• Korean Journal of Environment and Ecology
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• v.30 no.4
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• pp.658-675
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• 2016

The environmental characteristics of the habitats, the population structures, ecological niche, life traits and the population demography are used as an essential informations to the effective conservation plans about the target species. We first confirmed a total of 37 populations through 12 years of investigation on the extents, and investigated the environmental factors of habitats and the population structures of the 25 cohorts in 12 regions among them. And, we investigated the population dynamics targeting at 8 cohorts in the population of Daegwanryeong-myeon, Pyeongchang-gun, Gangwon-do. Along with this, we conducted 4 years of cultivation experiments and how pollinators affect the success of reproduction. The results shows that the Paeonia obovata Maxim. was found in a relatively wide range of habitats. For the population structures of the 25 cohorts, there were considerable differences among cohorts, including a case which showed relative stable structure and an extreme case in which only adults were found. The factor that affected the population dynamics during the investigation was thought as a forest gap created in the tree layer. In the cultivation experiment, seedlings grew well in a less soil organic matter. The seed setting rate and the number of seeds generated by visiting pollinators were higher trends. In conclusion, to understand the distribution of the Paeonia obovata Maxim., there needs to be investigated on the other factors that are involved in dispersal factors other than environmental factors, and thus we suggest the necessity of the research on the dispersal vectors. The current Paeonia obovata Maxim. population was thought as a process of expansion after the rapid reduction of the extents in the past. For the effective conservation of the Paeonia obovata Maxim., an endangered plant, we recommend to understand the species through the spatio-temporal expansion, and maintain the health of the ecosystem based on it.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.3
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• pp.286-292
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• 1986

For the elucidation of saponin synthesis during ontogeny changes of ginsenosides and free sugars in seeds during stratification and seedlings in early growth stage were investigated with high performance liquid chrom-atography. Embryo plus endosperm at 40-day stratification showed 80% decrease of total saponin, disappear-ance of Rc, Rb$_2$ and Rb$_1$ and appearance of Rg$_3$ (probable) and 20-Glc-Rf (probable). Leaf ginsenoside F$_3$ was found not in fruit plup but seed and decreased during stratification. Both decomposition and synthesis of saponin seemed to occure during stratification. Ginsenosides in endosperm and embryo might be originated from fruit pulp by penetration. In seedling saponin appeared first in shoot and in root about one month later. Ginsenoside Rc, Rb$_2$, Rb$_1$ appeared in root at the last investigation (June 30) indicating normal saponin synthetic capacity of root. Saponin synthetic rate was twice in leaf than in root. Leaf ginsenoside F$_3$ was found in seedling root. Root saponin Rg$_3$ and 20-Glc-Rf were found in leaf and stem in seedling and decreased with growth suggesting that rate saponin is not such in certain growth stage. Total saponin content was negatively correlated with PT/PD in seeds and arial parts of seedling due to greater change of PD. than PT. Seed at 70days stratification showed high sucrose content. In seedling glucose was main sugar in stem all the while and sucrose in root at early stage while glucose, fructose and sucrose were found in leaf.

• Journal of Mushroom
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• v.18 no.3
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• pp.274-279
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• 2020

This study was conducted to investigate the characteristics of the medium used on the composting step, comparing the excavator agitator with the self-propelled turner. The temperature of the outdoor composting medium tended to increase rapidly after flipping in the turner. The late composting medium temperature was maintained at the excavator treatment area (farm practice), and the late composting effect progressed. During the field composting stage, various microorganisms such as Bacillus spp., Actinomycetes, fluorescent Pseudomonas spp., and filamentous fungi were distributed in the medium, and the density of aerobic bacteria involved in the decomposition of the medium was increased. Under high-temperature composting conditions, blue fungi, and mesophilic actinomycetes were inhibited or killed. Thermophilic actinomycetes, which play an important role in decomposing organic matter, showed higher densities than those observed in farm practices in the self-propelled turner process. The length of rice straw was slightly shorter when the self-propelled turner was used, and the water content did not show any significant difference between treatments. The a and b values tended to increase as the inverter was turned over. The CN ratio of the composting broth was lowered from 23.1 to 16.2 for the 5th turnover in the context of farming practices, and from 23.3 to 16.9 in the context of the self-propelled turner. The yield of each treatment was increased by 20% in 1 period, 28% in 2 periods, and 26% in 3 periods; the overall yield was 23%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.2
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• pp.328-332
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• 2014

A survey was conducted on the isolation of lactic acid bacteria with antibacterial activity to extend kimchi shelf-life. Antibacterial activity was tested against bacteria associated with acidification of kimchi, including Lactobacillus plantarum, Pediococcus pentosaceus, and Lactobacillus sakei, using agar-well diffusion assay. Two isolates from kimchi were identified as Lactococcus lactis subsp. lactis and Lactobacillus brevis by 16S rRNA gene sequence analysis and API 50 CHL assay, and they showed antibacterial effects against indicator strains. The isolates displayed acid tolerance at pH 3.5, salt tolerance in 5% NaCl, and growth at $4^{\circ}C$. These result imply that the selected strains might be used to extend kimchi shelf-life as a potential starter.

• Journal of Korean Society of Forest Science
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• v.84 no.1
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• pp.77-86
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• 1995

To effectively modify tree function with genetic engineering, transgenes must be expressed at the proper level in the appropriate tissues at suitable developmental stages. Toward understanding the spatial and temporal expression of transgenes in woody plants, transgene expression was evaluated in three greenhouse-grown, transgenic lines of Populus alba ${\times}$ P. grandidentata hybrid clone 'Hansen'. All transgenic poplar lines possess constructs containing the bacterial nopaline synthase(nos) promoter linked to a neomycin phosphotransferase II(NPT II) selectable marker gene. In addition, each transgenic poplar line contains one of the following gene constructs : 1) a wound-inducible potato proteinase inhibitor II (pin2) promoter linked to a chloramphenicol acetyltransferase(CAT) reporter gene. 2) a nos promoter linked to a PIN2 structural gene : or 3) a Cauliflower Mosaic Virus 35s promoter linked to a PIN2 structural gene. Polymerase chain reaction(PCR) was used to verify the presence of foreign genes in the poplar genome. Enzyme-linked immunosorbent assays(ELISAs) were used to evaluate organ specific expression of the nos-NPT II construct. NPT II expression was detected in leaves, petioles, stems, and roots of transgenic poplar, thereby indicating that the nos promoter is potentially effective for general constitutive expression of transgenes. NPT expression varied among transgenic poplar lines and among organs for one transgenic line, Tr15. With Tr15, NPT II levels were highest in older leaves and petioles. These results indicate that screening of several transgenic lines may be required to identify lines with optimal transgene expression.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.32 no.3
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• pp.110-120
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• 2014

In this study, Chwibyeong studied only in the literature on the basis of the composition method through the book, Imwonkyungjeji. I tried to represent through the installation of facilities and planting of trees. I have obtained the following results. First, we presented the basic direction of Chwibyeong represented by analyzing the composition methods in the literature. Second, represented the Chwibyeong based on the results of the analysis of the literature. I was and comparative analysis of the content analysis literature the specificity of the method of manufacturing facilities and growth characteristics of trees that have been displayed during the represent process. Third, we derive how to manage trees that are grasped through the Chwibyeong represent process, how to manage the facility, such as the installation method. Currently, Chwibyeong is restored in the front of Changdeokgung Juhapru pavilion, but showing the appearance different from the appearance of Chwibyeong shown in the literature. Therefore, after the 18th century, prototype representation of Chwibyeong is intended to be able to find the traditional and the uniqueness. It has been used as an element of the garden of Korea, and intended to be an important role to find the flavor of the Korean garden.

• Korean Journal of Environmental Agriculture
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• v.3 no.2
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• pp.55-61
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• 1984

농업(農業)은 동식물(動植物)을 대상으로 하는 응용생태학(應用生態學)의 한 분야(分野)로 그의 발전과정(發展過程)은 환경(環境)과 밀접(密接)한 관계가 있다. 환경조건(環境條件)의 다양성(多樣性)은 대상생물(對象生物)의 종류(種類)를 풍부하게 하고 순화(馴化)된 생물(生物)의 사양(飼養) 과정에서 다양(多樣)한 농업형태(農業形態)를 발전시켰다. 생웅계(生熊系)는 자연(自然)의 한 단위로 구성요소(構成要素)들은 기능적(機能的)으로 통일성(統一性)을 유지하고 있다. 농생태계(農生態系)는 생산(生産)을 목표(目標)로 하는 동식물(動植物)을 중심(中心)으로 하는 생태계(生態系)로 자연생태계(自然生態系)와 기본적(基本的) 성질(性質)은 동일하나 인위적(人爲的) 요소(要素)가 크게 작용하고 있는 특리(特異)한 생태계(生態系)이다. 작물(作物)이나 가축(家畜)은 수천년에 걸친 생산력(生産力)의 증대(增大)를 목표(目標)로 인위적(人爲的)인 선발을 거친 것으로 자연조작하(自然條作下)에서 심한 경쟁(競爭)을 거치면서 진화(進化)한 야생생물(野生生物)들에 비하면 선천적(先天的) 포약성(胞弱性)을 갖고 있어 인간(人間)의 보호(保護)(에너지보조(補助))를 필요로 하고 있다. 농생태계(農生態系)는 공간적(空間的) 격리(隔離)와 구성종(構成種)의 단순화(單純化)로 공간적(空間的) 연쇄성(連鎖性)과 종다양도(種多樣度)가 큰 자연생태계(自然生態系)에 비하면 외적(外的) 충격에 대한 내부적(內部的) 완충능력(緩衝能力)이 약하다. 더우기 농생태계(農生態系)는 시간적(時間的) 연속성(連續性)이 없어 극상천이(極相遷移)단계에 달할 수 있는 시간적(時間的) 여유가 없어 생물상호간(生物相互間)의 관계는 초기(初期) 천이(遷移)단계의 특성(特性)인 종다양도(種多樣度)가 낮고 직선적(直線的)이고 단순(單純)한 먹이연쇄성(連鎖性)을 갖고 있다. 생물군집(生物群集)의 천이진행(遷移進行)에 따르는 종다양도(種多樣度)의 증가(增加)는 생물군집내(生物群集內) 현재량(現在量) 증가와 더부러 먹이 연쇄상(連鎖相)을 직선상(直線狀)에서 망상(網狀)으로 변화시키고 주된 물질(物質)${\cdot}$유전회로(流轉回路)를 포식(捕食)먹이 연소회로(連銷回路)에서 부식(腐食)먹이연쇄회로(連鎖回路)로 전환시켜 생태계(生態系)의 물질(物質)은 생태군집내(生態群集內)에 보존(保存)하는 능력(能力)을 증대(增大)시키고 토양(土壤)의 물리(物理) 화학적성질(化學的性質)을 개선하여 계내(系內) 물질(物質)의 유실(流失)을 억제하기도 한다. 농생태계(農生態系)는 생산성(生産性)의 증대(增大), 관리(管理)의 편의(便宜) 기타의 이유(理由)로 구성종수(構成種數)를 인위적으로 제한(制限)하고 관리(管理)를 통하여 발아(發芽), 생장(生長), 개화(開花)등 여러가지 생물학적(生物學的) 현상을 극도로 균질화(均質化)시키고 있어 어떤 생육단계(生育段階)의 생물(生物)이 넓은 면적(面積)에 동시에 존재하게 되는데 이것은 외적조건(外的條件)의 변동(變動)에 대한 감수성(感受性)을 증대시킨다. 이와 같은 자연생태계(自然生態系)와 농생태계(農生態系)의 생태학적(生態學的) 특성(特性)에 관한 이해(理解)와 인식(認識)은 농생태계(農生態系)의 생산성(生産性) 증대(增大)와 영구적(永久的) 유지(維持)를 위한 합리적(合理的) 관리(管理)를 위한 기본(基本)이 될 것이다.

• Korean Journal of Food Science and Technology
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• v.31 no.3
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• pp.771-777
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• 1999

The structural function of three egg membrane layers and cuticle layer, and the effectiveness of 5 film coatings (chitosan, starch, gelatin, dextrin, mineral oil) on the prevention of Salmonella enteritidis penetration was investigated using confocal scanning laser microscopy (CSLM). Diameters of outer membrane fibers, inner membrane fibers and limiting membrane particles in eggshell were $1.5{\sim}7.2$, $0.8{\sim}2.0$ and $0.1{\sim}1.4\;{\mu}m$, respectively and average thicknesses were 10.0, 3.5, $3.6\;{\mu}m$, respectively. Average thickness of cuticle layer was $6.0\;{\mu}m$ and cuticle layer covered $40{\sim}80%$ of total eggshell surface. Average coating films thickness for chitosan, starch, gelatin, dextrin and mineral oil were 2.2, 2.5, 3.9, 3.6 and $5.0\;{\mu}m$, respectively. After immersion process eggshell surface was almost completely covered by coating films. Chitosan coating was most effective among 5 film coatings in inhibiting growth of Salmonella enteritidis. Penetration process of Salmonella enteritidis through eggshell was investigated by multicolor imaging using CSLM and plate counting. Cuticle layer was the most important structure in blocking the penetration. Among 5 film coatings, chitosan showed the best and similar effectiveness with cuticle layer.

• Proceedings of the KGS Conference
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• 2002.05a
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• pp.99-105
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• 2002

변화의 속도가 느려서 장기지속적인 전체사의 바탕을 형성하고 있는 지리적 환경에 대한 적응결과로서의 지표경관을 도면상에 표현한 고지는 당시대인들의 세계관과 공간인식을 반영하고 있다. 인간을 둘러싼 지리적 환경은 장기적으로 벼하는 구조이다. 장기지속의 구조사는 지리지와 고지도에 잘 표현되어 있다. 지리지와 고지도에 대한 문헌조사와 현지야외답사를 통하여 경관을 구체적으로 분석하고 지역성과 한국전통지역사회의 원형을 파악하고자 한다. 기존의 연구자들에 의하여 정상기의 동국지도, 김정호의 대동여지도, 북방영토인식, 군현지도, 시대상의 복원과 지리환경 인식, 회화의 측면에서 본 고지도, 지명과 영토문제, 서양식 세계지도의 전래과정등의 연구주제들에 대하여 많은 연구가 이루어졌다. 그러나 기왕의 연구는 고지도에 대한 전체적인 현황 파악과 해제, 전체적인 유형 분류, 특정주제에 대한 고지도 이용 등에 초점이 맞추어져 있었고 구체적인 지역사회에 대하여 고지도를 통시대적으로 비교 분석한 연구는 드문 편이다. 사라져가고 있는 전통경관과 자연환경의 영향을 많이 받은 촌락경관이 남아 있는 지역사회경관에 대한 고지도와 지리지를 활용한 연구가 시급하다. 고지도는 옛 지명, 산천, 도로, 역원, 행정구역, 역사적 위치의 파악과 문화경과 복원의 기초자료로서의 의미가 크므로 지역사회 단위의 연구가 필요하다. 19세기 말엽에 편찬된 읍지와 지도는 서양과 일본의 침략으로 변모되기 이전의 한국 각 지역의 모습을 추적하고 복원하는데 긴요한 역사적 자료이다. 현대 한국지역사회의 바탕을 이루고 있는 지난 400년간의 지역변화를 고지도와 지리지.읍지를 통하여 살펴봄으로써 변한 것과 변하지 않는 것을 분석하여 시대상의 복원과 지역사회의 정체성 확립에 일조하고자 한다.곡물의 여러 줄기가 합수하고 물이 역류하지 않으며 잘 감아도는 곳으로 표현할 수 있다 이러한 명당의 기본적인 원리는 장풍(藏風)과 득수득파(得水得破)이다. 장풍과 득수란 '국'(局), 즉 산줄기와 수계로 이루어진 일정한 범위 속에 만물이 생태계 속에서 살아가는 모습인 '체형(體形)'의 의미가 나타나야 한다는 것이다. 그러나 이러한 의미는 시대에 따라 달라질 수 있는 것이다.면적의 52%를 점유하여 감자 재배지의 핵심지를 이룬다. 4. 한국 감자 재배지역 중심지의 지리적 특징은, 높은 산지지역의 산록완사면에 밭작물로 재배된다는 점과 교통이 불편한 지역으로서 도시화와 산업화 지역의 그늘 지역이 대부분이다. 강원도의 감자 재배지는 감자재배에 적합한 자연환경과 화전농업의 전통, 감자 재배기술의 전파, 중앙정부와 지방정부가 지원하는 각종 연구소 분포와 영농지도, 씨감자 생산과 협동조합의 판로 개척, 도로 개설과 포장 등의 인문지리적 요인이 영농조건을 개선하고 감자 판매를 위한 시장접근을 용이하게 하여, 남한 최대의 감자 재배지역을 형성하였다. 제주도는 산지지형과 따뜻한 기온으로 2기작이 가능하고, 감자가공 공장설립과 교통발달에 따른 육지 시장과의 접근이 용이해졌기 때문에 남한에서 2번째로 큰 감자재배지역이 되었다.(요약 및 결론에서 발췌)그람양성균에서 효과적이었으며, 농도별 항균력시험 결과 농도가 증가할수록 비례하여 저해율도 증가함을 알 수 있었다. 첨가농도를 달리하여 미생물의 생육도를 측정한 결과, fraction II磎꼭\ulcorner경우 그람양성균에 대해 500 ppm 이상에서 뚜렷한 증식억제효과를 나타내었다.서 뚜렷한 증식억제효과를 나타내었다.min/+}$계 수컷 이형접합체 형질전환 마우스에 AIN-76A 정제사료만을 투

• Applied Biological Chemistry
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• v.42 no.1
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• pp.12-19
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• 1999

To improve biopolymer productivity and properties of Bacillus strains, protoplast fusion was performed between biopolymer producing Bacillus subtilis K-1 and lactose utilizing Bacillus coagulans. The results were as follows; Protoplasts mixed in fusion fluid containing 33% PEG 6000, 1% PVP and 10 mM $CaCl_2$ were reacted for 5 min at $37^{\circ}C$ and then centrifused protoplasts were directly overlaid on the selective media containing $100\;{\mu}g/ml$ antibiotics and incubated for 3 days. At this conditions, the frequency of protoplast fusion was generally in the range of $4.6{\times}10^{-5}\;to\;1.8{\times}10^{-7}$ in ratio. Segregation ratio was observed between 1 to 6% indicating genetic stability of all the fusants. Fusants growth were also observed on the media contained amino acid and antibiotics as required marked materials. DNA contents of the selected fusants were 1.6 to 2.7 times more than that of parent strains. With observation by TEM microscopy, spherical protoplasts were first released from the swollen parental cells and then contracted to fuse in the process of fusion. And fused cells were observed representative vesicle. Originally, the parental cells were observed as in the morphology of thick-walled and double membrane-surrounded rod shape with TEM microscopy.