• Bulletin of Food Technology
• /
• v.15 no.2
• /
• pp.93-99
• /
• 2002

고압처리로 멸균된(high-pressure- sterilized) 저산성 식품은 아직 본격적으로 시장에 나오지는 않고 있다. 그러나 고압처리로 살균시켜(high-pressure-pasteurized) 제품의 부가가치를 높인 상품들은 일부 시판되고 있다. 미국시장에는 구아카몰(guacamole),굴 등이 있고, 일본과 유럽에는 잼, 젤리, 생선제품, 육제품, 슬라이스 햄, 샐러드 드레싱, 쌀떡, 주스, 요구르트 등이 판매되고 있다. 기술이 발전되어 처리비용이 떨어짐에따라 우유나 오렌지 주스 같은 저부가가치의 제품(high-volume commodity products)에도 고압처리 기술이 적용되는 예를 보게 될 것이다.미국 FDA의 "열처리된 저산성식품은 용접밀봉된 용기(캔, hermetically sealed container)에 포장되어야 한다." 는 규정(Title 21, Part 113 of the Code Federal Regulations)은 고압처리 식품을 염두에 두고 만들어진 규정은 아니지만, 저산성식품의 고압처리 공정(high-pressure processing)은 강력한 살균력을 제공하고 있다. 따라서 위의 FDA 규정(21 CFR 113)의 모든 조항은 고압처리 저산성 식품이 상업적인 생산에 들어가기 전에 시행되어야만 하는 것이다. 그 규정의 주요 조항중의 하나는 고압처리 공정에 대한 전문적인 지식을 가진 전문가나 위원회 등에 의하여 세부적인 고압처리공정 절차를 확립하는 것이다. 공정의 전문가들은 안전성을 증명하기 위해서 적합한 과학적인 방법을 사용하여 고압처리 공정을 확립해야만 한다. 저산성 식품에 대한 고압처리 공정은 주의깊게 설정된 공정조건하에서 재현성 있게 안전한 식품을 생산할 수 있어야 한다. 살균에 대한 안전성과 재현성이 입증되면 고압에 의한 살균처리공정 설계는 일반적인 대량생산 공정으로 활용될 수 있게 된다. PP(고압처리공정)의 유효성이 보다 확실하게 입증되고 널리 활용되려면, 공정의 살균력과 일관성(uniformity)을 증명하는 미생물적, 물리적, 화학적, 공학적인 여러 분야가 통합된 검증방법이 필요하다. 전통적인 열처리 공정은 스팀열의 가열시간으로 설명이 충분하다. 물론 HPP살균에서도 시간과 온도는 주요 변수이지만, 압력에 의한 미생물살균도 고려되어야만 한다. 또한 점도, 밀도, 구성성분, pH, 수분활성도 같은 해당제품의 특성도 제품의 고압처리 살균공정, 특히 미생물의 사멸에 영향을 미칠 수도 있다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2003.10a
• /
• pp.953-956
• /
• 2003

천연광물질인 투어멀린은 물분자를 만나면 수소(H$^{+}$)와 수산기(OH$^{-}$)로 분해하여 친수기와 소수기를 구분하여 발생하며, H$^{+}$와 OH$^{-}$는 각각 $H_2O$와 결합하여 활성이 강한 hydronium ion(H$_3$O$^{+}$)과 계면활성 작용이 있는 hydroxyl ion(H$_3$O$_{2-}$ )을 생성한다. 물속에서 불안정한 상태로 존재하는 수산기는 hydroxyl (-)ion을 형성하여 약 알카리성(pH-7.4)을 띄고, 물의 클러스터(cluster)를 세분화하는 수질 개선 기능과 함께 살균, 항균 및 세균번식억제 효과를 갖는다. 그러므로 투어멀린 전기석의 물 분해 효과를 이용하여 이것을 일정 시간 동안 증류수에 침적시킨 뒤, 그 침적수를 대장균이 배양된 액에 드롭시켜 대장균의 변화를 조사하였다. 그 결과, 시간이 경과됨에 따라 대장균 수의 변화가 나타남을 확인하였고, 따라서 투어멀린 침적수의 대장균 증식억제 효과를 확인하였다.

• Journal of Life Science
• /
• v.8 no.4
• /
• pp.421-425
• /
• 1998

The antifungal activity of polygodial, which was isolated from Polygonum hydropiper used as a hot spice in Japan, was investigated through measuring its MICs and against food-contaminants including 4 strains of Saccharomyces cerevisiae, 3 strains of Zygosaccharomyces species, and 5 strains of Aspergillus species. All yeast-like fungi exhibited strong antifungal susceptivilities with MICs and MFCs below 3.13${\mu}g$/ml. Strains of Aspergillu species showed moderate with MIC of 25-50${\mu}g$/ml and MFC of 50-100${\mu}g$/ml. However these strains to sorbic acid, as already known, revealed very weak with MICs of 200-800${\mu}g$/ml and MFCs above 1600${\mu}g$/ml. In addition, effects of various pHs and temperatures on the antifungal activity of polygodial were tested against S. cerevisiae. At $4^{\circ}C$, the fungicidal activity of polygodial gradually increased with incubation time and reached the maximum(MFC of 3. 13${\mu}g$/ml) in 5 hours. At temperatures in the range of 30-$45^{\circ}C$, the activity of polygodial increased in proportion to temperature rise and in particular at $45^{\circ}C$ was possible with the concentration of 0.1${\mu}g$/ml. mOn the other hand, medium pH was also identified to affect the antifungal activity of polygodial. Namely, compared to neutral (pH7), acidic(pH 3) and basic(pH 9) medium synergized its activity (MIC and MFC) to 8- and 2- fold, respectively.

• Microbiology and Biotechnology Letters
• /
• v.38 no.2
• /
• pp.177-182
• /
• 2010

The goal of this study was to characterize the quality and physiological functionality of some traditional rice wines in Chungnam province, Korea. Non-sterilized and commercial sterilized traditional rice wines from five traditional rice wine factories of Chungnam province were collected and investigated for nutritional components, noxious compounds and physiological functionality. Ethanol content ranged from 16.1~18.3% and pH ranged from 3.27~4.76, and they also contained 0.15% to 0.55% of total acid. All traditional rice wines contained 10.15~139.9 mg% of amino nitrogen and 2.5~25.7% of total sugar. Among organic acids, lactic acid was contained 7.4~29.6 mg%, and succinic acid and propionic acid was also contained 0.2~2.7 mg% and 0.7~8.3 mg%, respectively. Antihypertensive angiotensin I-converting enzyme inhibitory activity were showed 37.0~86.0% in all rice wines, however, fibrinolytic activity, antioxidant activity, SOD-like activity and acetylcholinesterase inhibitory activity were low or not detected.

• Applied Chemistry for Engineering
• /
• v.16 no.4
• /
• pp.570-575
• /
• 2005

In this study, we have manufactured electrodeless ultraviolet lamp which has a long life and a high degree of efficiency than the existing electrode UV lamp used in sewage effluent sterilization disinfection. First, we investigated change of UV intensity and temperature of lamp by activation materials. The best results for the dose response experiments were 250 minutes stabilizing to UV intensity of $300{\mu}W/cm^2$ and surface temperature $200{\sim}250^{\circ}C$ in Hg/Ind's weight ratio 95/5. When electrodeless UV lamp emits light for prolonged hours, surface temperature of lamp increases. therefore, temperature change is studied using a duplex lamp for cooling in actual sewerage process. Also, manufactured electrodeless UV lamp showed sterilization efficiency of more than 99.9% as result that experiment manufactured electrodeless UV lamp by E-coli. for sterilization disinfection of sewage effluent.

• Korean Chemical Engineering Research
• /
• v.56 no.4
• /
• pp.524-535
• /
• 2018

In this study, macroporous titania powders were synthesized utilizing the emulsion-assisted self-assembly to apply the removal of B. subtilis under UV irradiation, and the results were compared with the bactericidal effect of commercial titania nanoparticles. By changing the pore size of the porous titania powder, the reduction of B. subtilis by photocatalytic effect was measured, and the bactericidal capacity of the porous particles according to the pore size was compared in order to derive the optimum condition of the sterilization experiment. It was observed that the sterilization effect increased as the pore size became smaller, and it was confirmed that more than 50% of B. subtilis cold be removed for 1 hour of UV irradiation. Also, in order to promote the generation of active chemical species, a diluted solution of hydrogen peroxide was combined with the photocatalytic sterilization method, resulting in the removal of most of the strain after ultraviolet irradiation for 1 hour.

• Applied Chemistry for Engineering
• /
• v.25 no.4
• /
• pp.386-391
• /
• 2014

Atmospheric-pressure nonthermal corona discharge plasma was applied to the sterilization of biologically contaminated scoria powder. Escherichia coli (E. coli) culture solution was uniformly sprayed throughout the scoria powder for artificial inoculation, which was well mixed to ensure uniformity of the batch. The effect of the key parameters such as discharge power, treatment time, type of gas and electrode distance on the sterilization efficiency was examined and discussed. The experimental results revealed that the plasma treatment was very effective for the sterilization of scoria powder; 5-min treatment at 15 W could sterilize more than 99.9% of E. coli inoculated into the scoria powder. Increasing the discharge power, treatment time or applied voltage led to an improvement in the sterilization efficiency. The effect of type of gas on the sterilization efficiency was in order of oxygen, synthetic air (20% oxygen) and nitrogen from high to low. The inactivation of E. coli under the influence of corona discharge plasma can be explained by cell membrane erosion or etching resulting from UV and reactive oxidizing species (oxygen radical, OH radical, ozone, etc.), and the destruction of E. coli cell membrane by the physical action of numerous corona streamers.

• Applied Biological Chemistry
• /
• v.42 no.4
• /
• pp.356-360
• /
• 1999

Growth-inhibitory effects of citrus oils and agricultural fungicides, which were on the market, on several molds isolated from putrefied citrus fruits were investigated. When fungicidal activities of 11 kinds of synthetic agrochemicals against 6 species of molds, Alternaria alternata, Rhizopus sp., Botrytis cinerea, Monilia candida, Penicillium italicum and Penicillium digitatum, were investigated, agrochemicals containing mancozeb or fluazinam as an effective component had the broadest fungicidal spectrum. Agrochemicals containing iprodione, benomyl, azoxystrobin or thiophanate were less effective on the molds and those containing conazole derivative were intermediately effective. Resistance of the molds to the agrochemicals were species- and agrochemical-dependent. Among those molds tested, Penicillium italicum and Alternaria alternata showed relatively higher level of survival in the presence of synthetic fungicides. On the other hand, when the molds were exposed to citrus oils by direct contact, no cell could survive regardless of the species. The fungicidal activity of citrus oils was also confirmed by paper disk method and microscopic observation. These results suggested that citrus oils had broad killing activity against molds. Therefore, it would be necessary to design method for the application of citrus oils in order to improve post-harvest storage of citrus fruits.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2010.02a
• /
• pp.41-41
• /
• 2010

상온에 준하는 저온의 플라즈마를 발생시키는 장치들이 개발되면서, 저온 플라즈마와 생체조직간의 상호작용에 대한 연구가 큰 관심을 끌고 있다. 플라즈마에서 발생되는 다량의 이온과 활성종, 그리고 UV 등이 박테리아나 세포들과 작용함으로 해서 암세포 사멸, 치아 미백, 박테리아 살균/멸균, 지혈등의 효과들이 나타나고 있으며, 이러한 효과들을 극대화할 수 있는 장치 개발과 플라즈마와 생체조직간의 상호작용 메카니즘을 규명하는 것이 중요한 이슈가 되고 있다. 나노 금입자를 암세포의 막단백질인 FAK의 항체와 결합시킨 중합체를 만들어서, 암세포 표면에 나노 금입자붙이고, 플라즈마를 조사했을 때, 나노 금입자가 부착되지 않았을 경우에 비해서, 5배이상 사멸률이 증가하였다.[1] 변색된 치아에 미백제의 주성분인 과산화수소를 도포하고, 10분간 플라즈마를 조사하게 되면, 과산화수소만 도포했을 때에 비해, 치아 표면의 색이 3배이상 밝아지는 것을 관찰할 수 있었다. 과산화수소를 플라즈마에 노출시켰을 때, 활성종인 OH의 생성이 2배이상 증가하였고, 플라즈마에 의한 OH 생성의 촉진이 치아 미백효과가 증대되는 주된 요인인 것으로 추측된다.[2] 플라즈마에서 발생되는 O, $O_3$와 같은 활성종들은 살균력이 뛰어나기 때문에, 저온 플라즈마를 의료기구의 소독/멸균에 응용할 가능성이 아주 크다. 대장균이나 구강 세균이 플라즈마 처리로 5분이내에 멸균되는 것을 확인하였고, 핸드피스와 같은 의료기구를 오염시켜서 멸균 테스트를 수행하고 있다.

• The Korean Journal of Pesticide Science
• /
• v.10 no.4
• /
• pp.249-261
• /
• 2006

Fifty compounds such as ester, sulfonyl ester, carbamate, ether and phosphoyl ester derivatives of 4-isopropyl-3-methylphenol(I) and 5-isopropyl-3-methylphenol(II) were synthesized. These derivatives were identified by IR, GC/MS and $^1H$-NMR spectra. Their in vitro antifungal activities were tested against 10 plant pathogenic fungi. Among them, several compounds showed potent in vitro antifungal activity. The selected compounds showing potent in vitro antifungal activity were tested for their in vivo antifungal activities against 5 plant diseases such as rice blast, rice sheath blast, cucumber anthracnose, cucumber gray mold and tomato late blight. As a result, 4-isopropyl-3-methylphenyl(2-amino-thiazole-4-yl)methoxyiminoacetate(I-7a) showed a potent in vivo antifungal activity against rice blast. Both methyl (4-isopropyl-3-methylphenoxy)acetate(I-4d) and methyl (5-isopropyl-3-methylphenoxy)acetate(II-4d) effectively inhibited the development of cucumber gray mold.