• Korean Journal of Environment and Ecology
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• v.24 no.6
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• pp.648-656
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• 2010

Global warming brings changes of natural ecosystems and affects on the plant growth response. Quercus acutissima and Q. variabilis are taxonomically similar and dominant native species in deciduous forests in South Korea. In order to understand the growth response of Q. acutissima and Q. variabilis to global warming condition, we cultivated the seedling of the two oak species in ambient condition(control) and treatment with elevated $CO_2$(700~800ppm) and increased air temperature(approximately $3^{\circ}C$ above than control). Then we measured the growth characteristic among them and analyzed the relationship between two species using PCA ordination. Stem length and total plant weight of Q. acutissima were significantly affected by elevated $CO_2$ concentration and increased air temperature. Stem diameter and weight of Q. variabilis were significantly affected by elevated $CO_2$ concentration and increased air temperature(p<0.05). The variation characteristics of Q. acutissima were changed more than Q. variabilis by elevated $CO_2$ concentration and increased air temperature. These result suggested that Q. acutissima was more sensitive to global warming situation than Q. variabilis in central region of Korea. PCA ordination showed that two species were arranged by two distinct groups based on 10 characters by elevated $CO_2$ and increased air temperature.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.2
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• pp.125-136
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• 1981

This experiment was conducted to study the influence of different cultivation periods on some morphological and agronomic characteristics of rice plant at Iri, the Honam plain, in 1979. Early maturing varieties showed little delay in heading with late transplanting, but intermediate and late maturing varieties showed more than 7 day delay in heading. The number of days required to reach heading was over 55 and 67 days after transplanting in early and intermediate-late maturing varieties, respectively. There was significantly postitive correlation between the length of vegetative stage and the grain yield. The mean temperature during ripening period was significantly correlated with the grain yield and the percentage of filled grains. The length, width, and thickness of leaf increased with earlier transplanting and the elongation degree between upper and lower leaves showed the same tendency. The productivity of dry matter and the translocation rate of photosynthate to spikelet decreased with late transplanting mainly due to low temperature. LAI was also influenced by transplanting date showing close correlation with yield. Early transplanting was desirable for assimilation and dry matter accumulation.

• Journal of Korean Society of Forest Science
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• v.84 no.1
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• pp.77-86
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• 1995

To effectively modify tree function with genetic engineering, transgenes must be expressed at the proper level in the appropriate tissues at suitable developmental stages. Toward understanding the spatial and temporal expression of transgenes in woody plants, transgene expression was evaluated in three greenhouse-grown, transgenic lines of Populus alba ${\times}$ P. grandidentata hybrid clone 'Hansen'. All transgenic poplar lines possess constructs containing the bacterial nopaline synthase(nos) promoter linked to a neomycin phosphotransferase II(NPT II) selectable marker gene. In addition, each transgenic poplar line contains one of the following gene constructs : 1) a wound-inducible potato proteinase inhibitor II (pin2) promoter linked to a chloramphenicol acetyltransferase(CAT) reporter gene. 2) a nos promoter linked to a PIN2 structural gene : or 3) a Cauliflower Mosaic Virus 35s promoter linked to a PIN2 structural gene. Polymerase chain reaction(PCR) was used to verify the presence of foreign genes in the poplar genome. Enzyme-linked immunosorbent assays(ELISAs) were used to evaluate organ specific expression of the nos-NPT II construct. NPT II expression was detected in leaves, petioles, stems, and roots of transgenic poplar, thereby indicating that the nos promoter is potentially effective for general constitutive expression of transgenes. NPT expression varied among transgenic poplar lines and among organs for one transgenic line, Tr15. With Tr15, NPT II levels were highest in older leaves and petioles. These results indicate that screening of several transgenic lines may be required to identify lines with optimal transgene expression.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.2
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• pp.216-226
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• 1983

Genetic studies on the $F_2$ generation of a set of half diallel crosses involving six cowpea varieties were conducted. by the randomized block design with three replications to determine combining ability, gene action and the relationships between parents and their $F_2$ hybrids. The 12 agronomic characters namely, days to flowering, days from flowering to maturity, days to maturity, diameter of stem, length of internode, number of branches per plant, length of pod, number of pods per plant, number of grains per pod, number of grains per plant, 100 grain weight and grain weight per plot were observed, and the $F_2$ generation of this diallel set of crosses was analysed for each character according to the method by Jinks and Hayman. The results obtained are summarized as follows: 1. Vr-Wr graphical analyses; The following seven characters, days to flowering, number of branches per plant, length of pod, number of pods per plant, number of grains per plant, 100 grain weight and grain weight per plot appeared to be partially dominant, and over dominance was found for days from flowering to maturity, days to maturity, length of internode and number of grains per pod. But diameter of stem indicated partial dominance near complete dominance. 2. Estimates of genetic variance components; In the degree of dominance,. eight characters, that is, days to flowering, days from flowering to maturity, days to maturity, length of internode, number of pods per plant, number of grains per pod, number of grains per plant and grain weight per plot were expressed larger than 1. And the characters, days from flowering to maturity, number of branches per plant and number of grains per plant as the degree of mean dominance ($H_1$/D) were found to be negative value over other characters. On the other hand, apprent asymmetry of dominance-recessive allele ($H_2$ /$4H_1$) produced comparatively estimates with lower value on days from flowering to maturity, length of internode, number of branches per plant and number of grains per pod. 3. Analyses of combining ability; Mean square value of GCA(general combining ability) appeared to be more important than those of SCA (specific combining ability) for most characters, and among them, grain weight per plot showed the highest mean square value in GCA and SCA. 4. Effect of combining ability; Variety 178 was expressed as the highest GCA effects in five characters (days to flowering days to maturity, number of pods per plant, number of grains per plant and grain weight per plot). SCA effects were differed from parents, characters and crosses, but crosses between TVu 1857 $\times$ TVu 2885 and TVu 2702 $\times$ J78 were shown to be highly with SCA effects on yield.

• Applied Biological Chemistry
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• v.40 no.3
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• pp.173-177
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• 1997

Bacillus licheniformis 9945a releases a natural ${\gamma}-poly(glutamic\;acid)({\gamma}-PGA)$ into fermentation broth and shows a mucoid phenotype on the solid agar medium. Transformation of mucoid cells of Bacillus species has not been simple and straightforward. The transpositional activity of Tn10 in B. licheniformis also has not been own either. Thus, a spontaneous non-mucoid derivative of the B. licheniformis was obtained first. Shuttle vector pHV1248 containing mini-Tn10 was introduced into the non-mucoid derivative by the method of protoplast transformation. The resulting transformant was reverted to the wild mucoid phenotype, and then mutated randomly with the mini-transposon by heat induction. Auxotrophs requiring arginine, lysine, or tryptophan were isolated by replica plating method. Southern blotting and DNA-DNA hybridzation analysis showed that these auxotrophs were generated by mini-Tn10 insertion into the chromosomal DNA. This method of transformation and mutation using pHV1248 would be useful for the generation of diverse mutants of B. licheniformis 9945a.(Received January 24,1997; accepted March 10, 1997)

• The Korean Journal of Mycology
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• v.17 no.4
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• pp.209-213
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• 1989

Transformation of an auxotrophic requirement for uracil in Pleurotus florida P101 has been achieved using chimeric vector containing Aspergillus nidulans ans 1, and Neurospora crassa pyr 4 DNA. Protoplasts of $Ura^-$strains of P. florida were incubated with plasmid pDJB3 containing the cloned pyr 4 gene in the presence of polyethylene glycol and $CaCl_2$. Transformants could grow on MMM showing mitotical stability. Southern hybridization analysis of DNA isolated from transformants showed that the Neurospora pyr 4 gene and vector sequence might be integrated into the P. florida chromosomes. As the transformants were monokaryon, each transformant was mated with the other monokaryon. Fruitbody shape of untransformant was eroded type but those of transformants were eroded type, funnel type, plane type and ungrowing cap type.

• Korean Journal of Plant Taxonomy
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• v.43 no.3
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• pp.188-195
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• 2013

The morphological characters and scientific names of Korean Artemisia sect. Absinthium were reviewed based on the type specimen, the original description, observation of the living materials and voucher specimens. The form of the habitat, the structure of underground, the leaf shape and capitulum features were valuable characters in identifying the taxa. The sect. Absinthium in Korea was classified as three taxa of A. sieversiana, A. lagocephala, A. fauriei. The illustration and description of each taxa were provided. Particularly, A. fauriei in this section with hair on receptacle and A. fukudo in sect. Abrotanum without hair on receptacle grow in mixed groups on the West shore of Korea. Two species with populations which grow in mixed groups, was observed as being putative hybrid individuals based on morphological characters.

• Applied Biological Chemistry
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• v.35 no.5
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• pp.361-366
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• 1992

The antagonistic bacteria, showing distinguished effect against Fusarium oxysporum and Rhizoctonia solani were isolated from the rhizosphares of horticultural plants and identified as Bacillus subtilis. The strains were studied for their chracteristics of biochemistry, physiology, antagonistic effect against plant pathogenic fungi, and growth promoting effect on horticultural plants. The Bacillus thuringiensis(BT) HD-1 toxin gene was introduced into these B. subtilis. The BT toxin genes on chromosome of the bacteria were identified by southern blotting, but its proteins were not detected by SDS-PAGE. These transformed bacteria showed growth promoting effect and showed also insecticidal and antagonistic effects against Bombix mori and fungi F. oxysporum and R. solani but not against nematode Bursaphelenchus xylophilus.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.3-3
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• 2020

최근 지구온난화로 인한 기후변화로 인해 지리적 분포가 한정적인 한반도 구과식물들은 점점 서식지 내 생육에 위협을 받고 있다. 이러한 구과식물의 지속이용가능한 보전을 위해서는 각 종별 형질 특성 및 분포, 생육정보 등의 기초정보 구축이 필요할 것이다. 구과식물(Pinidae, Conifers)은 전 세계적으로 나자식물(Gymnosperms)의 3/5정도인 630여분류군이 분포하는 것으로 알려져 있다(Farjon, 2008). 은행나무나 소철나무류 등 다른 나자식물과 비교하여 여러 복잡한 구조로 이루어진 구과(웅성, 자성 구과)를 형성하고, 잎은 대부분 바늘형으로 단순하며, 상록성인 특징 등으로 구분된다. 우리나라에 분포 및 식재되고 있는 나자식물 중 은행나무와 소철나무를 제외한 모든 나자식물이 구과식물에 속한다. 구과식물이 전 세계적으로 넓은 분포역을 갖지만, 종별로는 제한적인 분포를 갖는 것들이 많이 나타난다. 우리나라에 자생하는 구과식물 대부분도 동북아시아에만 분포하는 종들이 대다수이다. 한반도 구과식물은 총 5과 12속 23종 5변종 총 28분류군으로 확인되었다. 소나무과는 5속 12분류군으로 구상나무와 분비나무의 형태적 중복형질이 확인되었으며, 측백나무과는 3속 9분류군으로 눈향나무를 변종에서 종 수준으로 적용하였고, 어청도에서 해변노간주의 분포를 확인하였다. 개비자나무과는 1속 3분류군으로 수형에 따라 큰개비자나무, 눈개비자나무, 개비자나무로 구분하였다. 주목과는 비자나무속을 포함하여 2속 3분류군으로 설악눈주목에 대한 세부적인 분류학적 재검토가 필요할 것으로 판단한다. 나한송과는 1속 1분류군으로 신안군 가거도에서 나한송의 자생지를 확인하였다. 구상나무와 분비나무의 형태적 구분형질이 모호함에 따라 집단별 유전정보 분석을 통한 계통지리학적 연구를 수행하였다. 분석을 통해 구상나무는 빙하기에 북부지역의 조상종이 남하하여 분화되고, 후빙기에 중부지역까지 분포확장이 이루어진 것으로 판단된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.51-51
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• 2020

콩의 원산지는 만주를 중심으로한 동북아시아를 원산지로 추정하고 있으며, 동아시아인 한국, 중국, 일본의 주요 식재료로 다양하게 이용되어왔다. 콩은 단백질과 지방의 공급원으로서 뿐 아니라 최근에는 콩의 생리활성 물질이 항암효과, 골다공증, 신장질환과 같은 질병예방에도 효과가 있는 것으로 알려지면서 활용가능성이 높아지고 있다. 본 연구는 유전자원센터에 보존 중인 콩 수집단에서 농업형질, 기초정보 등을 토대로 핵심집단을 작성하고 그 중 한국, 중국, 일본 수집단 각 425, 93, 42자원의 개화일수, 생육일수, 주당협수, 100립중, 주당수량과 같은 양적형질을 비교하였다. 개화일수와 생육일수는 중국수집 자원에서 평균38일, 107일로 가장 빠르며, 한국수집 자원은 평균 53일, 127일로 가장 늦었다. 협당립수는 중국수집 자원에서 협당 평균3립으로 가장 많았으며, 한국과 일본 수집자원은 각 2.3과 2.4립으로 유사하였다. 주당협수는 한국 수집자원에서 주당 평균 327.4개로 가장 많고 일본이 248.0개 중국이 224.4순으로 조사되었다. 100립중은 일본 수집 자원이 평균 33.6g으로 대립이 많고 한국 26.5g , 중국 21.1g 이었다. 주당수량은 한국 수집자원이 평균 119.8g, 일본 84.2g, 중국 76.8g순이었다. 이들 5개 양적형질을 토대로 군집분석한 결과 한국 자원은 대다수가 C3 그룹에 속하고, C1 그룹에 일부가 속하였으며, 일본 자원은 C3 그룹과 C2 그룹에, 중국 자원은 C2 그룹에 속하여 한국과 일본자원이 유사하고 중국자원과는 차이를 보이는 것으로 나타났다. 이러한 특성조사 결과는 자원의 수집, 도입시 다양성 확보를 위한 기초정보로 이용할 수 있다.