilot study using membranes and hydroxyapatite. J Periodontol 1990;61:157-163   DOI   PUBMED
2 Schmid J, Hammerle, CHF, Fluckiger L, Winkler JR, Olah AJ, Gogolewski S, Lang NP. Blood-filled spaces with and without filler materials in guided bone regeneration: A comparative exper-imental study in the rabbit using bioresorbable membranes. Clin Oral Impl Res 1997;8:75-81   DOI   ScienceOn
3 Terranova VP, Wikesjo UME. Extracellular matri-ces and polypeptide growth factors as mediantors of functions of cells of the periodon-tium: A riview, J Periodontol 1987;58:371-380
4 Bassett CAL, Pawluk RJ, Becker RO. Effects of electric currents on bone formation in vivo. Nature 1964;204:652   DOI   ScienceOn
5 Davidovitch Z, Shanfeld L, Montgomery PC. Biochemical mediators of the effects of mechani-cal forces and electric currents on mineralized tissues. Calcif Tissue Int 1984;36:S86-S97   DOI   PUBMED
6 Dmitriev AE, Mordrinov VI, Tatevosyan AS. Electric stimulation as a method of acceleration of healing of crural bone fractures. Vestn Khir Im II Grek 1976;116:73-75
7 Bassett CA, Pawluk RJ, Pilla AA. Augmentation of bone repairs by inductively coupled electro-magnetic fields. Science 1974;184:575-577   DOI   PUBMED   ScienceOn
8 Shigino T, Ochi M, Kagami H, Sakaguchi K, Nakade O. Application of capacitively coupled electric field enhances periimplant osteogenesis in the dog mandible. Int J Prosthodont 2000;13:365-372   PUBMED
9 Kubota K, Yoshimura N, Yokota M, Fizsimmons RJ, Wikesjo UME. Overview of effects of electri-cal stimulation on osteogenesis and alveolar bone. J Periodontol 1995;66:2-6   DOI   PUBMED   ScienceOn
10 Chierico A, Valentini R, Majzoub Z, Piattelli A, Scarano A, Okun L, Cordioli G. Electrically charged GTAM membranes stimulate osteogene-sis in rabbit calvarial defects. Clin Oral Impl Res 1999;10:415-424   DOI   ScienceOn
11 Bluhm AE, Laskin DM. The effect of polytetraflu-oroethylene cylinders on osteogenesis in rat fibular defects: a preliminary study. J Oral Maxillofac Surg 1995;53:163-166   DOI   ScienceOn
12 Nyman R, Magnusson M, Sennerby L, Nyman S, Lundgren D. Membrane-guided bone regenera-tion. Segmental radius defects studied in the rab-bit. Acta Orthop Scand 1995;66:169-173   DOI   PUBMED
13 Far O Nielsen F. Guided bone induction. A method in the treatment of diaphyseal long bone defect. Royal Dental College, Aarhus, Denmark 1992
14 Dahlin C, Sennerby L, Lekholm D, Linde A, Nyman S. Generation of new bone around tita-nium implats using a membrane technique: an experimental study in rabbits. Int J Oral Maxillofac Implants 1989;4: 19-25   PUBMED
15 Shandler HS, Weinstein S, Nathan LE. Facilitated healing of osseous lesions in the canine mandible after electrical stimulation. J Oral Maxillofac Surg 1979;37:787-792
16 Abeed RI, Naseer M, Abel EW. Capacitively cou-pled electrical stimulation treatment: results from patients with failed long bone fracture unions. J Orthop Trauma 1998;12:510-513   DOI   PUBMED
17 Schenk RK, Buser D, Hardwick WR, Dahlin C. Healing pattern of bone regeneration in mem-brane-protected defects: a histologic study in the canine mandible. Int J Oral Maxillofac Implants 1994;9:13-29   PUBMED
18 Wang Q, Zhong S, Ouyang J, Jiang L, Zhang ZQ, Xie Y, Luo S. Osteogenesis of electrically stimu-lated bone cells mediated in part by calcium ion. Clin Orthop 1998;348:259-268
19 Jahn TL. A possible mechanism for the effect of electrical potential on apatite formation in bone. Clin Orthop 1968;56:261-273
20 Brighton CT, Adler S, Blank J. Cathodic oxygen consumption and electrically induced osteogen-esis. Clin Orthop 1975;107:277-289   DOI   ScienceOn
21 Towfighi P, Arnold R. Use of a titanium-rein-forced membrane in periodontal regeneration: a case report. Compend Contin Educ Dent 1995;16:920-924   PUBMED
22 Fukada E, Yasuda I. On the piezoelectric effect of bone. J Physical Soc Jap 1957;12:1158-1162   DOI
23 Yasuda I. Fundamental aspects of fracture treatment. J Kyoto Med Soc 1953;4:395
24 Marino AA, Gross BD, Specian RD. Electrical stimulation of mandibular osteotomies in rab-bits. Oral Surg Oral Med Oral Pathol 1986;62:20-24   DOI   ScienceOn
25 Lundgren D, Lundgren AK, Sennerby L, Nyman S. Augmentation of intramembraneous bone beyond the skeletal envelope using an occlusive titanium barrier: An experimental study in the rabbit. Clin Oral Impl Res 1995;6:67-72   DOI   ScienceOn
26 Matsunaga S, Sakou T, Yoshikuni N. Intramedullary callus induced by weak direct current stimulation: Serial changes in the alkaline phosphatase activity at the site of electricity induced callus formation. J Japan Bioelect Res Soc 1988;2:67-71
27 Fizsimmons RJ, Farley JR, Adey WR, Baylink DJ. Frequency dependence of increased cell proliferation, in vitro, in exposures to a low-ampli-tude, low-frequency electric field: Evidance for dependence on increased mitogen activity released into culture medium. J Cell Physiol 1989;139:586-591   DOI
28 Wang Q, Xie Y, Zhong SZ, Zhang ZQ. The electrochemical reaction in tissue culture medium under direct current stimulation. Chin J Bioeng 1994;11:143-146
29 Fizsimmons RJ, Ryaby TJ, Magee FP, Baylink DJ. Combined magnetic fields increased net calcium flux in bone cells. Calcif Tissue Int 1994;55:376-380   DOI   ScienceOn
30 Ferrier J, Ross SM, Kanehisa J, Aubin JE. Osteoclasts and osteoblasts migrate in opposite kirections in response to a constant electric field. J Cell Physiol 1986;129:283-288   DOI   PUBMED
31 Hammerle CH, Karring T. Guided bone regener-ation at oral implant sites. Periodontal 2000 1998;17:151-175   DOI   ScienceOn
32 Togawa Y. Experimental study on the effect of direct currents on the internal remodeling of a long bone cortex. Nippon Skikeigeka Gakkai Zasshi 1983;57:817-835
33 Jovanovic SA, Schenk RK, Orsini M, Kenney EB. Supracrestal bone formation around dental implnts: an experimental dog study. Int J Oral Maxillofac Implants 1995;10:23-31   PUBMED
34 Inoue S, Ohashi S, Kajikawa. The effects of elec-tric stimulation on the differentiation to the bone. Orthop Res Sci 1980;7:501-507
35 Fizsimmons RJ, Strong D, Mohan S, Baylink DJ. Low-amplitude, low-frequency electric field-stimulated bone cell proliferation may in part be mediated by increased IGF-II release. J Cell Physiol 1992;150:84-89   DOI
36 Davidovitch Z, Korostoff E, FinkelsonMD. Effect of electric currents on gingival cyclic nucleotides in vivo. J Periodont Res 1980;15:355-362
37 Friedenberg ZB, Harlow MC, Brighton CT. Healing of non-union of the medial malleolus by means of direct current. A case report. J Trauma 1971;11:883
38 Heermeier K, Spanner M, Trager J, Gradinger R, Strauss PG, Kraus W, Schmidt J. Effects of extremely low frequency electromagnetic field (EMF) on cellagen type I mRNA expression and extracellular matrix synthesis of human osteoblastic cells. Bioelectromagnetics 1998;19:222-231   DOI   ScienceOn
39 Valentini RF, Vargo TG, Gardella JA, Aebischer P. Electrically charged polymeric substrates enhance nerve fiber outgrowth in vitro. Biomaterials 1992;13:183-190   DOI   ScienceOn
40 Weinstein AM, Kalwitter JJ, Cleveland TW, Amoss DC. Electrical stimulation of bone growth into porous $AL_{2}O_{3}$. J Biomed Mater Res 1976;10:231-247   DOI   ScienceOn
41 Brighton CT, Pollack SR. Treatment of nonunion of the tibia with a capacitively coupled electrical field. J Trauma 1984;24:153-155   DOI   PUBMED