• Applied Biological Chemistry
• /
• v.13 no.3
• /
• pp.237-242
• /
• 1970

Fifty strains of mold which isolated from the various sources were screened for the production of Naringinase which hydrolyse naringin, the 7-rhamnoe-glucoside of 4'.5.7. - trihydroxyflavanonin, the main bitter principle of citrus fruits and grape fruits. Of the 4 strains yielded naringinase with significant activity, S-1 strain was selected on the criterion of industrial application, and some properties of crude naringinase of this S-1 was investigated. The results obtained were as follows. 1. Naringenase obtained from S-1 strain has optimum pH range from 3.0 to 5.0 for its activity. 2. Production of naringinase was increased on the addition of naringin to the medium. 3. Hydrolysis of naringin with approporiate concentration of naringinase was carried out linerly up to 80% on the 0.1% substrate solution. 4. The optimum temperature for its activity was $50^{\circ}C$, and this enzyme was inactivated 80% of its total activity at $70^{\circ}C$ for 10 minutes, 40% at $60^{\circ}C$ for 30 minutes. But signifiant decrease of activity were not occurred by heat treatment at $50^{\circ}C$ for 2 hours. 5. Crude enzyme of the naringinase obtained from S-1 strain was competitively inhibited by addition of glucose on the substrate, and inhibitor constant of the glucose on the this enzyme was 1.5 Mol, and inhibition rate were linearly increased according to the increase of sucrose concentration and 56% of its total activity was inhibited at 1 Mol sucrose solution.

• Applied Biological Chemistry
• /
• v.25 no.3
• /
• pp.189-196
• /
• 1982

Seventy two samples of feedstuff were collected from commercial channels all over Korea. As a study on the moisture contents, microflora and mycotoxin production of each sample investigated. Moisture content of the samples was $11.2{\sim}15%$. Total counts of the samples were $1.8{\times}10^{2}{\sim}2.4{\times}10^{6}$ per gram. The Coli-form group were counted from 9 to $6.3{\times}10^{5}$ per gram which composed mainly of Enterobacter and Klebsiella, whereas Escherichia coli was minor ones. The contamination of many feeds was not remarkable, and only some broiler feeds was contaminated largely with Escherchia coli. Fungi were below detectable limit in 45% of the samples and the most contaminated sample had $4.5imes}10^{5}/g fungi counts.

• Journal of Service Research and Studies
• /
• v.10 no.1
• /
• pp.1-15
• /
• 2020

This study was performed to derive a new business administration framework based on the service philosophy established in the previous research. We proposed a business administration system that reflects the changes of modern economic society and solves problems of existing business administration. The problem of the existing business administration is analyzed by analyzing the essential problems of business administration as a discipline and the practical problems of current business administration. New management theory must have a status as an intrinsic discipline, so it must meet the common principles of human society. The principles of universe and the life principle of mankind, which are the environment in which human beings live, were reflected. As a result of deriving these common principles, qualification requirements as the intrinsic disciplines of business administration can be defined. The new business administration discipline is constructed in three fields. Business philosophy, a theory of manager, and business administration skills are three sub-fields of new business administration. We define the detailed structure of each field of new business administration and present the main research topics. In the future, further research is needed to deepen the culture of essence in business administration, and it is necessary to study the construction of the new business administration theory in detailed field.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.22 no.1
• /
• pp.257-266
• /
• 2021

Urban areas can often suffer flood damage because of the more frequent catastrophic rainfall events from climate change. Flood mitigation measures consist of (1) structural and (2) non-structural measures. In this study, the proposed method focused on operating an urban drainage system among non-structural measures. The combined inland-river operation technique estimates the inflow of pump stations based on the water level obtained from a preselected monitoring point, and the pump station expels the stored rainwater to the riverside based on those estimates. In this study, the proposed method was applied to the Mokgam drainage watershed, where catastrophic rainfall events occurred (i.e., 2010- and 2011-years), and severe flood damage was recorded in Seoul. Using the proposed method, the efficiency of flood reduction from the two rainfall events was reduced by 34.9 % and 54.4 %, respectively, compared to the current operation method. Thus, the proposed method can minimize the flood damage in the Mokgam drainage watershed by reserving the additional storage space of a reservoir. In addition, flooding from catastrophic rainfall can be prevented, and citizens' lives and property in urban areas can be protected.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2021.06a
• /
• pp.282-282
• /
• 2021

강우시 농경지와 축산시설로부터 유출되는 비점오염물질은 하류 수계의 수질과 수생태계에 악영향을 미친다. 이에 따라 환경부에서는 비점오염원관리지역을 지정하고 다양한 비점오염 저감 대책을 시행하고 있다. 본 연구에서는 비점오염원관리지역으로 지정된 안동댐 하류 중 송야천 유역을 대상으로 강우유출수 모니터링을 수행하였으며, 모니터링 결과를 바탕으로 강우시 비점오염물질 유출 특성을 분석하였다. 모니터링 기간은 2020년 6월부터 11월까지 총 5회의 강우사상에 대하여 상·하류와 유입하천을 포함한 총 8개의 모니터링 지점을 대상으로 강우사상별 유량가중평균농도(Event Mean Concentration, EMC), 오염부하, 단위면적당 오염부하를 산정하였으며, 오염원 그룹별 비점배출부하를 산정하여 오염 기여도를 분석하였다. 강우유출수 조사결과를 이용한 EMC 농도 산정 결과 유입하천인 오산천 지점이 SS와 TOC 항목을 제외한 모든 수질항목의 EMC 농도가 가장 큰 것으로 나타났다. 단위면적당 오염부하를 산정하여 비교 분석한 결과 T-P 항목의 단위면적당 오염부하는 물한천 지점(0.69 kg/ha)과 오산천 지점(0.69 kg/ha)이 크게 나타났다. 결과와 같이 오산천 지점과 물한천 지점이 오염정도가 큰 것으로 나타났으며, 이에 따른 상류 오염원 현장 정밀조사를 수행하였다. 조사 결과 강우발생시 상류에 위치한 농경지와 축사에서 발생하는 오염원이 하천으로 유입되고 있었으며, 여러 축사에서 배출되고 있는 유입수를 채취하여 분석한 결과 T-P 농도가 평균 0.935 mg/L로 높게 나타났다. 전국오염원조사자료(국립환경과학원, 2017) 내용을 참조하여 송야천 유역의 오염원 그룹별 비점배출부하를 산정해 오염 기여도를 분석한 결과, T-P 항목의 경우 축산계와 토지계의 비점배출부하가 전체 비점배출부하의 약 63%와 37%를 차지해 비점배출부하 기여도가 큰 것으로 나타났다. 이와 같이 송야천 유역의 경우 강우시 농경지와 축산시설에서 배출되는 오염물질이 하천 수질오염에 상당한 기여를 하고 있는 것으로 보여지며, 비점오염원 발생에 대한 대책 마련이 필요할 것으로 사료된다. 본 연구 결과는 송야천 유역의 비점오염 저감 대책 수립을 위한 기초자료로 활용할 수 있을 것으로 판단된다.

• Journal of Ethics
• /
• no.80
• /
• pp.1-30
• /
• 2011

Confucianism and Buddhism were compatible in the period of Goryeo Dynasty. And then, the rulers worshiped Confucianism and repressed the religious activity of the Buddhist monk in the early years of the Joseon Dynasty. But King Sejong planed to bring reconciliation between Confucianism and Buddhism. Especially on the ceremonies of mourning and sacrificial rituals, he always performed the two religious style. The reason of sticking to his amicable consciousness is as follows. Firstly, he thinks that both the theory of Confucianism and the religious spirit of Buddhism are very important for the building up the foundation of his Dynasty. Secondly, his mind has been of a same faith cure on the incurable disease. Therefor, when the Royal family is taken ill he must pray to Buddha for recovery from the first stage. Thirdly, he regards social conditions to be the most important and respect for man's life and dignity for the purpose of beneficent administration. But Joseon's government line was the anti-Buddhist policy. So, the policy came in the wake of a debate among King Sejong and Confucian government officials. However his harmonious mind was unchanged between Confucianism and Buddhism. After all, in the last phase of his life he was deeply religious on Buddhism. I think that King Sejong's amicable consciousness could make a contribution to overcome religious conflicts and to create a new political cultural form in the modern society

• Journal of Hospice and Palliative Care
• /
• v.16 no.3
• /
• pp.155-165
• /
• 2013

Koreans' traditional view on death has been much influenced by Confucianism, Taoism, Buddhism, and shamanism since ancient times. Confucianism emphasizes the importance of the real life in this world and highly praises doing good deeds for the family and the community. It also praises people who are enlightened by education and self-discipline. Confucian scholars admit that death cannot be understood by rational thinking although it is unavoidable as a cosmic order. Taoism sees life as the same entity as death; Both are two different aspects of the same cosmos or the wholeness. However, the disciples of Taoism became much interested in a long life and well being that may be achieved by harmonizing with the cosmic order. Buddhism thinks that death and life are an "illusion". It says that people can be enlightened by recognizing the fact that "Nothing is born and nothing is dying in this world. Everything is the product of your mind occupied with false belief." However, secular Buddhists believe in the afterlife and metempsychosis of the soul. This belief is sometimes connected with the view of the traditional shamanism. Shamanism dichotomizes the world between "this world" and "that world". After death, the person's soul travels to "that world", where it may influence life of people who reside in "this world". And shamans who are spiritual beings living in "this world" mediate souls and living people. In conclusion, there are various views and beliefs regarding death, which are influenced by a number of religions and philosophies. They should be seriously considered when making a medical decision regarding the end of patients' life.

• Applied Biological Chemistry
• /
• v.47 no.1
• /
• pp.49-54
• /
• 2004

Biotransformation process using microorganisms was examined to improve the bioconversion rate for the production of sotolon from the raw material. First, the extraction condition was optimized with regard to solvent type and pretreatment conditions. Dichloromethane was selected as a suitable solvent for the extraction of sotolon and sotolon-related compounds. Second, various microorganisms such as lactic acid-producing bacteria, yeast and fungi were tested for the biotransformation. Among the tested microbes, Agaricus blazei showed the highest conversion rate. Additives including amino acids, salts, and organic acids were investigated to test their effects on bioconversion. When the solution was added by isoleucine, ${\alpha}-ketoglutaric\;acid$, ascorbate, and $FeSO_4$ and later incubated by culture broth containing the mycelium of Agaricus blazei, the sotolon content increased up to about 77 times as compared to that of the raw material.

• Proceedings of the Korea Contents Association Conference
• /
• 2009.05a
• /
• pp.559-566
• /
• 2009

The Capital punishment is a sentence which imposes the defendant to be put to death for his crime, thus depriving him/her of life and it's the heaviest punishment of all. It is a very sensitive issue in the sense that it denies a person's life and dignity. It is also an on-going issue which continues to cause political and ethical controversies. Although there is no direct stipulation acknowledging capital punishment in the constitution, there is controversy on the existence of indirect basis. The Art. 12. I. can not be regarded as an indirect provision if it is interpreted to have criminal punishments stipulated by written law. As the supreme Court and the Constitution Court are supporting the capital punishment which seems unconstitutional, legislative examination is inevitable. Considering the fact that the Constitution is neither for nor against the death penalty positively, it is possible to abolish it by enactment of a law and thus, constitutional amendment would not be needed.

• Korean Journal of Plant Tissue Culture
• /
• v.28 no.6
• /
• pp.335-339
• /
• 2001

Grapevine leafroll-associated 3 closterovirus (GLRaV-3) is phloem limited virus, and one of the most severe viral diseases found in Korea. However, nonhomogenous distribution and low concentration and seasonal variations of GLRaV-3 in grapevines remain as main problems which prevent the introduction and molecular biology or serology experiments. Virus-infected plantlet in vitro was obtained from node tissue cuttings, which was GLRaV-3 infected 'kyoho' vines. The amount of purified virus was highest in vitro plantlet. Moreover, viruses seem to be relatively homogeneously distributed in all organs including leaf, stem and callus derived from in vitro plantlets. RT-PCR detection using in vitro plantlet tissue as template was most effective. When comparing ELISA to RT-PCR, RT-PCR detection was 1,000 times as effective as ELISA. These results can be explained by improved quality such as tenderness or less tannins in plantlet in vitro. In conclusion, until infected herbaceous host will be available, tissue culture can be usefully adopted as a technique for a good source of GLRaV-3 closterovirus for further studies.