• Journal of Life Science
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• v.24 no.7
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• pp.777-783
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• 2014

Cathepsin D (CtsD), an aspartyl peptidase, is involved in apoptosis, resulting in the release of cytochrome C from mitochondria in cells. Here, we investigated microRNA regulation of CtsD expression in 3T3-L1 cells. First, we observed the expression of CtsD in cells in response to doxorubicin (Dox). As expected, the level of CtsD mRNA increased in 3T3-L1 cells exposed to Dox in a dose-dependent manner. The cellular viability of ectopically expressed CtsD cells was decreased. Next, we used the miRanda program to search for particular microRNA targeting CtsD. MiR-145 was selected as a putative controller of CtsD because it had a high mirSVR score. In a reporter assay, the luciferase activity of cells containing the CtsD 3'-UTR region decreased in cells transfected with a miR-145 mimic compared to that of a control. The level of CtsD expression was down-regulated in preadipocytes ectopically expressing miR-145 and up-regulated by an miR-145 inhibitor. Cells also suppressed miR-145 expression when exposed to Dox. The miR-145 inhibitor reduced the cellular viability of 3T3-L1 cells. Taken together, these data suggest that miR-145 regulates CtsD-mediated cell death in adipocytes. These findings may have valuable implications concerning the molecular mechanism of CtsD-mediated cell death in obesity, suggesting that CtsD could be a useful therapeutic tool for the prevention and treatment of obesity by regulating fat cell numbers.

• Resources Recycling
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• v.14 no.5 s.67
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• pp.40-44
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• 2005

The purified phosphoric acid was recovered from crude phosphoric acid with high contents of aluminium and molybdenum ions to reuse the acid as an etchant. In this work, solvent extraction was applied to recover the phosphoric acid from crude phosphoric acid. Phosphate was used as an extractant. Further cleaning and removing processes on the recovered phosphoric acid were conducted to eliminate the metallic ion impurities in the acid. The process parameters were successfully optimized, so that the finally purified acid contained less than 1 ppm of aluminium and molybdenum ion.

• Journal of Life Science
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• v.23 no.9
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• pp.1170-1176
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• 2013

Essential oils have been used to treat psychophysiological disorders, but their effects on human electroencephalographic (EEG) activity have not been thoroughly investigated. We evaluated the effects of essential oil of Zizyphus jujuba seeds, a Korean folk medicine known as San-Jo-In, on human EEG activity. For this purpose, essential oil was extracted from San-Jo-In by the supercritical carbon dioxide extraction method. The effect of its inhalation on EEG activity was evaluated by measuring the EEG power spectrum (25 indices) in 20 healthy participants. The results of the EEG power spectrum indicated that the values of the theta wave decreased significantly (p<0.05) in the left (from 17.277 to $13.854{\mu}V$) and right parietal (from 15.324 to $13.020{\mu}V$) regions compared to the other regions. During the inhalation of San-Jo-In oil, the EEG spectrum values of fast alpha, relative gamma, and spectral edge frequency increased 50% compared to those before inhalation. The values of the fast alpha wave increased significantly (p<0.05) in the left prefrontal (from 0.063 to $0.085{\mu}V$), right prefrontal (from 0.064 to $0.085{\mu}V$), and left frontal (from 0.073 to $0.100{\mu}V$) regions following inhalation of the San-Jo-In essential oil. The changes in the EEG activities following inhalation of San-Jo-In suggest that the oil can improve psychological well-being by increasing attention and relaxation.

• Proceedings of the Korean Institute of Resources Recycling Conference
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• 2005.05a
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• pp.168-172
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• 2005

초산, 질산 및 인산이 함유된 폐혼산에서 초산과 질산을 1차 분리하고 남은 조인산으로부터 정제인산으로 회수한 후 에칭액의 원료로 재활용하기 위하여 용매 추출 기술을 검토하였다. 알루미늄(Al) 및 몰리브덴(Mo) 불순물이 함유된 조인산을 인산염 추출제를 이용하여 인산을 추출한 후 세정공정과 탈거공정을 거쳐 알루미늄과 몰필브덴을 분리하기 위한 최적 분리조건을 도출하고자 하였다. 실험 결과 추출공정과 세정공정, 그리고 탈거 공정을 통하여 알루미늄 및 몰리브덴의 함량을 1ppm이하로 분리 제거하여 정제 인산으로 회수가 가능하였다.

• YAKHAK HOEJI
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• v.26 no.3
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• pp.133-138
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• 1982

• Journal of Oriental Neuropsychiatry
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• v.11 no.2
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• pp.79-86
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• 2000

This study has been designed and performed to identify the effect on insomnia patients according to th injection of herbal medicine induced from Sanjoincho.The result of the injection shows as follows.1. Total duration of sleeping time showed 1.1500${\pm}$1.5433 hours of increase. (p〈0.01, respectively)2. Delaying time before the onset of sleep showed 0.8158${\pm}$1.3251 hours of increase in sleep onset insomnia(p〈0.05, respectively)3. The wake times showed 1.375${\pm}$1.4079 times of decrease in sleep maintenance isomnia. (p〈0.05, respectively)

• Food Science of Animal Resources
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• v.25 no.1
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• pp.20-25
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• 2005

This study was conducted to evaluate the effects of Zizyphus vulgaris supplementation on growth performance, blood cortisol and meat quality characteristics in finishing pigs. The total of thirty-six [Duroc${\times}$Yorkshir${\times}$Landrace] pigs ($91{\pm}2.11$ kg average initial body weight) were used in a 30-days assay. Dietary treatments included 1) CON (basal diet), 2) T1 (basal diet for 15 days and 0.1 % Zizyphus vulgaris for 15 days) and 3) T2 (0.1 % Zizyphus vulgaris for 30 days). The ADG (Average daily gain), ADFI (Average daily feed intake) and ADG/ADFI during the feeding period were not significantly differences among the treatments (p>0.05). Backfat thickness of pigs fed CON was higher than those of T1 and T2 (p<0.05). The appearance rate of A or B carcass grade was in T1 (74%) and T2 (84%) was significantly higher than that in CON (58%) (p<0.05). Pigs fed Zizyphus vulgaris 0.1 % for 30 days tended to decrease on blood cortisol compared with pigs fed CON and T1. But, there was not significantly difference among the treatments (p>0.05). The Hunter's L/sup */ (lightness) value of loin in the pork fed CON was higher than that of loin in the pork fed T1 and T2 (p<0.05). After 7 days, the L/sup */ value of loin in the pigs fed T2 was higher increased than that of pigs fed T1 and CON (p<0.05). However, a/sup */ and b/sup */ values were not affected by dietary Zizyphus vulgaris (p>0.05). There were not found remarkable differences in sensory properties (marbling, firmness and color) among the treatments. The results from the present study suggest that Zizyphus vulgaris could be a effective feed additive to improve meat quality of pigs. However, further research is needed to investigate effects of carcass characteristics.

• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.35-41
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• 1998

Essential amino acid composition of seventy eight medicinal herbs marketed in Korea was analyzed of the basis of their dried weight. From the results analyzed, it was shown that Alisma orisntale (S.) J. (1,911.5 mg%), Foeniculum vulgare M. (1,090.8 mg%), Artemisia princeps P. (1,073.6 mg%) and Typha latifolia L.(956.1 mg%) contain in threonine. Alisma orisntale (S.) J. (2,523.3 mg%), Nelumo nucifera G. (1,984.7 mg%) and Foeniculum vulgare M. (1,553.8 mg%) contain in lysine. Nelumo nucifera G. (738.3 mg%), Alisma orisntale (S.) J. (635.2 mg%) and Foeniculum vulgare M. (507.7 mg%) contain in methionine. Nelumo nucifera G. (2,496.9 mg%), Alisma orisntale (S.) J. (2,295.6 mg%) and Coix lachrymajobi L. var. M. S. (2,222.5 mg%) contain in leucine. Nelumo nucifera G. (1,624.6 mg%), Alisma orisntale (S.) J. (1,432.7 mg%) and Xantnium strumarium L. (1.371.7 mg%) contain in isoleucine. Alisma orisntale (S.) J (1,791.0 mg71), Nelumo nucifera G. (1,570.6 mg%) and Xantnium strumarium L. (1,498.7 mg%) contain in phenylalanine.

• KOREAN POULTRY JOURNAL
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• v.38 no.6 s.440
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• pp.187-190
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• 2006

한국양계TS(주)-승진인사단행/ (주)경축 HACCP인증 획득 및 신입사원 신규채용/ 해훈케미칼(주)-'YTN-TV, MBN-TV, 생활 · 건강-TV' 취재, 촬영/ (주)동방-파워 · 프로텍션 시리즈 출시/ (주)한펠-고객 초청 세미나 & 창립 13주년/ (주)CKF-사료공장 준공/ 대일축산기계-사무실 이전, 전화번호 변경/ 마니커-(주)아성과 업무제휴 조인식 체결/ 산골농장-제6회 장미축제 개막

• Korean Journal of Biological Psychiatry
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• v.4 no.1
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• pp.54-59
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• 1997

Objects : Sanjoin, the seeds of Zizyphus vulgaris var. spinosus has been used as the most important hypnotic agent in chinese medicine to treat insomnia. This research was performed in order to examine the effect of betulinic acid and sanjoinine-A which are components of Sanjoin. Method : Sleeping time, sleep recordings of EEG, EMG, serum serotonin level, and locomotor activity were measured in rats which received betulinic acid and sanjoinine-A as sleep induction material extracted from Sanjoin. Results : 1) Groups received betulinic acid, sanjoinine-A, and lorazepam showed increased sleep time than control group with saline. 2) Groups with betulinic acid, sanjoinine-A, lorazepam and saline recorded ${\beta}$-wave in sleep recordings of EEG. In EMG, there was no significant difference among all groups. 3) No significant difference in serum serotonin level among all groups was found. 4) In autonomic activity testing, groups of betulinic acid, sanjoinine-A, and lorazepam showed significantly more decreased in activity than saline group. In comparison of groups of betulinic acid and sanjoinine-A with a group of lorazepam, there was no significant difference. Conclusion : These results suggest that betulinic acid and sanjoinine-A have the sedative effect like lorazepam rather than sleep effect.