• Title/Summary/Keyword: 산란초기

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The Effect of Organic solvents on Interrupting Diapause Initiation in Prospective Diapause Eggs of Bombyx mori. (가잠란의 휴면개시조지에 관한 유기용매의 효과)

  • 김삼은
    • Journal of Sericultural and Entomological Science
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    • v.27 no.1
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    • pp.24-30
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    • 1985
  • The effect of some organic solvents on interrupting diapause of Bombyx mori eggs was examined to provide a clue to the mechanism of diapause initiation. Methyl alcohol and benzene proved to be efficient in developing the prospective diapause eggs upto the stage of hatching or body pigmentation of the embryos. On the other hand, most of the eggs soaked in chloroform and mixture solution of chloroform-methyl alcohol (2:1) died in the early embryonic developmental stage with yellow or red brown colours, and the egg weights decreased upto ca. 40% of the original weight 8 days after the treatments. Methyl alcohol treatment for 2, 5 and 10 min to the 5hr-old-eggs led to empergence of the larvae, with high incidence (70∼80%) in the race of Kumchu X Chonghwa and with low indidence (1∼4%) in Daezo. The effect of same treatment to 20hr old eggs decreased to ca. 10% in the emergence of larvae in Kumchn X Chonghwa and increased to 20∼30% in Daezo, while the effect disappeared shortly after the diapause initiation (48hr-old-eggs). Considering the high dependency upon the egg age of the sensitivity to solvents, it was supposed that initiation and termination of diapause may be controlled by different mechanism. It was also suspected that the solvents exert their effect on the permeability of the eggshell.

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Turbid water atmospheric correction for GOCI: Modification of MUMM algorithm (GOCI영상의 탁한 해역 대기보정: MUMM 알고리즘 개선)

  • Lee, Boram;Ahn, Jae Hyun;Park, Young-Je;Kim, Sang-Wan
    • Korean Journal of Remote Sensing
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    • v.29 no.2
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    • pp.173-182
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    • 2013
  • The early Sea-viewing Wide Field-of-view Sensor(SeaWiFS) atmospheric correction algorithm which is the basis of the atmospheric correction algorithm for Geostationary Ocean Color Imager(GOCI) assumes that water-leaving radiances is negligible at near-infrared(NIR) wavelengths. For this reason, all of the satellite measured radiances at the NIR wavelengths are assigned to aerosol radiances. However that assumption would cause underestimation of water-leaving radiances if it were applied to turbid Case-2 waters. To overcome this problem, Management Unit of the North Sea Mathematical Models(MUMM) atmospheric correction algorithm has been developed for turbid waters. This MUMM algorithm introduces new parameter ${\alpha}$, representing the ratio of water-leaving reflectance at the NIR wavelengths. ${\alpha}$ is calculated by statistical method and is assumed to be constant throughout the study area. Using this algorithm, we can obtain comparatively accurate water-leaving radiances in the moderately turbid waters where the NIR water-leaving reflectance is less than approximately 0.01. However, this algorithm still underestimates the water-leaving radiances at the extremely turbid water since the ratio of water-leaving radiance at two NIR wavelengths, ${\alpha}$ is changed with concentration of suspended particles. In this study, we modified the MUMM algorithm to calculate appropriate value for ${\alpha}$ using an iterative technique. As a result, the accuracy of water-leaving reflectance has been significantly improved. Specifically, the results show that the Root Mean Square Error(RMSE) of the modified MUMM algorithm was 0.002 while that of the MUMM algorithm was 0.0048.

1세포기 닭 수정란의 체외배양과 대리난각 배양에 있어서 수정란의 조건과 대리난각의 조건이 부화율에 미치는 영향

  • 이지현;김이헌;강영란;신귀인;박해진;오세태;유이식;장원경;김창근
    • Proceedings of the KSAR Conference
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    • 2003.06a
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    • pp.70-70
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    • 2003
  • 조류의 배자는 포유류의 배자처럼 모체로부터 영양분을 공급받는 것이 아니라 알속에 저장되어 있는 영양물질로 발육한다. 배자의 발육은 대부분 체외에서 진행된다. 난자는 배란된 후 수정되어 난관팽대부에서 1세포기 수정란이 된다. 그 후 난관협부로 이동하여 최초로 분열이 일어나 배자의 발육이 진행되고, 산란시에는 세포수가 약6만여 개에 달한다. 따라서 수정란에 유전조작기법을 사용하기 위해서는 모체의 난관속에서 일어나는 배발생과 난각속에서 일어나는 개체발생을 위한 체외배양법과 대리난각 배양법이 확립되어 있어야 한다. 그와 같은 기술은 닭 수정란의 배 발생 관찰 및 분석과 유용한 물질을 생산하기 위한 형질전환 가금 연구에 중요한 기술로 사용되고 있다. 따라서 본 연구는 1세포기 닭 수정란의 체외배양과 대리난각 배양에 있어서 수정란의 조건과 대리난각의 조건이 배자의 생존율과 부화율에 미치는 영향을 조사하여 체외배양과 대리난각 배양에 의한 병아리 생산 효율을 제고하기 위한 기초자료를 얻고자 실시하였다. 주요 조사 항목은 수정란의 채란위치, 배자의 발생단계, 수정란의 무게, 대리난각용 계란의 보존 기간, 대리난각의 두께, 대리난각 두께의 감소율, 대리난각의 크기 등을 조사하여 배자의 생존율과 부화율에 미치는 영향에 대하여 조사하였다. 본 실험의 결과는 초기 발생이 빠른 배자가 생존율과 부화율이 높았으며, 본 실험에 사용한 대리난각용 계란의 보관기간이 짧을수록 배자의 생존율과 부화율이 높은 것으로 조사되었다(p<0.05). 그러나 대리난각용 계란의 크기와 대리난각의 두께 차이는 배자의 생존율과 부화율에 큰 영향이 없는 것으로 조사되었다.하였을 때 분할율은 68.0%였으며, 이중 12.0%가 상실배 또는 배반포로 발달하였다. 뿐만 아니라 10% FBS가 첨가된 TCM-199 배양액에 난관상피세포와 공배양을 실시하였을 경우는 72.0%가 분할하였으며, 이중 16.7%가 상실배 또는 배반포로 발달하였다. 이상의 결과로 볼 때 활성화 처리는 ionomycin과 6-DMAP 용액처리가 적합하며, 단위발생란의 체외배양은 보다 적합한 배양조건의 확립이 필요한 것으로 생각된다.icalcium lactate 공동배양은 체외배양에 별다른 영향을 미치지 못하는 것으로 나타났다.생산하는 것을 확인할 수 있었다.4시간에 등급별 회수율이 각각 GI(27.4%), GII(14.7%), GIII(43.2%), GIV(14.7%)로 나타났으며, 46~50시간에는 GI(12.0%), GII(12.0%), GIII(28.0%), GIV(48.0%)로 나타났다. 이상의 결과로 볼 때 미성숙 난자의 회수는 hCG 투여 후 29~34시간이 적합한 것으로 생각된다. 가금의 생산에 있어서 매우 효율적이고 주목할 만한 방법으로 사료된다. 것으로 나타났다.적외선.열풍 복합건조방법이 높게 나타나 이것은 곡물 표면에 원적외선 방사에의한 복사열이 전달되어 열장해를 받았기 때문으로 판단되며, 금후 더 연구하여 적정 열풍온도 및 방사체 크기를 구명해야 할 것이다.으로 보여진다 따라서 옻나무 유래 F는 포유동물의 생식기능에 중요하게 작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도

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REPRODUCTIVE CYCLE AND BREEDING SEASON OF THE FRESHWATER CLAM, ANDONTA (SINANODONTA) WOODIANA(LEA) (담수산 진주패 뻘조개, Anodonta(Sinanodonta) woodiana(Lea)의 생식주기 및 임란기)

  • 정의영
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.13 no.4
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    • pp.135-144
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    • 1980
  • The reproductive cycle and the breeding season of the freshwater clam, Anodonta (Sinanodonta) woodiana (Lea) have been investigated by histological examination of the gonadal development under photomicroscopy. The materials were monthly collected from the Nakdong River for one year from September 1979 to August 1980. Sexuality of Anodonta (Sinanodonta) woodiana is dioecious, and the species are ovoviviparous. The gonads are irregularly arranged from the subregion of mid-intestinal gland in visceral cavity to reticular connective tissue of foot. The ovary is composed of a number of small ovarian sacs, The epithelium of ovarian sac has a function of the germinal epithelium. Oogonia actively proliferate along the germinal epithelium of the ovarian sac, in which young oocytes are growing. The testis is composed of a number of seminiferous tubules, and the epithelium of the tubule has function of germinal epithelium, along which spermatogonia actively proliferate. A great number of undifferentiated mesenchymal tissue and eosinophilic granular cells are abundantly distributed between the growing oocytes and spermatocytes in the early development stages. With the further development of the ovary and testis these tissues and cells gradually disappear. Then the undifferentiated mesenchymal tissue and granular cells are considered to be related to the growing of the oocytes and spermatocytes. The gonads had function year-round the individuals which have various developmental stages of gonads appearing all the time. Spawning continued year-round except for the period of high temperature of water, during August and September. The peak spawning seasons appeared twice a year between January and March, and between June and July in 1980. Individuals which have trochophore larvae in the marsupium of the adult appeared year-round except September 1579 and August 1980. The rate of individuals which have glochidia in the marsupium was 72.7 percent in May 1980 which was the highest brooding fate.

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Studios on the Pen Shell Culture Development (I) -Reproductive Ecology of Pen Shell in Yoja Bay- (키조개의 양식개발에 관한 연구(I) -여계만산 키조개의 번식생태-)

  • Yoo Sung Kyoo;Yoo Myong-Suk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.6
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    • pp.529-535
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    • 1984
  • This study aims at obtaining the basic data for the development of pen shell culture at Yoja Bay, the southwest coast of Korea. The authors dealt with the annual gonadal changes of the pen shell, Atrina pectinata japonica, to find out the gametogenesis, reproductive cycle and spawning season, and to investigate the fluctuation in the larval occurence in Yoja Bay. From July 1983 to June 1984, an average of 20 individuals as specimens were monthly collected by commercial fishing dredge near Manwol island located at the mouth of Yoja Bay. The degree of gonadal development was determined by the histological observations as resting spent stage, early developmental stage, after developmental stage, early spawning stage, after spawning stage and degenerative stage. According to these degrees of gonadal development, annual reproductive cycle of the pen shell population was determined. From July to August, the gonads were changed through degenerative into resting stage and, in September and October, they became entirely empty gonads. From November they showed the first sign of gametogenesis developing very slowly. Nevertheless, the developing gametes did not increase in number probably owing to a phagocytic phenomenon by phagocytes which appeared in the gonad during this stage. Some individuals started spawning in April and in May majarity of individuals were in spawning stage. In June, majority of the individuals showed signs of degenerative stage. Therefore, the authors came to a conclusion that the pen shells in Yoja Bay spawn chiefly in May. And this is also supported by the result of the survey on the planktonic occurrences of the pen shell larvae. Namely, there were no larvae at all in April, only a few in May and many in June by vertical water sampling.

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Spawning Behavior and Early Life History of Endangered Cottus hangiongensis (멸종위기에 처한 한둑중개(Cottus hangiongensis)의 산란습성 및 초기생활사)

  • Seo, Won-Il;Yoo, Dong-Jae;Byun, Soon-Gyu;Kim, Yi-Cheong;Lee, Sung-Hun;Yeon, In-Ho;Han, Kyeong-Ho;Yim, Hu-Soon;Lee, Bae-Ik
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.1
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    • pp.46-53
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    • 2010
  • Spawning behavior and early life history of the tuman river sculpin, Cottus hangiongensis were studied in the laboratory and in the field at Wangpi Stream, Gyeongsangbuk-do, Korea, from January to December, 2007. The spawning ground was in the lower Wangpi Stream, which is a shallow region about 40cm or less in depth. During the spawning period, from March to April, mature males made nest cavities under stone 10 which they led a gravid female. The male and female then turned upside down, and spawning and fertilization occurred onto the ceiling of the nest cavity. After spawning, the male chased the female from the nest and mated with several other females. Fertilized eggs were spherical in shape, demersal, adhesive, transparent and yellow in color, measuring 1.86 mm (1.79~1.93 mm) in diameter. A mean of 17(12~22) various-sized oil globules were counted in the yolk. Granular materials formed a mass in the yolk. Fertilized eggs hatched at 256 hrs, 10 minutes after the morula stage under water temperature of $15.0{\sim}18.0^{\circ}C$. Newly hatched larvae 9.34 mm (9.02~9.69 mm. n=10) in total length (TL) had a large yolk At 14 days after hatching, larvae 11.40 mm (11.07~11.72 mm, n=10) in TL transformed to the postlarval stage. At 41 days after hatching, postlarvae of 18.42 mm (17.31~18.62 mm, n=10) in TL had reached the juvenile stage. The result of this study indicate that Cottus hangiongensis has the spawning ground in the lower stream and the amphidromous life history which is the different from that of Cottus poecilopus.

Mineralogical Characteristics and Genesis of Phlogopite in the Talc Deposits of the Chungnam Area, Korea (충남지역 활석광상에서 산출되는 금운모의 광물특성 및 기원)

  • Kim Geon-Young;Kim Soo Jin;Koh Yong Kwon;Bae Dae Seok
    • Journal of the Mineralogical Society of Korea
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    • v.17 no.3
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    • pp.221-233
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    • 2004
  • Mineralogical characteristics and genesis of phlogopite in the talc deposits of the chungnam area were studied. Mica is one of the major impurity of talc ores in the study area. Talc-related micas show typical phlogopite composition, whereas talc-unrelated micas show wide compositional variations between biotite and phlogopite. Phlogopite mainly occurs in the black-wall type zone, especially in the nodular talc ores near the outer part of talc ore bodies. Interleaving textures of phlogopite and chlorite are easily observed under the optical microscope and back-scattered electron images. Interleaving textures of phlogopite and talc are observed also. Examination of the phlogopite by transmission electron microscope reveals that 14 $\AA$ layers of chlorite are randomly interlayered within the 10 $\AA$ layers of phlogopite, which suggests that the genesis of phlogopite is closely related to chlorite. Considering the occurrence and mineralogical characteristics of phlogopite, and the possible origin of K for the formation of phlogopite, phlogopite of the study area was formed by interaction between talc ore body and hydrothermal solution containing sufficient K at the late stage of talc formation. K might be introduced from the granitic gneiss at the contact zone between the talc ore body and the granitic gneiss under favorable structural condition for the potash metasomatism.

Properties of Au Clusters Supported on $TiO_2$ Studied by XPS, ISS, AES, and TPD (XPS, ISS, AES, TPD를 이용한 $TiO_2$ 위에 지지된 Au 클러스터의 특성 연구)

  • Kim, Dae Young
    • Journal of the Korean Chemical Society
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    • v.42 no.6
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    • pp.607-617
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    • 1998
  • Au was dosed on $TiO_2(001)$ film grown epitaxially on Mo(100) surface in about 90 ${\AA}$ thickness. The growth mode of Au, thermal behavior and stability of the Au clusters, and the binding energy shift of Au 4f with the change in the amount of Au loading were studied by Auger Electron Spectroscopy (AES), Temperature Programmed Desorption (TPD) spectroscopy, Ion Scattering Spectroscopy (ISS), and X-ray Photoelectron Spectroscopy (XPS). Au grows three dimensionally on $TiO_2(001)$ film and the average size of Au clusters prepared at low temperature is smaller than those at higher temperature and the size increases with temperature irreversibly. Au clusters on $TiO_2(001)/Mo(100)$ start evaporation at 1000 K. TPD spectra of Au show very asymmetric peaks with the same leading edges irrespective of the amount of Au loading. The temperature at the peak maximum increases with the amount of Au. The desorption energy of Au obtained from the leading edge analysis of the TPD spectra is about 50 kcal/mol. The initial sticking coefficient of Au on $TiO_2(001)$ is constant in the temperature range of 200-600 K. The binding energy of Au 4f for the Au loaded on the film less than 2.0 MLE shifts to higher energy compared with the bulk Au. The shift is +0.3 eV at 0.1 MLE Au amount.

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Studies on Life Cycle of Rice Leaf Beetle (Oulema oryzae KUWAYAMA) and Control Method (벼잎벌레 생태(生態)와 피해해석(被害解析) 및 방제(防除)에 관(關)한 연구(硏究))

  • Yu, B.J.;Kim, C.O.;Chung, S.M.;Park, Y.S.;Huh, B.L.
    • Korean journal of applied entomology
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    • v.24 no.1 s.62
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    • pp.25-28
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    • 1985
  • Experiments were carried out to investigate the life cycle of the rice leaf beetle (RLB), (Oulema oryzae KUWAYAMA) ,yield losses by its damages, and control method in $1976{\sim}1983$ at the experimental field of Gangweon PORD. Peak occurrence of adult and larva rice beetle was late May and mid-June, respectively. Adult overwintered in the forest of mountain and move into the paddy field to lay eggs on the rice leaf from late seedling stage. A female adult laid eggs for 2 days, and egg, larval and pupal period was 9, 16, and 9 days, respectively. Leersia japonica MAKINO was damaged slightly by the beetle. The yield reduction by artifical defoliation ranged from 6 to 40% and yield reduction increased as leaf feeding delayed until mid-July. All insecicides show 100% of control value against RLB.

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Reproductive Cycles of Moroco oxycephalus and M. lagowskii in Korea (한국산 버들치와 버들개의 생식 주기에 관한 연구)

  • Kang, Young-Jin;Min, Mi-Sook
    • Korean Journal of Ichthyology
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    • v.11 no.2
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    • pp.117-125
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    • 1999
  • We investigated the reproductive cycles of two freshwater fishes, Moroco oxycephalus and M. lagowskii, in Korea. Seasonal changes in gonadosomatic index (GSI) and gonads were investigated histologically from April 1998 to April 1999. The reproductive cycles of two species were not shown any differences. The reproductive cycle can be divided into 5 phases : phase I (spent phase), phase II (immature phase), phase III (early developing phase), phase IV (late developing phase), and phase V (ripe phase). In phase I, the gonads of two species began to lose distinctly their weights from mid April, and reached the lowest GSI in late July (phase II). In September, the GSI values of testis and ovary increased very slowly (phase III) and gonadal developments rested during the winter season (phase IV). In March, the GSI values of M. oxycephalus and M. lagowskii began to increase, and reached the maximum in April (phase V). From the cyclic changes in the GSI and histological analyses, the spawning period was between mid April and mid May.

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