• Journal of Haehwa Medicine
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• v.4 no.2
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• pp.237-253
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• 1996

• The Microorganisms and Industry
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• v.15 no.2
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• pp.33-39
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• 1989

본고에서는 주로 방선균의 선택분리 과정을 시료(substrate)선정, 시료 전처리(pretreatment), 배지, 배양조건, 콜로니 선정 등으로 나누어 살펴보고자 한다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.65-65
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• 2018

복분자(Rubus coreanus Miquel)는 anthocyanin, phenolic acid, tannin, flavonoid, stilbenoid와 같은 생리활성물질이 풍부하게 존재하는 것으로 보고되었으며 항염증, 항암활성, 항산화효과 등 다양한 생리활성에 대한 효능이 있어 건강식품으로써 애용되고 있을 뿐만 아니라 약용성 건강보조식품 등으로 각광받고 있다. 생물전환(Bioconversion)은 미생물 또는 효소의 생물학적 촉매 기반 천연소재의 생리활성 물질기능성, 생체이용률, 안전성을 증대시키기 위한 방안으로 많은 연구가 진행되고 있으며 아울러 식품, 의약품, 화장품 등 다양한 분야에서 활성화 되고 있다. 본 연구는 불가사리 발효액으로부터 유산균을 분리하였으며, 유전학적 특성을 확인하기 위하여 16S rDNA 염기서열을 분석하였다. 전북 고창에서 수확된 복분자를 분말상태로 유산균과 발효공정을 수행하였으며, 복분자의 최적 추출조건 선정과 발효공정 전 후의 활성을 관찰하였다. 발효공정 후 추출물의 기능성 평가를 진행하기 위하여 DPPH radical scavenging activity, total polyphenol 함량을 확인하여 항산화 효능 및 유효성분 함량을 평가하였다. 또한 대식세포인 Raw 264.7을 사용하여 MTT assay, Nitric oxide (NO) 생성 억제 효능을 확인하여 세포독성 및 항염증 활성을 평가하였다. 실험결과, 발효기간이 다른 불가사리 비료발효액으로부터 16 종의 다양한 균주를 확보하였으며, 생물전환 공정에 유용 균주를 선정하기 위하여 복분자 분말의 발효공정을 실시한 결과 3 종의 유산균 처리군에서 무처리군 대비 DPPH radical 소거능 및 polyphenol 함량이 증가됨을 확인하였다. 그 중 가장 우수한 활성을 나타내는 균주를 16S rDNA 염기서열 분석한 결과 Lactobacillus coryniformis A6-4로 확인되었으며, 발효공정 후 항산화 활성은 무처리군 대비 약 115%, polyphenol의 함량은 무처리군 대비 약 121%로 증가됨을 확인되었다. 또한 발효공정 후 독성활성이 감소되는 경향을 확인되었으며, 항염증 활성이 유의적으로 증가됨을 확인하였다.

• Microbiology and Biotechnology Letters
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• v.17 no.4
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• pp.307-312
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• 1989

Strain No.77-AG-567 showed antifungal activity against Pyricularia oryzas and Sphaerotheca fuliginea. In the process of purification of active component this strain was found to produce blasticidin S together with another antibiotic. This compound was identified as being acetoxycyc-heximide, also referred to as E-73, by UV and $^1$H NMR spectral data. Identification of this strain led us to conclude that strain No.77-AG-567 is most probably be Streptomyces atratus. It showed different characteristics from S. atbutus, so far known to produce both blasticidin S and acetoxycycloheximide. Particularly worthy of note was the difference in spore surface. In addition, acetoxycycloheximide has been known to have activity only against yeast and tumor cells but we found that it also has activity against Pyricularia oryzae and Sphasrotheca fuliginea.

• Microbiology and Biotechnology Letters
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• v.10 no.3
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• pp.171-175
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• 1982

An oxytetracycline as being a tetracycline-antibiotic substance displayed a general synergism in the antimicrobial activity by the interaction of ginseng saponin and antibiotics, but did not to Sarcina maginata. Penicillin G.Na as being a $\beta$-lactam-antibiotic substance displayed a synergism in the antimicrobial activities by the addition of ginseng saponin to microorganisms used, but changed the effects in the antimicrobial activity of penicillin G.Na against the genus Serratia. An antimicrobial activity by the addition of ginseng saponin to antibiotics showed a non-specificity, and it varied synergism or antagonism to Gram-positive or Gram-negative bacterium. It was assumed that a drug-resistance could be eliminated by the dual administration of ginseng saponin and antibiotics.

• Journal of Bio-Environment Control
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• v.30 no.3
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• pp.218-229
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• 2021

Recently, as consumers' interest and importance in health care have significantly increased, they prefer natural and organic foods that do not use chemical pesticides. Since sprout vegetables effectively promote health and prevent diseases such as cancer and cardiovascular disease, the consumption of sprout vegetables, a highly functional and safe food, has been increased significantly. This study aimed to investigate the effect of light on the growth and bioactive compounds of seven different sprout vegetables. After sowing the seeds of various sprout vegetables (kale, Chinese kale, broccoli, red cabbage, alfalfa, red radish, and radish), the sprouts were cultivated under light conditions (20℃, RGB 6:1:3, 130 μmol·m^-2·s^-1, 12 hours photoperiod) and dark condition for 7 days. Sprouts samples were taken at 1-day intervals from 4 to 7 days after treatment. The fresh weight, dry weight, plant height, total phenol content, and antioxidant capacity were measured. Brassica species (kale, Chinese kale, broccoli, red cabbage) and Medicago species (alfalfa) had significantly higher fresh weight values under dark conditions, while the content of bioactive compounds was increased considerably under light conditions. In contrast, the fresh weight of Raphanus genus (red radish, radish) significantly increased under the light condition, but the antioxidant phenolic compounds were significantly higher under the dark state. A negative correlation was observed between the growth and secondary metabolites in various sprout vegetables. This study confirmed the effect of light and dark conditions on different sprout vegetables' growth and nutritional value and emphasizes the importance of harvest time in producing high-quality sprout vegetables.

• Microbiology and Biotechnology Letters
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• v.28 no.2
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• pp.97-104
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• 2000

Screening of Biologically Active Essential Oils from Ligusticum tenuissimum. Kim, Min-Hae, Young-Gil Kim, Jin-Ha Lee, Keo-Pyo Hong, Jung-Ki Hong, Young-Joon Kong, and Hyeon-Yong Lee*. Division of Food and Biotechnology, Kangwon National University, Chunchon 200-701, Korea, 1 Regional Crop Development Station, Kangwon Agricultural Research & Extension Services, Chunchon 200-150, Korea-The biological activities of the crude essential oils from Ligusticum tenuissimum and the control(phthalic anhydride) were compared. About 60% of the growth of MCF7, A549, and Rep3B cells were inhibited by adding 1.0 mg/ml of the crude essential oils and below 40% was observed by the control. Cytotoxicity on human normal lung cell(IMR90) was scored as 34.4% for the crude oil and 26.4% for control, respectively. It was found that the crude essential oils were more effective than the control in anti mutagenecity tested by both Rec-assay and CRG V79 cells. The growth of human T-cell(Jurkat) was enhanced up to 1.21 times by adding the crude essential oil compared with the control. 50% of a-glucosidase activity was inhibited by both the crude essential oil and the control. ACE activities were inhibited 80.1 % and 65.3% by adding 1.0 mg/ml of the crude oil and the control, respectively. The higher enhancement of glutathione-S-transferase activity was observed in the crude oil than those in the control: 301 % v.s 234% at 1.0 mg/ml of the treatment. Thrombolytic activity was measured as 42.9% and 28.6% for the crude oil and the standard, respectively. The effect of the oil on the nerve cells PCI2, was observed as follows: the neurite of PCl2 cells was lengthened up to 255 /-lm longer than 205 /-lm of control. The number of neurite-bearing cells were about two times higher than control. The survival ratio of the crude essential oil was also increased up to 56.4% which was about two fold higher than in control.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.3
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• pp.357-365
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• 2011

This study was conducted to compare biological activity of browning compounds from fresh, red and black garlic. Water soluble browning compounds were separated from fresh, red and black garlic by dialysis membrane. Antioxidation and other biological activities of freeze dried inner and outer parts from garlics were compared. pH of fresh and red garlic dialysis solutions were higher in inner part of membrane, but black garlic dialysis solution showed reverse tendency. Browning intensities of all tested samples were higher in outer part of dialysis solutions. In inner part dialysis solutions, contents of total phenol compounds were the lowest in red garlic while their contents were the highest in black garlic and thereafter, were the highest in inner part of dialysis solution made from red garlic. Flavonoids content was the highest in inner part of red garlic dialysis solution. Total pyruvate content was higher in outer part of fresh and red garlic dialysis solution which showed the opposite results in black garlic. Total thiosulfate content was the highest in black garlic, red garlic and fresh garlic in order. Antioxidant activities have some similarities among garlic products. DPPH radical scavenging activity was higher in inner part of fresh and black garlic and outer part of red garlic. Tyrosinase inhibition activity was higher in browning compounds of red garlic than fresh and black garlic. $\alpha$-glucosidase inhibition activity of tested samples were higher in inner part of dialysis solution than outer part, and showed higher activity in red garlic than fresh garlic at low sample concentrations.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.465-466
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• 2001

동물의 대사기능은 체내 주요 생리기능의 지표로서 체내의 물질대사 또는 에너지 대사의 결과를 반영한다 체내의 모든 생리적 과정은 동물과 환경간에 있어서 물질 및 에너지의 교환과 체내의 물질 및 에너지의 전환을 통해서 수행된다. 따라서 생체의 대사작용은 생존을 위한 역원적 중추기능이며 생체의 대사활성은 동물체의 기능적 항상성을 유지하는데 관련된 생리기능의 총체적 척도가 된다. (중략)

• Bulletin of Food Technology
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• v.23 no.3
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• pp.392-399
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• 2010

최근 초음파(ultrasound, US) 기술의 원리와 응용, 특히 식품의 생리활성물질에 관련된 응용기술에 대하여 기술하였다. 간단한 초음파 기술은 제외하고, 유화, 용해, 조직개선, 균질화(homogenization), 점도, 추출, 건조, 결정화, 거품제거(defoaming) 등의 기술에 관한 것이다. 특히 초음파에 의한 추출(UAE)을 상세히 기술하였다. 오늘날 초음파 기술은 생리활성물질의 추출 같은 산업화에 응용되고 있으며, 기능성을 강화하기 위한 추출에도 이용하고 있다. 이러한 초음파 기술은 장래 효과적인 식품기술이 될 것이며, 또한 식품의 성질을 이해하는 데 도움이 될 것이다.