• Journal of Bio-Environment Control
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• v.9 no.3
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• pp.179-184
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• 2000

This study was conducted to improve control system of insect pests, especially western flower thrips, Frankliniella occidentalis, and two-spotted spider mites, Tetreanychus urticae, of rose greenhouses. Density of thrips was relatively higher in yellow flowers than in pink or redflowers, while there was no difference in density of two-spotted spider mites by flower colors. In pest control by an automatic monorail sprayer, 89% labor and 18.2% chemical savings, as compared to the conventional high pressure spray method, were achieved without lowering the pest control effectiveness. By using an adhesive agent in combination with acaricide, adhesion of chemical to crops increased by 25% and control effect on two-spotted spider mites increased by 20.5% as compared acaricide spray alone.

• The Korean Journal of Pesticide Science
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• v.11 no.1
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• pp.32-37
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• 2007

The study was carried out to establish buffer zone for the protection of the non-target organisms by aerial pesticide application. The two pesticide combination of 3-way tank-mixing of three pesticides for aerial application, ferimzone tricyclazole SC + BPMC EC + validamycin-A SL and hexaconzole EC + isoprothiolane EC+phenthoate EC were selected for the simultaneous control of key pests on paddy rice as blast, sheath blight, brown planthopper and moth. Aquatic organisms including killifish and loach in the paddy field and nearby water reservoirs were not affected by aerial application of the pesticides. However, all the water flea were killed, when they were exposed 10 m from the aerially sprayed site, while the water flea exposed in 30 m away from the site were not affected. Honeybees within 50 m in the wind direction and 20 m in the opposite wind direction showed a mortality of $7{\sim}100%$. Residues concentration of the pesticides in paddy water were not detectable level after six days from aerial application. Drifting distance of aerially sprayed droplet from the target area was within 30 m in the wind direction and 20 m in the opposite direction. Consequently, it was the buffer zones in the aerial pesticides application for the protection of the non-target organisms should be at least 50 m for aquatic organisms and 100 m for honeybees.

• The Korean Journal of Pesticide Science
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• v.20 no.3
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• pp.181-188
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• 2016

BtPlus is a group of biopesticides that are made of Bacillus thuringiensis and immunosuppressant. A new BtPlus that exhibits high insecticidal activity against mosquito larvae has been investigated in control efficacy in field conditions and its environmental safety against aquatic system. This study assessed the control efficacy of BtPlus against mosquito larvae with two different application methods. In aerial spraying application (100 mL per $3.3m^2$), BtPlus was effective at 50% or above formulation concentrations to control mosquito larvae. For a direct application to aqueous mosquito habitat, a semi-field mimicking paddy rice field was constructed. In this condition, BtPlus showed 80% and 100% control efficacies at 0.1% and 0.2% concentrations, respectively. BtPlus also showed 40% mortality against adults at 0.1% concentration in 10% sugar bait. However, its control efficacies against adults were much less than against larvae. Safety assessment of BtPlus against ecosystem was evaluated using young carp (Cyprinus carpio), a water flea (Daphnia magna), and a honey bee (Apis mellifera). BtPlus did not give any adverse effects on these nontarget organisms. Based on these results, BtPlus can be applied to control mosquitoes by direct aqueous application to paddy rice field.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.10 no.3 s.90
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• pp.34-41
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• 1989

최근에 이르러 농약의 살포량 증가에 따른 여러가지 부작용이 대두되므로 무엇보다 살충제를 적게 살포하면서 해충방제의 효력을 경제적 수준 이상으로 높여보자는데 천적의 역할을 평가하지 않을 수 없게 되는듯 싶다. 따라서 작물 해충에 대한 천적들의 종류와 분포상황, 기존 천적들을 보호 증식하는 문제 또는 병원 미생물 천적을 배양하며 농약과 함께 살포하는 문제 등 다양한 연구가 활발히 진행중에 있다고 본다. 그러나 살충제를 살포하지 않고 순수 생물적 방제 수단으로 작물을 재배할 수 있다면 더없이 이상적인 작물재배가 되겠으나 인구 증가에 따른 식량 증산은 필수적이므로 해충방제에 있어서 천적과 화학적 살충제를 동시에 적용하여 방제할 수 있는 종합방제가 바람직할 것으로 생각되어 간략히 소개해본다.

• Korean journal of applied entomology
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• v.47 no.4
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• pp.435-445
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• 2008

Due to internal feeding behavior, the oriental tobacco budworm, Helicoverpa assulta ($Guen\acute{e}e$), infesting hot pepper has been regarded to be effectively controlled by targeting egg and neonate larval stages just before entering the fruits. This study aimed to develop an efficient biological control method focusing on these susceptible stages of H. assulta. An egg parasitoid wasp, Trichogramma evanescens Westwood, was confirmed to parasitize the eggs of H. assulta. A mixture of Gram-positive soil bacterium, Bacillus thuringiensis subsp. kurstaki, and Gram-negative entomopathogenic bacterium, Xenorhabdus nematophila ANU101, could effectively kill neonate larvae of H. assulta. A sex pheromone trap monitored the occurrence of field H. assulta adults. The microbial insecticide mixture was proved to give no detrimental effects on immature development and adult survival of the wasp by both feeding and contact toxicity tests. A combined treatment of egg parasitoid and microbial pesticide was applied to hot pepper fields infested by H. assulta. The mixture treatment of both biological control agents significantly decreased the fruit damage, which was comparable to the chemical insecticide treatment, though either single biological control agent did not show any significant control efficacy. This study also provides morphological and genetic characters of T. evanescens.

• Asian Journal of Turfgrass Science
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• v.20 no.2
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• pp.175-190
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• 2006

The term mycoherbicide started in 1970, but its interest heightened due to increase costs of chemical herbicides. A classical biocontrol agent is expected to become a permanent part of its new environment and do no harm to it. Contemporary biological control agent(BCA) must be produced by artificial culture and could be applied like chemical herbicides over weeds. BCA is different from the classical approach in that it released through natural spread. To date 26 species of fungi are used as classical BCA against 26 species of weeds in seven countries. There are a number of examples of pathogens attacking non-target plants. But through risk assessments which include understanding the taxonomy, biology and ecology, the target and non-target species, it will be safe to introduce of exotic pathogens to control weeds. But pathogens have not been successfully used in practice. Many mycoherbicides show potential in laboratories, but are ineffective in the field and not consistent from year to year or field to field. There is also a lack of understanding humidity, dew formation and temperature and their effects on suppression of weeds by plant pathogens. Potential pathogen must be selected as a BCA. Previous studies suggest that these pathogens must (1) produce abundant and durable inoculum in artifical culture, (2) be genetically stable and weed specific and (3) kill weeds in control. A granular preparation of mycoherbicide into sodium alginate is lighter than liquids and less bulky than organic matter. Gel forms have also been used.

• Landscaping Tree
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• s.129
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• pp.28-34
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• 2012

• Asian Journal of Turfgrass Science
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• v.20 no.2
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• pp.191-201
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• 2006

Dollar spot caused by Sclerotinia homeocarpa is one of major diseases in putting greens. Microorganisms antagonistic to S. homeocarpa, a pathogen of dollar spot, were primarily screened through in vitro tests, including dual culture method and triple layer agar diffusion method. In vivo tests were also conducted to select the best candidate for a biocontrol microorganism, using pot experiment. Bacillus subtilis EW42-1 and Trichoderma harziaum GBF-0208 were finally selected as biocontrol agents against dollar spot. Relative Performance Index(RPI) was used as a criterion of selecting potential biocontrol agents. B. subtilis EW42-1 and T. harzianum GBF-0208 showed resistance to several agrochemicals mainly used in a golf course. B. subtilis EW42-1 and T. harzianum GBF-0208 suppressed effectively the disease progress of dollar spot like synthetic fungicide tebuconazole in the nursery where dollar spot had seriously occurred. B. subtilis EW42-1 and T. harzianum GBF-0208 have a potential to be biocontrol agents for the control of dollar spot.

• Korean Journal of Heritage: History & Science
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• v.48 no.3
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• pp.138-153
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• 2015

For preventing the biological damage domestically, insect damage control method using chemical medicine has been applied. However, it is trend to avoid existing chemical medicine such as Methyl bromide because of the problem of being harmful to human body and environment. Therefore, the research for new medicine to replace this has been done and the interest to the physical treatment such as temperature(high & low) treatment, hypoxic treatment, carbon dioxide treatment, and radiation treatment has increased. However the systematic application standard and way has not been established. Therefore, in this research, we are going to organize present condition of domestic cultural asset preventing biological damage after the 1980's. It will also consider the direction through the characteristic of technology, limit, and replacement medicine by examining the past research.

• Microbiology and Biotechnology Letters
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• v.27 no.6
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• pp.427-432
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• 1999

For the development of a multifunctional biocontrol agent, the siderophore-producing strain GL7 was isolated from a rhizosphere on chrome azurol S agar. The GL7 was identified as a strain of Pseudomonas fluorescents on the basis of their reactions to standard physicochemcial tests from Bergey's manual, API diagnostic test, and fatty acid analysis. P. fluorescents GL7 considerably inhibited spore germination and hyphal growth of phytopathogenic fungus Funsarium solani in a dual culture. In pot trials of bean with P. fluorescens GL7, the disease incidence was significantly reduced down to 5% from 70% of incidence in the untreated control. P. fluorescens GL7 also enhanced plant growth to nearly 1.5 times than that of the untreated control, promoting elongation and development of the roots. These results suggest that the plant growth-promoting P. fluorescens GL7 can play an important role in the biological control of soil-borne plant disease in a rhizosphere.