• Title/Summary/Keyword: 상선

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Regulation of hPTH Expression In Virto Using the Tetracycline Inducible Retrovirus Vector System (Tetracycline Inducible Retrovirus Vector System을 이용한 In Vitro에서의 인간 부갑상선 호르몬의 발현 조절)

  • Koo, Bon-Chul;Kwon, Mo-Sun;Kim, Te-Oan
    • Reproductive and Developmental Biology
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    • v.30 no.3
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    • pp.157-162
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    • 2006
  • Endogenous 84 amino acid parathyroid hormone(PTH) is synthesized as a pre-pro hormone by the chief cells of the parathyroid glands. Physiological actions of PTH include regulation of bone metabolism, renal tubular reabsorption of calcium and phosphate, and intestinal calcium absorption. In addition, PTH stimulates new bone formation by extraordinary stimulation of osteoblastic activity and decreasing calcium excretion by the kidney. In this study, we constructed and tested retrovirus vectors designed to express the human parathyroid hormone(hPTH) gene under the control of the tetracycline-inducible promoters. To increase the hPTH gene expression at turn-on state, woodchuck hepatitis virus posttranscriptional regulatory element(WPRE) sequence was also introduced into retrovirus vector at downstream region of either the hPTH gene or the sequence encoding reverse tetracycline-controlled transactivator(rtTA). Transformed primary culture cells(porcine fetal fibroblast, PFF, chicken embryonic fibroblast, CEF) were cultured in the medium supplemented with or without doxycycline(tetracycline derivative) for 48 hours, and induction efficiency was measured by comparing the hPTH gene expression level using two step RT-PCR and ELISA Higher hPTH expression($3{\tims}10^4\;pg/ml,\;5.3{\times}10^4\;pg/ml$) and tighter expression control(up to 8 fold) were observed from the vector in which the WPRE sequence was placed at downstream of the hPTH gene. The resulting tetracycline inducible vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals.

A Questionnaire Survey on Disease and Medical Management of Seafares' (상선승무원들의 상병 및 의료관리 실태 조사)

  • Kim Jae-Ho;Moon Sung-Bae;Ha Hae-Dong;Yang Won-Jae;Lee Sang-Woo
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2006.06b
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    • pp.19-27
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    • 2006
  • The purpose of this study was to find of morbidity rate and pattern of disease and affect of variables related disease and medical management of seafares' The subjects this study were 1049 seafares' who took education in Korea Institute of Maritime and Fisheries Technology. This questionnaire was focused on finding the basic data for prevention of disease and promotion health for the seafares' The collected data were analyzed by using descriptive statistics, Chi-square, cross tab, linear regression by SPSS 10.1 package. The result of this study are as follow. 1)The morbidity rate within recent 12 months was 69.0%. 2)there were significant differences of occurred disease in age(p<0.05), income(p<0.01), career of ship on board(p<0.01), rank(p<0.01), perceived health status(p<0.01), worry of health(p<0.01), fatigue symptoms(0.01), satisfy of job(p<0.05), rest time(p<0.05) 3) Considering disease unable to work more than 4 hour, the number of those who had oral disease 7.3%> tinea 6.6%> gastric ulcer 6.4, and musculoskeletal disease group were 20.9%, which revealed the highest rate oral disease 13.6%> skin disease> 12.4%, digestive disease> 12.1%. 4) As refer to medical management, The pain above 31days 35.7%, hospitalization and treatment below 7days were each 50.2%, 42.8%, medical service were doctor's office 27.9, which revealed the highest rate.

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읽을거리 - 항공기 실전원리(12) - 바다 속 기뢰를 찾아라, 소해헬기(AMCM)

  • 한국항공우주산업진흥협회
    • Aerospace Industry
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    • s.107
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    • pp.68-69
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    • 2010
  • 기뢰(機雷, naval mine) 또는 수뢰((水雷)는 수중에 설치되어 함선이 접근 또는 접촉했을 때, 자동 또는 원격 조작에 의해 폭발하는 수중 병기다. 저렴하고 단순하지만 가장 현대적이고 강력한 해군조차도 겁내하는 해중무기다. 적상선 및 잠수함 공격에 효과적인 무기로 미국 남북전쟁을 기점으로 대량 사용되었다. 기뢰의 다양화, 지능화에 따라 적의 기뢰를 찾아 파괴하는 소해임무도 함께 강조되고 있다. 함정의 안전한 해상작전을 위해서는 반드시 따라야 하는 대기뢰전, 특히 헬기를 이용한 '공중 대기뢰전(Airborne Mine countermeasures)'은 현대 해군의 필수다. 세계 주요 소해헬기와 관련 기술에 대해 소개한다.

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Optimal Timing of Technology Transition from New Perspectives (기술 교체의 최적 시점에 관한 고찰)

  • Park, Sang-Seon;Kim, Bo-Won
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 2005.05a
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    • pp.1063-1067
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    • 2005
  • 기술개발 경쟁이 점점 치열해 지면서 기업의 입장에서 언제 신기술을 도입하여 사용할지에 대한 고민도 늘어가고 있다. 우리는 본 연구에서 기존 기술과 신기술이 공존 할 때 어떤 시점에서 새로운 기술로 기존 기술을 교체하는 것이 최적인가에 대해 고찰하고자 하였다. 이 문제에 대한 기존 연구에 대한 분석을 토대로 개념적인 수리적 모델을 제시하고 있으며 향후 이 연구가 어떠한 방향으로 이루어져야 하는지에 대한 방향을 모색하고자 한다.

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A Study on the Interior Design of Merchant Ship (상선설계의 실내디자인에 관한 연구)

  • 박명규;신영균;손철호
    • Journal of the Korean Institute of Navigation
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    • v.22 no.3
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    • pp.35-42
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    • 1998
  • When design the crew's living quarter, it is very important to consider the comfort for crew as well as its function. In this paper, design concepts of crew's living quarter are investigated based upon the questionnaire survey and the respondents who have been experienced going on board . Also, some improvements for interior design of the merchant ship are presented.

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LNG 선박 건조현황과 인력수요 예측

  • Park, Jin-Su
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • v.2
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    • pp.93-95
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    • 2006
  • 최근 LNG 선박의 발주량이 급증함에 따라 LNG 선에 승무할 인력의 심각한 부족이 예상되고 있다. 이러한 LNG 선박에 승무할 인력 특히 고급 사관의 부족은 선박 자체의 안전뿐 아니라 궁극적으로는 LNG 수송체계의 안전에도 큰 영향을 미치게 될 것이다. 이 발표에서는 현재 전세계 상선대의 현황에서 출발하여, 최근 LNG 선박의 발주 현황, LNG 선박에 적용되는 최신 기술 및 엔진 등에 대하여 살펴 본 후에 전세계 LNG 선박 수주 잔량을 근거로 하여 필요 인력을 예측해 본다.

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A Case of a Kitten with Nutritional Secondary Hyperparathyroidism (새끼 고양이에서의 영양학적 원인에 의한 부갑상선 기능항진증 증례)

  • 박우대;김휘율;배춘식;김희정;윤화중;장경진
    • Journal of Veterinary Clinics
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    • v.14 no.1
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    • pp.131-135
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    • 1997
  • Nutritional secondary hyperparathyroidism in a seven-month old male kitten showing signs of paraparesis (ataxia, dragging the pelvic limbs), abdominal distention, aconuresis was diagnosed with clinical signs, radiographs and serum biochemical tests. In radiographs, bones were abnormally radiolucent and cortices were thin. Serum biochemical tests were performed, but had normal values. The treatment was directed at the suspected dietary calcium and phosphorus imbalance. Oral calcium supplement and a commercial cat food were introduced. On clinical evaluation 3 months later, this case showed no clinical signs.

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`90 대한민국학생발명전 성대히 열려 - 6.26~7.15까지, 7.5 시상식

  • 한국발명진흥회
    • 발명특허
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    • v.15 no.7 s.173
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    • pp.5-17
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    • 1990
  • `90대한민국학생발명전시회가 지난 6월 26일 부터 7월 15일까지 20일동안 본회 발명장려관에서 성대하게 열렸다. 6월 26일 오전 11시 박필수 상공부장관, 이재근 국회상공위원장, 김철수 특허청장, 김상준 서울시 교육감, 김생기 본회회장, 박우영 조선일보사 사장, 이헌조 금성사 사장, 남상선 대한변리사회 회장 등이 개막 테이프를 끊음으로써 막이 오른 이번 전시회는 7월5일 오후 2시 시상식을 전후로 절정을 이루었다. 이번 전시회는 전국에서 5만 여명의 학생과 일반인이 관람한 것으로 밝혀졌다.

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A Design and Implementation of Fuzzy Controller for Temperature Control (온도 제어를 위한 퍼지 제어기의 설계 및 구현)

  • Seo, Kang-Myun;Park, Sang-Sun;Kang, Moon-Sung
    • Proceedings of the KIEE Conference
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    • 1997.07b
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    • pp.732-734
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    • 1997
  • This paper describes the design and implementation of a fuzzy temperature control system to control the water temperature in plant. For evaluating the performance of a fuzzy controller, the experiment results of a fuzzy controller are compared with these of a conventional PID controller which provides an auto-tuning function. The experiment results show that the proposed controller has a good control performance and is robust to external disturbance.

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