• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.2
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• pp.144-148
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• 1999

This study was conducted to test actual oxygen consumption rates of Nile tilapia (Oreochromis niloticus) at a commercial scale high density culture tank in the recirculating culture system, with a special emphasis on the oxygen consumption rate of this species with different daily feeding frequencies. Nile tilapia, an average of 400 g and a total wright of 390 kg, were stocked in a circular concrete tank of 2.9 m in diameter and 0.8 m in depth, in a recirculating culture system. The fish were fed commercial diet 1, 2, 5, and 9 times per day between 09:00 h and 19:00 h. feed was evenly supplied where the fish were fed more than once a day. Dissolved oxygen of influent and effluent water was monitored every 50 seconds by a computer with electrodes for 24 hours. The standard metabolic rate of tilapia was $39.31{\pm}4.4mg\;O_2/kg$ fish/hr in the present study. The maximum oxygen consumption was reached between 30 to 50 minutes after feeding. The maximum oxygen consumption levels of 1, 2, 5, and 9 feeding times per day were 161.2, 123.4, 111.1 and 111.1 mg $O_2/kg$ fish/hr, respectively. While the mean oxygen consumption levels of 1, 2, 5, and 9 feeding times per day were $79.9{\pm}21.5,\;81.3{\pm}14.8,\;84.2{\pm}9,9$ and $98,9{\pm}11.0mg\;O_2/kg$ fish/hr, respectively. These results show that oxygen consumption rates are not much different between the feeding frequencies. These results could be used to design to provide desirable oxygen supply system in the recirculating high-density tilapia culture system.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.39 no.2
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• pp.87-91
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• 2003

Experimental fishing was carried out to investigate the survival rate of fishes discarded after hauling from the trawl and to develop the fishing gear and method for fisheries management which can improve the survival rate of young fishes escaped from the trawl codend in offshore korean southern sea and off Cheju Island of Korea. The young fishes were bred in fish cage on the board to measure the sustainable survival time for fishes escaped from grid panel and codend. The obtained results are summarized as follows' 1. Japanese flying squid(Todarodes pacjficus), mitra squid(Loligo chinesis), hair-tail( Trichiurus lepturus), john dory(Zeus japonicus), spanish mackerel(Scomberomorus niphonius), redlip croaker (Pseudosciaena polyactis) and blackthroat seaperch(Doederliinia berycoides) were dead instantly after hauling. 2. Survival rate of tiger shark(Galeocerdo cuvier), conger eel (Conger myriaster), red skate (Dasyatis akajei), black scraper(Navodon modestus) and japanese fan lobster(Ibacus ciliatus) might be high after discarding because they survived for long hours in fish cage. 3. Blotched eelpout(Zoarces gili) escaped from the escapement device(grid) was dead within 6 hours in the water tank installed on the board but 97% of tiger shark and 72% of conger eel survived over 72 hours. 4. Red skate escaped from trawl codend was dead within 60 hours in the water tank installed on the board but sustainable survival time of 25% of octopus(Paroctopus dofleini) and 100% of black scraper was over 72 hours. 5. Compared with the survival rate of rosefish(Helicolenus higendorfi) escaped from the escapement device(grid) and trawl codend, all of the rosefish escaped from the grid was dead within 7.2 hours but 8.3% of the rosefish escaped from the codend survived over 72 hours. 6. In comparison with the survival rate of japanese fan lobster survived over 72 hours, survival rate of japanese fan lobster escaped the codend was much higher as 75% than 33% of japanese fan lobster escaped from the grid.

• Journal of Animal Environmental Science
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• v.15 no.1
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• pp.9-16
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• 2009

This study, tasting 14 months, was conducted to investigate the effects of different pen size and group size on growing-fattening characteristics of Hanwoo steers. Forty-eight, 12-month-old Hanwoo steers($305.8{\pm}32.2\;kg$) were randomly assigned to three groups($35.28\;m^2$; n=4 heads, $70.56\;m^2$; n=8 heads, $105.84\;m^2$; n=12 heads) and reared in separate pens with a constant space allowance of $8.82m^2$ per head from 12 to 21 month of age and then regrouped to 4 heads per pen. A common diet including concentrate(limited) and forage(ad lib) was provided to all the animals. Images of live animal ultrasonic back fat thickness, longissimus muscle area and Marbling score were evaluated in three months interval from 12 months of age using an ultrasound equipment(HS-2000) at the 13th rib and lumber vertebra interface of left side. Significant differences of ADG was found mainly at $15{\sim}18$ month and $18{\sim}21$ month fattening stages(p<0.05). Marbling score(MS) was higher(p<0.05) in 12 heads group when compared with that of 4 and 8 heads groups after 18 months. Animals in 12 heads group had the lowest Average daily gain(ADG) but showed the highest longissimus muscle area(LMA) and marbling score(MS). In addition, Hanwoo steers in 12 heads group obtained a higher quality appearance(HQA) of 82.7% than that of other treatments. The results indicated that Hanwoo steers housed on large group size and pen size decreased their ADG but improved meat quality.

• Journal of Aquaculture
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• v.9 no.1
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• pp.25-42
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• 1996

The study has been conducted to know an appropriate feeding strategy and effects of the rearing density on larval growth of the rockfish, Sebastes schlegeli. The results obtained are as fellowed ; 1. Thirty-day-old larvae reached at $25.25{\pm}3.76$ mm in total length and $0.23{\pm}0.07$ g in body weight in experiment A, at which rotifer was provided from the beginning to the end of 30-day experiment, Anemia from 3th to 18th day, and artificial feed from 13th to 30th day after hatching. When rotifer was provided for 30 days, Artemia from 6th to 18th day, and artificial feed from 18th to 30th day after hatching (experiment B), these larvae grew up to $27.52{\pm}2.50$ mm in total length and $0.26{\pm}0.06$ g in body weight. On the other hand, when rotifer and artificial feed were supplied with the same time schedule as shown in experiment B, and Artemia was feed from 6th to 30th day after hatching (experiment C), the total length and body weight of those larvae were $23.22{\pm}3.44$ mm and $0.15{\pm}0.05$ g, respectively. The best result for larval growth was obtained from experiment B. The survival rates estimated were $57.6\%$ in experiment A, $66.4\%$ in experiment B, and $44.4\%$ in experiment C. 2. The growth in total length of the larvae according to their rearing days could be represented by the following equations : Experiment A : Y=4.350+0.116X+$1.887X^2$ (r=0.993) Experiment B : Y=4.500+8.931X+$2.221X^2$ (r=0.994) Experiment C : Y=4.478+5.734X+$1.881X^2$(r=0.990) The average number of Artemia nauplius intaken by the larvae was rapidly increased between 15th and 20th day afer hatching, and 9, 212, 242, 750, and 1,171 nauplius were found in the different sizes of larvae, whose total length were 5.65, 6.81, 9.45, 14.96, and 24.52 mm, respectively. 3. Larval growth in total length and body weight reared at four different densites (A: 1.8 $kg/m^3$, B; 4.0 $kg/m^3$, C; 5.0 $kg/m^3$, D; 6.2 $kg/m^3$) indicated that the best growth was found in experiment A, at which the larval were reared at the lower density and the final survival rates extimated were $92.9\%$ in exp. A, $99.5\%$ in exp. C, $89.0\%$ in exp. B, and $88.2\%$ in exp. D. The amount of production per cubic meter turned out to be 30.45 kg in exp. D, 25.89 kg in exp. C, 20.75 kg in exp. B and 10.48 kg in exp. A. therefore, considering both larval growth and survival rate, higher yields seemed to be attainable at the relatively high-rearing density.

• Magazine of the Korean Society of Agricultural Engineers
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• v.40 no.2
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• pp.18-28
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• 1998

현재의 가축분뇨처리 형태와 축종별 적용대상은 표 3과 같이 분류되며, 축분 퇴비화 뇨오수 정화방식의 고액분리 형태에서 분뇨혼합물의 동시처리 즉, 깔감축사(소, 돼지), 슬러리 발효 퇴비화를 통한 무방류 시스템으로 변화되는 추세이다. 이러한 현상은 최근의 방류수질 규제강화에 따른 무방류화 저비용 단순화를 지향하고 있으며, 무방류화의 가장 큰 걸림돌은 톱밥과 같은 수분조절재 부족이라 할 수 있다. 현재 이용되고 있는 주요 시스템별 개선안은 다음과 같다. $\square$ 주요 처리 시스템 적용의 문제제기 $\circ$ 축분발효시설 - 톱밥 등 부자재 무이용을 위한 축분의 예비 건조시설 이용 - 교반식 발효기의 악취확산방지를 위하여 밀폐형 하우스 및 강제환기 장치 설치에 의한 악취 포집 및 탈취 - 중소규모 발효시설 개발 $\circ$ 화력건조 - 예비건조를 통한 톱밥, 연료비용 절감 - 악취방치 장치부착 의무화 $\circ$ 활성오니 정화시설 - 시설 운전관리 단순화 - 1차 고액분리를 통한 유입수 오염부하량 감소 및 균일화 $\circ$ 깔감축사 - 경제적으로 흡수를 극대화할 수 있는 수분조절재의 조합비 결정 및 혼합물을 깔감으로 재이용 기술개발 $\circ$ 슬러리 발효퇴비화 시설 - 톱밥 등 수분조절재 사용량 절감기술 - 증발량 극대화 기술개발 및 소요에너지 최소화기술개발 우리나라는 사계절이 뚜렷하여 외기상에 따라 편차가 심하며, 고밀도 사육 및 지역적 편중성, 경지면적 협소, 고비용 시장구조 등의 축산환경을 고려한다면 근원적으로 가축분뇨문제 해결한다는 것은 결코 쉬운 일이 아니다. 이는 저공해 사료개발에서부터 분뇨가 발생단계에서 분리, 수거할 수 있는 수거시설, 고효율 분뇨처리시스템의 정립, 액비 및 퇴비의 가공, 토양환원되었을 때 작물생장장애, 액비, 퇴비의 유통, 가축분뇨처리 정책 및 규제법 등의 각 분야에서 복합적 노력이 필요하다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.4
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• pp.187-194
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• 1974

When rearing fish at recirculating filter systems, some pesticides are often used for controling various fish diseases. In this case, the pesticides are usually applied after stopping the filteration, from a view point of affecting the filtering ability of oxidation and decomposition by killing the decomposing bacteria in the filter system. Therefore, a great inconvenience is usually net. The authors carried out a series of experiments, and the influence of 3 kinds of treatments of pesticides, formalin 33 ppm+matachite green 0.15 ppm, dipterex 0.25 ppm, and furanace 0.1 ppm, to the filtering ability expressed as the consumption rate of dissolved oxygen and depletion of ammonia-nitrogen during the passing time through the filter layer was investigated, and it was found out that these pesticides with above mentioned concentrations which is normally used for controling several kinds of diseases and parasites during the time of eel culture, did not affect the filtering ability. Consequently, it can be said that these pesticides with above mentioned concentrations can he used without stopping filteration.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2013.11a
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• pp.143-143
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• 2013

대학, 기업 및 국 공립 출연 연구소의 연구 활동 종사자는 학술적 연구 및 신기술, 신물질의 개발을 통해 우리나라 과학기술 발전에 크게 기여하고 있다. 그러나 이와 더불어 각종 안전사고에 노출되어 있는 것도 사실이다. 즉, 실험실은 대부분의 작업장 보다 더욱 많은 화재, 폭발 등의 위험에 항상 노출되어 있다. 이는 일반적인 작업장과는 다르게 실험이 종료되면 기존 실험 장치를 철거하고 신규로 다른 실험장치가 설치되기 때문이다. 연구실험실에서 발생한 사고 원인을 분석해 보면, 실험실 기능의 불비, 설비 및 유지관리의 불충분, 실험공간의 협소, 실험 작업 안전에 대한 배려부족, 안전전문가의 부족과 교육훈련체제의 미흡 등을 지적할 수 있으며, 이와 같은 빈번한 사고발생은 우수한 연구 활동 종사자의 연구 의욕을 떨어뜨리고, 건강과 생명에 위협을 느끼게 하고 있다. 본 연구에서는 기존의 실험실 안전관리 체크리스트들을 검토하고, 안전관련 분야에서 사용되고 있는 심의 체크리스트를 조사하여, 대학 및 연구기관 등의 바이오실험실에 사용할 수 있는 표준 체크리스트를 개발하고자 한다. 현재 개발된 바이오 분야 체크리스트는 "연구실험실 공통 체크리스트"에 기반하여 바이오 분야에서 중점 평가할 항목을 심화시켜 구성하였다. 바이오 분야에서 주로 이루어지는 생물 실험 및 동물해부 실험시 안전을 위해 반드시 점검해야 할 총 8개의 세부 점검 항목을 제시하였으며, 실험동물 관리 및 사육실 출입시 안전을 위해 점검해야 할 총 11개의 세부 점검 항목을 제시하였고, 세포, 조직 배양 실험 및 유전자 조작 실험시 안전을 위해 점검해야 할 총 5개의 세부 점검 항목을 제시하였다. 또한 미생물 취급시 안전을 위해 점검해야 할 총 19개의 세부 점검 항목을 제시하였으며, 세균 취급시 안전을 위해 점검해야할 총 8개의 세부 점검 항목을 제시하였고 마지막으로, 바이러스 취급시 안전을 위해 점검해야할 총 5개의 세부 점검 항목을 제시하였다. 실험실 안전은 연구종사자의 올바른 안전의식, 연구관련 부서의 체계적 지원 및 실험실 안전 관련 법령의 완비가 뒷받침 되었을 때 비로소 가능한 일이다. 따라서, 본 연구에서 얻어진 체크 리스트가 이에 기여할 것이라 사료되며, 추후 분야별 실험실 안전관리 실태조사의 지침서로 활용되는데 목적이 있다.

• Journal of Aquaculture
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• v.1 no.1
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• pp.67-73
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• 1988

A growth experiment of tilapia (offsprings of the hybrid between Oreochromis niloticus and O. mossambicus) under different dissolved oxygen levels in the recirculating water system was conducted at the Fish Culture Experiment Station of the National Fisheries University of Pusan from February 4 to March 5, 1986. Six tanks with a capacity of $1.8m^3$ of water each were used under the same condition of water parameters except for dissolved oxygen levels which were designated to maintain at 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0 mg/$\iota$. Each tank was stocked with 90 kg of fish each averaging 64 to 69 grams. The average water temperature during the course of the experiment was $22.5^{\circ}C$. The results obtained are summarized as follows: The food conversion efficiencies were very good, being 1.05-1.11 at 3.5, 3.0, 2.5 and 2.0 mg/$\iota$ DO levels without any significant differences among them, but at 4.0 mg/$\iota$ the F. C. was 1.39 and at 1.5 mg/$\iota$ it was 1.61 being very poor compared with the others. The daily growth rate performance was best at 3.5 mg/$\iota$ dissolved oxygen level followed by 3.0 and 2.5 mg/$\iota$ with slight differences while at 4.0 and 2.0 mg/$\iota$ DO levels the growths were significantly poor, and at 1.5 mg/$\iota$ DO level it was extremely poor. In 1.5 mg/$\iota$ group, the fish did not accept feed vigorously and after feeding the fish usually concentrated around the inflow point showing oxygen deficiency response. While at 4.0 mg/$\iota$ high feeding rates tended to waste significant amounts of feed while eating and led to water deterioration, and above these levels the results is considered to lead to a waste of energy with uneconomical performance. On the other hand, at and below 2.0 mg/$\iota$ DO level the tilapia certainly showed a poor growth performance. The experiment indicates that the DO range of 2.5$\~$3.5 mg/$\iota$ is the optimum level for the good growth performance.

• Journal of fish pathology
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• v.19 no.3
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• pp.253-265
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• 2006

The purpose of this study was to obtain reference data of parameters for hematological health diagnosis in marine fish and also evaluate application of veterinary chemistry analyzer used to those blood tests. A blood profile of total 522 fish for black rockfish (Sebastes schlegeli), red seabream (Pagrus major), rock bream (Oplegnathus fasciatu) and black seabream (Acanthopagrus schlegeli) cultured in mari-floating netcage of Gyeongnam province was determined by hematocrit (Ht), hemoglobin (Hb) and blood chemistry tests (total protein, albumin, alkaline phosphatase, blood urea nitrogen, lactate dehydrogenase, triglyceride, total cholesterol, creatinine, aspartate aminotransferase, alanine aminotransferase, glucose). Ht was measured by microhematocrit method. Hb and plasma chemistry were analysed by establishing baseline ranges for a dry chemical system of FUJI DRI-CHEM 3000. Actually recorded values of Hb and plasma chemistry by the analyzer were notably outside from the minimum and/or maximum of the established reference value. Albumin and alanine aminotransferase were not detectable in the range of 68～66%. Lactate dehydrogenase, total protein, alkaline phosphatase and glucose were not detectable in the range of 42～21%. Total cholesterol, aspartate aminotransferase, triglyceride, hemoglobin and creatinine were not detectable in the range of 18～3%. However, the values of blood urea nitrogen were below the detectable limits of the analyzer.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.6
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• pp.498-504
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• 2004

Removal of waste generated by Paralichthys olivaceus in the seawater aquarium using a foam separator was investigated. Protein concentration without a foam separator continuously increased until 3 days after stocking and reached at 25 mg/L after 5 days stocking, but protein concentration became lower than the initial protein concentration (2.5 mg/L) with a foam separator. The trends of other fish wastes such as ammonia, total suspended solids (TSS) and chemical oxygen demand (COD) were similar to protein. Dissolved oxygen (DO) in the aquarium decreased below 6.0 mg/L without a foam separator, but with a foam separator the average DO in the aquarium was 7.3 mg/L. Foam separation with the increase of superficial air velocity (SAV) was more effective than that with the fixed SAV. This study showed that wastewater. treatment of seawater aquarium using a foam separator is effective method for a fish waste removal and oxygen supply.