• Journal of Ginseng Research
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• v.26 no.4
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• pp.202-205
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• 2002

This study was carried out to investigate the optimum conditions for extraction of acidic polysaccharide from red ginseng marc produced by manufacturing alcoholic and water extract from red ginseng. Extraction efficacy of acidic polysaccharide from dried red ginseng marc was higher than that before drying. The appropriate conditions for the extraction of acidic polysaccharide from red ginseng marc were particle size under 3.35 mm after drying red ginseng marc, 1∼2 hours of extraction time and 2∼3 extraction times, respectively. The amount of acidic polysaccharide in water extract from red ginseng marc treated with $\alpha$-amylase and cellulase increased about 20∼50%. From the above resuts, we suggest that red ginseng marc produced by manufacturing alcoholic and water extract of red ginseng has higher potencies in the utilization of waste materials.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.4
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• pp.736-740
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• 2004

Preparation of ginseng concentrate with high content of acidic polysaccharide from white tail ginseng marc that was obtained after preparation of white tail ginseng extract. As a result of extraction of white tail ginseng under various concentrations of ethanol (0∼90%), both amount of acidic polysaccharide and extraction yield decreased by increasing the ethanol concentration. However, acidic polysaccharide extracted by water from white tail ginseng marc was increased in accordance with the increase of ethanol concentration. The optimal condition for the extraction of acidic polysaccharide from the marc was treatment of $\alpha$-amylase in 390∼650 unit/g residue/15 mL of distilled water for 5 min at 4$0^{\circ}C$. The amount of acidic polysaccharide in water extract of the marc was increased from 8.3% to 10.5% by the treatment of $\alpha$-amylase. A new ginseng extract mixture was manufactured by mixing 50% ethanol extract of white tail ginseng and water extract of alcoholic residue in the ratio of 8:2 (w/w). Crude saponin content and acidic polysaccharide content were 10.5% and 17%, respectively. The mixture had a same crude saponin content and twice acidic polysaccharide content comparing to 50% ethanol extract of white tail ginseng. It suggests that preparation of new ginseng concentrate with high content of acidic polysaccharide from white tail ginseng marc has high potencies in the utilization of waste material.

• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.915-923
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• 2017

The by-products of red ginseng marc showing the improvement of health has been strongly studied because of the expectation of possibility to be used as the functional foods. This research was to investigate the extraction, separation, isolation, and evaluation of crude acidic polysaccharides related to antioxidant activity, sequently chemical structure of those products was evaluated by FT-IR and NMR. Compared with other solvents such as ethanol, methanol, propanol, acetone, and butanol, ethanol was selected. The concentration of red ginseng solution for extraction was selected as 10%(w/v) related to extraction yield(11.95%) and amount(11.8 mg/ml). In the ion exchange chromatography, aidic polysaccharides showing the highest antioxidant activity was obtained when eluted by distilled water. As results of structural analysis by FT-IR and NMR, peaks corresponding to C-O, C-O-O-, C-H, anomeric C-6, and repeated C-1 and C-6 linkages was to be presumed to be ($1{\rightarrow}6$) glycosidic linkage with the typical acidic polysaccharide.

• Food Science and Preservation
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• v.10 no.2
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• pp.206-212
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• 2003

Comparative effects of electron beam and gamma-ray irradiation (25 ∼15 kGy) were investigated on saponin stability and some physiological and chemical properties of white and red ginseng powders. Saponin components were found stable upon irradiation of both energies when determined by TLC and HPLC, after 4 months of storage at room temperature as well as immediately after treatment The contents of total phenolics and acidic polysaccharides of the samples were higher in red ginseng than in white. Polysaccharide contents increased with irradiation doses. Amylase activity of white ginseng was stimulated by irradiation but decreased with the lapse of storage time. There were no apparent differences in electrophoresis patterns of extracted proteins depending on irradiation doses and energy sources.

• 인삼약초
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• v.4 no.1 s.11
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• pp.77-82
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• 2006

• Proceedings of the Ginseng society Conference
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• 1998.05a
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• pp.42-42
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• 1998

• Proceedings of the Ginseng society Conference
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• 2005.11a
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• pp.101-116
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• 2005

• Food Science and Preservation
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• v.30 no.5
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• pp.857-867
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• 2023

In this study, the extraction yield, acidic polysaccharides and ginsenosides of red and black ginseng were optimized by using the response surface methodology in consideration of the ethanol concentration and temperature of the extraction. The R² of the model formula for the yield, acidic polysaccharides and ginsenosides was 0.8378-0.9679 (p<0.1). An optimal extraction yield of 5.29% was reached for red ginseng soluble solids when 1.52% ethanol concentration was used at a temperature of 67.27℃. Additionally, the optimal extraction yield for black ginseng soluble solid was 6.11% when 3.12% ethanol concentration was used at a temperature of 66.13℃. Furthermore, the optimal conditions for extracting acidic polysaccharides from red ginseng were using an ethanol concentration of 4.03% at a temperature of 69.61℃; a yield of 1.86 mg/mL was obtained. The optimal extraction yield for acidic polysaccharides from black ginseng was 1.80 mg/mL when extracted using a concentration of 24.67% of ethanol at a temperature of 71.14℃. An optimal extraction yield of 0.22 mg/mL was reached for ginsenoside Rg₁ from red ginseng when 79.92% ethanol concentration was used at a temperature of 70.62℃. The optimal extraction yield of ginsenoside Rg₃ from black ginseng was 0.31 mg/mL when ethanol was used at a concentration of 75.70% at a temperature of 65.49℃. The ideal extraction conditions for obtaining the maximum yield of both acidic polysaccharide and ginsenoside from red and black ginseng were using ethanol at a concentration between 35 and 50% at an extraction temperature of 70℃.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.11
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• pp.1665-1673
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• 2014

The objective of this study was to compare the compositions and immuno-enhancing effects of 6-year-old red ginseng powder (RGP) with those of its fractions. RGP was subjected to extraction with 100% ethanol to obtain an ethanol fraction (E) and residue 1 (R1). Then, R1 was subjected to extraction with distilled water to obtain water fraction (W) and residue 2 (R2). Chemical compositions were as follows: 4.94% acidic polysaccharides and 1.56% ginsenosides (amounts of Rg1, Re, Rf, Rg2, Rb1, Rc, Rd, and Rg3) in RGP, 0.11% acidic polysaccharides and 6.99% ginsenosides in E, 4.93% acidic polysaccharides and 0.40% ginsenosides in R1, 0.50% acidic polysaccharides and 0.30% ginsenosides in R2, and 7.46% acidic polysaccharides and 0.61% ginsenosides in W. Immuno-enhancing effects of fractions from RGP were examined based on suppression of immune responses by cyclophosphamide. In the first fraction test, the antibody response to SRBCs increased significantly in the R1-treated group, but not the E-treated group. In the second fraction test, W showed higher immuno-enhancing effect than R1 and R2. W, which contained the highest amount of acidic polysaccharides, restored numbers of T and B cells, macrophages, as well as $CD4^+$ and $CD8^+$ T cells in the spleen suppressed by cyclophosphamide. These results suggest that acidic polysaccharides from red ginseng may be more effective than saponin in enhancing immune functions and reducing immunotoxicity of cyclophosphamide.

• The Korean Ginseng Research and Industry
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• v.6 no.2
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• pp.2-21
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• 2012

현대과학에 의하여 밝혀진 고려인삼의 약리효능을 보면 뇌 기능 개선, 통증감소, 암 예방 및 항암작용, 면역기능 항진, 간 기능 개선, 항당뇨, 혈압 조절기능, 항피로, 항 스트레스, 여성 갱년기장애 개선, 남성 발기부전 개선, 항 산화, 항 노화 등에 관한 효능을 들 수가 있으며, 고려인삼의 효능성분으로서는 ginsenosides(인삼사포닌), 페놀화합물, 폴리아세틸렌화합물, 다당체 특히 산성다당체, 펩타이드 등이 주류를 이루고 있다. 효능성분 중에는 특히 ginsenosides에 관한 연구가 많이 수행되었는 바, 고려인삼이 타국삼에 비하여 다양한 종류의 ginsenosides가 함유되어 있다는 것이 판명되었다.