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http://dx.doi.org/10.9721/KJFST.2017.49.3.331

Antioxidant and anti-inflammatory effects of the ethyl acetate fraction of the Agastache rugosa extract  

Kim, Bomin (Department of Natural Medicine Resources, Semyung University)
Han, Yeong Eun (College of Pharmacy, Sookmyung Women's University)
Lee, Hwa Jin (Department of Natural Medicine Resources, Semyung University)
Publication Information
Korean Journal of Food Science and Technology / v.49, no.3, 2017 , pp. 331-337 More about this Journal
Abstract
To evaluate the antioxidant activity of hexane, ethyl acetate, and butanol fractions obtained from Agastache rugosa extract, we measured the total polyphenol levels, DPPH radical scavenging activity, and reducing power. The ethyl acetate fraction of A. rugosa (AREA) displayed high phenolic levels, potent DPPH radical scavenging effect, and powerful reducing power. In addition, we examined the ability of AREA to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-activated BV-2 microglia. AREA suppressed NO production and inducible nitric oxide synthase (iNOS) expression and downregulated interleukin-6 (IL-6) mRNA level in LPS-stimulated BV-2 microglia. Furthermore, we detected rosmarinic acid in AREA by HPLC, which suggested that rosmarinic acid could be one of the bioactive materials responsible for the antioxidant and anti-inflammatory activities of AREA. These results suggested that AREA may be a good source of functional foods with antioxidant and anti-inflammatory activities.
Keywords
Agastache rugosa; antioxidant activity; nitric oxide; anti-inflammatory; rosmarinic acid;
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1 Shin S, Kang CA. Antifungal activity of the essential oil of Agastache rugosa Kuntze and its synergism with ketoconazole. Lett. Appl. Microbiol. 36: 111-115 (2003)   DOI
2 Kim JB, Kim JB, Cho KJ, Hwang YS, Park RD. Isolation, identification, and activity of rosmarinic acid, a potent antioxidant extracted from Korean Agastache rugosa. J Korean Soc. Agri. Chem. Biotechnol. 42: 262-266 (1999)
3 Wang X, Perumalsamy H, Kwon HW, Na YE, Ahn YJ. Effects and possible mechanisms of action of acacetin on the behavior and eye morphology of Drosophila models of Alzheimer's disease. Sci. Rep. 5: 16127 (2015)   DOI
4 Cho HI, Park JH, Choi HS, Kwak JH, Lee DU, Lee SK, Lee SM. Protective mechanisms of acacetin against D-galactosamine and lipopolysaccharide-induced fulminant hepatic failure in mice. J. Nat. Prod. 77: 2497-2503 (2014)   DOI
5 Vincent L, Chen W, Hong L, Mirshahi F, Mishal Z, Mirshahi-Khorassani T, Vannier JP, Soria J, Soria C. Inhibition of endothelial cell migration by cerivastatin, an HMG-CoA reductase inhibitor: Contribution to its anti-angiogenic effect. FEBS Lett. 495: 159-166 (2001)   DOI
6 Cho HI, Hong JM, Choi JW, Choi HS, Kwak JH, Lee DU, Lee SK, Lee SM. Beta-Caryophyllene alleviates D-galactosamine and lipopolysaccharide-induced hepatic injury through suppression of the TLR4 and RAGE signaling pathways. Eur. J. Pharmacol. 764: 613-621 (2015)   DOI
7 Moon H, Kim MJ, Son HJ, Kweon HJ, Kim JT, Kim Y, Shim J, Suh BC, Rhyu MR. Five hTRPA1 agonist found in indigenous Korean mint, Agastache rugosa. PLoS One. 10: e0127060 (2015)   DOI
8 Oh HM, Kang YJ, Lee YS, Park MK, Kim SH, Kim HJ, Seo HG, Lee HG, Lee JH, Chang KC. Protein kinase G-dependent heme oxygenase-1 induction by Agastache rugosa leaf extract protects RAW 264.7 cells from hydrogen peroxide-induced injury. J. Ethnopharmacol. 103: 229-235 (2006)   DOI
9 Kim HK, Lee HK, Shin CG, Huh H. HIV integrase inhibitory activity of Agastache rugosa. Arch. Pharm. Res. 22: 520-523 (1999)   DOI
10 Rani V, Deep G, Singh RK, Palle K, Yadav UC. Oxidative stress and metabolic disorders: pathogenesis and therapeutic strategies. Life Sci. 148: 183-193 (2016)   DOI
11 Rivest S. Regulation of innate immune responses in the brain. Nat. Rev. Immunol. 9: 429-439 (2009)   DOI
12 Li L, Lu J, Tay SS, Moochhala SM, He BP. The function of microglia, either neuroprotection or neurotoxicity, is determined by the equilibrium among factors released from activated microglia in vitro. Brain Res. 1159: 8-17 (2007)   DOI
13 Lue LF, Kuo YM, Beach T, Walker DG. Microglia activation and anti-inflammatory regulation in Alzheimer's disease. Mol. Neurobiol. 41: 115-128 (2010)   DOI
14 Uddin R, Saha MR, Subhan N, Hossain H, Jahan IA, Akter R. Alam A. HPLC-analysis of polyphenolic compounds in Gardenia jasminoides and determination of antioxidant activity by using free radical scavenging assays. Adv. Pharm. Bull. 4: 273-281 (2014)
15 Aziz MA. Qualitative phytochemical screening and evaluation of anti-inflammatory, analgesic and antipyretic activities of Microcos paniculata barks and fruits. J. Integr. Med. 13: 173-184 (2015)   DOI
16 Quideau S, Deffieux D, Douat-Casassus C, Pouysegu L. Plant polyphenols:chemical properties, biological activities, and synthesis. Angew. Chem. Int. Ed. Engl. 50: 586-621 (2011)   DOI
17 Padda MS, Picha DH. Methodology optimization for quantification of total phenolics and individual phenolic acids in sweetpotato (Ipomoea batatas L.) roots. J. Food Sci. 72: C412-C416 (2007)   DOI
18 Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200 (1958)   DOI
19 Jasprica I, Bojic M, Mornar A, Besic E, Bucan K, Medic-Saric M. Evaluation of antioxidative activity of Croatian propolis samples using DPPH* and $ABTS*^{+}$ stable free radical assays. Molecules 12: 1006-1021 (2007)   DOI
20 Manach C, Williamson G, Morand C, Scalbert A, Remesy C. Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am. J. Clin. Nutr. 81: 230S-242S (2005)   DOI
21 Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16: 144-158 (1965)
22 Bray HG, Thorpe WV. Analysis of phenolic compounds of interest in metabolism. Meth. Biochem. Anal. 1: 27-52 (1954)
23 Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghsakadbi SS, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. J. Assoc. Physicians India 52: 794-804 (2004)
24 Alfadda AA, Sallam RM. Reactive oxygen species in health and disease. J. Biomed. Biotechnol. 2012: 936486 (2012)
25 Khurana S, Piche M, Hollingsworth A, Venkataraman K, Tai TC. Oxidative stress and cardiovascular health: therapeutic potential of polyphenols. Can. J. Physiol. Pharmacol. 91: 198-212 (2013)   DOI
26 Hu C, Kitts DD. Luteolin and luteolin-7-o-glucoside form dandelion flower suppress iNOS and COX-2 in RAW 264.7 cells. Mol. Cell Biochem. 265: 107-113 (2004)   DOI
27 Jhee OH, Yang CB. Antioxidant activity of extract from Bangah herb. Korean J. Food Sci. Technol. 28: 1157-1163 (1996)
28 Moncada S, Palmer RM, Higgs EA. Nitric oxide:physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 43: 109-142 (1991)
29 Geller DA, Billiar TR. Molecular biology of nitric oxide synthases. Cancer Metastasis Rev. 17: 7-23 (1998)   DOI
30 Lee HJ, Li H, Chang HR, Jung H, Lee DY, Ryu JH. (-)-Nyasol, isolated from Anemarrhena asphodeloides suppresses neuroinflammatory response through the inhibition of I-kBa degradation in LPS-stimulated BV-2 microglial cells. J. Enzyme Inhib. Med. Chem. 28: 954-959 (2013)   DOI
31 Domitrovic R, Skoda M, Vasiljev Marchesi V, Cvijanovic O, Pernjak Pugel E, Stefan MB. Rosmarinic acid ameliorates acute liver damage and fibrogenesis in carbon tetrachloride-intoxicated mice. Food Chem. Toxicol. 51: 370-378 (2013)   DOI
32 Lee HJ, Lim HJ, Lee DY, Jung H, Kim MR, Moon DC, Kim KI, Lee MS, Ryu JH. Carabrol suppresses LPS-induced nitric oxide synthase expression by inactivation of p38 and JNK via inhibition of I-kappa B alpha degradation in RAW 264.7 cells. Biochem. Biophys. Res. Commun. 391: 1400-1404 (2010)   DOI
33 Zhao F, Chen L, Zhang M, Bi C, Li L, Zhang Q, Shi C, Li M, Zhou S, Kong L. Inhibition of lipopolysaccharide-induced iNOS and COX-2 expression by indole alkaloid, 3-(hydroxymehtyl)-6,7-dihydroindolo[2,3-a]quinolizin-(12H)-one, via NF-${\kappa}B$ inactivation in RAW 264.7 macrophages. Planta Med. 79: 782-787 (2013)   DOI
34 Erkan N, Ayranci G, Ayranci E. Antioxidant activities of rosemary (Rosmarinus officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem. 110: 76-82 (2008)   DOI
35 Huang N, Hauck C, Yum MY, Rizshsky L, Widrlechner MP, McCoy JA, Murphy PA, Dixon PM, Nikolau BJ, Birt DF. Rosmarinic acid in Prunella vulgaris ethanol extract inhibits lipopolysaccharide-induced prostaglandin E2 and nitric oxide in RAW 264.7 mouse macrophages. J. Agric. Food Chem. 57: 10579-10589 (2009)   DOI
36 Fallarini S, Miglio G, Paoletti T, Minassi A, Amoruso A, Bardelli C, Brunelleschi S, Lombardi G. Clovamide and rosmarinic acid induce neuroprotective effects in in vitro models of neuronal death. Br. J. Pharmacol. 157: 1072-1084 (2009)   DOI
37 Zhu F, Asada T, Sato A, Koi Y, Nishiwaki H, Tamura H. Rosmarinic acid extract for antioxidant, antiallergic, and ${\alpha}$-glucosidase inhibitory activities, isolated by supramolecular technique and solvent extraction from Perilla leaves. J. Agr. Food Chem. 62: 885-892 (2014)   DOI
38 Zielinska S, Matkowski A. Phytochemistry and bioactivity of aromatic and medicinal plants from the genus Agastache (Lamiaceae). Phytochem. Rev. 13: 391-416 (2014)   DOI
39 Xu Y, Jiang Z, Ji G, Liu J. Inhibition of bone metastasis from breast carcinoma by rosmarinic acid. Planta Med. 76: 956-962 (2010)   DOI
40 Lee BY, Hwang JB. Physiochemical characteristics of Agastache rugosa O. Kuntze extract by extraction conditions. Korean J. Food Sci. Technol. 32: 1-8 (2000)
41 Ahn B, Yang CB. Volatile flavor components of Bangah (Agastache rugosa O. Kuntze) herb. Korean J. Food Sci. Technol. 23: 582-586 (1991)
42 Li HQ, Liu OZ, Liu ZL, Du SS, Deng ZW. Chemical composition and nematicidal activity of essential oil of against Agastache rugosa Meloidogyne incognita. Molecules 18: 4170-4180 (2013)   DOI
43 Haiyan G, Lijuan H, Shaoyu L, Chen Z, Ashraf MA. Antimicrobial, antibiofilm and antitumor activities of essential oil of Agastache rugosa from Xinjiang, China. Saudi. J. Biol. Sci. 23: 524-530 (2016)   DOI
44 Shin S. Essential oil compounds from Agastache rugosa as antifungal agents against Trichophyton species. Arch. Pharm. Res. 27: 295-299 (2004)   DOI