• Korean Journal of Poultry Science
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• v.50 no.4
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• pp.303-310
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• 2023

The current study was conducted to compare the effect of crossbred on the body weight and laying performance of Korean native chicken from hatch to week 40. A total of 873 one-day-old chicks were divided into twelve crossbreds (i.e., CFCK, CFYC, CFYD, CKCF, CKYC, CKYD, YCYD, YCCF, YCCK, YDCF, YDCK, and YDYC) and commercial layer (Hy-Line Brown) were obtained as a counterpart in the study. All the birds are raised in battery cages (76 × 61 × 46 cm³) and then raised until 14 weeks and subsequently moved to layer battery cages (60 × 25 × 45 cm³) and raised until 16 weeks. The body weight and viability were measured biweekly from hatching to week 16. The week of 16, body weight range was about 1,010.24 to 1,411.77 g. The body weight of specific crossbreeds (i.e., CKCF, YCYD, and YDYC) was found to be comparable to that of Hy-Line Brown). The viability hatch to week 14 range was about 55 to 100% and however week 14 to 16 range was 80 to 100%. The crossbred (i.e., CKCF) recorded superior (P<0.05) viability throughout the whole experiment period, even compared with Hy-Line Brown (100% vs 96%). Our results are indicating that crossbreds Korean native chicken including CKCF, and YDYC has the potential to enhance key features of laying hens during the growing phase like optimal body weight and higher viability.

• Journal of the Korean Data and Information Science Society
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• v.26 no.3
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• pp.569-580
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• 2015

Log-rank is widely used for testing equality of two survival functions, and this method is efficient only under the proportional hazard assumption. However, crossing survival functions are common in practice. Therefore, many approaches have been suggested to test equality of them. This study considered several methods; Renyi type test, modified Kolmogorov-Smirnov and Cramer-von Mises test, and weighted Log-rank test, which can be applied when the survival functions cross, and simulated power of those methods. Based on the simulation results, we provide the useful information to choose a suitable approach in a given situation.

• Journal of Gastric Cancer
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• v.5 no.3 s.19
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• pp.174-179
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• 2005

Purpose: The 5-year survival rate is the most useful parameter for evaluating the effect of management on most malignant tumors. Recurrence after a curative resection for gastric cancer occurs mostly within 3 years of the operation, which caused us to evaluate whether a 3-year disease-free survival (3DFS) can be substituted for a 5-year overall survival (5OS). Materials and Methods: We reviewed the medical records of 656 consecutive patients who had undergone a curative resection for gastric cancer To assess whether 3DFS represents 5OS, we used a simple linear regression with survival probability calculated by using the survival function. Results: Recurrence was found in 175 cases during the follow-up periods. The accumulative frequencies of recurrence at postoperative 1 year, 3 years, and 5 years were 46% (81 cases), 89% (156 cases), and 97% (170 cases), respectively. The correlation coefficient (r) and the coefficient of determination $(r^2)$ between 3DFS and 5OS were 0.87 and 0.76, respectively, and the regression equation was $5OS=0.18+(0.80{\times}3DFS)$. The r and $R^2$ according to the type of recurrence were 0.89 and 0.80 in peritoneal seeding, 0.88 and 0.78 in hematogeneous metastasis, and 0.86 and 0.73 in local recurrence, respectively. The r (0.77) and $r^2$ (0.60) were relatively lower in low stages (stage I and II) compared to r (0.88) and $r^2(0.77)$ in high stages (stage III and IV). Conclusion: The 3DFS is an excellent predictor of 5OS. Therefore, if we use the former as the treatment evaluating method, 2-year time reduction in assessing and reporting treatment results is expected.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.203-206
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• 2000

Calcium carbonate $(CaCO_3)$ bead immobilized with alginate were developed as buffer system to enhance the cultivation efficiency of bifidobacteria. When Bifidobacteriuim longum KCTC 3128 and HLC 3742 were independently cultivated in 2.5-liter fermenter buffered the $CaCO_3$ bead, NaOH, $Na_2CO_3$, and $NH_4OH$. The proliferation of bifidobacteria and their storage stability were higher in culture broth buffered $CaCO_3$ beads than in culture broth buffered with NaOH, $Na_2CO_3$, and $NH_4OH$. Therefore, $CaCO_3$ bead may be useful as a buffer to enhance of the cultivation efficiency and viability of bifidobacteria.

• Environmental Analysis Health and Toxicology
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• v.20 no.1
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• pp.23-28
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• 2005

PAH 위해성 평가의 생체지표 개발을 위하여, benzo[a]pyrene을 인체 간암 세포주인 HepG2세포에 처리하여 암 억제 단백질인 p53 및 관련 단백질의 발현 양상에 대하여 연구하였다. HepG2 세포의 생존력은 benzo[a]pyrene을 노출시킨 군에서 농도가 증가할수록 감소하였다. p53과 인산화 p53의 발현 양상은 benzo[a]pyrene 농도 의존적으로 증가하는 경향을 보였으며, 반면에 아세틸화 p53은 benzo[a]pyrene의 농도가 증가할수록 감소하는 경향을 나타내었다. 세포 주기 조절에 관련된 p21 단백질은 화학 물질 처리에 의해서 p53과 마찬가지로 증가하였으나, CdK4와 Rb 단백질의 발현에는 변화가 없었다. 상관분석 결과 Benzo[a]pyrene 노출, 세포 생존력, p53, 인산화 p53, p21이 서로 높은 상관성을 보였다. 본 연구의 결과는 p53 단백질의 축적이 benzo[a]pyrene 독성에 있어 매우 중요한 현상이며, 이는 선택적인 지표와 함께 p53 이 benzo[a]pyrene과 같은 PAH 계열의 물질의 위해성 평가를 위한 민감한 생체 지표로써 개발될 수 있음을 시사한다.

• Food Science of Animal Resources
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• v.26 no.4
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• pp.503-510
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• 2006

Survival and stability of 19 bifidobacterial strains included 13 isolates from Korean infants against acid, bile acid and oxygen exposure were examined. Acid resistance of selected strains at pH values of 4.0, 3.0 and 2.0 was tested. Among the bifidobacterial strains tested, B. bifidum B3, B. longum D6, and B. adolescentis F1 exhibited higher viable cell counts exposed to acid whereas other strains had various results. The abilities of the strains to grow in the MRS broth containing 0.2% thioglycolic acid and 0.2% oxgall were tested and the tolerance of B. bifidum B3 and B. longum D6 to bile acid were higher than that of others. Even though in same species, the tolerance of tested strains to bile acid were variable. Stabilities of tested strains to oxygen exposure were variable and B. bifidum and B. longum strains showed relatively higher viable cell counts after 48 hours exposure to aerobic incubation. These results demonstrated that the survival and stability of bifidobacterial strains to acid, bile acid, and oxygen exposure were variable and strain-dependent. Due to their tolerant ability to environmental factors like acid, bile acid, and oxygen, B. bifidum B3 and B. longum D6 had good potential properties as probiotic cultures and may be useful for industrial application.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.129-129
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• 1993

현재 암의 치료에 이용되고 있는 항암제들은 therapeutic index가 낮아서 면역 및 조혈기능 장애 등 해결 되어야 할 문제점 들을 안고 있다. 이에 본 여구자는 우수하고 안전한 항암제를 개발하여 암의 치료에 이바지하고자, 화학연구소에서 in vitro 세로독성을 확인한 화합물에 대하여 in vivo 항암력을 실험, 치료효과가 높은 유도체/유사체 개발에 필요한 정보를 제공함에 본 연구의 목표를 두고 있다. 1차년도 연구에서는 화학연구소측이 제공한 KS 0409를 실험 약물로, sarcoma 180 복수암에 대한 in vivo 항암력을 실험하였다. 약 4주령의 SPE(specific pathogen-free) ICR계 마우스 및 BALB/c 마우스를 실험동물로 하여 sarcoma 180 암세포 부유액(세포 농도, 1$\times$$10^{7}$ cells/ml)을 실험동물의 복강내에 0.1ml 씩 이식하고 암이식 24시간 후부터 매일 1회씩 9회 약물 주사를 시행하였다. 대조약물 cisplatin은 2% DMSO-생리식염수를 주사하였다. 생존일수 관찰은 60dlfRK지 하였으며 % T/C를 계산하여 항암력을 평가하였다. 단 60일 생존 동물은 평균수명 계산에서 제외하였다.

• Proceedings of the Korean Society of Disaster Information Conference
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• 2017.11a
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• pp.271-272
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• 2017

자외선 에너지는 미생물의 DNA를 파괴함으로써 살균 효과를 일으킨다. 자외선 방사로 공기 중 미생물에 대하여 높은 살균력을 얻기 위해선 강한 자외선 강도와, 흐르는 공기가 충분한 시간 동안 강한 자외선에 노출되어야 하므로 균일한 자외선 방사는 매우 중요하다. 기존 UV살균 시스템은 강한 자외선 에너지와 공기의 빠른 이동에 필요한 UV방사의 균일성을 제공하지 않았다. 하지만 HDS의 BioProtector 제품에 사용된 Advanced UV System(AUVS) 반사 캐비티 기술은 매우 높은 수준의 균일성을 생성함으로써 이 문제에 대한 해결책을 제공하며, 미생물 살균에 가장 효과적인 UV파장을 사용하여 6 LOG KILL (100만마리의 미생물 투입시 1마리 이하생존) 수준의 살균 효과를 만들었다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.3
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• pp.247-257
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• 2014

Oil-in-water nanoemulsions of astaxanthin prepared by new vesicle, glyceryl citrate/ lactate/ linoleate/ oleate, were evaluated thoroughly in terms of cosmeceutical properties such as antioxidant effect, cell viability, influence of protein related enzyme, skin penetration, skin hydration and elasticity. Antioxidant effect and cell viability of nanoemulsion of astaxanthin were evaluated by DPPH and MTT assay. Also other properties of nanoemulsions of astaxanthin were measured by proteome analysis using 2D-PAGE, confocal laser scanning microscope and in-vivo test. We were able to find that the nanoemulsion of astaxanthin is good at scavenging of radical and inhibits the degradation of dermal extracellular matrix with the down-regulation of MMPs and other proteins related to MMP expression. CLSM was adopted for observing penetration of nanoemulsion of astaxanthin and showed high effective penetration rate compared to the nanoemulsion of astaxanthin prepared by conventional lecithin. In-vivo measurement of the nanoemulsions in hydration and elasticity were conducted to 11 Korean female adults for 28 days and showed better results.

• Korean Journal of Animal Reproduction
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• v.25 no.2
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• pp.191-198
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• 2001

The purpose of this study was to investigate the effects of embryo sources such as in vivo vs. in vitro produced blastocyst, and culture systems on the membrane permeability and viability of bovine blastocyst following GMP vitrification. To produce in vivo embryos, six cows were superovulated by administration of follicle stimulation hormone (FSH) and prostaglandin $F_{2{\alpha}}$(PG $F_{2{\alpha}}$). in vitro embryos were produced by two different culture systems, oviduct co-culture (OCS) and defined culture system (HECM-6; DCS). Ovaries were picked up at a local slaughterhouse and transported to laboratory in 3$0^{\circ}C$ saline within 2 h. Ovaries were washed with same saline three times and then placed in saline on warm plate adjusted at 3$0^{\circ}C$ during aspiration. The blastocysts produced were assigned for membrane permeability and viability following GMP vitrification. The membrane permeability of blastocysts was checked in 0.5 M sucrose solution on warm plate at 35$^{\circ}C$ for 0, 2, 5 and 7 min, respectively. Then the diameters (width and length) of embryo cytoplasms were measured by a eyepiece meter, and they were converted to their volume by 4/3 $\pi$ $r^3$. The blastocysts were cryopreserved by GMP vitrification method, where they were sequentially placed into vitrification solution before being loaded into GMP vessels and immersed into L$N_2$ within 20 to 25 sec. Post-thaw blastocysts were serially washed in 0.25 and 0.15 M sucrose in HM and TCM-199 for 5 min each, and then cultured in TCM 199 supplemented with 10% FCS for 24 or 48 h. The volume change of in vivo blastocyst at 0, 2, 5 and 7 min (100, 37.1, 34.3 and 31.6%) was significantly more shrunk than those of in vitro blastocysts derived from OCS (100, 59.8, 48.9, 47.9%) and DCS (100, 57.2, 47.3 and 46.9%) (P＜0.05). The viability of post-thaw blastocyst derived from in vivo (93.6%) was also significantly different from those in OCS and DCS (81.9 and 83.6%; P＜0.05). In the present culture system, the morphology of embryos produced in vitro was similar to that of in vivo embryos, but the quality in membrane permeability and post-thaw viability showed a big difference from their sources as in vivo or in vitro derived from OCS and DCS. The results indicated that the quality of in vivo embryos in membrane permeability and post-thaw viability was better than those of in vitro embryos derived from OCS or DCS.