• Korean Journal of Clinical Laboratory Science
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• v.47 no.4
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• pp.243-250
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• 2015

A bone marrow biopsy that has undergone decalcification with 10% nitric acid could not be used for various pathological tests and had extremely limited reproducibility. The fixing solution of each experimental group was differentiated in usage, one solution including acid and the other not. The use of the decalcification solution was separated into either acidic or alkaline (EDTA), and further experiments were conducted with differing time phases. When using a fixing solution and decalcification solution which included acid, the specimens were faulty to the extent that all pathological tests were impossible. However specimens that were processed with an EDTA type decalcification solution did not display a non-specific reaction in EBV ISH and were even able to produce results that were at a level suited to various studies or a pathological diagnosis in the FISH, DNA, RNA tests. By improving the fixing and decalcification of bone marrow biopsy, the study was able to make possible ISH, FISH, DNA tests as well as RNA study, and secured the sensitivity, specificity, and reproducibility of various test methods. The stabilization of various test methods that use bone marrow biopsy contributes to the diagnosis, prognosis, prediction, treatment of the patient and provide guidelines for decision-making.

• Restorative Dentistry and Endodontics
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• v.32 no.1
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• pp.47-60
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• 2007

The purpose of this study is to compare and to evaluate the effect of pH and lactic acid concentration on the progression of artificial root caries lesion using polarizing microscope, and to evaluate the morphological changes of hydroxyapatite crystals of the demineralized area and to investigate the process of demineralization using scanning electron microscope. Artificial root caries lesion was created by dividing specimens into 3 pH groups (pH 4.3, 5.0, 5.5), and each pH group was divided into 3 lactic acid concentration groups (25 mM, 50 mM, 100 mM). Each group was immersed in acid buffer solution for 5 days and examined. The results were as follows : 1. Under polarized microscope, the depth of lesion was more effected by the lactic acid concentration rather than the pH. 2. Under scanning electron microscope, dissolution of hydroxyapatite crystals were increased as the lactic acid concentration increased and the pH decreased. 3. Demineralized hydroxyapatite crystals showed peripheral dissolution and decreased size and number within cluster of hydroxyapatite crystals and widening of intercluster and intercrystal spaces as the pH decreased and the lactic acid concentration increased. 4. Under scanning electron microscope evaluation of the surface zone, clusters of hydroxyapatite crystals were dissolved, and dissolution and reattachment of crystals on the surface of collagen fibrils were observed as the lactic acid concentration increased. 5. Under scanning electron microscope, demineralizatlon of dentin occurred not only independently but also with remineralization simultaneously. In conclusion, the study showed that pH and lactic acid concentration influenced the rate of progression of the lesion in artificial root caries. Demineralization process was progressed from the surface of the cluster of hydroxyapatite crystals and the morphology of hydroxyapatite crystals changed from round or elliptical shape into irregular shape as time elapsed.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.27 no.2
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• pp.7-13
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• 1997

조직학적으로 유용성이 입증된 산탈회법을 이용한 인접면 비교적 초기 치아 우식의 병소를 형성하여 진단율을 조사하였다. 산 용액을 이용하여 20개 인접면 치아우식을 20개 소구치에 형성하였고, 37개 인접면 치아우식을 30개 대구치에 형성하였다. 건전한 소구치 20개, 대구치 30개를 포함하여 총 96개 치아를 4개씩 나누어 24개의 블록을 형성하였고, 각각 2개 블록의 교합면을 교합시켜서, 교익촬영을 하였다. 촬영 결과를 36명의 치과의사들이인접면 치아우식의 유무를 기록하고, 동시에 및 ROC 분석을 위한 5 개 범주의 판독 기준으로 판독하여 기록하였다. 인접면 치아우식증 유, 무만으로 판독한 결과 진단의 sensitivity는 0.71, specificity는 0.78 이였다. ROC 분석 한 결과의 곡선도표 아래부분의 평균 면적은 약 0.806 이였다. 치아우식증 유무만으로 진단한 결과는 특정한 sensitivity와 specificity 만을 나타내지만, ROC 분석 결과는 주관적 진단 기준과 구별되는 고유의 진단 능력을 표시하는 1-specificity(False Positive)의 변화에 따른 sensitivity(True Positive)의 변화를 연속적으로 나타내어 주었다.

• Restorative Dentistry and Endodontics
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• v.35 no.2
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• pp.96-105
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• 2010

The purpose of this study is to observe and compare the remineralization tendencies of artificial enamel caries lesion by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel). For this study, 48 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 2 days in order to produce artificial dental caries that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffering remineralization solution of four different degrees of saturation (0.507, 0.394, 0.301, and 0.251) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (${\times}100$). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were est mated. While the group with the lowest degree of saturation (0.251) showed total remineralization feature from the surface to the subsurface of the lesion, the group with the highest degree of saturation (0.507) showed demineralization mainly on the surface of the lesion at the constant organic acid concentration 0.01 M and pH 5.5.

• The korean journal of orthodontics
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• v.25 no.2 s.49
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• pp.165-174
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• 1995

The purpose of this study was to evaluated the improve method of demineralization and damage on the enamel stufece related to bonded orthodontic bracket. Enamel surface of the 40 Intact premolars were treated by aicd etching and various fluoride application methods and then maintained in the patient mouth of 3 months. After extraction of all the sampled premolars, enamel surfaces were evaluated by Scanning electron microscope. The obtained results were as follow. 1. Enamel surface relate to bonded orthodontic bracket showed demineralization and damage, slightly. 2. Group 6 treated by $2\%$ NaF iontophoresis presented and almost similarity to nomral enamel surface. 3. Group 2 treated by acid etching had demineralization and damage on the enamel surface much more than other groups treated by various fluoride application methods. 4. Demineralization and damage on the enamel surface caused by bonded orthodontic bracket is improved by various fluoride application methods.

• Journal of the korean academy of Pediatric Dentistry
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• v.29 no.1
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• pp.76-83
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• 2002

The purpose of study was to determine the effectiveness of crude IgY to S. mutans in preventing the acid production and the demineralization of primary tooth enamel in vitro. The acid production by S. mutans in Todd Hewitt broth with and without 5% sucrose was inhibited by 2.5% crude IgY, and as the concentration of crude IgY increased from 2.5% to 17.5%, the pH drop of the media after incubation continued to decrease. There were high positive correlations between the concentration of crude IgY and the pH of media in the late incubation period. The inhibition rate of demineralization of primary tooth enamel by S. mutans was determined by measuring the surface microhardness after incubation in 5% sucrose Todd Hewitt broth for 12 hours. The inhibition rate was 32.28% in 2.5% IgY, 42.28% in 7.5% IgY, 64.06% in 12.5% IgY, and 92.79% in 17.5% IgY. There was high positive correlation between the concentration of crude IgY and the surface microhardness of enamel after demineralization These results suggest that it would be possible to prevent dental caries through passive immunization using crude IgY.

• Restorative Dentistry and Endodontics
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• v.34 no.6
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• pp.526-536
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• 2009

The aim of this vitro-study is to evaluate the effects of fluoride on remineralization of artificial dentine caries. 10 sound permanent premolars, which were extracted for orthodontic reason within 1 week. were used for this study. Artificial dentine caries was created by using a partially saturated buffer solution for 2 days with grounded thin specimens and fractured whole-body specimens. Remineralization solutions with three different fluoride concentration (1 ppm. 2 ppm and 4 ppm) were used on demineralized-specimens for 7 days. Polarizing microscope and scanning electron microscope were used for the evaluation of the mineral distribution profile and morphology of crystallites of hydroxyapatite. The results were as follows: 1. When treated with the fluoride solutions, the demineralized dentine specimens showed remineralization of the upper part and demineralization of the lower part of the lesion body simultaneously. 2. As the concentration of fluoride increased, the mineral precipitation in the caries dentine increased. The mineral precipitation mainly occurred in the surface layer in 1 and 2 ppm- specimens and in the whole lesion body in 4 ppm -specimens. 3. When treated with the fluoride solution, the hydroxyapatite crystals grew. This crystal growth was even observed in the lower part of the lesion body which had shown the loss of mineral.

• Restorative Dentistry and Endodontics
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• v.33 no.5
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• pp.481-492
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• 2008

The purpose of this study is to observe and compare the remineralization tendencies of artificially demineralized enamel by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel). For this study, 36 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 3 days in order to produce dental caries artificially that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffered remineralization solution of three different degrees of saturation (0.25, 0.30, 0.35) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope $({\times}100)$. The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were determined. In conclusion, in the group with the lowest degree of saturation, remineralization occurred thoroughly from the surface to the subsurface lesion, whereas in the groups with greater degree of saturation showed no significant change in the subsurface lesion, although there was corresponding increase in the remineralization width on the surface zones.

• Journal of Periodontal and Implant Science
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• v.33 no.2
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• pp.311-319
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• 2003

치주조직재생을 도모하기 위한 전통적인 시술방법으로는 여러 가지 골이식재를 이용한 골이식술이 오래 전부터 이용되고 있다. 이번 실험의 목적은 장,단기간의 신생골의 형성과 CPP의 흡수를 비교하여 골이식재로서 Calcium Polyphosphate(CPP)를 평가하는 것이다. 이번 실험에 사용된 CPP는 무수 $Ca(H_2PO_4)$를 condensation하여 무결정의 $Ca(PO_3)_2$를 얻고 이를 용융하고 냉각시킨후 분쇄하여 얻은 것으로 키토산이나 $Na_2O$를 첨가한 후 3세된 비글견에 이식하여 관찰하였다. 양성 대조군으로 동결탈회건조골을 이용하였다. 조직학적으로 3개월 소견과 같이 섬유조직의 게재없이 신생골의 형성이 관찰되었다. 12개월 후의 신생골의 형성은 3개월 결과에 비해서 동결탈회건조골이나 키토산, $Na_2O$를 넣은 CPP 과립에서 더 많은 비율로 나타났다. 음성 대조군과 이식재를 넣은 군간에는 유의성이 있는 것으로 나타났고(p<0.05), 또한 키토산을 첨가한 CPP 과립과 $Na_2O$를 첨가한 CPP 과립 사이에는 신생골의 형성에 유의성이 없었다. 이식한 CPP 과립의 경우 흡수소견이 3개월 결과에 비해 크게 증가하지 않았다. 이번 실험에서는 첨가물에 상관없이 CPP 과립은 골유도성과 생체적합성을 보였다. 그러나 흡수속도가 매우 느려 신생골로 대체되는 여부를 알 수 없었다. 향후 연구에서는 흡수속도를 증가시킬 수 있는 적당한 CPP 형태와 첨가물을 밝혀내어야 할 것이다.

• Journal of Periodontal and Implant Science
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• v.31 no.3
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• pp.543-554
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• 2001

치주조직 재생을 도모하기 위한 전통적인 시술방법으로 여러가지 골이식재를 이용한 골이식술이 이용되고 있다. 이번 실험의 목적은 골형성을 위한 재료로 Calcium Polyphosphate(CPP)를 사용하여 비글견에서의 조직 반응과 골유도성을 보는 것이고 또한 다른 이식재들간에 신생골의 형성에 어느정도 영향을 주는 가를 알아보는 것이다. 이번 실험에 사용된 CPP는 무수 $Ca(H_2PO_4)$를 condensation하여 무결정의 $Ca(PO_3)_2$를 얻고 이를 용율하고 냉각시킨 후 분쇄하여 얻은 것으로 3세된 비글견에 이식하여 관찰하였다. 조직학적으로 CPP granule의 경우는 키토산이나 $Na_2O$를 넣은 경우 모두 bone 의 ingrowth가 관찰되었고 다른 섬유조직의 개재를 볼수 없었다 동결탈회건조골을 넣은 경우에는 주위로 골형성 보이지 않았고 단지 섬유성 조직이 관찰 되었다. 아무것도 넣지 않은 경우에 비해서 동결탈회건조골이나 키토산, $Na_2O$를 넣은 CPP granules 경우에 더 많은 비율로 신생 골의 양이 나타나는 것을 볼수 있었다. 아무것도 넣지 않은 대조군과 이식재를 넣은 군간에는 유의성이 있는 것으로 나타났고(p<0.05). 또한 CPP granules with chitosan과 CPP granules with $Na_2O$ 사이에는 신생골의 형성에 유의성이 없었다. 이것으로 보아 CPP granules with chitosan과 CPP granules with $Na_2O$는 모두 골유도성이 있고 신생골의 형성을 촉진하므로 치주질환으로 인한 골결손 부위에 사용할 수 있는 재료로 우수한 특성을 지닌다고 사료된다.