• Journal of the Korean Geographical Society
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• v.44 no.3
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• pp.372-394
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• 2009

This study investigates the ontology-based public participation GIS(PPGIS). The major reason that ontology-based GIS has attracted attention in semantic communication in recent year is due to the wide availability of geographical variable and the imminent need for turning such recommendation into useful geographical knowledge. Therefore, this study has been focused on designing and implementing the pilot tested system for public participation GIS. The applicability of the pilot tested was validated through a simulation experiment for history tourism in Guri city Gyeongi-do, Focused on the methodology, the life cycle model which involves regional statues and user recognition, can be viewed as an important preprocessing step(specification, conceptualization, formalization, integration and implementation) for recommended geographical knowledge discovery by axiom. Focusing on practicality, ontology in this study would be recommended for geographical knowledge through reasoning. In addition, ontology-based public participation GIS would show integration epistemological and ontological approach, and be utilized as an index which is connected with semantic communication. The results of the pilot system was applied to the study area, which was a part of scenario. The model was carried out using axiom of logical constraint in the meaning of human-activity.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.10a
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• pp.335-338
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• 2017

Presently, the collaboration hardware system and software technology that promoted commercializing ICBMS for integrated system visibility evaluation. This variation will move on the next pervasive period that mixed with cultural and technology convergence. There is possibility for the period system can invoke unpredictable confusing blank state. The blank state systems have ecosystem characteristics that are supplied, maintained and operated through the complex interactions of technology and culture. Using universal middleware can support the life-cycle model and increase the visibility of complex systems and prepare for confusing situations. In this study, based on universal middleware, data and service dynamic standardized modules were evaluated to support stable system visibility platform. The system visibility module consists of Intelligent Pervasive Cloud module, Memorial Service module and Life Cycler connection module. each module reflects various requirements of system visibility requested by external system. In addition, the analysis results are supported by various network application service standards through platform independent system and architecture.

• Journal of Life Science
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• v.26 no.3
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• pp.376-386
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• 2016

Apoptosis induction has been proposed as an efficient mechanism by which malignant tumor cells can be removed following chemotherapy. The intrinsic mitochondria-dependent apoptotic pathway is frequently implicated in chemotherapy-induced tumor cell apoptosis. Since DNA-damaging agent (DDA)-induced apoptosis is mainly regulated by the tumor suppressor protein p53, and since more than half of clinical cancers possess inactive p53 mutants, microtubule-damaging agents (MDAs), of which apoptotic effect is mainly exerted via p53-independent routes, can be promising choice for cancer chemotherapy. Recently, we found that the apoptotic signaling pathway induced by MDAs (nocodazole, 17α-estradiol, or 2-methoxyestradiol) commonly proceeded through mitotic spindle defect-mediated prometaphase arrest, prolonged Cdk1 activation, and subsequent phosphorylation of Bcl-2, Mcl-1, and Bim in human acute leukemia Jurkat T cells. These microtubule damage-mediated alterations could render the cellular context susceptible to the onset of mitochondria-dependent apoptosis by triggering Bak activation, Δψm loss, and resultant caspase cascade activation. In contrast, when the MDA-induced Bak activation was inhibited by overexpression of anti-apoptotic Bcl-2 family proteins (Bcl-2 or Bcl-xL), the cells in prometaphase arrest failed to induce apoptosis, and instead underwent mitotic slippage and endoreduplication cycle, leading to formation of populations with 8N and 16N DNA content. These data indicate that cellular apoptogenic mechanism is critical for preventing polyploid formation following MDA treatment. Since the formation of polyploid cells, which are genetically unstable, may cause acquisition of therapy resistance and disease relapse, there is a growing interest in developing new combination chemotherapies to prevent polyploidization in tumors after MDA treatment.

• The KIPS Transactions:PartD
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• v.12D no.7 s.103
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• pp.947-956
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• 2005

Software(S/W) reengineering is one of the effective technologies to produce a business worth and en and the S/W ROI continuously. In spite of, S/W reengineering has been recognized a cost-consumptive works with inefficient productivity. In fact we have used to transform to confusion system with destructive system architecture by extending and updating legacy system in a temporary expedients. Moreover it is impossible to provide the time-market products for coping with rapid changeable system environment and meeting to complicated customer's requirements. Therefore, we need a systematic reengineering methodology to fulfill the changeable environment, as appearance of new IT techniques, various alteration of business information model, and increment of business logic. Legacy systems can be utilized as the core property in business organization through reengineering methodology. In this paper, we target to establish the reengineering process, proposed MaRMI-RE consisting of initial Planning phase, reverse engineering and component transformation phase. To describe the MaRMI-RE, we presented the concrete tasks and techniques and artifacts per individual phase in process, and the case study is showed briefly.

• Journal of Life Science
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• v.25 no.5
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• pp.607-614
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• 2015

Cancers are the largest cause of mortality and morbidity all over the world. Cordycepin, an adenosine analog, is a major functional component of the Cordyceps species, which has been widely used in traditional Oriental medicine. Over the last decade, this compound has been reported to possess many pharmacological properties, such as an ability to enhance immune function, as well as anti-inflammatory, antioxidant and anti-cancer effects. Recently, numerous studies have reported interesting properties of cordycepin as a chemopreventive agent as well. There is an accumulating body of experimental evidences suggesting that cordycepin impedes cancer progression by promoting apoptosis, inducing cell cycle arrest, modulating intracellular signaling pathways, and inhibiting invasion and metastasis of cancer cells. In many cancer cell lines, cordycepin inhibits growth and cell cycle progression by inducing arrest of the G2/M phase, resulting from the inhibition of retinoblastoma protein phosphorylation and induction of cyclin-dependent kinase inhibitors. To induce apoptosis, cordycepin activates the extrinsic and intrinsic pathways, which promotes reactive oxygen species generation and the downstream activation of kinase cascades. Cordycepin also can activate alternative pathways to cell death such autophagy. In addition, cordycepin can inhibit the pro-metastatic processes of cancer cell detachment, migration, and invasion through a variety of mechanisms, including the nuclear factor-kappa B and activated protein-1 signaling pathways. In this review, we summarized the variety of action mechanisms by which cordycepin may mediate chemopreventive effects on cancer and discussed the potential of this natural product as a promising therapeutic inhibitor of cancer development.

• Journal of Life Science
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• v.24 no.1
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• pp.92-97
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• 2014

Cordycepin, an active component originally isolated from the traditional medicine Cordyceps militaris, is a derivative of the nucleoside adenosine, which has been shown to possess a number of pharmacological properties, including antioxidant and anti-inflammatory activities, immunological stimulation, and antitumor effects. This study was conducted on cultured human prostate carcinoma LNCap cells to elucidate the possible mechanisms by which cordycepin exerts its anticancer activity, which, until now, has remained poorly understood. Cordycepin treatment of LNCap cells resulted in dose-dependent inhibition of cell growth and the induction of apoptotic cell death as detected by an MTT assay, cleavage of poly ADP-ribose polymerase, and annexin V-FITC staining. Flow cytometric analysis revealed that cordycepin resulted in G2/M arrest in cell cycle progression and downregulation of cyclin B1 and cyclin A expression in a concentration-dependent manner. Moreover, the incubation of cells with cordycepin caused a striking induction in the expression of the cyclin-dependent kinase (CDK) inhibitor p21Waf1/Cip1 without affecting the expression of the tumor suppressor p53. It also resulted in a significant increase in the binding of CDK2 and CDC2 to p21. These findings suggest that cordycepin-induced G2/M arrest and apoptosis in human prostate carcinoma cells is mediated through p53-independent upregulation of the CDK inhibitor p21.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.1019-1022
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• 2007

Thrombolytic therapy with tissue plasminogen activator (tPA) can improve the clinical outcome of ischemic stroke patients. However, its clinical application is limited by narrow therapeutic time windows and elevated risks of cerebral hemorrhage and brain injury. In part, these effects of tPA has been related to matrix metalloproteinase-9 (MMP-9) dysregulation. Here, we investigate that the effects of alteplase (tPA with short half-life) and pamiteplase (a modified tPA with long half-life) on the MMP-9 regulation in neurovascualr unit. The total levels of MMP-2 and MMP-9 in neuronal cells are lower than astrocytes. Alteplase (1-10 ${\mu}g/ml$) induced upregulation of MMP-2 and MMP-9 in rat cortical neurons and astrocytes, respectively. Whereas pamiteplase in a wide range of dose did not affect the MMP-2 and MMP-9 responses in both of cells. These results suggest that pamiteplase with long half-life can be provided as a agent that overcome the side effects of alteplase.

• Proceedings of the Korean DIstribution Association Conference
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• 2003.02a
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• pp.169-200
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• 2003

소비자의 욕구변화와 정보기술의 발전으로 정보경제시대, 신경제시대가 도래함에 따라 가치사슬이 변화하고, 새로운 가치를 창출하는 신산업이 등장하고 있다. 인터넷의 확산과 더불어 선으로부터의 자유, 개인성, 즉시성이 가능한 모바일 비즈니스가 우리나라에서도 태동하고 있다. 모바일 비즈니스는 금융업체, 정보제공업체, 통신망 운용업체, 최종이용자간의 새로운 가치의 결합이 특징이며, 막 흐름이 만들어지고 발전되고 있는 상황이다. 이와 같이 다양한 이해관계자가개입하고 있어 협력이 특히 강조되고 또 요구되고 있다. 이러한 중요성에도 불구하고 이들간의 관계에 대한 연구가 미흡한 편이다. 따라서 본 연구는 모바일 비즈니스를 주도하고 있는 통신망 제공업체와 모바일 컨텐츠 공급 파트너간의 조직간 협력관계에 대한 실증 연구를 수행하였다. 관계마케팅, 거래비용, 자원기반관점, 네트워크 이론 등 기업간 관계를 기술, 설명, 예측하는 주요이론에서 논의되고 있는 개념을 바탕으로 본 연구는 컨텐츠 공급파트너의 전략적 성과에 영향을 주는 연구 모형을 제시한다. 최근 중요성이 더 강조되고 있는 항목은 신뢰와 결속임을 감안하여, 매개변수로써 전략적 성과에 영향을 주는 주요한 요소로 신뢰와 결속을 제시하고 있다. 컨텐츠 제공업체의 전략적 성과는 서비스제공업체에 대한 신뢰와 결속에 큰 영향을 받고있다는 점을 반영하고 있다. 한편 신뢰에 영향을 주는 독립변수로는 서비스 제공업체에 대한 평판, 서비스제공업체와 컨텐츠 제공 파트너간의 상호의사소통, 그리고 업무처리의 절차에 대한 공정성을 제시하고 있다 결속에 영향을 미치는 독립변수로는 동의된 목표, 업무정보교류, 거래특유자산의 3개의 변수를 선정하였다. 독립, 매개, 종속변수간의 9개의 연구 가설을 수립하였다. 국내의 주도적인 이동통신망소유업체에 모바일 컨텐츠를 공급하고 있는 170여개의 파트너를 대상으로 실증연구를 수행했다. 공변량 구조분석을 위주로 분석한 연구의 결과에 의하면 본 연구의 모델은 충분한 적합성을 보여주고 있는 것으로 나타났다. 또한 가설의 상당수가 채택이 되었다. 그러나 모바일 비즈니스의 기업간관계의 실제측면을 나타내는 업무정보교류와 결속자의 관계의 유의하지 않게 나와 기각되었다. 기각에 대한 여러 가지의 해석이 가능하지만 측정의 신뢰성을 확보해야 하고 개념의 타당성을 더 엄격하게 준수할 필요성이 제기되었다. 본 연구는 조직이론에 근거한 변수를 채택하고 있으나 모바일 비즈니스의 생명주기특성상 태동기이기 때문에 기업간 관계를 연구하는 측면에서는 탐험적 연구성격이 강하다. 더 나아가 본 산업의 주된 연구가 질적이고 기업내부만을 연구했던 것에 비교하면 시초적이라고 할 수 있다. 또한 관계마케팅, CRM 등의 이론적 배경이 되고 있는 신뢰와 결속의 중요성이 재확인하는 결과도 의의라고 할 수 있다. 그리고 신뢰는 양사 간의 상호관계에서 조성될 수 있는 특성을 가진 반면, 결속은 계약관계 초기단계에서 성문화하고 규정화 할 수 있는 변수의 성격이 강하다고 할 수가 있다. 본 연구는 복잡한 기업간 관계를 지나치게 협력적 측면에서만 규명했기 때문에 많은 측면을 간과할 가능성이 있다. 또한 방법론적으로 일방향의 시각만을 고려했고, 횡단적 조사를 통하고 국내의 한 서비스제공업체와 관련이 있는 컨텐츠 공급파트너만의 시각을 검증했기 때문에 해석에서 유의할 필요가 있다. 또한 타당성확보 노력을 기하였지만 측정도구 면에서 엄격한 개발과정을 준수하지는 못했다. 향후에는 모바일 컨텐츠 파트너의 기업의 특성을 조사하여 관계성 변수와의 상호관련연구를 진행할 필요가 있다. 관계기간, 의존성, 거래처의 단/복수여부, 서비스 범주 등의 제반 변수를 고려하여 이러한 변수가 양사와의 관계성 변수에 어떤 영향이 있는가를 검증할 필요가 있다. 또한 신뢰, 결속 등 다차원의 개념에 대한 심도 깊은 연구와 최근 제기되고 있는 이론의 확대도 필요하다. 마지막으로 신뢰와 결속에 영향을 미치는 요소간의 개념적 분류, 차이의 검증, 영향력 등을 광범위하게 진행시킬 필요가 있다.

• Journal of Life Science
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• v.33 no.12
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• pp.1002-1014
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• 2023

The root of Cirsium japonicum var. maackii (Maxim.) has long been used in traditional medicine to prevent the onset and progression of various diseases and has been reported to exert a wide range of physiological effects, including antioxidant activity. However, research on its effects on hepatocytes remains scarce. This study used the human hepatocellular carcinoma HepG2 cell line to investigate the antioxidant activity of ethanol extract of C. japonicum root (EECJ) on hepatocytes. Hydrogen peroxide (H₂O₂) was used to mimic oxidative stress. The results showed that EECJ significantly reverted the decrease in cell viability and suppressed the release of lactate dehydrogenase in HepG2 cells treated with H₂O₂. Moreover, an analysis of changes in cell morphology, flow cytometry, and microtubule-associated protein light chain 3 (LC3) expression showed that EECJ significantly inhibited HepG2 cell autophagy induced by H₂O₂. Furthermore, it attenuated H₂O₂-induced apoptosis and cell cycle disruption by blocking intracellular reactive oxygen species and mitochondrial superoxide production, indicating strong antioxidant activity. EECJ also restored the decreased levels of intracellular glutathione (GSH) and enhanced the expression and activity of superoxide dismutase and GSH peroxidase in H₂O₂-treated HepG2 cells. Although an analysis of the components contained in EECJ and in vivo validation using animal models are needed, these findings indicate that EECJ is a promising candidate for the prevention and treatment of oxidative stress-induced liver cell damage.

• Research in Plant Disease
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• v.28 no.3
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• pp.113-121
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• 2022

Rice blast is a major plant disease that occurs worldwide and significantly reduces rice yields. Rice blast disease occurs periodically in Korea, causing significant socio-economic damage due to the unique status of rice as a major staple crop. A disease outbreak prediction system is required for preventing rice blast disease. Epidemiological investigations of disease outbreaks can aid in decision-making for plant disease management. Currently, plant disease prediction and epidemiological investigations are mainly based on quantitatively measurable, structured data such as crop growth and damage, weather, and other environmental factors. On the other hand, text data related to the occurrence of plant diseases are accumulated along with the structured data. However, epidemiological investigations using these unstructured data have not been conducted. The useful information extracted using unstructured data can be used for more effective plant disease management. This study analyzed news articles related to the rice blast disease through text mining to investigate the years and provinces where rice blast disease occurred most in Korea. Moreover, the average temperature, total precipitation, sunshine hours, and supplied rice varieties in the regions were also analyzed. Through these data, it was estimated that the primary causes of the nationwide outbreak in 2020 and the major outbreak in Jeonbuk region in 2021 were meteorological factors. These results obtained through text mining can be combined with deep learning technology to be used as a tool to investigate the epidemiology of rice blast disease in the future.