• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.5
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• pp.711-716
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• 2016

The purpose of this study was to evaluate the quality of castella added with different concentrations (0%, 5%, 10%, and 15%) of Panax ginseng sprout powder. The specific gravity of castella made with 15% ginseng sprout powder was the highest (0.65), and baking loss rate was highest well. However, the height and weight of castella were highest in the control. The pH of castella decreased with increasing levels of Panax ginseng sprout powder, whereas sugar content of castella did not show significant differences. The Hunter L, a, and b values of crust decreased as the concentration of Panax ginseng sprout powder increased. The Hunter L and a values of crumb decreased as the concentration of ginseng sprout powder increased, whereas b values of crumb increased. The gumminess and chewiness of castella increased by addition of Panax ginseng sprout powder, whereas cohesiveness of castella decreased. The hardness and springiness of castella did not show significant differences. In the sensory evaluation, crust color, crumb color, aroma and moistness did not show significant differences among samples while sweet taste, and chewiness were highest in the control group. The overall acceptability of castella added with 5% Panax ginseng sprout powder was the highest. Therefore, the results suggest that castella added with 5% ginseng sprout powder could be helpful for improving physical quality and taste.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.79-79
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• 2020

본 연구는 새싹인삼(Panax ginseng sprout) 재배용으로 이용하기 위한 묘삼(종삼)의 장기 저온저장 시 저장온도가 묘삼의 새싹인삼으로의 생장과 품질에 미치는 영향을 조사하여 묘삼의 저장에 적합한 온도를 구명하고자 수행하였다. 2년생 묘삼을 50 ㎛ LDPE 필름에 100g씩 넣어 포장하였고 온도 0, -2, -4℃에서 10개월 동안 저장하면서 2개월 마다 꺼내어 5℃ 저장고로 이동하여 5~7일 온도순화처리를 거친 후 실험을 실시하였다. 1차 육안조사로 묘삼의 부패율과 2차 생육조사로 묘삼을 재식 후 새싹인삼으로 재배 후 생장 상태와 품질을 조사한 결과 저장 4개월 이후 묘삼의 생육조사 시 새싹인삼의 생장과 품질이 묘삼의 저장온도에 따라 유의적인 차이를 보였다. 즉, 저장 4개월 된 묘삼의 건전한 새싹인삼으로의 생장은 0℃ 저장 묘삼에서 58.3%, -2℃ 저장 묘삼에서 72.1%, -4℃ 저장 묘삼에서 37.2%로 조사되었고, 저장 8개월 된 묘삼은 0℃ 저장 묘삼에서 9.7%, -2℃ 저장 묘삼에서 54.3%, -4℃ 저장 묘삼에서 6.9%로 조사되었다. 특히 0℃ 저장 묘삼은 저장 2개월에 출아가 진행되었고 저장기간이 지날수록 출아된 싹이 동해의 피해를 받는 것으로 조사되었다. 묘삼의 생육특성인 엽장, 엽폭, 경장, 근장을 측정한 결과 -2℃에 저장된 묘삼의 엽폭과 경장의 길이가 유의적으로 긴 것으로 조사되어 새싹인삼 재배를 위한 묘삼의 적정 저장온도는 -2℃로 설정하였다.

• Research in Plant Disease
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• v.28 no.4
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• pp.204-208
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• 2022

Hydrogen peroxide is an eco-friendly oxidizing agent, which has exhibited a broad spectrum of antimicrobial activity without adverse environmental impact. This study was conducted to investigate the antifungal effect of hydrogen peroxide treatment against Cylindrocarpon destructans, and consequently to evaluate its control efficacy against root rot disease of 2-year-old ginseng plants. Hydrogen peroxide treatment strongly inhibited the viability of C. destructans conidia in vitro. The hydrogen peroxide at a concentration of 300 mg/l significantly reduced disease infection of the ginseng root when treated to spore suspension (10⁷ conidia/ml). Spraying with 300 mg/l of hydrogen peroxide reduced the root rot disease of the ginseng sprouts by 15% compared to the untreated control at 14 days after the inoculation. However, 300 mg/l of hydrogen peroxide delayed the emergence of ginseng plants during sprouting under aeroponic conditions. Further works need to be done to provide an acceptable control efficacy of hydrogen peroxide against the disease and its good safety to ginseng plants.

• Journal of the Korea Convergence Society
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• v.13 no.1
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• pp.149-160
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• 2022

In this study, the physical quality and microbial changes of ginseng sprout according to the pretreatment process and packaging container were evaluated to improve the storage properties of ginseng sprout produced in smart farm. Quality change during storage (10 days) according to pretreatment method (ascorbic acid, citric acid, peroxyacetic acid) and packaging container (expanded polystyrene (EP), polypropylene (PP), polyethylene (PE), polypropylene + polyethylene + cast polypropylene (PP+PE+CPP)) was evaluated in terms of texture, viable cell count, water content, and color. As a result of comparison according to the type of pretreatment, the citric acid treatment group showed the lowest texture change and the effect on inhibition of bacterial growth. On the other hand, citric acid, which was most effective among pretreatments, was treated in all samples and then stored in 4 types of containers. Specially, the ginseng sprout in PP packaging container was not observed significant softening or color changes after 10 days storage, and the lowest changes in viable cell number. Therefore, this study was shown that citric acid treatment and use of PP packaging container are effective in increasing the shelf life of ginseng sprout.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.81-81
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• 2020

본 연구는 새싹인삼(Panax ginseng sprout) 재배용으로 이용하기 위한 묘삼(종삼)의 장기 저온저장 시 사용되는 필름의 종류가 묘삼의 출아율이나 생육에 영향을 미치는 지 조사하기 위해 수행되었다. 포장에 사용된 필름은 다공성물질인 제올라이트와 페그마타이트가 5% 함유된 50㎛ low density polyethylene(기능성 LDPE) 필름과 nylon/polyethylene 80 ㎛(Ny/PE) 필름에 묘삼을 100g씩 포장한 후 골판지 상자에 담아 -2℃의 저장고에서 10개월 동안 저장하면서 8개월째부터 2개월마다 시료를 꺼내어 5℃에서 5일 동안 온도 순화를 시킨 후 1차 조사에서는 육안조사로 실험실에서 건전한 묘삼과 부패 묘삼을 조사하였고, 건전한 묘삼만 새싹인삼 수경재배 농가에 재식한 후 2차 조사로 묘삼의 출아율과 출아된 묘삼이 새싹인삼으로 건전하게 생육한 비율을 조사하였다. 조사결과 Ny/PE 필름에 8개월 저장된 묘삼의 경우 1차 육안검사 시 뇌두부위가 검게 변하여 묘삼이 대부분 고사된 것으로 조사되었다. 필름 개봉 전 필름 내부의 O₂ 및 CO₂ 가스농도를 측정한 결과 O₂ 농도는 1.91%, CO₂ 농도는 38.9%로 측정되었고, 필름 개봉 시 알코올 냄새의 이취가 나는 것으로 보아 Ny/PE 필름으로 포장 된 묘삼이 -2℃의 저온저장기간 동안에도 호흡을 하면서 필름 내 산소를 완전히 소모하고 혐기적인 환경으로 변화시켜 대사활동이 불가능하였기 때문에 뇌두의 싹이 고사된 것으로 조사되었다. 반면 기능성 필름으로 포장한 묘삼은 저장 8개월에 정식 후 74.3%의 출아율을 보였고, 건전한 새싹인삼의 비율은 67.2%로 조사되었다. 정식 전·후 묘삼의 개체수 대비 건전 출아율의 비율은 56.7%를 나타내어 Ny/PE 필름으로 포장한 묘삼의 결과와 큰 차이를 보였다. 기능성필름의 개봉 전 필름 내부의 O₂ 및 CO₂ 가스농도를 측정한 결과 O₂ 농도는 12.9%, CO₂ 농도는 4.6%로 측정되어 묘삼의 저온저장 시 필름을 적용할 경우 공기의 유동이 원활이 이루어져 내부 O₂ 농도를 일정 수준 이상 유지해주는 필름을 사용하는 것이 묘삼의 출아와 생육에 좋은 것으로 조사되었다.

• Journal of Applied Biological Chemistry
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• v.63 no.4
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• pp.413-420
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• 2020

We studied the effects of the extract of aerial parts of hydroponically cultured ginseng (HGE) on alcohol-induced liver damage (AILD) in mice. AILD was induced by the oral administration of ethanol (EtOH) (25%; 5 g/kg body weight) for seven days in the study as well as EtOH-only groups. However, HGE (4 and 12 mg/kg) was orally administered (once daily for ten consecutive days) only to the study group, three days prior to the EtOH treatment. The HGE-treated group showed significantly lower levels of alanine aminotransferase and aspartate aminotransferase than the EtOH-only group. In addition, HGE administration decreased the level of serum lactate dehydrogenase, a known marker of liver damage. The effect of HGE on AILD was found to be dose dependent, and the consecutive administration of HGE showed no side effects in mice. Our study indicates that HGE treatment can potentially reduce oxidative stress and toxicity in the liver of alcohol-treated mice and that HGE can be a useful therapeutic agent for alcohol-induced hepatotoxicity. Additionally, a simple and efficient high-performance liquid chromatography-ultraviolet detection method was developed for determining the contents of four major ginsenosides in HGE. The aerial parts of hydroponically cultured ginseng were extracted using 70% fermented ethanol, and the contents of ginsenosides F5, F3, F1, and F2 in HGE were found to be 2.5, 4.4, 1.4, and 23.3 mg/g, respectively.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.118-118
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• 2018

인삼은 우리나라에서 오랜 역사동안 많은 연구가 진행되어 왔으며, 현재는 다양한 방법으로 홍삼과 흑삼으로 만들어 식품, 화장품, 의약품 등 다양한 방면으로 사용하고 있다. 본 연구에서 시중에서 구매한 새싹삼(인삼새싹) 잎/줄기에 함유된 진세노사이드(Re, Rg1, Rb1, Rg3, Rh1) 함량을 높이기 위하여 증숙과 초음파 추출조건에 관한 연구를 수행하여 우수한 항노화 소재를 개발하기 위하여 실시하였다. 실험은 새싹삼 잎/줄기를 증숙 온도와 시간의 조건에서 진세노사이드 함량이 가장 높은 조건을 선정하였으며, 선정된 조건의 새싹삼 잎/줄기에 파장과 출력에 대한 조건으로 초음파 추출을 진행하여 진세노사이드가 가장 높은 함량을 보이는 조건을 선정하였다. 그 결과 새싹삼 잎/줄기추출물(GSE; Ginseng Sprout Extract)의 진세노사이드 함량은 4.8 mg/g으로 확인되었으나 증숙공정을 통해 8.82 mg/g으로 함량이 증가되었으며, 상기 증숙된 새싹삼 잎/줄기에 초음파공정을 적용하여 추출한 새싹삼 잎/줄기초음파추출물(SU-GSE; Steaming & dry Ultrasonication-Ginseng Sprout Extract)에서는 최대 10.65 mg/g으로 함량이 증가되었다. 반면, 새싹삼 뿌리의 진세노사이드는 2.30 mg/g으로 확인되었으나 증숙공정을 통해 4.95 mg/g으로 함량이 증가되었으며, 초음파추출공정을 통해 최대 5.82 mg/g으로 함량이 증가된 것을 확인할 수 있었으나, 새싹삼 잎/줄기에 비해 진세노사이드 함량이 낮은 것을 확인하였다. 항노화 소재로의 활용가능성을 평가하기 위하여 새싹삼 잎/줄기추출물 GSE와 SU-GSE에 대한 세포생존률, 항산화 및 항노화에 대한 효능평가를 진행하였으며 GSE의 경우 $100{\mu}g/ml$에서 세포생존률이 82.4%를 보인 반면 SU-GSE에서는 $1,000{\mu}g/ml$의 농도에서 101.8%의 세포 생존률을 보였다. 항산화 활성의 경우 GSE와 SU-GSE $100{\mu}g/ml$ 농도에서 각각 52%와 81%의 항산화 활성을 나타냄으로써 SU-GES의 조건에서 항산화 활성이 우수한 것으로 확인되었다. 또한, 항노화 활성에 대한 실험결과 MMP-1 유전자 발현에 대한 억제율을 비교한 결과 GSE와 SU-GES $100{\mu}g/ml$의 농도에서 각각 18%와 29%의 억제율을 보임에 항노화 소재로의 활용가능성을 확인하였다.

• Journal of Applied Biological Chemistry
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• v.65 no.3
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• pp.129-142
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• 2022

Ginseng sprouts, which can be eaten from leaves to roots, has the advantage of not having to use pesticides without being affected by the season by using smart farms. The optimal cultivation timing of sprout ginseng was checked and the nutritional content and antioxidant activity were compared and analyzed. The values of total fatty acids and total minerals were no significant changes during the growth periods. The contents of total amino acids were slightly decreased to 45 days and after increased to 65 days. When the growth period was 65 days, arginine had the highest content of 3309.11 mg/100 g. The total phenolic contents were high at 3.73 GAE mg/g on the 45 days, and the total flavonoid contents were also the highest at 9.04 RE mg/g on the 45 days. The contents of total ginsenoside was not noticeable for the growth periods (29.83 on 25 days→32.77 on 45 days→26.02 mg/g on 65 days). The ginsenoside Rg2 (0.62 mg/g), Re (8.69 mg/g), Rb1 (4.75 mg/g) and Rd (3.47 mg/g) had highest contents on 45 days during growth. The values of phenolic acids and flavonols were gradually increased to 45 days (338.6 and 1277.14 ㎍/g) and then decreased to 65 days. The major compounds of phenolic acids and flavonols were confirmed to benzoic acid (99.03-142.33 ㎍/g) and epigallocatechin (416.03-554.64 ㎍/g), respectively. The values of 2,2-diphenyl-1-picrylhydrazyl (44.27%), 2,4,6-azino-bis (3-ethylbenzothiazoline-6-sulphnoic acid) diammonium salt (75.16%), and hydroxyl (63.29%) radical scavenging activities and ferric reducing/antioxidant power (1.573) showed the highest activity on the 45 days as well as results of total phenolic and total flavonoid contents.

• Korean Journal of Medicinal Crop Science
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• v.27 no.2
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• pp.126-135
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• 2019

Background: The ginseng ginsenosides, which have various physiological activities, are known to be more abundant in the leaves than in the roots, and the consumers' interest in ginseng sprout as a functional vegetable has been increasing. Methods and Results: The aim of this study was to investigate the effects of growth period on growth properties, active ingredients and rheology of ginseng sprouts cultivated in a non-heated greenhouse equipped with a shade net for 60 days, starting from the end of May to the middle of July. The chlorophyll content of the leaves decreased, but their length and width increased with increasing cultivation days. In particular, growth increased significantly until 40 days, but only slightly after 50 days. The stem length did not increase greatly from the 20 th to the 30 th day of cultivation, but increased significantly from the 30 th to the 40 th day, and then further increased gradually. The weight of the leaves, stems, and roots increased slightly, but not change significantly. After 40 days of cultivation, the total ginsenoside content increased by 1.07 times in the leaves and decreased by 0.80 times in the roots with increasing cultivation days. The leaf contents of ginsenosides $Rg_1$, Re, $Rb_1$, Rc, $F_3$ and $F_4$ increased with increasing cultivation days. The rheological properties of ginseng sprout showed the greatest influence on stem hardening with increasing cultivation days. Conclusions: Therefore, based on the growth characteristics, active ingredients and physical properties, 40 days after sowing was considered to be an appropriate harvesting time for ginseng sprouts.

• Journal of Bio-Environment Control
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• v.31 no.1
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• pp.28-34
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• 2022

This study was conducted to determine the changes in ginsenosides content according to additional UV-A, and UV-B LED irradiation before harvesting the ginseng sprouts. One-year-old ginseng seedlings (n=100) were transplanted in a tray containing a ginseng medium. The ginseng sprouts were grown for 37 days at a temperature of 20℃ (24h), a humidity of 70%, and an average light intensity of 80 µmol·m^-2·s^-1 (photoperiod; 24h) in a container-type plant factory. Ginseng sprouts were then transferred to a custom chamber equipped with UV-A (370 nm; 12.90 W·m^-2) and UV-B (300 nm; 0.31 W·m^-2) LEDs and treated for 3 days. Growth parameters and ginsenoside contents in shoot and root were conducted by harvesting on days 0 (control), 1, 2, and 3 of UV treatments, respectively. The growth parameters showed non-significant differences between the control and the UV treatments (wavelengths or the number of days). Ginsenoside contents of the shoot was highly improved by 186% in UV-A treatment compared to the control in 3 days of the treatment time. The ginsenoside contents of the roots was more improved in UV-A 1-day treatment and UV-B 3-day treatment, compared to the control by 171% and 160%, respectively. As a result of this experiment, it is thought that UV LED irradiation before harvesting can produce sprout ginseng with high ginsenoside contents in a plant factory.