• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.565-573
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• 2010

Thinning of apple fruitlets is one of the most laborious and important works for the improvement of fruit quality and for the promotion of sufficient flower bud formation to prevent alternate bearing in commercial cultivars. Lateral fruits of self-thinning apple cultivars fall naturally within 30 days after full bloom and only central fruit remains to mature. Differences of gene expression between central fruit and lateral fruit were investigated by differential display (DD) PCR. Partial cDNAs of 30 clones from the central fruit and 24 clones from the lateral fruit were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, senescence, temperature stress, protein degradation, fruit browning, sorbitol metabolism were significantly higher in pedicels of lateral fruit than in pedicels of central fruit. On the other hand, the up-regulation of proteins involved in anthocyanin and flavanol biosynthesis and ethylene synthesis were observed in pedicels of central fruit. In Real time PCR analysis, cytochrome P450 gene was confirmed as showing a higher expression level in lateral fruit than in central fruit. The results of this study indicate that differentially expressed genes are related to self-thinning characteristics in apple tree.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.2
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• pp.112-119
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• 2021

In order to develop a safe seeding technology for low-density transplantation, we analyzed the changes in seeding growth according to different seeding rates and seeding days. We detected changes in leaf number and shoot diameter on the 10th seeding day, and in plant height on the 20th seeding day according to seeding rate. The increase in shoot and leaf number, and shoot diameter per seeding day decreased with anincrease in seeding rate. On seeding day 20, the reductions in shoot and root dry weights according to seeding rate was lower compared with those recorded at seeding day 10. Shoot dry weight was significantly related to root dry weight, plant height, and shoot diameter. With an increase in seeding rate, seeding health decreased and there was an increase in the rate of seeding health per seeding day decreased. For low-density transplantation, the optimal number of seeds required for transplanting 3-5 plants per hill is 11,000, which is estimated to be approximately 280-300 g of seeds for 22 g brown rice. Furthermore, the appropriate number of seeding days is estimated to be 13 to 16 days, which corresponds to a small reduction in seeding health.

• Korean journal of applied entomology
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• v.52 no.4
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• pp.357-364
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• 2013

Bee-vectoring is a new crop protection technology used for suppressing insect pests and diseases in crops by disseminating microbial agents into plants during bee pollination activities. In this study, we conducted bee-vectoring trials in cherry tomato greenhouses by using the bumble bee (Bombus terrestris), a microbial agent (Bacillus subtilis) and a new dispenser, and we measured the delivered quantity of microbial agent. Bacterial colony forming units (CFUs) in bees exiting a dispenser ranged from $9.0{\times}10^5$ to $1.9{\times}10^6$ per bee. At greenhouse trials in the National Academy of Agricultural Science (NAAS) trials, 3,300 - 8,500 CFUs per flower were counted and 80 - 100% of the flower samples contained detectable concentrations. There was no significant difference in CFU density between microbial replacement intervals (once a week vs twice a week) in the NAAS trials. In a commercial greenhouse trial, 1,800 - 2,400 CFUs per flower were found, and 83 - 93% of the flower samples contained detectable concentrations. CFUs detected in bee-vectored flowers increased by approximately 75 times before bee-vectoring. The mortality of bumble bees in the NAAS trials was, on average, 22% and little negative effects were observed on the bumble bee colonies. The yield difference for cherry tomatoes in the NAAS trials was not significant between treatments. When we select additional microbial agents that can be disseminated using this technology and create a detailed plan based on insect pests and disease incidence, we can apply this technology in greenhouses for growing tomatoes and strawberries in the near future.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.4
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• pp.317-324
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• 2010

Recently, fallow paddy land located in the mid-mountain area (FPL) due to a decrease in rice consumption has increased in some regions of our country. So, grasses and forage crops available for years in once sowing should be introduced in FPL. This study was conducted to develop the technique for cultivation of grasses and forage crops using animal manure in FPL The field experiments were carried out from 2007 to 2009 on FPL at Kumsan, Chungbuk province in Korea. Cattle feedlot manure (CFM) was prepared by mixing feces and urine of cattle with rice straw in cattle feedlot for 6 months. The experimental plots were consisted of four treatments; tall fescue-based mixed pasture applied with chemical fertilizer (Control), mono-tall fescue pasture (MTFP), tall fescue-based mixed pasture (TFBMP), mono-Perennial ryegrass (MPR-FCM), mono-Italian ryegrass (MIR-FCM), mono-Red clover (MRC-FCM) and mono-Reed canarygrass (MRCG-FCM) applied with FCM. The field of tall fescue-based pasture had been sown with a grasses mixture containing 'Fawn' tall fescue, 'potomac' orchardgrass, 'Reveille' Perennial ryegrass, and 'kenblue' Kentuky bluegrass, 'Kenland' Red clover. Seeding rates were 16, 6, 4, 2 and $2\;ha^{-1}$, respectively. DM yields of forages and rates of grass coverage was higher in MTFP, TFBMP and MRCG-FCM as compated with control treatment. This result means that FPL has contained with favorable conditions for growing grass, because forage productivity is more than 15 tons per ha per year in fallow paddy land. In addition, the farmer can save the trouble of repeated plowing and sowing every year, due to the introduction of perennial grasses. The farmer must conduct the re-seeding and induce the improvement of management methods for the elevation of the persistence of red clover and perennial ryegrass, because both red clover and perennial ryegrass having high nutritive value and palatability was less persistent. Therefore, we suggest that FPL may be the good land for forage production utilizing FCM and FCM can be applied on FPL without any negative effects on DM production and the property of soil. FPL of Korea can be better utilized by applying FCM to the mono and/or mixed swards.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.4
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• pp.411-418
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• 2012

This study was conducted to develop the technique for cultivation of forage crops using swine slurry in fallow paddy land located in the mid-mountain area (FPL). The field experiments were carried out from 2007 to 2009 on FPL at Kumsan, Chungbuk province in Korea. Swine slurry was prepared which decayed for 6 months. The experimental plots were consisted of seven treatments; tall fescue-based mixed pasture applied with chemical fertilizer (Control), mono-tall fescue pasture (MTF), tall fescue-based mixed pasture (TFBM), mono-Perennial ryegrass (MPR), mono-Italian ryegrass (MIR), mono-Red clover (MRC and mono), Reed canarygrass (MRCG) applied with swine slurry. The field of tall fescue-based pasture had been sown with a grasses mixture containing 'Fawn' tall fescue, 'potomac' orchardgrass, 'Reveille' Perennial ryegrass, and 'kenblue' Kentuky bluegrass, 'Kenland' Red clover. Seeding rates were 16, 6, 4, 2 and 2 (kg) per ha, respectively. DM yields of forages and rates of grass coverage were higher in MTF, TFBM and MRCG as compared with control treatment. This result means that FPL has contained with favorable conditions for growing grass, because forage productivity is more than 14.5 tons per ha per year in fallow paddy land. In addition, the farmer can save the trouble of repeated plowing and sowing every year, with the introduction of perennial grasses. The farmer must conduct the re-seeding and induce the improvement of management methods for the elevation of the persistence of red clover and perennial ryegrass, because both red clover and perennial ryegrass having high nutritive value and palatability was less persistent. Therefore, we suggest that FPL may be the good land for forage production utilizing swine slurry and swine slurry can be applied on FPL without any negative effects on DM production and the property of soil. FPL of Korea can be better utilized by applying swine slurry to the mono and/or mixed swards.

• Journal of Bio-Environment Control
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• v.29 no.4
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• pp.337-343
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• 2020

This study was carried out to evaluate the yield of cuttings according to the planting and runner releasing times of mother plants in order to raise the cutting seedlings of raising seedling period 75 days or more needed for forcing culture of strawberries to be transplanted into the field around the 15^th of September. Three domestic cultivars of 'Maehyang', 'Jukhyang', and 'Kuemsil' were tested. For experiment 1 to determine the yield of cuttings with the change of transplanting time, the mother plant were planted on February 28, March 20, and April 9 in 20 days intervals, and the cuttings were collected two to three times from June 4 to July 1. Experiment 2 was conducted to investigate the yield of cuttings depending on the runner releasing time, the runners were released in three intervals of 20 days, 40 days, and 60 days after planting the mother plant on March 5, and the cutting were collected once to three times from May 29 to June 26. From the comparisons of cutting yield according to the transplanting time of mother plants, February 28 treatment was more 9~25% and 114~165% for each cultivar than March 20 and April 9, respectively (Experiment 1). The yield of cuttings with releasing time 20 days after planting the mother plants had higher by 60~77% and 104~176% for each cultivar than 40 days and 60 days, respectively (Experiment 2). From these results, in case of propagating the seedlings from cuttings needed for field planting around September 15, early planting around in the latter part of February is the best for cuttings yield. In addition, releasing after the removal of the runners produced from mother plants by 20 days after planting gives an advantage over higher yield of cuttings. Consequently, this study suggest to apply an efficient raising seedling system for labor saving and quality improvement in raising seedlings of three strawberry cultivars in Korea.