• Korean Journal of Environmental Agriculture
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• v.21 no.4
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• pp.261-268
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• 2002

Biodegradation of monochlorophenols by wood rot fungi such as Daldina concentrica, Trametes versicolor and Pleurotus ostreatus was evaluated by determining their resistance or toxic test and biodegradability. The metabolites of monochlorophenols were also analyzed. Among the three fungi, T. versicolor was the most resistant to 200 ppm of 2-, 3- and 4-chlorophenols, and did not show any inhibitory mycellium growth. But D. concentrica had a little inhibition effect at more than 100 ppm of 3- or 4-chlorophenol. Control cultures of P. ostreatus took even 14 days far the completion of mycellium growth, but the hyphal growth was improved when 2- or 3-chlorophenol were added to the culture. In biodegradation analysis, P. ostreatus showed the highest degradation of 2- or 3-chlorophenol, while T. versicolor was the most effective in 4-chlorophenol. D. concentrica and P. ostreatus slowly degraded 4-chlorophenol. However, T. versicolor had similar degradation capability in the three monochlorophenols, suggesting that the biode- gradation nude is dependent on the fungi as well as the type of monochlorophenol. Several metabolites such as 1,3,5-trihydroxyl benzene, 1-ethyl-1-hydroxyl pentane, 2-propenoicacid, methylmalonic acid and 2-methyl-4-keto-pentan-2-ol were found as products of primary oxidation of 2-, 3- and 4-chlorophenols by intact fungal cultures. fatty acids including tetradecanoic, heptadecanoic and octadecanoic acids were also detected The order of increase of mycellium weight during incubation were P. ostreatus > T. versicolor > D. concentrica. The pH in the culture was not constantly changed depending on incubation days, but the mycellium weight was slightly increased, indicating that the biodegradation of monochlorophenol might have low relationship with the mycellium growth Laccase activities of T. versicolor and P. ostreatus were continuously increased depending on the incubation days, suggesting that the ligninolytic enzyme activity play an important role in the biodegradation of monochlorophenol.

• Applied Biological Chemistry
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• v.8
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• pp.87-93
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• 1967

1) For the micro-analysis of mercury in plant materials, the method of Furutani was shown to be the simplest and most efficient way and the recovery of the assay was about 98%. 2) When the rice grain was soaked in 1/1000 diluted solution of organo-mercury fungicide for 8 hours at the end of March, the amounts of mercury residues in the brown rice and unhulled rice were 8.8 to $9.5\;{\mu}g/g$ seeds and 10.1 to $10.7\;{\mu}g/g$ seeds, respectively. 3) By washing the treated rice seeds with running water for three days, tile residual mercury concentration was reduced to 1/4 to 1/5; thus the mercury residues were 1.86 to $1.92\;{\mu}g/g$ for brown rice and 1.96 to $2.93\;{\mu}g/g$ for unhulled rice. 4) The residual mercury was present more in the unhulled rice than in the brown rice, either before or after washing of the treated seeds. 5) Among the different rice varieties, no difference was observed in mercury residues by seed treatment and washing.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.944-947
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• 2007

Bacillus sp.LPO3 (producing emulsifying substances such as bio-surfactant) was used as a bio-control agent to degrade hydrocarbon (gasoline in oil spilled crop soil). The soil (brought from fertilizer store)was mixed with gasoline-spilled soil (made with Diatomaceous Earth, Sigma.U.S.A). The study was conducted for a period of 13 days, 13 days during which bacterial growth, hydrocarbon degradation and growth parameters of Bacillus sp.LP03 including shoot and root length were studied. We found that the effective of bacterial producing substance might bio-surfactants let the plants survive even more promote the growth of shoot and root length and showed antifungal activity against gray mold. Without the bacteria, they couldn't grow in oil-spilled soil not even survive. According to the results of the above experiments, we can see with following results, hydrocarbon in gasoline was reduced, day by day, then RNA dot blotting was done and it fit the results we had done. Finally, this Bacteria(producing bio-surfactant) were found to have effective bio-control agent for cropping in oil spilled soil and infected by gray mold.

• Horticultural Science & Technology
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• v.31 no.2
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• pp.135-140
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• 2013

This experiment was conducted to find the effects of a $GA_3$ and thidiazuron (TDZ) on seedless rate, harvest time, fruit cracking and fruit quality in 'Kyoho' grapes over two years from 2008 to 2009. In 2008, fruit clusters were dip treated with $GA_3$ $25.0mg{\cdot}L^{-1}$ twice at full bloom (FB) and 14 days after full bloom (DAFB) in a combination with TDZ 0 or $2.5mg{\cdot}L^{-1}$. Berry seedless rate and berry enlargement were slightly improved only when TDZ was added to the second $GA_3$ treatment at 14 DAFB, compared to $GA_3$ + TDZ treatments at both FB and 14 DAFB. However, berry cracking rate was significantly increased by any plant growth regulator (PGR) treatments compared to non treatment. In 2009, $GA_3$ at $12.5mg{\cdot}L^{-1}$ and $25.0mg{\cdot}L^{-1}$ was dip treated twice at FB and 14 DAFB while TDZ $2.5mg{\cdot}L^{-1}$ was treated only at 14 DAFB. Berry cracking rate was depended on the concentration of $GA_3$ applied. The higher concentration at $25.0mg{\cdot}L^{-1}$ significantly increased berry cracking rate while the lower concentration at $12.5mg{\cdot}L^{-1}$ had no effect. Also, the addition of TDZ to $GA_3$ $25.0mg{\cdot}L^{-1}$ at 14 DAFB, substantially decreased the cracking rate to the level of untreated control. Although all PGR treatments advanced fruit maturity, the most significant advance occurred when TDZ was added to $GA_3$ $12.5mg{\cdot}L^{-1}$ only at the second dip. Considering the overall aspects related to fruit maturity and quality, we concluded that the double applications of $12.5mg{\cdot}L^{-1}$ $GA_3$ at FB and 14 DAFB with addition of $2.5mg{\cdot}L^{-1}$ TDZ only at 14 DAFB was appropriate to produce about 400-500 g size of seedless 'Kyoho' grape cluster having 35-40 berries.

• Korean Journal of Medicinal Crop Science
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• v.4 no.2
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• pp.172-177
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• 1996

The influences of testa-removal of seed, partial cutting of embryo, xylene treatment, growth regulator, chemical treatment and low temperature treatment on the seed germination of Lycoris aurea were investigated. The germination rate of the testa-removed seeds was higher than that of the whole seeds, and the seeds treated by testa-removal+xylene was 63% of germination. $GA_3$ was more effective than kinetin treatment, and seeds treated with $GA_3$ 200ppm showed 60% of germination. $KNO_3$ was more effective than KOH treatment, and seeds treated with 0. 1% $KNO_3$ was 76% of germination. The seeds, stored for two months at $5^{\circ}C$, showed the highest germination rate with 93%

• KOREAN JOURNAL OF CROP SCIENCE
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• v.14
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• pp.139-145
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• 1973

Effects of Choline Chloride on growth and yields of sweet-potato cultivated with the single crop and after-barley crop were summarized as follows. 1. The greatest effect was obtained when the sprout-bases of sweet potato were soaked in the solution of Choline Chloride for 24 hours, while the optimum concentration of Choline Chloride was 32.3ppm in case of single crop and 31.1ppm in after barley crop respectively. 2. Choline Chloride restrained the growth of stem; the length shortened and the dry weight decreased. 3. The number of tubers and yields were increased by treating choline chloride. It may be thought that the translocations of assimilation substance from leaves and stem to tubers, was stimulated by treating choline chloride. The tendancy of higher yielding was shown in the early harvesting than in the usual harvesting 4. Sugar and starch content were higher, crude fiber and crude protein content were lower as compared with the control.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.2
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• pp.141-146
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• 1982

In the vine training methods, main vine training yield was 36% higher than the non-training. Main vine training date was studied for increasing cone yield in hops. Training was done four different dates from May 14 to May 29 including non-training as a check. Earlier training increased cone yield from 12 to 43% due to more beared branches per plant, longer branch length caused by accelerating the branch growth, and an increased of number of cone per beared branch and total cone number.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.54-54
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• 2020

본 연구에서는 제방의 세굴이나 붕괴를 방지하기 위해 바이오폴리머(Biopolymer) 기반 신소재를 활용한 흙 제방의 보강공법을 제시하였다. 바이오폴리머 기반 제방의 보강공법은 흙과 바이오폴리머를 소량만 섞어도 흙의 강도 증진시킴과 동시에 빗물에 대한 내침식성과 식생의 생장을 촉진하는 생태성도 뛰어나기 때문에 제방 사면을 보호할 수 있는 친환경적이고 효율적인 공법이다. 이에 안동하천연구센터는 실증실험을 통한 신소재 제방 보강공법의 안정성 검증을 목표로 2 건의 월류붕괴 실험을 수행하였다. 첫 번째는 흙 제방 조건(Case 1)이며, 두 번째는 바이오폴리머 혼합 토양을 사면에 도포한 후 식생이 활착된 조건(Case 2)이다. 제방 붕괴에 따른 수로 내 수위변화를 측정하기 위해 압력식 수위계를 설치하였으며, 영상분석을 위한 다수의 카메라 및 드론을 활용하여 실험의 전 과정을 실시간 촬영하였다. 또한, 제내지 측 사면을 대상으로 월류에 따른 붕괴 지연효과를 정량적으로 제시하기 위해 이미지 픽셀 변화 측정 기법을 통한 시간에 따른 표면 손실률을 산정하였다. 흙 제방과 신소재 처리 제방의 시간에 따른 표면손실률을 비교한 결과, Case 2의 사면손실률이 Case 1에 비해 약 1.5~2.3 배 지연되는 것을 확인하였다. 하지만 단일 조건만으로 실험군과 비교군의 붕괴지연 결과가 제방 성능을 평가함에 있어서 일반화될 수 없으므로 이러한 정량적 평가는 다소 한계가 있다. 향후 이러한 부족한 부분을 해결하기 위한 노력과 다양한 조건의 추가실험을 통한 계측 데이터 및 붕괴지연시간의 평균값을 도출하여 신소재 제방의 안정성을 평가하기 위한 타당한 결과를 도출할 예정이다.

• Korean Journal of Plant Tissue Culture
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• v.24 no.2
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• pp.119-124
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• 1997

Plantlets were regenerated from various explants (shoot tip, leaf blade, petiole and root segments) via organogenesis and/or somatic embryogenesis from Armoracia rusticana(Lam) Gaerth., Mey, et Scherb.. Shoot regeneration rate from callus was highest on the MS mediums supplemented with 0.5 ㎎/L IAA, 5.0㎎/L BA and 10.0㎎/L spermine. A Low frequency of regeneration occurred on hormone-free MS medium. Multiple shooks were regenerated at a pH of 4.0 to 8.0 on MS medium supplemented with 1.0 ㎎/L BA and 0.1 ㎎/L NAA. Polyamines promoted shoot- and root-formation by 2 to 4 times normal, Specific proteins associated with organogenesis were identified. Somatic embryogenesis occurred directly from the leaf blade, petiole and root segments cultured on MS medium with 2.0 ㎎/L BA and 2.0 ㎎/L BA and 2.0 ㎎/L NAA. Three types of regeneration in A, rusticana were clearly established, which could be applied to the study of morphogenesis and genetics at cell, tissue and organ levels.

• Journal of Bio-Environment Control
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• v.6 no.3
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• pp.151-158
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• 1997

Tomato (lycopersicon esculentum Mill.‘Sunroad’) seedlings grafted onto the four different rootstocks, ‘Anchor-T’, ‘Kagemushia’, ‘Joint’ and ‘Vulcan’, and fed with different amount of nitrogen were investigated for their susceptibility to the root rot Fusarium wilt (Fusarium oxysporum Schl. f. sp. lycopersici Snyder et Hansen, race J$_3$) disease, plant growth, and fruit quality. The flowering of the first flower cluster was promoted in grafted plants (GP) as compared to non-grafted (NG) ones. The plant height was increased by the grafting, and the growth and stem diameter were enhanced with increased nitrogen levels. Grafted plants were little affected by the Fusarium wilt disease except ‘Anchor-T’rootstock (64.7％ infection). The rate of malformed, underdeveloped, and gray-mold infected fruits were reduced in the grafted plants regardless of the rootstocks. Soluble solid content, sugars, ascorbic acids and organic acids did not show any difference between grafted and non grafted plants, but ascorbic acid tends to be reduced in the high nitrogen treatment. Content of N, Ca and Mg in leaves were greatly increased in the GP grown with high nitrogen level (30kg.㏊$^{-1}$ ).