• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.5
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• pp.461-466
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• 1991

For the purpose of improving storability and process adaptability of potato, we carried out an experiment to investigate the effects of microwave heating on the total lipid content and fatty acid composition of the potatoes in storage. The samples were made by powdering potatoes exposed to the microwave energy in a 560W, 2,450MHz oven for 0, 60, 120 and 180 seconds respectively. From the examination of the samples stored at th temperature of $25{\pm}1^{\circ}C$ for 12 months, the following results were obtained. As an over-all tendency, the longer the samples were heated and the longer they were stored, the total lipid content decreased but the microwave-heated samples showed decreasing rates less than those of the controls for the whole storage periods. As major fatty acids of the potato flour, linoleic acid (30.92 %), palmitic acid (29.34 %), linolenic acid (8.90 %) and stearic acid (8.23 %) were detected from the samples. The longer the samples were exposed with microwave, unsaturated fatty acids like 18:2, 18:3 increased. generally with the lapse of storage time, saturated fatty acids increased while unsaturated ones decreased. But in the samples heated with microwave, we observed an apparent suppression of the increasing rate of the saturated fatty acids and the decreasing rate of the unsaturated fatty acids, and the suppressing effects turned out to be proportional to the length of microwave heating.

• Journal of Nutrition and Health
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• v.9 no.1
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• pp.104-109
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• 1976

Condiments such as welsh onion, garlic, ginger, black pepper, red pepper, japanese pepper, mustard, horse radish, monosodium glutamate and sugar were ground by a homogenizer, and 0%, 1%, 5%, and 10% of each of the ground condiments were put into 0.2% ${\alpha}-amylase$ solution for storage at the temperature of $15^{\circ}C$. ${\alpha}-amylase$ activity then was measured by the Wohlgemuth method at 48-hour interval, and the following results were obtained. 1) Among the condiments, black pepper and sugar checked the ${\alpha}-amylase$ activity most, about 80% in comparison with control. 2) Welsh onion, garlic, mustard, and ginger checked the ${\alpha}-amylase$ activity, about 50% in comparison with control, irrespective of the time stored. 3) The low concentration of red pepper, horse radish, japanese pepper and mono-sodium glutamate checked the ${\alpha}-amylase$ activity about 50%, while the high one did below 75% in comparison with control. To conclude: all the condiments used in the experiment checked ${\alpha}-amylase$ activity.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.499-502
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• 2007

The study was performed to assess the antinociceptive efficacy of Korean bee venom(BV) in the rat formalin test. Korean BV was collected using BV collector devices in which an electrical impulse is used to stimulate the worker bee(Apis mellifera L.) to sting and release venom. After collection, whole Korean BV was evaporated until dry using BV collector. Experiments were performed on male Sprague-Dawley rats(weighing 260-270, 6 weeks old). Rats divided into 4 groups, each comprising 8 rats. BV was diluted and amounts of 6 mg/kg body weight(BW), 0.6 mg/kg BW and 0.06 mg/kg BW were tested. BV was subcutaneously injected to produce an antinociceptive effect and the antinociceptive efficacy was evaluated using a rat formalin test. BV was subcutaneously injected into an acupoint(Zusanli, ST36) at 15 min prior to 1% formalin($50{\mu}l$) injection. The antinociceptive effect observed during 60 min following formalin administration. BV produced antinociceptive efficacy from 10 to 60 min after formalin injection. The antinociceptive efficacy of Korean BV showed a dose-dependent response. These results suggest that Korean BV may be a suitable and preferred choice for antinociceptive efficacy in pain management.

• Applied Biological Chemistry
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• v.38 no.4
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• pp.345-352
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• 1995

The influence of vacuum cooling and modified atmospheric packaging was investigated about browning degree, polyphenoloxidase(PPO) activity and free amino acid of shiitake mushroom. During storage, surface browning was inhibited by precooling. PPO activities of shiitake mushroom was increased during storage. Especially, PPO activity was rapidly increased as surface browning was proceeded. And PVC-wrapped mushroom was lower than carton box-packed mushroom in the changes of surface browning and PPO activity. Total free amino acid contents of shiitake mushroom was 2,510 mg% at harvest, but free amino acid content of shiitake mushroom decreased consistantly during storage. Precooled mushroom had more free amino acid content than non-precooled and their contents were fluctuated by storage temperature, packaging methods and storage periods.

• Korean Journal of Weed Science
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• v.17 no.4
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• pp.421-430
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• 1997

This experiment was conducted to detect the presence of allelopathic substances in the leaves of Ginkgo biloba L. Water extracts from G. biloba leaves which collected at different season markedly inhibited the germination and growth of O. sativa, E. crus-galli, D. sanguinalis, and L. sativa, indicating the presence of biological substances. Linolenic and palmitic acid were the major fatty acids of G. biloba leaves. The biochemical substances such as salicylic arid, p-coumaric acid, catechol, hydroquinone, orchinol, ferulic acid, phloroglucinol, and umbelliferone etc., belonging to the phenolic, compounds were, detected in a large amount, which may be responsible for exhibition inhibitory effects. The common phenolic compounds were detected in the early-harvested and late-harvested G. biloba leaves were salicylic and p-coumaric acid. All these compounds were related to the allelopathic activities in G. biloba leaves.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.186.1-186
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• 2003

온주밀감의 당도를 높이고 신선도를 유지하기 위하여 무가온 비닐하우스에서 이듬해 2-4월에 수확하는 월동감귤은 부피과의 발생이 쉽고 저장성이 나빠 수확시기에 바로 출하하는 감귤을 제외하고는 저온저장이 필수적이다. 2월 하순에 수확한 월동감귤(궁천조생)을 저장 전에 키토산과 칼슘처리를 하고 저장에 미치는 영향을 검토하였다. 부패과 발생은 저장 30일부터 나타났고, 무처리에서는 그 이후 부패가 빠르게 진행되었다. 그러나 베푸란 또는 키토산과 칼슘 처리에서는 저장 60일까지 부패과의 발생을 억제할 수 있어서, 이 기간까지가 저장이 가능한 기간으로 보인다. 중량감소는 11월 하순에 수확한 궁천조생에 비하여 모든 처리에서 중량감소가 빠르게 진행되었다. 유리당은 11월에 수확한 궁천조생과 비교하였을 때 glucose은 비슷하였으나, sucrose, fructose은 다소 많은 양이 검출되었다. 유기산은 citric acid과 malic acid 2종류가 검출되었으며, 그 함량도 관행수확기에 수확한 감귤보다 citric acid는 1/2 이하로 낮게 나타났고, malic acid는 비슷한 함량이 나타났다. 저장기간이 경과함에 따라 유기산 함량은 서서히 감소하였으나, malic acid은 초기에 감소되었으나 3월 중순이후에는 일정한 수준을 나타났다. 유리아미노산의 함량은 11월에 수확한 궁천조생에 비하여 D,L-$\alpha$-aminoadipic acid, $\alpha$-amino- $\beta$-guanidinopropionic acid 등 2종을 제외한 27종이 검출되었다.

• Journal of Life Science
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• v.26 no.10
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• pp.1130-1136
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• 2016

The purpose of this study was to investigate the anti-proliferative and apoptotic effects of acid extract of Gracilaria verrucosa (AEG) on RC-58T/h/SA#4 primary human prostate cancer cells. AEG significantly decreased the cell viability of prostate cancer cells in a dose-dependent manner. AEG also showed relatively low cytotoxicity on normal cell (RWPE-1). The morphology of prostate cancer cells treated with AEG was distorted to shrunken cell masses. In addition, it was revealed that AEG induced cell death as evidenced by increased formation of apoptotic body and nuclear condensation. Furthermore, AEG clearly modulated the down regulation of Bcl-2 (anti-apoptotic)/Bax (pro-apoptotic) family and activated caspase-3 as an effector caspase in a dose-dependent manner. AEG inhibited cell proliferation induced by environmental hormones as a bisphenol A in a dose-dependent manner. These results indicate that AEG act as anti-proliferative effects as a potential therapeutic agent on primary human prostate cancer cells.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.10
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• pp.1395-1401
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• 2012

This study investigated the cognition-related effects on antioxidant activities, ${\gamma}$-aminobutyric acid (GABA) contents, and AChE inhibitory activities in ethanol extracts from Zingiber officinale Rosc. (Korea), Curcuma longa L. (Korea), Curcuma longa L. (Myanmar), and Curcuma aromatica Salisb. (India). These extracts were investigated to determine the relationships among total polyphenols as well as DPPH and ABTS radical scavenging activities, GABA contents, and acetylcholinestrase (AChE) inhibitory activity. For the results, C. longa L. from Myanmar exhibited the highest contents of curcumin (97.1 ${\mu}g/mg$), total polyphenols (98.4 ${\mu}g/mg$), and GABA (1.31 ${\mu}g/mg$), as well as the strongest radical scavenging activities and AChE inhibitory activity. In addition, C. aromatica Salisb from India, which had the highest total polyphenol content (98.4 ${\mu}g/mg$) and strongest radical scavenging activities, exhibited relatively high AChE inhibitory activity similar to that of C. longa L. from Myanmar. On the other hand, Z. officinale Rosc. and C. longa L. from Korea showed low contents of curcumin (12.2 ${\mu}g/mg$) and polyphenols (85.7 ${\mu}g/mg$), as well as low AChE inhibitory activities. However, we could detect 1.11 ${\mu}g/mg$ of GABA in these extracts, which was similar to that of C. longa L. from Myanmar. Therefore, GABA content was not correlated with AChE inhibitory activity. Based on the results, AChE inhibitory activity is highly correlated with polyphenol contents in Zingibearceae family. Overall, among the Zingiberaceae tested, C. longa L. from Myanmar might be a strong candidate as a cognitive-enhancing ingredient.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.302-302
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• 1994

오메프라졸의 산 불안정성을 개선하기 위하여 신규 합성한 오메프라졸 cholestyramin resin 염 복합체와 오메프라졸 hydroxypropyl-$\beta$-cyclodextrin (HP-$\beta$-CD) 포접화합물의 약효를 검색하였다. 생체외 실험 (in vitro test)으로 H$^{+}$/K$^{+}$-ATPase 활성도 저해효과를 검토하였으며 생체내 실험 (in vivo test)으로 Shay 결찰법에 의한 위산분비 억제효과에 대한 실험을 실시하였다. 오메프라종 염 복합체와 오매프라졸 포접화합물은 1$\times$$10^{-5}$-1$\times$$10^{-3}$M농도 범위에서 용랴의존적으로 H$^{+}$K$^{+}$-ATPase 활성을 억제시켰으며 $IC_{50}$/치는 오메프라졸 결과와 유사하였다. 셍체네 실험에서는 오메프라졸 HP-$\beta$-CD 포접화합물이 오메프라졸과 그 resin염복합체보다 위액분비량, 펩신 활성도에 대한 $IC_{50}$/치가 낮았으며 이는 생체내에서 포접 화합물이 오메프라졸의 안정성을 증가시킴으로서 위산분비 억제효과를 증가시킨것으로 사료된다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1992.05a
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• pp.42-42
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• 1992

ras oncogene은 암조직이나 transformed human cell line에서 거의 공통적으로 발견되는 oncogene으로서 그 product인 p2l 단백질은 C-terminal 25개의 아미노산 외에는 거의 동일한 배열을 가지고 있는 매우 conservative한 단백질이며 C-terminal cysteine이 carboxy methylation되어 있고 또한 palmitic acid와 같은 long chain fatty acid도 결합되어 있다. 보고된 바에 의하면 p21 protein의 palmitation은 ras protein의 세포막에 대한 친화력을 유지시키며 이와 같은 친화력은 cell transforming activity의 기본요건으로 알려져 있다. 이와 같은 관점에서 볼때 p21 단백질의 C-terminal processing현상을 new drug target으로, 즉 p2l 단백질의 C-terminal processing을 억제하므로서 cell transforming activity를 저해 할 수 있을 것이므로 생체내에 존재하는 p21 단백질 C-terminal processing 억제제의 identification 및 purification은 항암제 연구와 밀접한 관계가 있다. 구체적으로 farnesyl-protein transferase inhibitor 혹은 carboxyl methyl inhibitor의 identification 및 purification은 이같은 목적을 달성 할 수 있는 가능성이 크다.