• The Korean Journal of Pharmacology
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• v.26 no.2
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• pp.161-166
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• 1990

Infusion of bradykinin (BK) into the renal arteries increases sodium excretion. However, it is not clear whether natriuresis results from the renal hemodynamic effects or from the direct effect on renal tubular sodium transport. Therefore, we examined the effects of BK on the transport-dependent oxygen consumption in the distal tubule (DT) and cortical collecting tubule (CCT) of deoxycorticosterone-treated rats. BK inhibited oxygen consumption in a dose-dependent way with a maximal reduction at $0.1\;{\mu}M$ BK. The inhibitory effect of BK was not present in the absence of sodium or in the presence of ouabain (1 mM). These data imply that the inhibitory effect of BK is restricted to the sodium transport-dependent oxygen consumption. We also investigated the relationship between the effect of BK on oxygen consumption and arachidonic acid metabolism. Mepacrine $(10\;{\mu}M)$, an inhibitor of membrane phospholipases, prevented the inhibitory effect of BK, but indomethacin (0.5 mM) didn't. These results suggest that BK decreases the sodium transport-related oxygen consumption in the rat DT and/or CCT, and that it may be mediated by products of enzymes other than cyclooxygenase.

• Journal of Nutrition and Health
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• v.48 no.2
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• pp.180-191
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• 2015

Purpose: There is a lack of data on the energy cost of children's everyday activities, adult values are often used as surrogates. In addition, the influence of body weight on the energy cost of activity when expressed as metabolic equivalents (METs) has not been vigorously explored. Methods: In this study 20 elementary school students 9~12 years of age completed 18 various physical activities while energy expenditure was measured continuously using a portable telemetry gas exchange system ($K_4b^2$, Cosmed, Rome, Italy). Results: The average age was 10.4 years and the average height and weight was 145.1 cm and 43.6 kg, respectively. Oxygen consumption ($VO_2$), energy expenditure and METs at the time of resting of the subjects were 5.41 mL/kg/min, 1.44 kcal/kg/h, and 1.5 METs, respectively. METs values by 18 physical activities were as follows: Homework and reading books (1.6 METs), playing game with a mobile phone or video while sitting (1.6 METs), watching TV while sitting on a comfortable chair (1.7 METs), playing video game or mobile phone game while standing (1.9 METs), sweeping a room with a broom (2.7 METs) and playing a board game (2.8 METs) belong to light intensity physical activities. By contrary, speedy walking and running were 6.6 and 6.7 METs, respectively, which belong to high intensity physical activities over 6.0 METs. When the effect of body weight on physical activity energy expenditure was determined, $R^2$ values increased with 0.116 (playing a game at sitting), 0.176 (climbing up and down stairs), 0.246 (slow walking), and 0.455 (running), which showed that higher activity intensity increased explanation power of body weight on METs value. Conclusion: This study is important for direct evaluation of energy expenditure by physical activities of children, and it could be used directly for revising and complementing the existing activity classification table to fit for children.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.1
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• pp.52-58
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• 1991

Mass mortality of Halocynthia roretzi has been occurred in culturing grounds of southern coast of Korea during the long period of summer drought and the unusal high water temperature in 1988. Especially Halocynthia roretzi was damaged severely in 2 years group. Therefore we verified these phenomena by observing the environmental factors of culturing grounds and by evaluating oxygen consumption rate in indoor experiment. In culturing grounds, the mortality rate was high in Namhae Mizo area where water temperature was the highest and the lowest in dissolved oxygen concentration as low as 3.76m/l in field observation. In indoor experiment, the higher the water temperature, the more mortality was occurred and the salinity above 30 had no effect on its mortality. In oxygen consumption rate experiment, the smaller needs more oxygen than larger one, i. e., oxygen demand per unit weight was higher in 2 years group than that of 3 years. The theoretical and the actual minimum oxygen demands(MOB) for preventing asphixation was 3.7ml/kg/h at $20^{\circ}C$ and 6.3ml/kg/h gat $25^{\circ}C$, But actual MOD was 4.3ml/kg/h at $25^{\circ}C$ in indoor experiment. It seemed that mass mortality of Halocynthia roretzi in summer was caused by metabolism disorder due to insufficient oxygen level influenced by the high water temperature and the high density of Halocynthia roretzi, which results in the paucity of oxygen especially in 2 years groups.

• Korean Journal of Environmental Biology
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• v.27 no.3
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• pp.285-291
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• 2009

The effects of water temperature on oxygen consumption (OC) of starry flounder Platichthys stellatus reared in seawater (SW) and freshwater (FW) was performed in closed water-recirculating system containing respiratory chamber. Fish acclimated in separate indoor tanks with SW (nine of fish used, $263.0{\pm}40.4$ g) or FW (nine of fish used, $265.8{\pm}34.8$ g) were sampled. The OC of starry flounder at $15^{\circ}C,\;20^{\circ}C$ and $25^{\circ}C$ were $74.4{\pm}17.0,\;85.9{\pm}15.8,\;98.3{\pm}11.4\;mg\;O_2\;kg^{-1}hr^{-1}$ in SW and $46.7{\pm}12.0,\;63.3{\pm}7.5,\;82.6{\pm}5.3\;mg\;O_2\;kg^{-1}hr^{-1}$ in FW, respectively, showing a linear increase in OC with water temperature. The OC of fish reared in both SW and FW clear diel rhythm, with lower values at daytime and higher values in the night, in accordance with light (09:00~21:00 hr) and dark (21:00~09:00 hr) phases of the diel cycle (12L : 12D) in water temperature at $15^{\circ}C$ and $20^{\circ}C$. However OC of fish reared in both SW and FW showed unclear diel rhythm with light and dark phases of the diel cycle in water temperature at $25^{\circ}C$. Starry flounder reared in FW had higher ventilation rates than those in SW, but SW had higher OC per breath than those in FW.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.3
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• pp.496-504
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• 1997

This study was carried out to estimate toxic effects of phenol on survival and metabolism of the abalone juvenile, Haliotis discus hannai. The experiment was conducted by renewal bioassay procedure with different salinities at $20^{\circ}C$. The $LC_{50}$ of the juvenile exposed to phenol in the range of 0.5 and $100mg/\ell\;was\;34.3\~6.5mg/\ell\;at\;2.4\%_{\circ}\;and\;52.2\~9.3m/\ell\;at\;32\%_{\circ}$ salinity with exposure time from 24 hours to 96 hours. $LT_{50}$ was remarkablely reduced with increase of phenol conentration and decrease of salinity. Lethal toxicity or phenol was higher at low salinity than at high salinity. Therefore, salinity is likely to be one of factor to increase phenol toxicity. The oxygen consumption of the juvenile was reduced with increase of phenol concentration and with decrease of salinity. In spite of phenol toxicity, the oxygen consumption of the juvenile exposed to phenol of low concentration was high and similar as compared with that of control group. Survival rates of the abalone kept in phenol-free sea water after exposure to phenol concentration of 5, 10 and $20mg/\ell$ for 96 hours were reduced with decrease of salinity. Durations required to recover the normal metabolic rate of the juvenile, which was exposed to phenol concentration of 5, 10 and $20mg/\ell$ for 96 hours, were made longer with increasing phenol concentration. In the case of the juvenile exposed to sublethal concentration of phenol for 15 days, it were elongated as compared with that of the abalone exposed to phenol concentration caused acute toxicity. The result of this experiment indicated that relatively low concentration of phenol can impact on the abalone juvenile in marine ecosystem.

• Journal of fish pathology
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• v.25 no.1
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• pp.47-58
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• 2012

Behavioral and physiological responses of juvenile red seabream (Pagrus major) to different concentrations of ethanol were investigated. No swimming and no reaction to touching by a wooden stick was observed at 0.6% ethanol group in behavioral response, and survival rate was 100% after 5 hours of treatment. Red blood cell count, hematocrit and hemoglobin levels in plasma were not significantly different among all groups. AST activities in plasma significantly decreased as ethanol concentration increased. On the contrary, ALT activities in plasma significantly increased as ethanol concentration increased. Cortisol level in plasma was the lowest in 0.6% ethanol group. Glucose levels in plasma increased significantly when ethanol concentration increased more than 0.4%. Oxygen consumption of fish in 0.6% ethanol seawater was constantly lower than that of fish in control seawater from 2 hours after the exposure to ethanol seawater until the end of experiment.

• Journal of Aquaculture
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• v.18 no.1
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• pp.45-51
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• 2005

The optimum salinity and the effects of rapid salinity change on oxygen consumption and ammonia nitrogen excretion were examined in River Puffer Takifugu obscrus (total length 9.5$\pm$0.9 cm, total weight 18.7$\pm$5.4 g). Fish examined at the different transfer medium salinity (2, 12, 22 and 32 psu) after 2 months of acclimation period at each salinities. The routine metabolic rates of River puffer are shown as parabola equation, $Y=-0.0873X^2+0.6384X-0.690$ for oxygen consumption and $Y=-2.1667X^2+7.1672X+31.999$ for ammonia nitrogen excretion with the salinity medium at 2, 12. 22 and 32 psu. The oxygen consumption and ammonia nitrogen excretion of River puffer trans-ferred to the low salinity medium (2 and 12 psu) showed significantly difference in each salinities rearing groups than to salinity of 22 and 32 psu. Fish has a diurnal rhythm in relate to feeding, it was showed that the peak of oxygen consumption appeared at 3 hours after feeding and the ammonia nitrogen excretion rate reached maximum 4 hours after feeding. These results may indicate that the optimum salinity for rearing of River puffer is 22 psu based on growth and feed conversion ratio. The rapid change of medium salinity had no effects on the oxygen consumption and nitrogen excretion in River puffer based on this experiment.

• The Korean Journal of Zoology
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• v.4 no.2
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• pp.20-24
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• 1961

The present experiment was conducted to investigate the rate of oxygen consumption in relation to the several amino acids in the Mang-Kong frog ,Gallula tornieri VOGT. 1. When the liver tissue was suspended in the Ringer's solution (0.85% NaCl), the amount of oxygen consumption per hour was 0.043$\mu$l/mg , whereas when it was suspended in M/15, pH 5.8 phosphate buffer the oxygen consumption reached 0.93$\mu$l/mg. 2. When 0.5ml of 1% alanine and 0.5ml of 0.5% arginine were used as respiratory material the amount of oxygen consumption increased remarkably, while on the other hand glycine showed little influence on the amount of oxygen consumption. 3. When 0.5ml of 0.1% , 0.5% alanine-arginine mixture, 0.5ml of 0.1% , 0.5% alanine-glycine mixture and 0.5ml of 1 % arginine-glycine were added the oxygen consumption in the liver tissue increased somewhat in comparison to the case in which single amino acid was used. 4. Glycine appeared to have influence on the respiration of the liver tissue but the amount of oxygen consumption increased as the concentration of arginine-glycine mixture increased, whereas the rate of oxygen consumption increased remarkably as alaninie-glycine mixture decreased. It was evidenced that in the combination of alanine-arginine the rate of oxygen consumption increased as the concentration of the mixture decreased and vice versa.

• Journal of Preventive Medicine and Public Health
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• v.10 no.1
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• pp.134-137
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• 1977

The most efficient method for reducing the organic content of dilute liquid waste is by aerobic-biological treatment. Basically, the organisms responsible for treatment possess the ability to decompose complex organic compounds and to use the energy so liberated for their bodily functions: reproduction, growth, locomotion and so on. That part of organic matter used to produce energy is converted to the essentially stable end products of carbon dioxide, water and ammonia, while the remainder is converted to new cells which can be settled and thus removed from the liquid before the waste is discharged to the receiving body water. Oxygen must be supplied continuously during the aerobic process. In the field of sewage treatment the Warburg respirometer is used mainly for the measurement of the oxygen uptake of samples. In this experiment the Warburg constant volume respirometer was used to determine the oxygen uptake by bacteria in the presence of various glucose concentrations. The rate of oxygen uptake by the bacteria was expressed as the respiratory quotient. The result indicated that the oxygen uptake was proportional to the glucose concentration. The expecting equation of the regression line was Y=7.7+0.12X where Y: respiratory quotient, ${\mu}l.\;O_2$ taken up/mg. dry wt. bacterium/hr. X: concentration of glucose, mg/l

• The Korean Journal of Malacology
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• v.9 no.2
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• pp.23-29
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• 1993

전복, Nordotis discus의 1$0^{\circ}C$와 2$0^{\circ}C$의 수온구에서 개체크기별 대사와 폐사에 미치는 부니의 영향에 대한 결과는 다음과 같다.1. 수온 -염분별 산소소비율의 일반적 경향은 고온-고염분에서 큰개체보다는 작은개체에서 그 비율리 높게 나타났다.2. 염분별 두 수온간의 온도계수 (Q $_{10)}$ 는 작은 개체에서 1.2-2.9의 병위를 큰개체의 경우 1.5-3.4의 범위를 나타내었으나 개체크기에 관계없이 유사한 양상을 보였다.3. 부니의 농도별 사망율은 작은 개체의 경우 처리 96시간후 2$0^{\circ}C$수온에서 부니 농도1000ppm에서 가장 높았고. 큰 개체의 경우 50ppm에서 가장 낮았다.4. 96시간-반치사농도(LC$_{50}$)는 1$0^{\circ}C$의 경우, 큰개체에서 1076.58 ppm, 작은 개체에서 812.55 ppm이었으며, 2$0^{\circ}C$의 경우는 큰개체에서 698.28 ppm, 작은 개체에서 546.74ppm으로 나타났다.5. 부니의 영향으로 인한 호흡산소소비량은 개체크기에 관계없이 1$0^{\circ}C$에서 보다 2$0^{\circ}C$에서 더욱 높았으며, 1000ppm에서 70% 이상의 감소율을 나타내었다.