• Title/Summary/Keyword: 산소 요구도

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Quantification of White Spot Syndrome Virus (WSSV) in Seawaters Using Real-Time PCR and Correlation Analyses between WSSV and Environmental Parameters (Real-Time PCR을 이용한 해수 존재 흰반점 바이러스의 정량 및 양식 환경인자와의 상관관계 분석)

  • Song, Jae-Ho;Choo, Yoe-Jin;Cho, Jang-Cheon
    • Korean Journal of Microbiology
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    • v.44 no.1
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    • pp.49-55
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    • 2008
  • White Spot Syndrome Virus (WSSV) is one of the most virulent viral agents in the penaeid shrimp culture industry. In this study, WSSV in a Fenneropenaeus chinensis shrimp farm and an adjacent seawater were concentrated using a membrane filtration and quantified using the quantitative real-time PCR (QRT-PCR) method with newly designed primers and Taqman probe. Sensitivity of primers and probe was proven by WSSV standard curve assay in QRT-PCR. In order to demonstrate the relationship between WSSV and environmental parameters, physicochemical and biological parameters of the farm and influent seawaters were monitored from June to September, 2007. The abundance of WSSV ranged 3,814-121,546 copies per 1 liter of seawater, which was correlated with fecal enterococci ($r^2=0.9$, p=0.02), chlorophyll ${\alpha}$ ($r^2=0.8$, p=0.03) and $BOD_5$ ($r^2=0.8$, p=0.07). Subsequently, it is concluded that the QRT-PCR method using Taqman probe established in this study was efficient to clarify the quantification of WSSV in seawaters. Statistical analyses of environmental parameters obtained in this study also showed that the abundance of WSSV was correlated with several biological parameters rather than physicochemical parameters.

Zooplankton Community and Distributions of Copepods in Relation to Eutrophic Evaluation in Chinhae Bay (진해만 수질 환경과 동물플랑크톤 군집 및 요각류 분포 특성)

  • KANG Young-Shil;PARK Joo-Suck;LEE Sam-Seuk;KIM Hak-Gyoon;LEE Phil-Yong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.4
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    • pp.415-430
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    • 1996
  • Spatio-temporal variations in zooplankton community and ropepod indicator species were investigated along with the interaction between zooplankton distribution and environmental factors in Chinhae Bay. Zooplankton samples were monthly collected at 7 stations from February to September in 1993. A NORPAC net was vertically hauled from bottom to surface, At the same station, environmental factors such as temperature, salinity and COD (chemical oxygen demand) were measured at two different water layers, surface and bottom. In August and September, salinity declined below 30.00‰ , while eutrophic parameters such as COD showed the higher concentrations than those in other months, with higher concentrations at inner bay stations. Salinities were, however, higher at bay mouth areas. These distributional patterns were believed to be caused by input and dispersion of organic matters from nearby land. Zooplankton communities were composed of 7~14. Of these, Noctiluca scintillans was predominant and occupied 90.6‰ of total zooplankton abundance. Cladocera and Copepoda were secondly abundant taxa. Among 6 to 10 copepod species appeared, Acartia omorii and A. hudsonics were most common species during the survey months except March and September. Species diversities were greater, in general, at inner bay than outer bay. The lowest diversity index was observed in February, while the highest in July. Cluster analysis could divide the study area into 2 or 4 zones for each month. Zone 1, mouth area of the bay, was characterized by the influence of offshore waters. Zone II was mixing area. Zone III and IV seemed to be affected by nearby land.

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A Study on Combustion Characteristics in terms of the Type of Fuel Supply Device (Feeder) of a Wood Pellet Boiler (목재펠릿보일러의 연료공급 장치의 형태에 따른 연소특성에 관한 연구)

  • Choi, Yun Sung;Euh, Seung Hee;Oh, Kwang Cheol;Kim, Dae Hyun;Oh, Jae Heun
    • Journal of Energy Engineering
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    • v.24 no.2
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    • pp.120-128
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    • 2015
  • This study reports the combustion characteristics, such as burner temperature and the concentration of exhausted gas ($O_2$, $CO_x$, $NO_x$) due to the different types and pitches of the fuel supply feeder of the wood pellet boiler. The 1st grade wood pellets composed of mainly larch have been used for the experiment. In case of using the spring feeder, mean temperature of burner was approximately $821.76^{\circ}C$, and the mean concentration of oxygen, carbon monoxide, carbon dioxide and nitrogen oxide were approximately 8.88%, 93.35ppm, 12.15% and 139.83 ppm, respectively. The test result with the spring feeder was shown to approach the condition of complete combustion compared to that of a screw feeder and were in good agreement with authentication judgement standard. Furthermore, the combustion efficiency was improved according to the growth of screw pitch. The control of air flow rate from the blower and ventilator is needed to achieve the complete combustion.

Effect of Acute High-intensive Swimming Exercise on Blood Electrolytes and Metabolites (단기간 고강도의 수영운동이 혈액 이온 및 대사산물에 미치는 영향)

  • Kim, Shang-Jin;Park, Hye-Min;Shin, Se-Rin;Jeon, Seol-Hee;Kim, Jin-Shang;Kang, Hyung-Sub
    • Journal of Veterinary Clinics
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    • v.27 no.3
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    • pp.262-267
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    • 2010
  • Magnesium ($Mg^{2+}$) is an essential co-factor for over 325 physiological and biochemical processes so that plays a central role of neuronal activity, cardiac excitability, neuromuscular transmission, muscular contraction, vasomotor tone, and blood pressure significantly related to physical performance. However, only limited information on blood ionized $Mg^{2+}$ ($iMg^{2+}$) regarding to physical exercise is available and the data from blood total $Mg^{2+}$ detection are inconsistent. This present study investigated the changes of blood $iMg^{2+}$ correlated with metabolic demands during acute high-intensive exhaustive physical exercise in rats. After exhausted swimming (3-4 hours), blood pH, glucose, $HCO_3{^-}$, oxygen and ionized $Ca^{2+}$ ($iCa^{2+}$) were significantly decreased, whereas lactate, carbon dioxide, $iMg^{2+}$, ionized $Na^+$ and ionized $K^+$ were significantly increased. During the exhausted swimming, the changes in $iMg^{2+}$ showed a significant negative correlation with changes in pH, glucose, $HCO_3^-$ and $iCa^{2+}$, however a significant negative correlation with changes in lactate and anionic gap. It is concluded that the acute high-intensive exhaustive physical exercise could produced hypermagnesemia, an increase in blood $iMg^{2+}$ via stimulation of $iMg^{2+}$ efflux following increase in intracellular $iMg^{2+}$ from muscle induced by metabolic and respiratory acidosis.

Measurement of the Size Distribution of Smoke Particles with Plastic Types Under Various Fire Conditions (다양한 화재조건에서 플라스틱 종류에 따른 연기입자의 크기분포 변화 측정)

  • Goo, Jaehark;Hwang, Cheol-Hong
    • Fire Science and Engineering
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    • v.31 no.6
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    • pp.8-15
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    • 2017
  • Most fire victims succumb to smoke inhalation, and fire smoke toxicity from interior materials is increasing with increased use of plastics. Large amounts of hazardous effects of smoke are related to deposition of smoke particles in respiratory tracts, and deposition characteristics are influenced by size distribution of particles. Thus, it is essential to know the size distribution of smoke particles from plastics for hazard analysis of fire smoke. In a recent study, it has been shown that size distributions of smoke particles from PP are different from wood in many aspects. In order to know whether other plastics show the same characteristics as PP, size distributions of smoke particles from four plastic materials (LDPE, PA66, PMMA, and PVC) were measured in real time under each fire type with various temperature and oxygen supply. In this study, smoke particles from different plastics were generated uniformly by using steady-state tube furnace method provided in ISO/TS 19700. Their size distributions were measured by using an electrical low pressure impactor (ELPI). Results of measurements showed that size distributions of smoke particles from these four plastic materials were similar to those from PP in many aspects. However, they were distinctively different from those of wood.

Manufacture of Biodegradable Polymer with Wastepaper(I) - Pretreatment and Analysis of Chemical Components On Wastepaper - (폐지를 이용한 생분해성 고분자의 제조(I) - 폐지의 화학적 조성 분석 및 전처리 -)

  • Kwon, Ki-Hun;Lim, Bu-Kug;Yang, Jae-Kyung;Chang, Jun-Pok;Lee, Jong-Yoon
    • Journal of the Korean Wood Science and Technology
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    • v.28 no.3
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    • pp.34-41
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    • 2000
  • Recently many scientists have tried to synthesize biodegradable polymers due to durable and non-biodegradable products of conventional synthetic plastics when these were wasted in nature. So to reuse the wastepapers for biodegradable polymer resources, ONP (old newsprint), OCC (old corrugated containerbpard) were carried out by the pretreatment of chlorinite, hypochlorite and oxygen-alkali treatment conditions. For manufacturing of biodegradable polymer with wastepaper, this study performed to investigate change of chemical components and optimal pretreatment condition. The summarized results in this study were as follows: Lignin content in ONP and OCC was was higher than in MOW and ash content was the highest in MOW. More amount of ash components were reduced by wet defiberation than by dry defiberation. Wet defiberation fiber are better than dry defiberated fiber in chemical pretreatment condition for wastepapers, and the best result was obtained in the condition of sodium chlorite at $70^{\circ}C$, because it has high delignification ratio, ${\alpha}$-cellulose contents and degree of polymerization in this treatment condition. Oxygen-alkali treatment condition is the worst method because of low yield, low degree of polymerization in this pretreatments.

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A New Optical Access Network Structure for the convergence of Broadcast and Communication (방송통신 통합을 위한 새로운 광가입자망 구조에 관한 연구)

  • Hur Jung;Koo Bon-Jeong;Hyun Jae-Myoung;Park Youngil
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2004.11a
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    • pp.17-20
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    • 2004
  • 가정 내에 방송과 총신을 통합하여 제공하기 위한 방법으로서 수동광가입자망(Passive Optical Network)이 제안되고 있다 이를 구현하기 위해 여러 방식들이 연구되고 있는데 크게는 방송과 통신에 각기 다른 파장을 이용하는 방식과, 이들을 시간 다중화 하여 한 개의 과장으로 송신하는 방식으로 나눌 수 있다. 또한 동신에 이용하는 과장의 경우 각 가입자에게 다른 파장을 적용하는 WPON 방식과 모든 가입자에게 한 개의 파장만을 이용하되 시간영역에서 다중화하고 이터넷 프로토콜을 적용하는 EPON 방식으로 구분할 수 있다. WPON의 경우 EPON에 비해 수월하게 광대역 서비스를 할 수 있는 장점이 있으나, 각 가입자 과장의 제어 및 관리가 복잡하며, 고비용을 요구한다. 반면 EPON의 경우 채널의 효율성은 극대화할 수 있으나, 동시 이용자가 많을 경우 전송 속도가 하락한다. 한편, 방송과 통신을 통합한 채널의 특성을 살펴보면 가입자 방향으로의 하향 신호의 양이 중양국 방향으로의 상향 신호의 양에 비해 훨씬 많은 비대칭 구조이다. 따라서 본 연구에서는 이러한 채널 특성에 맞도록 하향 전송에는 WPON의 구조를 이용하고, 상향 전송에는 EPON의 구조를 적용하는 새로운 구조의 광가입자망을 제안하였다. 제안된 구조에 적합한 MPCP (Multi-Point Control Protocol) 프로토콜을 제시하고, 가입자 장치의 여러 종속 신호를 다중화하고 전송할 수 있는 ONU (Optical Network Unit)의 구조를 제안하였다. 또한 이러한 구조를 갖는 W-EPON 테스트베드를 구현하고 전송 시험을 통해 제시된 구조의 적합성을 측정한 결과를 보인다.4 Textual format) 파일을 생성한다. 또한, 콘텐츠 전송 및 저장의 효율성을 위해 이진 포멧인 IPMP화된 MP4 파일을 생성할 수 있다.으로써, 에러 이미지가 가지고 있는 엔트로피에 좀 근접하게 코딩을 할 수 있게 되었다. 이 방법은 실제로 Arithmetic Coder를 이용하는 다른 압축 방법에 그리고 적용할 수 있다. 실험 결과 압축효율은 JPEG-LS보다 약 $5\%$의 압축 성능 개선이 있었으며, CALIC과는 대등한 압축률을 보이며, 부호화/복호화 속도는 CALIC보다 우수한 것으로 나타났다.우 $23.87\%$($18.00\~30.91\%$), 갑폭 $23.99\%$($17.82\~30.48\%$), 체중 $91.51\%$($58.86\~129.14\%$)이였으며 성장율은 사육 온도구간별 차는 없었다.20 km 까지의 지점들(지점 2에서 지점 6)에서 매우 높은 값을 보이며 이는 조석작용으로 해수와 담수가 강제혼합되면서 표층퇴적물이 재부유하기 때문이라고 판단된다. 영양염류는 월별로 다소의 차이는 있으나, 대체적으로 지점 1과 2에서 가장 낮고, 상류로 갈수록 점차 증가하며 지점 7 상류역이 하류역에 비해 높은 농도이다. 월별로는 7월에 규산염, 용존무기태질소 및 암모니아의 농도가 가장 높은 반면에 용존산소포화도는 가장 낮다. 그러나 지점 14 상류역에서는 5월에 측정한 용존무기태질소, 암모니아, 인산염 및 COD 값이 7월보다 다소 높거나 비슷하다. 한편 영양염류와 COD값은 대체적으로 8월에 가장 낮으나

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Air Fuel Ratio Determination Method for Alternative Fuel Based on Carbon Balance and Linear Equation (탄소 균형과 1차식에 의한 대체 연료의 공연비 산정법)

  • Lee, Jae-Won;Kwon, Soon-Tae;Park, Chan-Jun;Ohm, In-Young
    • Journal of Energy Engineering
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    • v.17 no.4
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    • pp.182-188
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    • 2008
  • This paper is to compare the carbon-balanced and liner air-fuel ratio determination methods for alternative fuels. In the previous work, expansion of Eltinge chart, unburned hydrocarbon compensation, comparison of the results from various methods were discussed. It has been also concluded that Eltinge method might be regarded as the most general equation of AFR determination among the existing ones. In the recent years, however, increasing demand for the environmental preservation, including global warming-up protection, and energy conservation lead to introduce the alternative fuel to the internal combustion engine. Therefore, the exact calculations of AFR for these fuels are needed. Especially, for the fuel that contains oxygen, all AFR calculation equations except Eltinge have to be re-formulated. In this paper, the AFR for alternative fuel were calculated by re-formulated carbon balance, accuracy of which was already confirmed, and linear equations, which are newly proposed by statistical method for each fuel. The results show that AFRs based on carbon balance have a little more error compared with gasoline, however, the accuracy is enough for this formula to apply to various fuel. The proposed linear equation also have excellent accuracy below $\lambda=1.2$.

Investigation of Microbiological and Physiochemical Quality for Irrigation Water used in Napa Cabbage Cultivation (배추 재배에 이용되는 농업용수의 미생물 오염도 조사 및 이화학성분 분석)

  • Yun, Bohyun;Kim, Min Kyung;Ryu, Jin Hee;Kim, Won-Il;Park, Byeong-Yong;Kim, Hyun-Ju;Lee, Seung-Don;Kim, Se-Ri
    • Journal of Food Hygiene and Safety
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    • v.32 no.5
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    • pp.396-403
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    • 2017
  • The purpose of this study was to investigate water quality for irrigation water used in Napa cabbage cultivation. The water samples were analyzed for physiochemical and microbiological quality for a total of 111 samples including surface water (n = 75) and groundwater (n = 36) collected from five different regions where Napa cabbage is massively grown. As a conclusion, the levels of fecal indicators for surface water were higher than those for groundwater. The numbers of coliform from surface water and groundwater were 1.96-4.96 and 0-3.98 log MPN/100 mL, respectively. Enterococci were detected in 95% (72/75) of surface water samples and 22% (8/36) of groundwater samples. Besides, 97% (73/75) of surface water samples were observed being contaminated with Escherichia coli, and 22% (8/36) of groundwater sample was positive for E. coli. In the case of surface water, E. coli and coliform correlate to T-P, and enterococci showed relevance to the suspended solid (SS) and biochemical oxygen demand (BOD). In groundwater, fecal indicator bacteria showed relevance to the SS and chemical oxygen demand (COD). These results could be provided as fundamental date for establishing microbial standard of water used in leafy vegetables cultivation.

Effect of Salinity-stratified Waters on Upward Migration and Ratio of Extracted DNA/RNA in Cochlodinium polykrikoides Margalef Based on the Ratio of Absorbance at 260 and 280nm (염분 구배가 Cochlodinium polykrikoides Margalef의 수직이동 및 DNA/RNA 비율에 미치는 영향)

  • Cho Eun Seob;Lee Young Sik
    • Journal of Life Science
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    • v.15 no.3 s.70
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    • pp.468-473
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    • 2005
  • The coastal regions of Yeosu, the South Sea of Korea, has occurred annually the red tide which is caused by potentially ichthyotoxic dinoflagellate C. polykrikoides, with a wide avenue for exchange with oceanic waters and freshwater runoff from Sumjin river. We attempted to examine the variability in response to vertical migration and concentration of extracted DNA/RNA of C. polykrikoides exposed to salinity-stratified waters. The experimental aquarium of the 60 liter was employed to culture C. polykrikoides. One aquarium was supplied with only sea water, the other was consisted of sea water and freshwater. Experiment was conducted for 5 days. In experimental column (mixture of freshwater and sea water), salinity was maintained to 20 at upper and approximately 30 at bottom during the period of this study. The fluctuation with related to dissolved oxygen and pH was similar pattern to both columns. Chlorophyll a was significantly higher value at upper than bottom. During 24h, chlorphyll a on experimental column was extremely high on the top as soon as lighting, compared with control. With elapsed time, the gap between experimental and control columns was a little. In darkness, chlorophyll a was not significantly different between upper and bottom, most cells appeared to randomly distribute on column regardless of water layer. Fluctuation with related to concentration of extracted DNA and RNA based on ratio of absorbance of 260 and 280 nm in experimental column was higher at final day or diel migration than control. These results implied that a large volume of freshwater could be associated with influence of concentration of DNA and RNA, in particular, rapid upward movement caused massive fish kills as soon as sunset.