• Korean Journal of Microbiology
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• v.40 no.3
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• pp.232-236
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• 2004

We investigated hydrogen peroxide resistance of Escherichia coli possessing acetyl xylan esterase(AxeA) of Streptomyces coelicolor A3(2). The induction of AxeA production by isopropyl-$\beta$-thiogalactoside was confirmed by SDS-polyacrylamide gel electrophoresis. The differences in growth between induced and non-induced E. coli were determined by the changes in optical density of cultures after hydrogen peroxide treatment The lethal effect of hydrogen peroxide was observed for non-induced cultures at all concentrations tested in this study (lmM, 2.5mM and 5mM). However, cultures induced for AxeA production resisted the lethal effect, except at 5mM where cells were killed irrespective of the AxeA production. The axeA induction increased survival against 1.5mM hydrogen peroxide from 59% to 74%. In addition, AxeA producing E. coli showed increased survival at $45^{\circ}C$, near maximum growth temperature. Therefore, it was concluded that AxeA conferred a cross-resistance upon the bacterium against both oxidative- and heat stress.

• Korean Journal of Plant Resources
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• v.10 no.4
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• pp.375-381
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• 1997

This study was conducted to determine the effect of seedling and division nursery stock size on the growth and yield of Wasabia japonica cultivated with cold water from Soyang Dam. As seedling and division nursery stock size were enlarged, their growth and yield were higher. In the case of division nursery stock, the rhizome yield per 10a were 782kg for large one, 723kg form medium one and 567kg for small one, and commercial rates - the percentage of rhizomes that weigh over 40g - were 87% for large one,80% for medium one and 55% for small one. In the case of seedling, large one brought out yield of 575kg/10a while medium and small one brought out low yield of 408kg/10a, and 421kg/10a, respectively. And the commercial rates from seedling were 60% for large one. 13% for medium one and 20% for small one.

• Horticultural Science & Technology
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• v.33 no.6
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• pp.829-838
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• 2015

Garlic (Allium sativum L.), one of the oldest cultivated crops, is the most widely used Allium species belonging to the family Lilliaceae. In this study, growth characteristics, photosystem II activity, and antioxidative enzyme activity were investigated in five temperatures ($10-30^{\circ}C$) during early growth stage of garlic to determine the optimum temperature for cultivation and assess the effects of high temperature on early growth of garlic. Vegetative growth (e.g., shoot height, number of leaves) of garlic plants was greater in the temperature ranges of $15-25^{\circ}C$. However, dry weight (of shoot, bulb, and total plant) of garlic was significantly greater at $20^{\circ}C$, compared to either below or above $20^{\circ}C$. $F_v/F_o$ and $F_v/F_m$ values were highest at $15-20^{\circ}C$, and decreased above $25^{\circ}C$. The chlorophyll a fluorescence induction OKJIP transient was also considerably affected by high temperature; the fluorescence yields $F_i$ and $F_P$ decreased considerably above $25^{\circ}C$, with the increase of $F_k$ and $W_k$. Activities of catalase and superoxide dismutase in leaves and peroxidase in roots were high in $20-25^{\circ}C$, and decreased significantly in $30^{\circ}C$. These results indicate that a growth temperature of $30^{\circ}C$ inhibits early growth of garlic and that it is desirable to culture garlic plants near $20^{\circ}C$. Fluorescence parameters such a $F_v/F_o$, $F_v/F_m$, $F_k$, $ET_o/CS_m$, and $PI_{abs}$ were significantly correlated with dry weight of whole garlic plants (p < 0.01), indicating that these fluorescence parameters can be used for early assessment of high temperature effects even though the damage to the plant is not very severe.

• Korean Journal of Microbiology
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• v.52 no.4
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• pp.406-414
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• 2016

Among 6 leu codons, CUG is the most frequently used codon in E. coli. It is recognized by leu-tRNA(CAG) encoded by four genes scattered on two chromosomal loci (leuT and leuPQV ). In the process of constructing a strain with no functional leu-tRNA (CAG) gene on chromosome, we made two mutant strains separately, one on leuPQV locus (${\Delta}leuPQV$), and the other on leuT locus [$leuT^*$(GAG)], where the anticodon of leuT was changed from CAG to GAG, thereby altering its recognition codon from CUG to CUC. We attempted to combine these two mutations by transduction using $leuT^*$(GAG) strain as a donor and ${\Delta}leuPQV$ strain as a recipient. Large and small colonies appeared from this transduction. From PCR and DNA sequencing, large colony was confirmed to be the reciprocal recombinant as expected, but the small colonies contained both mutant $leuT^*$(GAG) and wild type leuT (CAG) genes in the cell. This heterozygous diploid strain did not show any unusual morphology under microscopic observation, but, interestingly, it showed a linear growth curve in rich medium with much slower growth rate than wild type cell. It always formed homogenous small colonies in the selection medium, but, when there was no selection, it readily segregated into $leuT^*$(GAG) and leuT (CAG). From these observations, we suggested that the strain with both $leuT^*$(GAG) and leuT (CAG) genes was not a partial diploid (merodiploid), but a full diploid cell having two different chromosomes. We proposed a model explaining how such a heterozygous diploid cell was formed and how and why its growth showed a linear growth curve.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.226-233
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• 2005

This study was carried out to evaluate the fruit and growth characteristics of selecting Vitis amurensis through functional material analysis and sensory evaluation in V. amurensis collected in Gangwondo. For evaluation of growth characteristics in V. amurensis, experiments were carried out by compared with the two grape cultivars 'Campbell Early' and 'Kyoho'. The full bloom and verasion time in V. amurensis was investigated faster than those of examined cultivar grapes, while harvest time was investigated latter than those of examined cultivar grapes, but agronomic characteristics was not thought significantly difference between cultivar grapes and V. amurensis. For evaluation of shoot growth phase, the growth curve was very similar to cultivar grapes. The berry size in V. amurensis showed that increases rapidly between 3 and 4 days after full bloom time, and approximately doubles between the second growth period and harvest time, and the berry development phase investigated that consist of two sigmoid growth periods separated by a lag phase. The berry weight and soluble solids in V. amurensis increased with the tree age, but acidity and total sugar contents decreased, and showed a special quality and stable growth according to vine age. To investigation of functional materials, the anthocyanin content in V. amurensis ranged from 16.6 to 50.2 mg/100 g, and the resveratrol content ranged from 0.143 to $0.236{\mu}g/100\;g$ which was higher than those of cultivar grapes. These result indicated that V. amurensis tended to have the useful material larger than cultivar grapes. Therefore, other edibility factors of V. amurensis collected in Gangwondo may contribute to breeding studies in Vitis. spp.

• Korean Journal of Medicinal Crop Science
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• v.3 no.2
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• pp.77-80
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• 1995

This study was carried out to know the effect of air temperature on photosynthesis and growth of Korean Valerian (valeriana fauriei var. dasycarpa HARA). The results are as follows; 1. Photosynthesis of V fauriei had highly significant relation to temperature in a quadratic regression mo­del, from which the optimum temperature for the plant growth were estimated to be $17.7^{\circ}C$. During the day­time in the field condition, the photosynthesis rate was highest at 9 a.m, then reduced to minimum at 2 p.m., and increased again thereafter. 2. Number of stomata was about $25/mm^2$ on the upper side and $85/mm^2$ on the lower side of the leaf, and the size was $21{\sim}30/{\mu}m$ in diameter. 3. A highly significant quadratic regression was noted between temperature and leaf width or root weight of V. fauriei. It was estimated from the regression equation that the optimum temperature for root growth was $20.3^{\circ}C$.

• Korean Journal of Medicinal Crop Science
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• v.4 no.1
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• pp.7-11
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• 1996

This study was conducted to know the effects of light intensity and temperature on growth of V. fauriei plants. Photosynthesis of V. fauriei had highly significant relations to light intensity and temperature in a quadratic regression model, from which the optimum light intensity and temperature for the plant growth were estimated to be 40,000lux and $17.7^{\circ}C.$ Root was produced less by shading at Jinbu where is located in alpine region, but root yield is increased by shading at Umsong where is located in plane region. Roots were produced more in Jinbu than in Umsong. A highly significant quadratic regression was noted between temperature and leaf width or root weight of V. fauriei. It was estimated from the regression equation that the optimum temperature for root growth was $20.3^{\circ}C.$

• Journal of Bio-Environment Control
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• v.17 no.3
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• pp.238-245
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• 2008

This study was carried out to investigate the effect of magnesium (Mg) concentrations in nutrient solution on growth of and nutrient uptake by 'Maehyang' strawberry. Tissue analysis based on dry weight and petiole sap were also conducted to determine the threshold level in plants when Mg deficiency disorders developed in strawberry plants. In the Mg deficient plants, the spotted yellowing or yellowing area developed on the interveinal area of mature leaves. The dark brown color also developed on the interveinal area of mature leaves with marginal browning or marginal necrosis. The response in dry weight production of 'Maehyang' strawberry to elevated Mg concentrations in nutrient solution was quadratic and the equation is y=6.84+1.7533x-$0.9278x^2$ $(R^2=0.1081^{***})$. But the Mg contents in tissue increased lineally with the equation of y=0.1764+0.1275x $(R^2=0.8307^{***})$. The trends of fresh weight production and Mg concentrations in petiole sap were also quadratic (y=24.127+7.3565x-$1.125x^2$, $R^2=0.2314^{^***}$) and linear (11.954+5.793x, $R^2=0.6869^{***}$), respectively. To prevent growth suppression, the Mg concentrations based on dry weight of above ground tissue and in petiole sap should be in the range of 0.30 to 0.65% and 19 to $40mg{\cdot}kg^{-1}$, respectively, fer the commercial production of 'Maehyang' strawberry.

• Journal of Bio-Environment Control
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• v.24 no.2
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• pp.106-112
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• 2015

The objective of this study was to examine the growth reaction of strawberry plants to the mixed red and blue LED sources and their installation method. The artificial light sources were : LED PAR(PPFD $2{\sim}4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), LED BAR(PPFD $100{\sim}120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and incandescent(PPFD $2{\sim}4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) lamp. The lighting treatment was started at the first cluster flowering period as a night breaking lighting and was applied during 3 hours, between 22:00 and 01:00 every day. Plant height and leafstalk length were longer in plants treated with incandescent lamp, where as fresh and dry weight of shoot were heavier in LED PAR compared to incandescent lamp treatment. LED PAR treatment also resulted in the largest leaf area, chlorophyll content was increased by $0.36mg{\cdot}g^{-1}$ after 60 days from the starting of the artificial lighting. According to the experimental results application of 16W LED PAR lamps and W-type installation method can improve light environment in strawberry lighting culture.

• Korean Journal of Medicinal Crop Science
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• v.8 no.4
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• pp.362-372
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• 2000

This study was conducted to obtain the basic information on the production of high yield and qualitied yam in Andong, major yam-producing district, in Kyongbuk province, using three major Yam cultivars, such as Danma, Jangma and Alata (round type). Alata showed the longest tuber width while Jangma showed the longest tube length. Danma showed the highest tube yield at 2,123 kg/10a. Tubes of Alata had higher dry ratio than those of other cultivars, suggesting that Alata could be used as good processing materials. Characteristics of top parts, such as vine length, leaf number, and lateral vine number showed typical sigmoid curves. Tuber characteristics such as length, width and fresh weight of tuber were linearly increased as the growth period was progressed. Relative growth rate (RGR) for new tuber and stem reached the highest value at 86 DAT (days after transplanting). There were similar tendencies among the cultivars in net assimilation rate (NAR). The highest crop growth rate (CGR) was appeared at 156 DAT regardless of cultivars. In the changes of leaf area ratio (LAR), there were significant differences among three cultivars. LAR were decreased at 86, 106 and 136 DAT for Alata, Danma and Jangma, respectively. However, specific leaf weight (SLW) was greatly increased at 176 DAT, and leaf area index (LAI) was decreased at 156 DAT in all cultivars.