• Journal of Bio-Environment Control
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• v.23 no.3
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• pp.235-243
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• 2014

This research was conducted to investigate the influence of leaching fractions (LF) in each irrigation or fertigation on plant growth and changes in chemical properties of root media during the production of seedling grafts of tomato. Two root media containing Sphagnum peat moss plus vermiculite (5:5, v/v, PV) and coir dust plus vermiculite (5:5, v/v, CV) were formulated and pre-planting fertilizers were incorporated during formulation. Then, each medium was packed into 50 cell (volume 33 cc) and 105 cell (volume 18 cc) trays and the rootstock (cv. J3B Strong) and scion (cv. Sunmyung) were grown, respectively. The seedlings were grafted at 31 days after sowing and then the cut seedling grafts (Sunmyung scion/J3B Strong rootstock) were planted into 50 cell plug trays containing each of the two root media. After induction of the graft union and new adventitious roots for 7 days, the seedling grafts were fed with fertilizer solution once a week containing 4 different N concentrations (0, 50, 100, $200mg{\cdot}L^{-1}$). When determined after 31 days from seed sowing, the highest fresh weights of the root stock seedlings were obtained with 0.75 LF in PV (8.96g/seedling) and CV (7.11g/seedling) mixes. The EC of the both mixes were 0.93 and $1.09dS{\cdot}m^{-1}$, respectively. The fresh weights of the scion seedlings 31 days after seed sowing were 4.29g with 0.50 LF in the PV and 3.13g with 0.50 LF in the CV. The root medium ECs of the two treatments were 0.76 and $1.34dS{\cdot}m^{-1}$, respectively. Fresh weights of the seedling grafts grown for 31 days were greatly influenced by post-planting fertilizer concentrations. The heavier plants were obtained in $100mg{\cdot}L^{-1}$ N treatment than any other treatments in same mixes. The substrate ECs in these two treatments were 0.98 and $1.93dS{\cdot}m^{-1}$, respectively, indicating that the desirable range of soluble salts in soil extracts is higher in the CV mix than the PV mix. Results of this study suggest that optimum EC range is different in each medium and LF need to be adjusted differently for each root medium to produce high quality seedling grafts of tomato.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.56-56
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• 2019

본 연구는 식용과 약용으로 이용되는 국내 자생식물인 야산고비(Onoclea sensibilis var. interrupta Maxim.)를 기내에서 대량증식하기 위한 조건을 구명하기 위하여 수행되었다. 무가온 온실에서 수집한 포자를 기내에서 발아시켜 전엽체를 획득하였으며, 계대배양하며 실험의 재료로 사용하였다. 전엽체의 대량증식을 위해 전엽체 0.3g을 메스로 균일하게 다진 후 증류수 1ml와 함께 배지에 고루 펼쳐서 배양하는 방법을 사용하였으며, 증식에 미치는 배지의 영향을 확인하기 위하여 1/4, 1/2, 1, 2배로 조절한 MS배지를 조성하여 8주간 배양하였다. 이후, 증식이 우수한 배지를 선정하여 sucrose와 질소급원의 농도를 조절하였으며, 활성탄을 첨가하여 증식에 미치는 영향을 확인하였다. 배지종류 실험의 결과, 생체중이 1MS에서 10.2g으로 초기 접종량에 비해 가장 많이 증가하였으며, 현미경을 이용하여 관찰한 결과에서도 정상적인 전엽체의 형태인 심장형으로 발달하였다. 증식이 우수하였던 1MS배지에 sucrose의 농도를 조절하여 배양한 결과에서는 1%의 처리구에서 가장 증식률이 좋았으며, 질소급원의 경우 30mM의 농도로 조절한 처리구가 가장 좋은 결과를 보였다. 배지 내 활성탄의 첨가는 처리구당 증가된 전엽체의 생체중이 유의적인 차이를 보이지 않았다. 포자체 대량 형성을 위한 적정 배양토의 혼합조건을 확인하기 위하여 원예상토, 피트모스, 펄라이트 및 마사토의 혼합비율을 5종류로 달리하여 조성하여 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하였다. 조성한 배양토에 기내에서 배양한 전엽체 1g을 증류수와 함께 블렌더를 이용하여 10초간 분쇄하여 토양표면에 고루 분주하였다. 이후 12주간 재배한 결과, 모든 토양조합에서 포자체가 형성되었다. 그중 원예상토와 마사토를 2:1(v:v)로 혼합한 토양에서 포트 당 405.0개로 가장 많은 포자체가 형성되었으며, 전체적인 생육 또한 비교적 양호한 결과를 보였다. 따라서 야산 고비의 전엽체 대량증식에 적합한 배지는 경제성과 생육수준을 고려하여 1%의 sucrose와 질소급원의 농도를 30mM로 조절한 1MS 배지가 적합하며, 포자체 대량생산을 위해서는 원예상토, 마사토를 2:1(v:v)로 혼합한 토양이 적합하다고 판단되었다.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.47-54
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• 2015

This research was conducted to investigate the changes in moisture retention capacities of expanded rice-hull (ERH)-based root media and their influences on the growth of mother and daughter plants in vegetative propagation of 'Seolhyang' strawberry. The proportion of water at the container capacity of ERH medium was in the range of 20 to 23%. This range was lower than the 60 to 66% of strawberry-specialized medium, the 30 to 34% of soil mother material (SMM) and the 30 to 35% of loamy sand. The moisture content of ERH was reduced to 10 to 12% at 8 hours after irrigation, and there were large variations among replications of ERH medium. Among four kinds of root media formulated to contain ERH, the medium of ERH + coir dust (CD) (55 + 45%, v/v) had 26.5 and 32.5% water contents at 20 and 40 days after irrigation to daughter plants, respectively. The m edia o f ERH + sandy loam (S L) and E RH + S MM showed similar trends i n moisture r etention. The pH and EC i n the all root media tested were in the range of 6.7 to 7.1 and 0.03 to $0.08dS{\cdot}m^{-1}$, respectively. The pHs and ECs measured at 20 and 40 days after irrigation were not significantly different in each root medium. Among the root media formulated to contain ERH, the growth of daughter plants was the highest in the treatment of ERH + SL (55 + 45%, v/v). As the blending rate of coir dust was elevated in the ERH + CD media, moisture retention capacity increased gradually, but the growth of daughter plants became worse even though the medium showed higher moisture retention capacity than other root media tested. The growth of roots and aboveground tissues of daughter plants deteriorated in the root media formulated by blending ERH + perlite (PE) at various ratios. The results of this research suggest the optimum formulations of root media and management of moisture content in raising of strawberry daughter plants when ERH is a component of root media.

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.658-666
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• 2020

The purpose of this study was to select a suitable planting substrate for multilayered plantings in an apartment landscape space. The experiment was conducted between May to October 2019, at the National Institute of Horticultural and Herbal Science. Planting substrate was prepared in six repetitions of eight treatment zones using mulching material, horticultural soil, bottom ash, and subgrade soil. Rosa hybrid 'Barkarole' and Hydrangea macrophylla 'Nikko Blue' were selected as the experimental plants. We investigated the monthly variation and effect of the substrate type on the growth (plant height, number of branches, leaf length, leaf width, and plant area of the substrates) of the plants. In R. hybrid 'Barkarole' grown in 20 cm of horticultural soil and 10 cm of bottom ash, the plants were taller(102.2±5.8 cm), had more branches (5.5±0.6 each), longer leaves (10.9±1.0 cm), and greater leaf width (6.2±0.5 cm) and plant area (4077.1±416.6 cm²)(p<0.05). H. macrophylla 'Nikko Blue' showed the best growth from 3cm of mulching, 20cm of horticultural topsoil, and 10cm of bottom ash, which resulted in taller plants (43.6±2.1 cm), more branches (4.9±0.8 each), longer leaves (7.2±0.5 cm), and greater leaf width(4.3±0.3 cm) and plant area (344.5±43.2 cm²). Through this study, it was possible to propose an optimal planting substrate for shrubs for multi-layered landscaping.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.22-22
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• 2018

실고사리[Lygodium japonicum (Thunb.) Sw.]는 국내 자생하는 덩굴성 양치식물로 유인재배가 용이하여 실내 외 조경 및 관상소재로 활용이 가능하다. 한방에서는 전초를 해금사초, 포자를 해금사, 뿌리와 지하경을 해금사근이라 약재로 사용한다. 본 연구는 관상 및 약재로 이용이 가능한 실고사리의 대량생산을 위한 기내 외 번식방법을 개발하고자 수행되었다. 식물재료는 경상북도 의성군 일대에서 성체를 수집하여 청주의 일반하우스에 식재하여 성숙한 포자를 채취하였다. 포자를 기내 발아시켜 전엽체를 획득한 다음 8주 간격으로 계대하면서 연구의 재료를 확보하였다. 전엽체의 증식과 생육에 적합한 배지를 비교 하고자, 1/4, 1/2, 1, 2MS배지와 Knop배지를 조성하여 배양하였다. 배양방법은 전엽체 300mg을 메스로 균일하게 다져서 배양하는 방법을 이용하였으며, 배양환경은 온도($25{\pm}1.0^{\circ}C$), 광도($30{\pm}1.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), 광주기(16/8h light/dark)로 조절되었다. 연구결과, 모든 처리구의 생체중은 초기접종량 보다 11배 이상 증가하였고, 그 중 1MS배지에서는 전엽체의 생체중이 7.3g으로 가장 많이 증가하였다. 뿐만 아니라, 형태형성도 우수하여 모두 정상적인 전엽체의 형태인 하트형으로 발달이 유도되었다. 전엽체로부터 포자체 형성을 유도하고자, 원예상토, 피트모스, 펄라이트 및 마사토의 혼합비율을 5종류로 달리하여 사각분($7.5{\times}7.5{\times}7.5cm$)에 혼합토양을 충진하였다. 전엽체 1g과 증류수 25mL를 핸드블랜더로 10초간 분쇄하여 사각분의 토양표면에 균일하게 분주하는 방법을 사용하였다. 이후 온도($25{\pm}1.0^{\circ}C$), 광도($43{\pm}2.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), 광주기(16/8h light/dark), 습도($72{\pm}2.0%$)를 유지하면서 10주간 재배되었다. 연구결과, 원예상토 단용, 원예상토와 펄라이트 및 마사토가 2:1(v:v)로 혼합된 토양에서 각 498.0, 402.5, 482.5개의 포자체가 형성되어 사각분 면적대비 7.16개($cm^2$)가 생산되었다. 한편 포자체의 생육은 원예상토와 펄라이트가 2:1(v:v)로 혼합된 토양에서 생체중, 엽장, 엽폭, 근장 및 SPAD value 등이 우수하였다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.57-57
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• 2019

본 연구는 관상 및 조경용으로 개발이 가능한 남방계 양치식물인 점고사리[Hypolepis punctata (Thunb.) Mett.]의 전엽체 증식 및 포자체 형성에 적합한 배양조건을 구명하고자 수행되었다. 무가온 온실에서 성숙한 포자엽을 채집한 후 포자를 기내에서 발아시켜 전엽체를 획득하였으며, 8주 간격으로 계대배양 하여 실험의 재료로 사용하였다. 배지종류에 따른 전엽체의 증식 및 형태형성을 확인하고자, 배양된 전엽체 0.3g을 메스로 잘게 다진 후, 농도를 1/4, 1/2, 1, 2배로 조절한 MS배지에 8주간 배양하였다. 이후 선발된 배지를 기준으로 sucrose, 활성탄, 질소급원의 농도를 조절하여 전엽체의 증식과 형태형성을 확인하였다. 그 결과, 1MS배지에서 전엽체의 생체중이 초기 접종량인 0.3g에 비해 10.7배 증가한 3.2g으로 가장 높은 증가율을 보였다. 형태관찰에서도 장정기의 형성이 관찰되었으며, 전엽체의 쿠션조직이 비교적 잘 발달하였다. 전엽체 증식에 가장 좋은 효과를 보인 1MS배지를 기준으로 sucrose의 농도를 0-4%로 달리하여 실험한 결과, 1%의 처리구에서 6.7g으로 가장 높은 생체중을 보였다. 활성탄의 농도를 0-0.8%로 첨가한 네 처리구 중에서는 0.8%의 처리구에서 14.2g으로 무처리구에 비해 생체중이 2배 이상 증가하였다. 전엽체의 형태 또한 정상적인 발달을 보였다. 질소급원의 비율을 30-120mM로 조절한 배지에서는 60mM의 처리구에서 4.9g으로 가장 높은 생체중을보였다. 이후 포자체 형성을 위한 최적의 토양조건을 구명하고자, 원예상토, 피트모스, 펄라이트 및 마사토의 비율을 달리하여 5종류의 배양토를 조성하였다. 혼합된 토양은 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진 하였으며, 배양된 전엽체 1g을 증류수와 함께 10초간 분쇄한 다음 준비된 토양표면에 분주하여 재배하였다. 12주간의 재배 결과, 원예상토를 단용한 토양에서 포자체의 수가 포트당 250.0개로 가장 많이 형성되었으며, 포자체의 생육 또한 다른 처리구에 비해 우수한 결과를 보였다. 한편 피트모스가 혼합된 토양에서는 포자체가 형성되지 않았다. 따라서 점고사리의 전엽체 대량증식에 적합한 배지는 sucrose 1%와 질소급원의 농도를 60mM, 활성탄을 0.8% 첨가한 1MS배지로 판단되며, 포자체 대량생산을 위해서는 원예상토를 단용한 토양이 적합하다고 판단된다.

• Journal of Korean Society of Forest Science
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• v.110 no.4
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• pp.577-589
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• 2021

Soil seed bank community contributes to the long-term conservation of plant diversity and vegetation dynamics, and their decreasing diversity and density with soil depth provide critical perspectives (deterministic and stochastic) for understanding the community disassembly process. We analyzed changes in species composition and diversity and structuring patterns by soil layer (top and bottom), including surface vegetation, in Gwangneung Forest, a mature forest with a vegetation climate in the temperate central part of the Korean Peninsula. From two layers of soil collected with a vertical difference of 10 cm, 934 specimens of 27 families, 40 genera, 44 species, three varieties, and 47 taxa, germinated. Although species diversity and germination density decreased in most comparative characteristics, including growth type, there was no statistical significance due to large deviations. Within-group variability of species composition was similar in the upper and lower soils, as was the decline pattern in co-occurred species (ζ-diversity) and change in species retention probability. The structuring process of the community composition in the two soil layers was fitted with an exponential correlation rather than a power function, demonstrating the dominance of the stochastic process. The pattern in diversity and species turnover according to soil depth in Gwangneung Forest was discovered to be structured by stochastic random events, such as seed vertical movement rather than interaction with trait characteristics.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.886-897
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• 2016

The physical properties of rooting media for the establishment of plugs in a greenhouse are modified according to variations in the greenhouse environment throughout the season. In this study, we established a standard for rooting media for the production of plug seedlings for each growing season (summer, winter and spring fall). Eight types of peatmoss (PM) and 4 types of perlite (PL) commonly used in Korea were collected and blended with the ratio of 7 parts PM to 3 parts PL (v/v) to make 32 different rooting media blends. We determined the total porosity (TP), container capacity (CC), air-filled porosity (AFP), pH, and electrical conductivity (EC) of the 32 media blends, and 6 media blends were selected for seasonal use. We also conducted additional analyses for plant easily available water (EAW), buffering water (BW), cation exchange capacity (CEC), and nutrient contents in the 6 media blends. The TP, CC, and AFP of the 32 media blends ranged from 64.7 to 96.0%, 42.9 to 90.1%, and 1.3 to 27.8%, respectively, indicating that the physical properties were strongly influenced by the type of PM and PL. The pH and EC of the PMs ranged from 2.96 to 3.81 and 0.08 to $0.47dS{\cdot}m^{-1}$, respectively. However, after blending the PM with the PL the pH was raised and the EC was lowered The media blends selected for the summer growing season were Blonde Golden peatmoss (BG) + No. 1 perlite size < 1 mm (PE1) and Latagro 0-10 mm (L1) + No. 2 perlite size 1-2 mm (PE2). These two media blends had 89.8-90.9% of TP, 80.8-81.3% of CC, and 9.0-9.7% of AFP. The media blends selected for the winter growing season were Sfagnumi Turvas (ST) + PE2 and Latagro 20-40 mm (L3) + PE2. These media blends had 79.9-86.7% of TP, 60.4-74.9% of CC, and 11.8-19.6% of AFP. The TP, CC, and AFP of two media blends, BG + No.3 perlite 2-5 mm (PE3) and Orange peatmoss (O) + PE3, selected for the spring and fall growing seasons, respectively, were 85.2-87.3%, 77.9%, and 7.4-9.4%, respectively. The percentage of EAW of the media blends selected for the spring, summer, and winter growing seasons ranged from 24.2-24.9%, 22.0-28.6%, and 18.0-21.8%, respectively, but the percentages of BW were not significantly different among the selected root media blends. The pH, EC, and CEC of the 6 selected media blends ranged from 3.11-3.97, $0.06-0.26dS{\cdot}m^{-1}$, and $97-119meq{\cdot}100g^{-1}$, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.514-520
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• 1996

To clarify the proper nitrogen application level for rasing rice infant seedling under different nursery soil, Dongjinbyeo was raised at seedling box with different basal nitrogen level. The results are as follows. The higher the nitrogen level was, the lower emergence rate and the lower establishment rate. The establishment rate was less than 90%, when the nitrogen was more than 2g/box in hill soil and more than 1g/box in paddy soil. The more the nitrogen level was, the higher the seedling height in hill soil, but was higher in the order of N-2, 3, 1 and 0g /box. Leaf number wasn't significantly different between nusery soils and among nitrogen levels when seedling was raised more than 6 days. The endosperm survival rate was decreased as the increased nitrogen level, but wasn't different between the nursery soils. Amount of root was decreased as the increased nitrogen level in paddy soil, but was heavier in the order of N-1, 0, 2 and 3g /box in hill soil. Mat formation was better as the nitrogen level was decreased in all nursery soils. Considering the emergence rate, seedling charactors and mat formation, the proper nitrogen levels seemed to be 2g /box for hill soil and 1g /box for paddy soil.

• Journal of agriculture & life science
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• v.50 no.2
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• pp.33-43
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• 2016

Pinellia tripartita(Blume) Schott is a herbaceous perennial plant belonging to the Araceae and distributed on Asia including of Korea, Japan, and China. P. tripartita is often used for gardening but has not been developed mass-breeding methods. In this study, we compared the tuber growth in different combinations of mixed soils used six compositions. Tubers used to study was cultured in vitro and divided into two groups. Type I was diameter more than 1cm and the group of Type II was diameter below than 1cm. Enlargement of tubers and growth of aerial parts were measuring the plant height, number of fresh leaves and dead leaves, number of bullets, tuber size, and fresh / dry weight. The size/weight and numbers of tubers from the mixed soil B (coir 68.0%, peat moss 14.7%, perlite 3.0%, vermiculite 7.0% and zeolite 7.0%) were the best grown up for eight weeks. In case of Type I, GI (Growth index) of tuber size and weight were 45% and 101%, respectively. The difference of growth was doubled compared to the bad growth treatment as the mixed soil E(Coir 14.3%, peat moss 14.3%, perlite 42.9%, vermiculite 14.3%, and zeolite 14.3%). These results could be used as the basic information for the similar experimental design for the P. ternata.