• Korean Journal of Environment and Ecology
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• v.25 no.5
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• pp.685-690
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• 2011

To search for myrmecochorous plant species in Korea and their diaspore characteristics, the flowering and fruiting of herb species, collectiong their fruits and diaspores, and the related ant species were studied from April to September 2011. Twenty-four herb species growing in Korea prove myrmecochorous (seed attached elaiosome) plants. Elaiosomes of 24 herb species vary considerably in shape and size. Major ant species transporting the diaspore of myrmecochorous plants are Formica japonica, Lasius alienus, Tetramorium tsushimae, Temnothorax koreanus, Lasius japonicus, but Camponotus japonicus and Paratrechina sakurae do not transport the diaspores. This study found out that mutualism between myrmecochorous plants and ants is carried out in Korea. Further study on the myrmecochorous plant and related ant species, cafeteria experiments should be needed.

• Korean Journal of Environment and Ecology
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• v.24 no.3
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• pp.258-263
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• 2010

To study on the typical domatia structure, the leaves of six broad-leaved tree species were examined. Domatia of the leaves of the broad-leaved trees is the defense strategies against hervivory of many broad-leaved tree species. We observed four type domatia; Pouch type, Tuft type, Pocket type, and Pocket + tuft type. This result suggests that broad-leaved trees have develope leaf domatia structures for mutualism with predatory mites.

• Korean Journal of Environment and Ecology
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• v.28 no.4
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• pp.419-423
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• 2014

To search for native myrmecochorous plant species in Korea and their diaspore characteristics, collecting their fruits and diaspores, and the related ant species were studied from April 2012 to October 2013. Eight herb species growing in Korea are added to the myrmecochorous (seed attached elaiosome) plant list. Elaiosomes of eight herb species vary considerably in shape and size. Major ant species transporting the diaspore of myrmecochorous plants are Formica japonica, Lasius alienus, L. japonicus, Myrmica lobicornis, Temnothorax spinosior, Tetramorium tsushimae, but Camponotus japonicus and Nylanderia sakurae do not transport the diaspores. This study found out that mutualism between myrmecochorous summer-flowering plants and ants is carried out in Korea. Further research on the native myrmecochorous plants should be needed.

• Korean Journal of Environment and Ecology
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• v.24 no.5
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• pp.611-616
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• 2010

To explore on the defense strategies against hervivory of broadleaved tree species growing in tropical rain forest, Solomon Islands, morphological characteristics of the leaf, leaf domatia structure and the number, herbivores insects and mites on the leaves of 26 tree species, collected from the trees growing in Guadalcanal, New Georgia, and Tetepare islands, were investigated from Feb. 25 to March 5, 2010. The results are summarized as follows. Six tree species including Terminalia catappa(23.08%) among total 26 species have domatia structure. Especially, domatia of T. catappa reveals two types; pouch type and pit+tuft type. Predatory mites are observed on the leaves of only three species including T. catappa. Predatory mites' number per leaf or leaflet proved a few, 0.33~0.40. Insect pests destroying the leaf of tree species are such as aphids, Diaspididae sp., larva of Lepidoptera, and Eriosoma sp.(Pemphlogidae). Ants are co-works with aphids and Diaspididae sp., and supports herbivores. These results indicate that protective mutualisms between tree and predatory mites may be less frequent in woody species of tropical rain forest in Solomon Islands.

• Korean Journal of Environment and Ecology
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• v.23 no.5
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• pp.411-417
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• 2009

This research is intended to discover physiological and biochemical defense mechanisms against herbivores of 8 species of the genus Acerby examining the morphological characteristics of the leaf, the structure and number of leaf domatia, herbivores insects and mites feeding on leaves, which were collected from the trees growing in Mt. Chiak, Mt. Cheongtae, Mt. Jungwang, Mt. Bangtae, Mt. Taebaek and Wonju City. This research was conducted from May through July, 2009, and the results are as follows. The domatia of A. pseudosieboldianum, A. pictum subsp. mono and A. mandshuricum belongs to tuft type; that of A. palmatum, A. triflorum and A. tschonoskii pocket+tuft type; and that of A. tegmentosum pocket type. The number of domatia per leaf turned out to be the highest in the case of A. tegmentosum(20.2), and the lowest in the case of A. ginnala (4.2). Leaf surface trichomes of A. palmatum, A. pseudosiebotdianum, A. ginnala and A. mandshuricum are covered with villi; those of A. pictum subsp. mono and A. triflorum with soft, pilose type of hair, and those of A. tschonoskii with stiff, strigose hair. The trichome density of lower leaf surface is found to be higher than that of the upper leaf surface. Only in the case of A. palmatum, A. pseudosieboldianum, A. tegmentosum and A. tschonoskii, a small amount of nectar is found to be secreted from the distal vein parts of the leaf margin. The number of mites on each leaf are found to be significantly different among tree species, and average mites number per leaf was the highest in the case of A. tschonoskii (9.2/leaf), and A. mandshuricum, A. pseudosieboldianum, and A. triflorum follow it in decreasing order. Minute insects attacking the leaf of Acer spp. include Periphyllus californiensis, P. viridis, Psylla spp, and gall mites, and the number of these insects are found in Periphyllus californiensis, Psylla spp, P. viridis, and Cicadellidae sp. in decreasing order. The natural enemies of these herbivores insects are predatory mites, such as Chilocorus rubidus, Coccinella septempunctata and the nymph, Aphidius ervi, Poecilocoris lewisi and its larva, and Poecilocoris lewisi. Lasius japonicus and Formica japonica are symbiotic with aphids, and supports herbivores. Finally, our research confirmed that predatory mites attack Periphyllus californiensis and suck the body fluid of their victim. This proves that mites form a symbiotic relationship with plants through the mediation of leaf domatia. This also indicates that a protective mutualism may be more conspicuous in temperate broad-leaved trees.

• Korean Journal of Environment and Ecology
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• v.26 no.3
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• pp.362-366
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• 2012

To explore on the defense strategies against hervivory of five Viburnum species, morphological characteristics of the leaf, leaf domatia structure and the number, herbivores insects and mites on the leaves, collected from the trees growing in Wonju-si, Pyeungchang-gun, Taebaek-si, Taean-gun, Bonghwa-gun, Sancheung-gun and Jinju-si were investigated from May 2009 to October 2011. Domatia of V. carlesii reveals pocket type, these of V. burejaeticum, V. dilatatum and V. wrightii reveal tuft type, and that of V. odoratissimum var. awabuki reveals pouch type. Domatia number per leaf proves the highest figures, 24.0/leaf for V. burejaeticum, and the lowest, 4.9/leaf for V. carlesii. Leaf surface trichomes of four Viburnum species except for V. odoratissimum var. awabuki are mainly stellate and hispid on the leaf-blade and veins, and dense stellate trichomes on the upper leaf surface of V. carlesii, V. burejaeticum, and V. dilatatum are observed till late growing season. Extrafloral nectaries(EFN) on the terminal veins of five Viburnum species are observed. Pellucid dots on the lower leaf surface of V. dilatatum and V. wrightii might be a good defense strategies against herbivores. We observe many predatory mites eating nectar on large pellucid dots of V. dilatatum. Predatory mites number per leaf proved highly significant differences among tree species, and mean of mites number was highest values 8.1/leaf for V. burejaeticum, and the lowest 2.6/leaf for V. odoratissimum var. awabuki. These results indicate that temperate broadleave trees develop various defense strategies against herbivores for survival. The more studies on the defense strategies against herbivores of tree species might be needed for sustainable forest ecosystem.

• Korean Journal of Environment and Ecology
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• v.26 no.2
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• pp.200-209
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• 2012

To search for the co-relationships between insect-pollinators and the plant species of Asteraceae, insects visiting in flower and the flowers in Korea, were studied from April 2010 to October 2011. The sum of flower visiting degrees are shown 38 in Lepidoptera, 38 in Diptera, 36 in Hymenoptera, and the lowest 6 in Coleoptera, respectively. 65 insect species are identified pollinators, Hymenoptera 13 species(Apidae 11 sp., Formicidae 2 sp.), Lepidoptera 29 species(Pieridae 5sp., Nymphalidae 12 sp., Satyridae 3 sp., Hesperirdae 3 sp., Lycaenidae 2 sp., Danaidae 1 sp., Moth 2 sp.), Diptera 16 species(Tachinidae 1 sp., Syrphidae 12 sp., Muscidae 1sp., Others 2 sp.), and Coleoptera 6 species(Cetoniidae 1 sp., Cermbycidae 3 sp., Chrysomelidae 1 sp., Mordellidae 1 sp.). 31 pollinator species visits the flower of $Erigeron$ $annuus$, next 15 pollinator species does the flower of $Eupatorium$ $japonicum$, and then 13 pollinator species does the flower of $Aster$ $ageratoides$. Only 2 pollinator species visit the flower of $Tephroseris$ $kirilowii$, $Ixeridium$ $dentatum$, $Inula$ $britannica$ var. $japonica$, $Carduus$ $crispus$, $Ligularia$ $fischeri$, $Ainsliaea$ $acerifolia$, $Synurus$ $deltoides$, $Cirsium$ $setidens$, $Crepidiastrum$ $enticulatum$ and $Dendranthema$ $boreale$. Pollinators of Lepidoptera visit more frequently white flower than yellow or purple one. This study found out that mutualisic relations between plants and insect pollinators is carried out in Korea.

• Korean Journal of Environment and Ecology
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• v.27 no.1
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• pp.22-29
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• 2013

This research was carried out for revealing the co-relationships between insect-pollinators and the plant species of Labiatae and Umbelliferae, insects visiting in flower and the flowers in Korea, were studied from April 2010 to August 2012. The sum of flower visiting degrees in Labiatae are shown 21 in Hymenoptera, 16 in Lepidoptera, and the lowest 1 in Diptera and Coleoptera, respectively. The sum of flower visiting degrees in Umbelliferae are shown 27 in Diptera, 24 in Hymenoptera, 21 in Coleoptera, and 13 in Lepidoptera, respectively. 17 pollinator species visited the flower of Angelica gigas, next 15 pollinator species did the flower of Agastache rugosa, 13 pollinator species did the flower of Prunella vulgaris var. lilacina. 13 pollinator species foraged on the flower of Peucedanum terebinthaceum. and then 11 pollinator species on the flower of Angelica dahurica. Only 1 pollinator species visited the flower of Clinopodium chinense var. grandiflora and Isodon excisus. Pollinators of Coleoptera and Diptera visit more frequently to the flowers of Umbelliferae than those of Labiatae. Pollinators of Lepidoptera and Diptera visit more frequently white flower than purple one. This study found out that mutualistic relations between plants and insect pollinators in Korea.

• Korean Journal of Environment and Ecology
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• v.26 no.1
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• pp.39-45
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• 2012

To explore on the defense strategies against hervivory of 67 broadleaved tree species, morphological characteristics of the leaf, leaf domatia structure and the number, herbivores insects and mites on the leaves, collected from the trees growing in Taean-gun, Chuncheongnam-do were investigated. 46 broadleaved tree species(68.7%) had the domatia structures, and 21 species including Quercus salicina and Magnolia grandiflora did not. 31 species including Juglans mandshurica and Carpinus laxiflora reveals tuft type, 12 species including Quercus dentata and Corylus heterophylla reveals pocket+tuft type, and 2 species, Sorbus alnifolia and Prunus yedoensis does pocket type, and Viburnum odoratissimum var. awabuki does pouch type. Domatia number per leaf proves the highest figures, 23.4/leaf for Quercus dentata. Plant defense strategies using leaf lower-surface trichomes of Magnolia grandiflora reveals dense villous, those of Populus alba and Vitis vinifera reveals dense pilose, that of Elaeagnus umbellata does dense scaly hairs, that of Pueraria lobata does dense strigose. Plant defense strategies using extrafloral nectaries were adapted 23 tree species(34.3%). Observed examples are Prunus tomentosa, Ficus carica, Viburnum dilatatum and Carpinus laxiflora. Predatory mites were observed on the leaves of 40 tree species(59.7%), and mean values of predatory mites was highest values 23.4/leaf in Quercus dentata. Minute arthropods destroying the leaf of broadleaved trees. are such as Periphyllus californiensis, P. viridis, Diaspididae sp., gall mites, thrips, and total numbers observed were odered gall mites, Diaspididae sp., aphids and thrips. Natural enemies of these hervivores arthropods are such as predatory mites, Chilocorus rubidus, Coccinella septempunctata and the nymph, Aphidius ervi. These results indicate that defense strategies including protective mutualisms may be frequent in the temperate broadleave trees.

• Journal of Applied Biological Chemistry
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• v.52 no.3
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• pp.116-120
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• 2009

We found out the new method of the consortium for the environmental friendly agriculture by 8 kinds of the selected antagonistic rhizobacteria. This research involved composition of mutual complementary consortium by each antagonistic function such as production of antibiotic, siderophore, antifungal cellulase and insoluble phosphate solubilization. The consortium No.11 among composed consortium candidates showed the most pepper growth promoting activity and Phytophthora blight suppression on the in vivo pot test of red-pepper plant. The consortium No. 11 is combination of PGPR Bacillus subtilis AH18 and Bacillus licheniformis K11. B. subtilis AH18 and B. licheniformis K11 both could produce the auxin, antifungal ${\beta}$-glucannase and siderophore. Also, they had mechanism for solubilization of insoluble phosphate. But, B. licheniformis K11 could produce the antibiotic of iturin which was able to inhibit Phytophthora capsici. We confirmed complementary noncompetitive mutualism between B. subtilis AH18 and B. licheniformis K11 of the consortium No.11. The results came out through treatment of two strains co-culture, treatment of individual culture and co-treatment of two individual cultures for the growth and Phytophthora blight suppression of red-pepper. The treatment of two strains co-culture didn't show a synergic effect in comparing sole treatment on the pepper growth promotion and Phytophthora blight suppression. But, when the pots were treated simultaneously with co-treatment of two individual cultures, an synergic effect was seen in the growth promotion of roots, stem, leaves and suppressed Phytophthora blight on red-pepper in vivo pot test.