• Journal of Life Science
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• v.19 no.1
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• pp.147-151
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• 2009

D-pinitol, another chemical structure of 3-O-methyl-D-chiro-inositol, is an important insulin-sensitizer. The purpose of this review is to examine the characteristics of pinitol and other analogs as functional food biomaterials which were well known to reduce blood glucose levels. Pinitol can be converted to chiro-inositol in normal humans, while diabetic patients can not use the molecule, resulting in exhibiting low level of chiro-inositol in their urine. Recently, it is reported that pinitol can trigger phospholipase C/D, thus the rate of glucose metabolism accelerates to use as fuel for human body. To not only reduce insulin resistance of diabetic patients, but also alleviate the symptoms of diabetes, obesity, and muscle contraction, pinitol and its dietary supplementation is needed.

• Journal of Life Science
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• v.8 no.1
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• pp.67-71
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• 1998

In order to develop new antitumor agents from fermentation broths, we used cccDNA breakage assay abd sulforhodamine B assay for prescreening. As a result, it was shown that sample reach 3.3% when using cccDNA breakage assay. In sulforhodamine B assay, we obtained 4 acive fraction against A549 (a cell line of human lung carcinoma) and SK-OV-3 (a cell line of human adenocarcinoma). These results suggest that these assay would be a promising method for antitumor prescreening from microbial sources.

• Microbiology and Biotechnology Letters
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• v.21 no.2
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• pp.187-191
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• 1993

In order to find out an anticariogenic substance, antibacterial activities of some medical herbs and spices against Streptococcus mutans were investigated. The essential oils of oregano, thyme, sage, fennel, nutmeg, rosemary, calamus and cassia cortex were shown antibacterial effect against S. mutans. The essential oil of oregano among other tested medical herbs and spices showed the most active antimicrobial activity. The essential oil of oregano inhibited the activity of glucosyltransferase from S. mutans. The minimal inhibition concentration of oregano essential oil was determined as 0.05nl/ml. The activity of glucosyltransferase from S. mutans was inhibited in the presence of oregano essential oil.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.193-198
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• 2000

PCBs를 분해하는 bphABC유전자를 plasmid vactor pMFB2에 유전자조작한 Pseudomonas putida AC30(pMFB2)를 PCBs에 오염된 연안해역의 해수와 저니로 만든 microcosm에 도입한 결과, 각각 도입 4일과 7일만에 사멸하였다. 그러나, 도입한 P. putida AC30(pMFB2)는 사멸하였지만, 연안해수와 저니 microcosm에서 plasmid pMFB2가 전이한 토착미생물이 검출되었다. 도입한 P. putida AC30(pMFB2)의 생잔실패의 원인을 분석한 결과 공경 0.2$\mu\textrm{m}$의 filter를 통과하는 물질과 생물이 가장 크게 영향을 미치는 것으로 나타났다. 유전자조작 P. putida AC30(pMFB2)의 도입과 bphABC유전자의 토착미생물로의 전이에 따른 토착미생물군집에 미치는 영향을 개체수 변동으로 조사한 결과, 토착미생물 군집에 미치는 영향은 보이지 않았다. P. putida AC30(pMFB2)의 도입에 의한 PCBs의 생분해성을 분석하였다. 그러나, 도입한 유전자조작 균주가 생잔에 실패함으로써 잔류하고 있는 PCBs의 농도변화는 보이지 않았다.

• Microbiology and Biotechnology Letters
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• v.23 no.4
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• pp.439-445
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• 1995

Identification of Actinomycetes isolate strain SH-8002, a producer of ACE inhibitor, based on procedures employed in the international Streptomyces project. The strain, designated as SH-8002, was identified as Streptomyces zoamyceticus SH-8002 based on its morphological, physiological, biochemical and chemotaxonomic characteristics. The ACE inhibitor produced by the strain was highly achieved in fermentation medium condition that was 1% soluble starch, 0.5% tryptone, 0.2% K$_{2}$HPO$_{4}$, 0.2% CaCO$_{3}$, 0.1% NaCl, pH 8.0 at 30$\circ$C for 144 hrs.

• Microbiology and Biotechnology Letters
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• v.23 no.4
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• pp.397-404
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• 1995

Chemotaxonomy and numerical identification were carried out for an isolate strain No.8001 producing an antibiotic that generates oxygen radical. The genus of strain No.8001 was decided as Streptomyces sp. from chemotaxonomic data. Thirty-nine taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate was best matched to Streptomyces citreofluorescens in the major cluster 1B of Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces citreofluorescens.

• Journal of Life Science
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• v.13 no.3
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• pp.248-251
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• 2003

The kinetics of dimerization of a newly synthesized thyroglobulin (Tg), the precursor protein in the manufacture of thyroid hormone, was investigated in the endoplasmic reticulum of thyrocytes FRTL-5 cell line. The folded monomeric Tg was first detectable in a conformationally unstable form, from the examination of lysates of pulse labeled cultured thyrocytes by denaturing and nondenaturing gel electrophoresis by 15 min after biosynthesis. The first dimeric Tg was formed by 30 min after; the monomer declined and the dimer progressively increased, and 40 min after remarkable dimeric Tg form was found. Finally, dimerization was complete at 60 min after.

• Microbiology and Biotechnology Letters
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• v.28 no.5
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• pp.258-263
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• 2000

A fibrinolytic enzyme was purified to homogeneity from Bacillus sp. S19 using DEAE and CM column chromatograhies, and gel filtration with a recovery yield of 13%. Its molecular mass was estimated to be 42 kDa by SDS-PAGE. The pH and temperature optima were 8.0 and $40^{\circ}C$, respectively. The enzyme was stable up to $45^{\circ}C$ and over a pH range of 6-9. The N-terminal amino acid sequence of the enzyme was determined as Alsa-Gln-Asp-Ala-Thr-Val-Asn-Ile-Ser-Ala-Glu-Arg-Gln-Val-Ile. The fibrinolytic activity was increased by $Cu^{2+}$ while it was strongly inhibited by metal ions such as $Cd^{2+}$ and $Ba^{2+}$ . In addition, the enzyme was inhibited by EDTA, but not by PMSF, suggesting that it is a metallorprotease.

• Journal of Life Science
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• v.7 no.3
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• pp.186-191
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• 1997

In order to select new anticandiosis agent-producing candidates, we used a modified selection method. Two active fractions designated as S-6279A) and (B) were isolated from the fermentation broth of Streptomycetes sp. S627 which was screened by methanol extraction, diaion HP-20 and silica gel column chromatography (chloroform-me-thanol, and benzene-ethyl acetate), and preparative thin layer chromatography.

• Journal of Life Science
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• v.7 no.3
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• pp.180-185
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• 1997

Methylelaiophylin generated superoxide radicals in Bacillus subtilis and showed antibacterial activity against a broad range of gram positive bacteria. The inhibition of DNA synthesis was more sensitive than one of RNA synthesis. A recombination-deficient mutant strain of B. subtilis was 2-fold more sensitive than a wild strain, and this sensitivity was reduced in the presence of an antioxidant, dithiothreitol. Methylelaiophylin generated superoxide radicals in B. subtilis lysates, and this suggests that the antibacterial activity of methylelaiophylin is related to the generation of active oxygen species in the cells.