• Proceedings of the Korea Contents Association Conference
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• 2010.05a
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• pp.185-187
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• 2010

이 연구는 9.11 테러 이후 발생한 뉴테러리즘 중 가장 잔혹한 생물테러리즘을 예방하기 위한 대비체계중 검증체계를 구축한 실태를 확인한 논문이다. 생물테러리즘을 위기로 인식하고 예방하기 위해서는 대비체제를 잘 구축하여 관리하면서 실질적으로 가동하는 여부를 확인하는 활동이 필요하다. 생물테러리즘을 예방하기 위한 체제는 우선 테러리즘을 예방하기위한 법체계가 구축되어야 하고, 생물테러리즘에 사용되는 전구물질을 통제하는 법체계가 갖추어져야 한다. 그리고 이러한 법체계를 수행하기 위한 각종 조직들이 갖추어지고, 조직들이 활성화되어야 한다. 본 연구결과 한국에는 테러리즘을 통제하는 상위법령이 없는 상태이나 국제적인 요구에 부응하기 위해, BWC의 협약에 언급된 직 간접적인 이전금지, 제조, 획득, 권유, 조장 등을 금지하기 위해 생물테러리즘을 예방하기 위한 여러 법령을 제정하고, 이를 시행하기 위한 조직이 구성되어 활동 중이다. 또한 생물테러리즘에 사용될 수 있는 물질과 장비들은 산업계와 학계에서 연구목적으로 또는 의료약품 생산을 목적으로 필요로 하는 것이므로 생물학 물자 및 장비의 유통질서를 확립하고 확인하는 것이 필요하다. 이를 위한 조직이 활동 중이다. 생물무기 보유여부의 신고, 이의 폐기, 평화적 용도로의 전환사용 등에 대해서도 추진 중이다. 또한 이를 국제적으로 확인하는 체제를 구축하기 위한 국제적 협력이 계속 되어야 할 것이다.

• Proceedings of the Korean Information Science Society Conference
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• 2005.11b
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• pp.253-255
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• 2005

최근 생물학 문제를 해결하기 위하여 생명 정보 분야에서 많은 컴퓨터 기술들이 응용되고 있다. 플라보노이드 정보는 항암, 항산화 효과가 있는 유용한 물질인 플라보노이드의 연구와 관련된 정보를 분석하고 관리하는 컴퓨터 기술 기반의 응용 기술이다. 현재 플라보노이드 정보 관련 연구는 일부에서 수행되고 있으며 플라보노이드의 다양한 정보를 효율적으로 저장 검색 할 수 있는 시스템은 부족한 실정이다. 플라보노이드 물질에 관한 정보를 더욱 효과적으로 저장 관리하고 관련된 연구자들 간에 정보를 쉽게 공유할 수 있도록 본 논문에서는 플라보노이드 정보를 위한 그리드 웹 포탈 시스템 구조와 관련된 데이타 모델을 제안한다. 본 논문에서 제안한 시스템과 데이타 모델의 유용함을 보이기 위해 웹 기반으로 플라보노이드 정보 그리드 포탈을 구현한다.

• Development and Reproduction
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• v.6 no.1
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• pp.25-30
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• 2002

Endocrine disruptors have been reported to adversely affect reproduction and embryonic development in wild animals. One of the major abnormalities observed during early embryonic development is cellular fragmentation. In this study, we exposed mouse preimplantation embryos to PCB, BPA and DDT in vivo or in vitro. Embryos exposed to endocrine disrupter showed a variety of morphological abnormalities such as fragmentation, irregular blastomeres and cracked empty zonae pellucidae. To investigate the levels of gene expression related which genes contribute to apoptosis in preimplantation mouse embryos, we carried out the reverse transcription polymerase chain reaction to assess mRNA levels far apoptotic gene. Bcl-2, bad and bax expression levels were compared between control group and endocrine disrupter treated group. Expression level of bcl-2 gene tended to be lower in the treated group than control while expression levels of bad and bax genes were higher in the treated group. Results of this study may provide a useful tool for rapidly screening developmental toxicants in preimplantation embryos exposed to endocrine disruptors in vivo or in vitro.

• Development and Reproduction
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• v.2 no.2
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• pp.115-133
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• 1998

Recently public concerns about the endocrine disrupting chemicals (EDC) have resulted in the increased legistrative and regulatory attentions in many countries. Endocrine disrupter is an exogeneous chemical interfering the synthesis, transport binding, action or elimination of natural hormones in the body, which are responsible for the maintenance of homeostasis, reproduction, development and/or behavior. Reported possible harmful effects on human and animal lives, possible developmental anomalities, reproductive malfunctions and behaviors, and the ways of EDC accumulation in animal kingdom are reviewed. The current scientific papers and knowledges on the global contamination of EDCs demonstrates the conclusive data that EDCs have the potentials to cause the calamitous extermination of human and animal species by the global contamination.

• The Korean Journal of Zoology
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• v.22 no.2
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• pp.43-53
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• 1979

Variations in expressions of unique genes during oogenesis and early embryogenesis of a tubiculous polychaete were studied by determining the extents of gene transcriptions by sequential DNA-RNA molecular hybridizations. The genes which had been activated in the early stages of oogenesis (previtellogenesis) were gradualy suppressed during the subsequent stages of oogenesis. The transcripts that had been synthesized upto the stages examined were utilized and degraded throughout the vitellogenic stages, and thus, the amount of the transcripts remaining in the fully-grown oocytes was much smaller than that of the previtellogenic oocytes. During the post-fertilization period new genes were transcribed even in the 4-8 cell stage embryos, and the extent of transcription of new genes continues to increase at least upto the trochophore stage.

• Development and Reproduction
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• v.8 no.1
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• pp.1-9
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• 2004

The potential importance of proteomic approaches has been clearly demonstrated in other fields of human medical research, including liver and heart disease and certain forms of cancer. However, reproductive researches have been applied to proteomics poorly. Proteomics can be defined as the systematic analysis of proteins for their identity, quantity, and function. It could increase the predictability of early drug development and identify non-invasive biomarkers of toxicity or efficacy. Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis(2DE) and MALDI-TOF(matrix-assisted laser desorption ionization-time of flight) MS(mass spectrometry) or protein chip array and SELDI-TOF(surface-enhanced laser desorption ionization-time of flight) MS. In addition understanding the possessing knowledge of the developing biomarkers used to assess reproductive biology will also be essential components relevant to the topic of reproduction. The continued integration of proteomic and genomic data will have a fundamental impact on our understanding of the normal functioning of cells and organisms and will give insights into complex cellular processes and disease and provides new opportunities for the development of diagnostics and therapeutics. The challenge to researchers in the field of reproduction is to harness this new technology as well as others that are available to a greater extent than at present as they have considerable potential to greatly improve our understanding of the molecular aspects of reproduction both in health and disease.

• Journal of Naturopathy
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• v.10 no.1
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• pp.42-47
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• 2021

Purpose: This study investigated the antioxidant activities of water extracts from Hibiscus petals, Moringa oleifera gymnosperm, and Nipa fruticans wurmb. Also, the possibility of their use as a functional cosmetic material and food were searched. Methods: We extracted Hibiscus petals, M. oleifera gymnosperm, and N. fruticans wurmb with water. And then, we measured the content of total polyphenols and flavonoids and the ability to scavenging free groups of ABTS and DPPH to study the antioxidant function. The toxicity of samples evaluated by measuring cell viability. Results: The polyphenol content of the water extract of N. fruticans wurmb was 109 ㎍/mg, which was significantly higher than that of Hibiscus petals (13 ㎍/mg) and M. oleifera gymnosperm (19 ㎍/mg). Radical scavenging ability was also excellent in N. fruticans wurmb, and the cytotoxicity test results of the samples were similar. Conclusions: The water extracts showed antioxidant activity to use for cosmetic materials or natural healing foodstuff.

• Korean Journal of Optics and Photonics
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• v.35 no.4
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• pp.135-142
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• 2024

Biomedical imaging technologies refer to imaging techniques used in biological research and medical technology that are essential for exploring biological processes, structures, and conditions. They also play a crucial role in the early diagnosis of diseases and the development of treatments. Optical imaging technologies, in particular, are the most widely used and actively researched in biological studies. The major obstacles to technological advancement are the limitations in resolution and light penetration depth. Recently, many technologies have been studied to overcome these limitations using metamaterials. These are materials that can freely manipulate the properties of light through the regular arrangement of nanostructures and have established themselves as innovative tools in the imaging field. This article aims to provide a detailed introduction to the working principles and key applications of these technologies.

• Development and Reproduction
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• v.12 no.3
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• pp.267-274
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• 2008

Differentiation of blastocyst is critical step for implantation and is under the control of regulation factors originated from embryo or reproductive tracts. The sequential communication with those factors is suspected as critical events for differentiation. It has been suggested that intracellular signaling pathways activated by calcium is essential in differentiation of blastocyst. Previously, it was known that concanavalin A (Con A) increase the levels of free calcium in blastocyst stage. However, Con A can not accelerate the hatching, although heparin-binding epidermal growth factor-like growth factor (HB-EGF), a modulator of calcium level, accelerate the hatching of blastocyst. In this study, it was investigated whether Con A or prostaglandin $E_2$ ($PGE_2$) can modulate the differentiation of blastocyst. Con A accelerated the expansion of blastocyst in both 1 hr pulse treatment group and continuous treatment group. However, Con A significantly suppressed the hatching in both groups. The inhibition was significantly strong in continuous treatment group compared with 1 hr pulse treatment group. On the other hand, $PGE_2$ induced the increase the free calcium level, but did not accelerate the expansion. In addition $10{\mu}m\;PGE_2$ inhibited hatching. However, $PGE_2$ could accelerate hatching in Con A pretreated blastocyst. $PGE_2$ also caused the increase of free calcium level in Con A pretreated blastocyst. From these results, it is suggested that changes of the free calcium level induce a different calcium-mediated signaling pathways. In addition, sequential stimulation by signal molecules may triggers the cellular mechanisms for the differentiation of blastocyst.

• Development and Reproduction
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• v.1 no.1
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• pp.57-65
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• 1997

생쥐의 부정소에서 진행되는 정자 성숙과정 동안의 첨체반응 능력의 변화를 조사하였다. 자발적 첨체반응 및 난포액, 프로게스테론, 또는 A23187에 의해 유발되는 첨체반응은 모두 정자의 성숙에 의존저긍로 일어났다. 두부 부정소의 매우 적은 정자만이 난포액 및 프로게스테론에 반응하여 첨체반응을 일으켰으며 체부 및 미부 부정소간에 첨체반응율에 차이가 없었다. 반면 A23187 처리시 상당수의 두부 부정소 정자가 첨체반응을 진행하였다. 이러한 결과에서 두부 부정소를 거쳐 체부 부정소에 도달한 생쥐 정자는 첨체반응 능력을 획득하여 이 과정에서 정자의 원형질막 표면에서는 첨체반응을 유발하는 물질과 상호작용에 필요한 변화가 진행되는 것으로 사료된다. 반면 정자내로의 $Ca^{2+}$ 유입 후 진행되는 막융합과 첨체내용물의 분비에 필요한 능력은 두부 부정소 정자도 일부 갖고 있는 것으로 사료된다.