• Title/Summary/Keyword: 생물 모니터링

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생물 반응기를 이용한 글루타치온의 생산 및 모니터링

  • Kim, Chun-Gwang;Seo, Guk-Hwa;Son, Ok-Jae;Lee, Jong-Il
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.189-191
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    • 2003
  • 의학 및 해양 수산 분야에서 광범위하게 사용되어지고 있는 글루타치온을 생산하는데 보다 적절한 배양 배지를 찾기 위해 당 농도, cysteine 농도변화에 따른 글루타치온의 생산성을 살펴보았으며 특히 형광 센서를 이용하여 글루타치온의 생산 특성을 모니터링하였다.

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On-line Monitoring of Glucose and Starch by a Flow Injection Analysis Technique (흐름주입분석 기술에 의한 글루코우스와 전분의 온라인 모니터링)

  • 김준홍;박돈희;이종일
    • KSBB Journal
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    • v.16 no.5
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    • pp.459-465
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    • 2001
  • The on-line monitoring technique for the concentrations fo glucose and starch by FIA(Flow Injection Analysis)system was studied. Glucose oxidase(GOD) and amyloglucosidase(AMG) were immboilized on VA-Fpoxy carrier and integrated into the FLA system. The pH, buffer flow rate and temperature were optimized and the effects of salts and metabolites dissolved in the sample on the activity of immobilized enzyme were investigated. GOD-FIA and AMG/GOD-FIA were applied for the on-line monitoring of the glucose and starch in a simulated bioprocess. The on-line measurements of glucose concentrations by GOD-FIA agreed with off-line data well and the AMG/GOD-FIA with single cartidge system took and advantage over the FIA system with two separated cartridges for the on-line monitoring of starch concentrations.

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