• Title/Summary/Keyword: 생물학적 활성

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Control of Ginseng Damping-off by Streptomyces sp. A3265 (방선균 A3265 균주에 의한 인삼 잘록병의 방제)

  • Woo, E-Eum;Lee, Gang-Seon;Lee, In-Kyoung;Choi, Jae-Eul;Yun, Bong-Sik
    • The Korean Journal of Mycology
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    • v.44 no.3
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    • pp.193-195
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    • 2016
  • Korean ginseng (Panax ginseng) possesses various biological and pharmacological properties. Damping-off is a critical disease on ginseng seedlings, which is caused by the fungal pathogens Rhizoctonia solani and Pythium sp.. This disease is generally controlled by the application of fungicides, but also biological control is an efficient and environmentally friendly way to prevent ginseng damping-off. In a previous study, we screened soil-borne bacteria with potential applications as biological control agents for ginseng damping-off and selected the bacterial strain Streptomyces sp. A3265, producing antifungal substances guanidylfungin and methylguanidylfungin. In this study, we investigated control efficacy of Streptomyces sp. A3265 against ginseng damping-off in the field. As a result, the incidence of damping-off was significantly reduced when soaking ginseng seeds in the culture broth of Streptomyces sp. A3265.

Isolation and Characterization of Denitrification Bacteria (탈질 세균의 분리 및 특성)

  • 차월석;최형일;이동병;차진명
    • KSBB Journal
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    • v.18 no.6
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    • pp.461-465
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    • 2003
  • Five denitrifying bacteria, which were identified as Pseudomonas sp., were isolated by the enrichment culture technique. The most effective denitrifying bacterium was named as Pseudomonas DWS, which was cultivated at anoxic condition. The optimal growth temperature and pH were 30$^{\circ}C$ and 7-8, respectively. Cell growth almost revealed a stationary phase at 18 hours after cultivation and nitrate was degrade 99.9% during this period. Therefore, it is suggested that Pseudomonas DWS could be effectively used for the biological treatment of wastewater containing nitrogen compounds.

Optical Resolution of Hexanol Derivatives, Synthesis of Optically Active Systhane from Them and Its Biological Activity (Hexanol 유도체의 순수이성질체로의 분할, 이를 이용한 광학활성 시스탄의 합성 및 생물학적 활성)

  • Im, Dai-Sig;Lee, So-Ha;Cheong, Chan-Seong
    • Applied Biological Chemistry
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    • v.46 no.3
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    • pp.240-245
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    • 2003
  • $({\pm})-2-(4-Chlorophenyl)-2-cyano-2-phenyl-1-hexanol$ (2) and acetate ester (3) were resolved by various lipases. (R) and (S)-systhane were synthesized by the resolved compound 2. The antifungal screening of (R), (S)-systhane and $({\pm})-systhane$ against wheat leaf rust and barley powdery mildew gave activity over 92% in concentration of 2 ppm, but (R)- and (S)-systhane were not more active than $({\pm})-systhane$.

Preparation and characteristics of Wet laid paper filter on pulp/activated carbon (습식공정을 이용한 펄프/활성탄 종이필터의 제조 및 특성)

  • Kang, Seung-Uck;Kim, Hyun-Chul;Kim, Wan-Jin;Jang, Hea-Jin
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2011.11a
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    • pp.43-43
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    • 2011
  • 현대인들은 생활환경에서 발생하는 각종 미세먼지 등의 입자상 오염물질 뿐만 아니라 각종 VOCs 등의 가스상 오염물질과 곰팡이, 박테리아, 바이러스 등 생물학적 오염물질까지 생활공간 내의 공기환경은 점점 악화 되고 있으며 공기 정화 등의 필요성이 증대 되고 있다. 이에 본 연구에서는 닥 펄프와 활성탄이 주성분인 Sheet를 Corrugation 장치를 통해 편파형(골판지)으로 제작하고, 다시 다층 구조로 적층된 골판지 sheet을 제작한 후 이를 두께 방향으로 일정 폭(20mm이하)으로 절단하여 필터부품의 소자로 이용하는 활성탄 필터의 제조 및 그 특성을 확인하고자 하였다. 닥펄프/펄프와 무기물의 혼합비, 해리시간 등의 변화를 주어 샘플을 만들었고, 강도, 평활성, 생산성 향상을 위해 첨가보조제 선정을 하였다. 이렇게 제조된 필터의 물리적 특성을 알아보고자 인장시험을 실시하였고, 주사전자현미경을 통하여 필터의 평활성과 표면을 관찰하였고 흡착성능 및 기능성을 VOCs 방출량, 탈취율, 항균도, 곰팡이 저항성 등의 실험을 통하여 알아보았다.

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Synthesis of 1-(4'-Thio-${\alpha},{\beta}$-D-ribofuransyl)-5-halogeno Uracils (I) (5-할로겐 치환 uracil 들의 4-Thiosugar Nucleosides의 합성 (제 1 보))

  • Kim Jack C.;Lee Euk-suck;Kim Chang-bae;Bobek Miroslav;Whistlee Roy L.
    • Journal of the Korean Chemical Society
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    • v.19 no.2
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    • pp.130-133
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    • 1975
  • The 4'-thio analogs of the 5-bromo-and 5-iodo-pyrimidine nucleosides were prepared by condensation of 2,3,5-tri-O-acetyl-4-thio-${\alpha},{\beta}$-D-ribofuranosyl chloride with the chloromercury derivatives of 5-halogeno-2,4-bis(trimethylsiloxy)pyrimidine, followed by the removal of the protecting groups. Although the biological activities of the 4'-thio derivatives are not greatly different from the corresponding 4'-oxygen analogs in this preliminary test, the fact that the 4'-thio analogs have comparableihigh activities, is of interest, and indicates the value of further biochemical examinations.

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Characteristics of Biosurfactant Producing Pseudomonas sp. G314 (생물 계면활성제를 생산하는 Pseudomonas sp. G314의 특성)

  • Shim, So-Hee;Park, Kyeong-Ryang
    • Korean Journal of Microbiology
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    • v.42 no.4
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    • pp.286-293
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    • 2006
  • Three hundred thirty two bacterial colonies which were able to degrade crude oil were isolated from soil samples that were contaminated with oil in Daejon area. Among them, one bacterial strain was selected for this study based on its low surface tension ability, and this selected bacterial strain was identified as Pseudomonas sp. G314 through physiological-biochemical tests and analysis of its 16S rRNA sequence. Pseudomonas sp. G314 showed a high resistance to antibiotics such as ampicillin, chloramphenicol, spectinomycin, and streptomycin, and heavy metals such as Li, Cr, and Mn. It was found that the optimal pH and temperature for biosurfactant production of Pseudomonas sp. G314 were pH 7.0 and $30^{\circ}C$, respectively. After seven hours of inoculated, the biosurfactant activity reached the maximum, and surface tension of the culture broth was decreased from 72 to 25 dyne/cm. The crude biosurfactant was obtained from the culture broth by acid precipitation, followed by solvent extraction, evaporation and then freeze drying. The CMC (critical micelle concentration) value of the crude biosurfactant was 20 mg/L.

Removal of Endocrine Disrupting Chemicals in Wastewater by Nitrifying Sludge (질산화 슬러지에 의한 폐수 중의 내분비계 장애물질 제거)

  • Lim, Kyoung Jo;Hong, Soon Ho;Chung, Jin Suk;Yoo, Ik-Keun
    • Korean Chemical Engineering Research
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    • v.47 no.6
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    • pp.775-780
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    • 2009
  • The efficacy of nitrifying sludge existed in biological nutrient removal process was examined for possible removal of endocrine disrupting chemical(EDC) in the effluent of wastewater treatment plant. Some of ammonia oxidizing bacteria causes ammonia oxidation mediated by ammonia monooxygenase(AMO) activity, which has low substrate specificity resulting in cometablic degradation of several chemicals. In this study, the removal of three model EDCs such as bisphenol A(BPA), nonylphenol(NP) and dibutyl phthalate(DBP) was studied in batch cultures using nitrifying sludge, BOD-oxidizing sludge with low nitrifying activity, and sterilized sludge. Nitrifying sludge showed higher initial removal rates in all batches of three EDCs when it was fed with ammonium as an energy source. The acclimation time was required for the removal of EDCs in batches using BOD-oxidizing sludge or nitritefed nitrifying sludge. That retardation seemed to attribute to the slow growth of cells using the EDCs while ammonium-fed nitrifying sludge could degrade EDCs through simultaneous cooxidation with ammonia oxidation. Sterilized sludge was also tested under the same conditions in order to find the contribution of physical adsorption to the removal of EDCs. About 10~20% of initial EDCs dose was removed when using sterilized sludge. Thus the biological activity is likely to play major role for the degradation of BPA, NP, and DBP rather than the physical adsorption from wastewater.

효율적인 Bioventing 공정을 위한 모니터링 시스템 개발

  • 문원재;오영진;왕성환;박태주
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2002.09a
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    • pp.359-361
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    • 2002
  • 국내에서는 1990년대 이후부터 주유소 및 유류저장시설 부근의 토양 및 지하수오염에 관한 법이 제정되었으며 그와 관련된 복원 및 정화 기술개발에 관한 연구가 진행되고 있다. 그 중 미생물학적 대사를 이용하는 생물학적복원기술(Bioremediation)은 유류로 오염된 지역에서 정화 및 복원방법으로 적용되고 있으며 또한 경제적인 대안으로 제시되고 있다. Bioremediation기법중 하나인 bloventing은 운전 및 처리비용의 감소와 효율의 향상적인 측면에서 여러 연구자들에 의해 수행되고 있다. 기존에는 오염토양에 대한 물리ㆍ화학적 분석과 미생물 활성에 미치는 인자의 측정이 주를 이루었다. 그러나, 최근에는 토양 가스내 $CO_2$ $O_2$의 측정ㆍ분석을 통한 분해율 평가와 탄화수소 분해에 대한 경과 및 복원시간을 예측하고자 하는 연구가 이루어지고 있는 중이다. 본 연구의 목표는 유류오염지역을 복원하기 위한 기술 중 생물학적 복원기술(bioremediation)의 하나인 bioventing 기술의 국내 적용가능성을 알아보고 궁극적으로 국내 실정에 적합한 bioventing공법의 개발을 목표로 한다. 이를 위해 lab-scale bioventing process를 이용하여 생부해를 최대화시키고 오염성분의 휘발을 최소화시키는 bioventing system의 최적운전 mode(연속식, 간헐식)를 개발하기 위해 공기공급량과 생분해량, 그리고 휘발되는 탄화수소양과의 관계를 평가하는 연구를 수행하였다.

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A Study on the Relationship between Obesity Factors and NK Cell Activity in White-Collar Females (사무직 여성의 비만요인에 따른 NK세포 활성도 관계 연구)

  • Sung, Hyun-Ho;Song, Chang-Sub;Choi, Kwang-Mo;Park, Chang-Eun
    • Korean Journal of Clinical Laboratory Science
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    • v.48 no.3
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    • pp.196-201
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    • 2016
  • An effective strategy for obesity intervention should include demographic, health-related, and work-related factors that are most relevant to the target population. Factors most strongly associated with obesity may differ for groups of individuals across industries or age categories. The purpose of this study was to provide current knowledge about the possible association between psychosocial job stress and immune parameters in blood. We assessed obesity-related parameters as well as the natural killer cell activity (NKCA) in female workers. There was a significant difference in the obesity-related blood parameters, i.e. fasting blood sugar level, according to age (p<0.05). Moreover, it showed a significant difference in NK cell activity between the obese and non-obese factor groups (p<0.05). Pearson correlation analysis demonstrated that NKCA was closely correlated with obesity related factors. In the future, it would have to study the biological changes which increase the activity of the immune factors to the immunological response.

미생물을 이용한 다용도 고형 탈취제의 개발

  • Kim, Yu-Jin;Lee, Eun-Jeong;Jeon, Mi-Uk;Kim, Cho-Hui;Park, Seong-Hun;Lee, Eun-Yeol
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.513-516
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    • 2001
  • This study was to develop of efficient microbial agent for malodor removal. Total ten strains of beneficial bacteria Bacillus sp., Pseudomonas sp., and photosynthetic bacteria were isolated and identified on the basis of their morphological and biochemical characteristics. The enzyme activities such as amylase, protease, lipase and cellulase of bacteria cells were measured. Furthennore, effective formulation procedure 、 ,vas developed with nutrient additive, stabilizing agent and mineral materix. For preparation of microbial agent, developing of formulation technique was very helpful for incresing the cell survival rate.

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