• Title/Summary/Keyword: 산화방지

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Improvement of anti-oxidant and anti-inflammatory activities of aleurone layer extracts of black rice (Oryza sativa L.) by enzyme treatment (효소 처리에 의한 흑미 호분 추출물의 산화방지와 항염증 활성 증진)

  • Lee, Mi Kyoung;Ryu, Soo In;Lee, Min Ho
    • Korean Journal of Food Science and Technology
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    • v.50 no.5
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    • pp.528-534
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    • 2018
  • The current study investigated the effects of enzyme treatment on black rice (Oryza sativa L.) aleurone layer extracts. Different enzymes (lipase, lecitase ultra and lipopan 50BG) were used to test anti-oxidant and anti-inflammatory activities in vitro. The antioxidant activities of enzyme treated or non-enzyme treated extracts of black rice bran were evaluated via 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity. Lipase treated extracts of black rice bran showed higher antioxidant activity compared to that of non-enzyme treated extracts. Anti-inflammatory activities of enzyme treated black rice bran extracts on nitrite production and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) secretion, were tested using a nitric oxide (NO) colorimetric assay kit and an enzyme-linked immunosorbent assay (ELISA) kit. The ethanolic extract of enzyme treated black rice bran decreased the levels of nitric oxide production and pro-inflammatory cytokines, such as tumor necrosis $factor-{\alpha}$, in a lipopolysaccharidestimulated RAW 264.7 cell culture. These findings indicate that enhanced anti-oxidant and anti-inflammation activities of the ethanolic extracts of enzyme treated black rice (Oryza sativa L.) aleurone layers, may be attributed to molecular conversion of ingredients in enzyme catalyzed reactions.

Antioxidant Effects of Phenolic Acids and Ginseng Extract in Aqueous System (수용성 계에서 Phenolic Acid류와 인삼 추출물이 갖는 산화방지 효과)

  • Lee, Hyung-Ok;Park, Ock-Jin
    • Korean Journal of Food Science and Technology
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    • v.30 no.2
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    • pp.434-438
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    • 1998
  • The antioxidant effects of 700 ppm ginseng extract, 100ppm caffeic acid, ferulic acid, vanillic acid, or ${\alpha}-topherol$ on the 1% linoleic acid aqueous buffer system was studied by measuring malondialdehyde (MDA) and headspace oxygen. The compounds showed antioxidant activities in the following order: $caffeic\;acid{\geq}ferulic\;acid\;>\;{\alpha}-tocopherol$>ginseng extract>vanillic acid, with the oxidation inhibition ratio of 63.5, 62.9, 52.3, 51.2 and 5.6% of the control according to MDA results. The mixture of 100 ppm caffeic acid, 100 ppm ${\alpha}-tocopherol$ and 700 ppm ginseng extract had a high oxidation inhibition ratio of 91.2%. Headspace oxygen results had a similar trend with the MDA results. Headspace oxygen results showed that the antioxidant activities were in order of ferulic acid>caffeic acid>${\alpha}-tocopherol$>ginseng extract>vanillic acid and headspace oxygen contents were 18.56, 17.78, 17.17, 16.65 and 15.95%, respectively.

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Antioxidant and anti-inflammatory effects of the ethyl acetate fraction of the Agastache rugosa extract (배초향 에틸아세테이트 분획의 산화방지 및 항염증 활성)

  • Kim, Bomin;Han, Yeong Eun;Lee, Hwa Jin
    • Korean Journal of Food Science and Technology
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    • v.49 no.3
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    • pp.331-337
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    • 2017
  • To evaluate the antioxidant activity of hexane, ethyl acetate, and butanol fractions obtained from Agastache rugosa extract, we measured the total polyphenol levels, DPPH radical scavenging activity, and reducing power. The ethyl acetate fraction of A. rugosa (AREA) displayed high phenolic levels, potent DPPH radical scavenging effect, and powerful reducing power. In addition, we examined the ability of AREA to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-activated BV-2 microglia. AREA suppressed NO production and inducible nitric oxide synthase (iNOS) expression and downregulated interleukin-6 (IL-6) mRNA level in LPS-stimulated BV-2 microglia. Furthermore, we detected rosmarinic acid in AREA by HPLC, which suggested that rosmarinic acid could be one of the bioactive materials responsible for the antioxidant and anti-inflammatory activities of AREA. These results suggested that AREA may be a good source of functional foods with antioxidant and anti-inflammatory activities.

Antioxidant activity and inhibitory effect of melatonin and the relative indole compounds on perilla oil oxidation (멜라토닌 및 관련 인돌 화합물의 산화방지능과 들기름 산화에 대한 억제 효과)

  • Kim, Seok Joong
    • Korean Journal of Food Science and Technology
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    • v.48 no.6
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    • pp.610-617
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    • 2016
  • Melatonin, known as a powerful wide-spectrum antioxidant, is consumed as a food supplement in some countries, but its applicability as an antioxidant additive was not yet studied. Therefore, we evaluated the antioxidant activity of melatonin by DPPH, ABTS, FRAP and ORAC assays as well as its ability to inhibit perilla oil oxidation. The activities of four other related indoles were also compared. Melatonin showed the highest antioxidant activity (mmol trolox equivalent per mol indole, mmol TE) in ORAC (2,159) assay, but a low antioxidant activity in DPPH (0.63), ABTS (91), and FRAP (764) assays, whereas serotonin showed an opposite result. Addition of 1% (w/w) melatonin to perilla oil extended the induction period of oxidation up to about 2 times ($2.93{\pm}0.47h$) compared to that of control ($1.43{\pm}0.26h$) in the Rancimat assay, corresponding to almost 50% of the ability of butylated hydroxyl toluene (BHT). Tryptamine was the most effective indole that inhibited perilla oil oxidation ($9.53{\pm}1.43h$).

In Vitro Antioxidative Activity and Polyphenol and Tocopherol Contents of Bugak with Lotus Root, Dried Laver, or Perilla Leaf (연근, 김, 깻잎 부각의 산화방지 활성, 폴리페놀 화합물과 토코페롤 함량)

  • Jung, Leejin;Song, Yeongok;Chung, Lana;Choe, Eunok
    • Korean journal of food and cookery science
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    • v.30 no.6
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    • pp.767-773
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    • 2014
  • In vitro antioxidative activities and antioxidants of the lotus root, dried laver, and perilla leaf bugak, Korean traditional fried cuisine, were evaluated. The bugak was prepared with fermented glutinous rice batter and unroasted sesame oil for use in the pan-frying. The perilla leaf bugak showed the highest radical scavenging activity and reducing power, with the lowest in the lotus root bugak. The antioxidative activity of the blanched lotus root was lower than the dried laver or the perilla leaf. Polyphenol content was higher in the perilla leaf bugak than the lotus root or the dried laver bugak, and tocopherols were mainly derived from frying oil. The antioxidative activity of bugak was correlated well with polyphenol contents, but there was no correlation between tocopherol contents and the antioxidative activity.

Changes in color stability and antioxidant properties of dietary pigments after thermal processing at high pressures (고온가압 처리에 의한 식용색소의 화학안정성 및 산화방지활성 변화)

  • Oh, Boeun;Kim, Kunhee;Hong, Jungil
    • Korean Journal of Food Science and Technology
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    • v.54 no.3
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    • pp.257-263
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    • 2022
  • Various dietary pigments are added to processed foods to improve their sensory and commercial properties. In this study, autoclave sterilization (121℃ for 15 min at 15 psi) was performed on 34 food pigments, and changes in their color stability and antioxidant activity were analyzed. The autoclaving process drastically reduced the peak color intensities of water-soluble paprika and beet red (BR) by ~90%. Turmeric oleoresin (TO), water-soluble β-carotene, and grape skin color were also unstable and showed a remaining color intensity of 45-60%. The colors of all the synthetic pigments tested were stable under this process. The scavenging activities of BR and paprika against ABTS, DPPH, and AAPH radicals decreased significantly, whereas those of TO were enhanced after the autoclaving treatment. The results suggest that the chemical and bioactive properties of certain dietary pigments are affected by the autoclaving process, and this phenomenon should be considered during food processing.

Numerical Flow Analysis for Anti-Vortex Device (AVD) in Oxidizer Tank (산화제 탱크의 와류방지장치 유동해석)

  • Jang, Je-Sun;Han, Sang-Yeop;Kil, Gyoung-Sub;Cho, In-Hyun
    • Aerospace Engineering and Technology
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    • v.9 no.2
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    • pp.168-175
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    • 2010
  • During feeding oxidizer to the engine, vortices are occurred at lower dome of oxidizer tank inside by various working environments and external forces for liquid propellant feeding system of space launch vehicle. To eliminate the vortices or swirls Anti-Vortex Devices(AVD) shall be installed at inside lower oxidizer tank. Using the numerical analysis, we have confirmed the performance of AVD and analyzed the mass flow rate by feeding time and magnitudes of swirls on the free surface of oxidizer or exit surface according to the AVD number and length. Then we could derive the optimal design of the AVD number and length.

Lipid oxidation and antioxidant mechanisms in different matrix (매질(matrix)에 따른 지방산화 및 산화방지능 메커니즘)

  • Yi, BoRa;Kim, Mi-Ja;Lee, JaeHwan
    • Food Science and Industry
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    • v.51 no.2
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    • pp.127-135
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    • 2018
  • The action of antioxidants was different depending on the environments where antioxidants were located. Although basic mechanisms of lipid oxidation and antioxidants were related each other, their contribution on the degree of oxidation was different. In thisreview, terminology on antioxidant properties were defined such as antioxidant activity and antioxidant capacities. In addition, antioxidant mechanisms including primary and secondary antioxidants or hydrogen donating or electron transferring antioxidants were introduced. Also, the impact of physical points of view and antioxidant polar paradox were introduced. Depending on the types of food matrice including bulk oil, oil-in-water emulsion (O/W), or solid state, antioxidant actions showed different degree and this point was explained in detail.

Factors affecting passivation of Cu(Mg) alloy film (Cu(Mg) alloy의 산화방지막 형성에 영향을 미치는 인자)

  • 조흥렬;조범석;이원희;이재갑
    • Journal of the Korean Vacuum Society
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    • v.9 no.2
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    • pp.144-149
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    • 2000
  • Variables affecting the passivation capability of Cu(Mg) alloy films, which were sputter deposited from a Cu (4.5 at. %) target, have been investigated. The results show that the passivation capability of a Cu(Mg) alloy film is a function of annealing temperature, $O_2$ pressure, and Mg content in the film. Increasing the annealing temperature up to $500^{\circ}C$ favors formation of a dense MgO layer on the surface which has a growth limited thickness of 150 $\AA$. Decreasing the $O_2$ pressure enhances the preferential oxidation of Mg over Cu. Furthermore, increasing the Mg content in the Cu(Mg) film promotes formation of a dense MgO layer. Vacuum pre-annealing was found to be very effective in segregating Mg to the surface, facilitating the passivation capability of the Cu(Mg) alloy film even when the Mg content is low. In the current study, self-aligned MgO layers with low resistivity and an effective passivation capability over the Cu surface, have been obtained by manipulating these factors when Cu(Mg) thin films are annealed.

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Effect of antioxidant treatment in axillary culture medium of walnut(Juglans sinensis) (호두 액아 배양시 산화방지제 처리 효과)

  • Kwon, Young Hee;Lee, Joung Kwan;Park, Jae Seong;Huh, Yoon Sun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.38-38
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    • 2018
  • 최근 호두 기내 소비량은 급증하나 대부분 실생묘로 조림되어 결실까지 장기간 소요되며, 무성 번식률이 낮아 보급확대가 어렵다. 본 연구는 호두나무 관행 묘목 번식법 개선이 가능한 우량 국산묘 대량증식 기술 개발로 최적 배양조건을 구명하고자 수행하였디. 호두나무 등 목본류는 식물 조직배양 기본배지에서 산화방지제를 첨가하여 사용하는 경우, 배양체에서 생기는 페놀 화합물(Phenolic compound)의 작용을 억제하여 배양 식물체의 갈변(Browning) 또는 흑변(Blackening) 현상을 감소시키는 효과가 있다. 페놀 추출물은 배지로 빠져나와 갈변시키며 생존율을 저하시키는 원인이 되고 이를 제거하는 산화방지제는 배양 식물체의 생산효율을 높이는 효과를 나타내는 강력한 항산화제(Antioxidant) 역할을 한다. 호두나무의 액아를 배양하게 되면 조직 및 배지의 갈변화가 심하게 일어나는데, 휴면 정도가 깊을 수록 정단에서 추출되는 페놀성 물질의 함량이 증가하며, 식물 조직 내 페놀성 물질의 함량 정도와 배양의 고사율과는 상관관계가 있다. 이러한 식물 배양체에서 생기는 페놀 화합물에 의해 발생되는 배양상의 문제점을 해결하기 위하여 계대배양을 자주해 주거나 활성탄(Activated charcoal), Ascorbic Acid 등의 첨가물을 배지에 추가하는 방법이 있다. 이에 따라 호두 '신령' 품종의 액아배양의 페놀집적을 제거하기 위해 배지 내에 산화방지제 첨가물질 등을 농도별로 처리하였다. 호두나무 액아 배양 후 8주차 산화화방지제 첨가물질의 농도별 처리 효과는 생존율은 85.3%를 보였으며, 신초 형성율 76.4%, 신초수 1.9개/주, 신초장 27mm/주의 양호한 결과를 나타냈으며, 페놀 제거 및 유식물체 고사 억제 효과를 보였다.

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