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Regulatory Characterization of xylA Promoter Region in Escherichia coli (대장균의 xylA 프로모터 영역의 조절 특성)

  • Kang, Byung-Tae;Roh, Dong-Hyun;Joo, Gil-Jae;Rhee, In-Koo
    • Applied Biological Chemistry
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    • v.39 no.6
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    • pp.443-448
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    • 1996
  • In order to investigate the function of xylA promoter(Pxyl) as regulatory region Pxyl-lacZ fusion gene was constructed by the insertion of xylA promoter to the multiple cloning site of upstream of lacZ gene in a multicopy numbered plasmid pMC1403 containing promoterless lac operon, which was designated pMCX191, and Pxyl-lacZ fragment from pMCX191 was inserted to low copy numbered plasmid pLG339, designated pLGX191. The expressions of ${\beta}-galactosidase$ in these recombinant plasmids containing Pxyl-lacZ fusion gene were induced strongly by the addition of xylose, repressed by the addition of 0.2% glucose in the presence of xylose. The catabolite repressions were derepressed by the addition of 1 mM cAMP as same as native xylA gene. The fragment of xylA promoter was partially deleted from the upstream of xylA promoter by exonuclease III to investigate the regulation site of xylA promoter and the degrees of deletion derivatives of xylA promoter were analyzed by the DNA base sequencing. By the investigations of the induction by xylose, repression by glucose and derepression by cAMP on xylose isomerase production, the regulation site of xylA promoter may be located in segment between -165 and -59 bp upstream from the initiation site of xylA translation.

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Effect of Wangsuk Stream on NOM and Chlorinated DBPFPs in Han River Water (왕숙천 유입에 따른 한강본류의 천연유기물질과 염소소독부산물 생성능 변화)

  • Park, Hyeon;Kim, Chang-Mo;Chang, Hyun-Seong;Kim, Hyun-Suk;Park, Chang-Min;Yu, Myong-Jin
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.10
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    • pp.1031-1037
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    • 2006
  • The main purposes of this study were to compare the characteristics of fractionated natural organic matters(NOM) from Han River water and Wangsuk(W) stream water, and to investigate the relationships between NOM and the formation of disinfection by products(DBPs). Three types of resin such as XAD-4, XAD-7HP and IRC-50 were used to isolate the water samples into three organic fractions. The DOC concentrations of raw waters were relatively low($1.5{\sim}3.3$ mg/L) at all seasons. The hydrophilic was the major constituent, contributing $44{\sim}63%$ of the total NOM and hydrophobic $21{\sim}33%$, transphilic $16{\sim}31%$, respectively. The formation of trihalomethans(THMs) was highly influenced by particulated NOM especially in the rainy season, whereas haloaceticacid forming potentials(HAAFPs) depended more on the hydrophilic fraction of dissolved NOM which is known to be difficult to be removed through conventional processes. The NOM of W stream was characterized as 15% hydrophobic, 9% transphilic, and 76% hydrophilic. In the fractionation of NOM using resins, $20{\sim}40%$ of the NOM in W tributary water could not be clearly isolated, whereas, 85% of the NOM in the raw water was recovered. Although the DOC concentration of tributary water was higher than the raw waters from the Han River, the DBPFPs was approximately 40% of the raw waters. In DBPFPs aspect, W stream has less effect than Han River water itself. Bromide in tributary waters discharged from waste water treatment plants has been found to shift the distribution of THMs and HANs to the more brominated DBPs.

Distribution Patterns of Wintering Waterbird Communities in Urban Streams in Seoul, Korea (서울 도시하천에서 월동하는 수조류의 분포 특성)

  • Kwon, Young-Soo;Nam, Hyung-Kyu;Yoo, Jeong-Chil;Park, Young-Seuk
    • Korean Journal of Environment and Ecology
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    • v.21 no.1
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    • pp.55-66
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    • 2007
  • This study was conducted to analyze the distribution patterns of wintering waterbird communities in relation to local environmental factors in the urban streams of Seoul, Korea. A field survey was conducted at 66 sites of 5 tributaries and the main channel of the Hangang River in Seoul in January 2006. The total of 65 species and 39,560 individuals were recorded in the field survey. There were 48 species and 28,989 individuals in the Hangang River, 14 species and 1,395 individuals in the Tancheon stream, 15 species and 2,306 individuals in the Jungrangcheon stream, 22 species and 5,990 individuals in the Anyangcheon, 18 species and 283 individuals in the Changrungcheon stream, and 24 species and 597 individuals in Gokrungcheon stream. The dominant species were Anas platyrhynchos (22.65%), A. poecilorhyncha (14.01%), Aythya ferina (13.26%), Aythya fuligula (8.04%), and Mergus merganser (7.03%). Among the 16 species (with 30,650 individuals) of ducks, the dabbling and diving ducks were 8 species with 18,286 individuals and 8 species with 12,364 individuals, respectively. Through the principal component analysis, the study sites were classified into four main groups according to the similarity of their waterbirds' species compositions: 3 tributaries in the urban area (Group 1), 2 tributaries in the rural area (Group 2), one in the rural area, one in the urban area, the urban area in Hangang River (Group 3) and the main channel of the Hangang River in the urban area (Group 4). Species diversity index and species evenness were the highest in Group 1, while the lowest Group 2. Analysis on their environmental factors showed that the waterbirds wintering in urban streams of Seoul prefer broad water width, low water depth and broad resting sites.

Health Condition Assessment Using the Riparian Vegetation Index and Vegetation Analysis of Geumgang mainstream and Mihocheon (수변식생지수를 이용한 금강본류와 미호천의 건강성 평가 및 식생분석)

  • Lee, Seung-Yeon;Jang, Rae-Ha;Han, Young-Sub;Jung, Young-Ho;Lee, Soo-In;Lee, Eung-Pill;You, Young-Han
    • Korean Journal of Environment and Ecology
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    • v.32 no.1
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    • pp.105-117
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    • 2018
  • This study conducted health assessment and multivariate vegetation analysis using the riparian vegetation index in 30 sites of the Geumgang mainstream and Mihocheon to obtain practical data on the river management of the Geumgang. The result showed that the number of plant communities was 54. The flora was 75 families, 185 genera, 243 species, 2 subspecies, 21 varieties, 2 varieties, and 268 taxa. The riparian vegetation index was 38.3 (3.3; G-D1 ~ 66.7; G-U2, G-U4, and G-M3), and the health of the rivers in this area was evaluated as normal (grade C). The health of rivers was the highest in the upper stream of Geumgang mainstream and lowest in the downstream of Geumgang mainstream. The relationship between riparian vegetation index and chlorophyll-a content was low. The riparian vegetation was divided into five groups of Digitaria ciliaris colony group, Salix gracilistyla colony group, Erigeron annuus colony group, the group dominated by Humulus japonicus, Salix koreensis, Miscanthus sacchariflorus, and Phragmites japonica colonies, and the group dominated by Conyza canadensis and Echinochloa crusgalli var. echinata colonies. They had the similar health conditions. The CCA analysis showed that the environmental factors affecting the distribution of vegetation were physical factors such as vegetation area, artificial structure area, waterway area, branch width, channel width, and bank height and the biological factors such as the number of species. As such, it is necessary to maintain the health condition through continuous monitoring where the health condition is high and to apply active measures such as ecological restoration where the health condition is low.

Influence for a state of nutrition reached over Hb formation (영양상태(營養狀態)가 Hb 형성(形成)에 미치는 영향(影響))

  • Lee, Geum-Yeong;Jeon, Kwan-Bae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.4 no.1
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    • pp.13-17
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    • 1975
  • 1. A nursery school principal must think over physical stamina and health of his children, and he must make an epoch making changes to nutrition control. I think that authorities concerned must give weight to quality before quantity to manage the nursery school. If the nursery school principal who has aid received from abroad and his native country must manage the nursery school rationally. It is expected that, though the nutrition level is below the level of sports middle school children, it is above the common level of home children. 2. I fear that the calories which the pupils of sports middle school assimilate a day on the average is apt to be more calories as compared with other pupils of their age(weight). This is also true for their level of activity. It is required that the nutritionist must recheck the quantity of the calorie provision. 3. I took no thought about infection with parasites in this thesis. It is because that the pupils of sports middle school have much Hb (hemoglobin), because they have had meals with a settled menu for a year or two. So, that it is expected that it is caused only by the nutritious foods regardless of parasites and that they have little Hb(hemoglobin). Besides though they are little pupils, because they have much Hb. Of taking the level with upper life classes of the the Ewha Girls University students, it will be more reliable. Therefore as we maintain the states of health of the common home children and nursery school children normally, to increase the national power, the managers of nursery schools or the heads of families must pay attention to the control of nutrition, and authorities concerned must pay attention to the control of nutrition, and authorities concerned must take the trouble much more to the management of nursery school.1. We have been undertaking the occupations of the family plannings for more ten years. But we have still failed to realize the anticipated result in the farm villages. Though it is not in this district, I think that we must make efforts in these occupations urgently, and control increases in population, decrease the consumptions of food and salt, and contribute to the increasing of the general income. 2. There is a tendency to take a little more salted foods in the cities than in the farm villages. But we are apt to take more salt in quantity(20g) to be taken in a day both in the cities and in the farm villages than it is necessary to us. So it is required that we must grope for a means of increasing of the general income as early as possible, and that we must improve our diet to be got rid of dietary life in importance. And so we have to study throughly the side effect caused due to too much salt to be taken in, if it is discovered, we must spour on a means of sweep of it.

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Studies on the Overflow from Torrential Stream -A Case Study at the Samsung-cheon in Mt. Kwanak- (야계(野溪)의 월류발생(越流發生)에 관(關)한 연구(硏究) -관악산(冠岳山) 삼성천(三聖川)에서의 시험사례(試驗事例)-)

  • Woo, Bo Myeong;Kim, Kyong Ha;Jeong, Do Hyeon
    • Journal of Korean Society of Forest Science
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    • v.77 no.3
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    • pp.269-275
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    • 1988
  • To investigate the cause of overflow in the torrential stream, the estimated peak flow of run-off and the maximum tarring capacity of the stream were measured at the upstream of Samsung-cheon located in Kwanak Aboretum during July, 1987. The results obtained from this study could be summarized as follows : 1. The surveyed catchment area was 477ha, which was 116 of the designed area (410ha) by the plan. 2. The maximum rainfall intensity measured was 99.5mm/hr and was almost same as the designed intensity(100mm/hr). 3. The surveyed run-off coefficient was 0.672 that was about twice as much as designed one(0.35). 4. The surveyed peak flow of run-off was $88.59m^3/sec$, 222% as large the designed one($39.9m^3/sec$). 5. The designed cross-sectional area of the stream was $17.25m^2$, which was 68% of the designed one$25.43m^2$. 6. The surveyed hydraulic mean radius was 0.94m, which was shorter than the designed one(1.28m). 7. The surveyed mean stream-bed gradient(0.998%) was almost the same as the designed one(1.00%). 8 The surveyed maximum velocity of flow passing through the stream was 2.87m/sec, 78.0 of the designed one(3.68m/sec). 9 The surveyed run-off capacity of the stream was $49.51m^3/sec$, 53% of the designed one ($93.5m^3/sec$).

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Habitat Classification and Distribution Characteristic of Aquatic Insect Functional Feeding Groups in the Geum River, Korea (금강 수계 서식지 유형분류 및 수서곤충 섭식기능군 분포특성)

  • Park, Young-Jun;Kim, Ki-Dong;Cho, Young-Ho;Han, Yong-Gu;Kim, Yeong-Jin;Nam, Sang-Ho
    • Korean Journal of Environment and Ecology
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    • v.25 no.5
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    • pp.691-709
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    • 2011
  • This study was performed to classify habitat types depending on environmental factors and to find out distribution characteristics of functional feeding groups of aquatic insects which were collected at that habitat types. Field survey was conducted twice in a year for every spring and fall from 2007 to 2008 for 38 sites in the Geum River. During the field survey 15 environmental factors were measured at each 38 sites and analyzed by similarity analysis method to classify habitat types. The result of similarity analysis showed that the 38 sites could be grouped into 7 classes like as C1 and C3 class belong to Head water(HD), C2 and C4 and C5 class belong to Middle stream(MS), C6 and C7 class belong to Large River(LR) based on euclidean distances 4. And also, we could extract the main environmental factors affecting the classification of habitat types such as Stream Width and Elevation of physical environmental factors, Water Temperature, Conductivity and DO of chemical environmental factors, percentages of Sand, Silt and Gravel of substrate factors. Total 142 species of aquatic insects in 46 families, 9 orders were collected during the field surveys and the occurrence number of species and individuals showed high correlation with the Velocity factor and the percentage of Sand factor of each habitat types. In addition, correlation analysis between functional feeding groups and environmental factors represented that (1) Filtering-collectors(FC) affected by Velocity, Stream Width and Silt, (2) Gathering-collector(GC) affected by Velocity, (3) Predator(P) affected by Elevation, Velocity, Boulder, Conductivity and Sand, (4) Plant-piecer(PP) affected by Water Width and Silt, (5) Scraper(SC) affected by Elevation and Conductivity, (6) Shredder(SH) affected by Elevation, Boulder, DO, pH, Conductivity and Water Temperature respectively. As a result of this study, Elevation, Stream Width, Velocity, Conductivity, Water Temperature and percentage of Sand factors which were deduced by stepwise multiple regression analysis had correlations($r{\geqq}0.600$, p<0.01) with biota community inhabitation. Therefore these six environmental factors were regarded as major environmental factors that might affect highly the distribution of functional feeding groups in stream ecosystem of the Geum River.

Characteristics of Fish Fauna and Community Structure in Wangpicheon (왕피천 어류상 및 어류군집의 특성)

  • Hong, Yang-Ki;Kim, Kyeong-Hwan;Kim, Kyeong-Moo;Lim, Gwang-Ho;Song, Mi-Young;Lee, Wan-Ok
    • Korean Journal of Environment and Ecology
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    • v.30 no.5
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    • pp.874-887
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    • 2016
  • We surveyed bimonthly from April to October 2015 to understand the fish fauna and community structure in Wangpicheon. The collected species during the survey period were 40 species belonging to 15 families. Dominant species by number were Zacco koreanus (31.3%) and Rhynchocypris oxycephalus (14.7%). In biomass, the dominant species were Z. koreanus (29.2%) and Coreoperca herzi (8.6%). Also, nine endemic species (22.5%) including Squalidus multimaculatus and two endangered species (Lethenteron reissneri, Cottus koreanus) were identified. It was identified one introduced species (Oncorhynchus mykiss) from foreign countries for aquaculture. Compared with previous data, nine species were newly identified including L. reissneri, S. gracilis majimae, Pseudobagrus fulvidraco, O. mykiss, Chelon haematocheilus, Siniperca scherzeri, Acanthogobius lactipes, Luciogobius guttatus and Channa argus. Seven species (Rhodeus ocellatus, S. gracilis majimae, Hemibarbus longirostris, Pseudogobio esocinus, Microphysogobio yaluensis, Hemiculter eigenmanni and Cobitis hankugensis) introduced from other native waters in Korea were estimated. According to the analysis of the habitat characteristics of major migratory species (Tribolodon hakonensis, O. keta and Plecoglossus altivelis), O. keta was observed at station 11 and T. hakonensis at station 7, 10 and 11. These fishes have been mainly identified in the downstream. However P. altivelis was widely distributed from station 3 to 11. In the comparison of average standard length of P. altivelis at each station during the same period, populations collected from station 3 and 5 which are mid-upper area of the stream were $125.8{\pm}34.2mm$. Their growth was good compared with those collected from station 8 and 11 (mid-lower area): $80.2{\pm}16.6mm$. This difference in length comes from the artificial structures including weir, thus it is necessary to create a fishway that enables P. altivelis to pass around barriers for free movement and resource management.

Methodologies for Enhancing Immersiveness in AR-based Product Design (증강현실 기반 제품 디자인의 몰입감 향상 기법)

  • Ha, Tae-Jin;Kim, Yeong-Mi;Ryu, Je-Ha;Woo, Woon-Tack
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.44 no.2 s.314
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    • pp.37-46
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    • 2007
  • In this paper, we propose technologies for enhancing the immersive realization of virtual objects in AR-based product design. Generally, multimodal senses such as visual/auditory/tactile feedback are well known as a method for enhancing the immersion in case of interaction with virtual objects. By adapting tangible objects we can provide touch sensation to users. A 3D model of the same scale overlays the whole area of the tangible object so the marker area is invisible. This contributes to enhancing immersion. Also, the hand occlusion problem when the virtual objects overlay the user's hands is partially solved, providing more immersive and natural images to users. Finally, multimodal feedback also creates better immersion. In our work, both vibrotactile feedback through page motors, pneumatic tactile feedback, and sound feedback are considered. In our scenario, a game-phone model is selected, by way of proposed augmented vibrotactile feedback, hands occlusion-reduced visual effects and sound feedback are provided to users. These proposed methodologies will contribute to a better immersive realization of the conventional AR system.

Community Structure of Fish and Inhabiting Status of Natural Monument Cobitis choii in the Baekgok Stream, a Tributary of the Geum River Drainage Systrem of Korea (금강 지류 백곡천의 어류군집 및 천연기념물 미호종개 Cobitis choii의 서식양상)

  • Ko, Myeong-Hun;Hong, Yang-Ki;Kim, Hae-Lim;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.26 no.2
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    • pp.99-111
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    • 2014
  • Community structure of fish and inhabiting status of natural monument and endangered species, Cobitis choii were investigated in the Baekgok Stream, a tributary of the Geum River drainage system of Korea from May to November 2012. Ten-15 fish species inhabited upper stream (St. 1~St. 3) with a Aa or Aa-Bb river type composed of mostly pebble and cobble bottoms. 15~21 species did middle stream (St. 4~St. 5) with Aa or Aa-Bb type composed of mostly cobble, boulder and pebble bottoms. Nine species did Reservoir Baekgok (St. 6) with composed of mostly mud bottoms, and Seven species did Reservoir Baekgok outflow (St. 7) with Aa-Bb type composed of mostly boulder and cobble bottoms. And 20~23 species did lower stream (St. 8~St. 9) with a Bb-Bc type composed of mostly cobble and sand bottoms. A total of 35 species belonging to ten families were found in the stream during the survey. The dominant species were in the order of Zacco platypus (34.7%), Pungtungia herzi (9.4%) and Microphysogobio yaluensis (9.0%). Other abundant species included Tridentiger bifasciatus (8.2%), Pseudogobio esocinus (5.9%), Carassius auratus (4.6%), Squalidus chankaensis tsuchigae (3.7%), Rhinogobius brunneus (3.0%). Among residing species, ten (28.6%) species were endemic to Korea, one (Cobits choii) was endangered, and two (Micropterus salmoides and C. cuvieri) were non-indigenous. Natural monument and endangered species, C. choii were only inhabited St. 5, and they are inhabited 30~100 cm in water depth, sand bottom and slowly velocity. Using the mark-recapture method, populations were estimated at $7,838{\pm}6,290$ individuals. Age group in May estimated from total length indicated that the 38~45 mm group is 0 year old, the 46~60 mm group is 1 years old, the 61~80 mm group is 2 years old, and the 81~93 mm group is more than 3 years old. Condition factor (${\times}10^5) was 0.36~0.39 and 0.35~0.38 for female and male, respectively, and they are 13.5% were infected with parasites (digenea). We are discussed the health status, threats and conservation strategies of C. choii in the Baekgok Stream.