• Applied Biological Chemistry
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• v.27 no.2
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• pp.86-94
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• 1984

The changes of the volatiles from apple fruits were examined with Mallus pumila Miller var. Fuji, Ralls Janet and Jonathan harvested at the preclimacteric stage and stored under the normal atmospheric pressure of 760mmHg and subatmospheric pressure of 380mmHg at the temperature of 1 and $20^{\circ}C$ each. The production of the volatites in the normal storage temperature increased markedly up to around 30 days on storage and then decreased rapidly, whereas that of the volatiles in the low storage temperature increased slowly before decreasing. The changing pattern of volatiles from apples during storage at the normal atmospheric pressure was more remarkable than that of volatiles produced at the subatmospheric pressure. During storage, most of flavoring materials were increased, and the decrease of the content of esters was more rapid than that of alcohols and aldehydes among the volatiles. The amounts of the volatiles in Fuji apples during ripening at $20^{\circ}C$ after removing from the subatmospheric pressure storage for 90 days at $1^{\circ}C$ were nearly maximum at 3 days on ripening.

• Journal of Korean Society of Forest Science
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• v.103 no.2
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• pp.307-312
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• 2014

Wild boar have increased in recent years in Korea where damage to agricultural crops is considered as a social issue. The ecological studies on wild boar are essential for the effective management in the future. During May and December 2012, We collected 79 wild boar stomach samples from culling program implementation to reduce agricultural damage in Geochang County and analyzed stomach contents to figure out wild boar diet habit and which crop was preferred. There was seasonal variation in the diet. Plant foods represented up to 93.5% of the diet and were consumed more frequently than animal foods. Invertebrates occurred with high frequency but were underestimated in dry weight because they are rapidly digested. Agricultural crops consumed comprised mainly chestnuts(33%), rice(33%), and apples(24%). Agricultural crops are also a potentially important food resources for wild boar. The frequency of occurrence of earthworms was 50% of diet. Earthworm consumption generally increased in summer, and then decreased in winter. Our results show that wild boar can be considered an omnivore whose diet consists of plant and animal foods. To reduce wild boar agricultural damages, We have to focus on the prevention effort to rice paddy adjacent to chestnut forest and to apple orchard.

• Korean Journal Plant Pathology
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• v.1 no.2
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• pp.128-135
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• 1985

Responses to the 14 fungicides added in PSA of penicillium expansum isolated froms the four major apple growing areas in Korea were examined. Degrees of the fungal tolerance were various with little difference by area and with great difference by kind and concentration of fungicide. Fall isolates of the fungus grew at higher fungicide concentration than did the spring isolates. $ED_{50}$ and MIC to mycelial growth of genomyl, garbenda, polydong, polyoxin and thiophanate methyl were $0.1{\mu}g-41.7{\mu}g/ml\;and\;100{\mu}g-2,250{\mu}g/ml,$, respectively, indicating high fungicide effects on the fungus isolated from decayed apples in storages and infected apples in orchards with low fungal tolerance; of captafol, captan, iprodione and mancozeb were $2.6{\mu}g - 750{\mu}g/ml\;and\;638{\mu}g-40,500{\mu}g/ml$, respectively, indicating medium degree of both fungicidal effects and fungal tolerance; and of chlorothalonil, folpet, oxidong, propineb and triademefon were $27.8{\mu}g-8,354{\mu}g/ml\;and\;2,625{\mu}g-150,357{\mu}g/ml$, respectively, indicating low fungicide effects and high fungal tolerance. Fungicidal inhibitory effects on conidia formation exhibited a similar trend as on mycelial growth whereas those on conidia germination showed an opposite trend to the mycelial growth with the exception of polyoxin and triademefon.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.552-560
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• 2010

$GA_{4+7}$+BA, a plant growth regulator for induction of feathering in young apple tree and increasing fruit size, was applied by various methods on 'Gala'/M.9 apple trees in high density orchard for 4 years to investigate its effect on fruit and shoot growth. $GA_{4+7}$+BA($80-300mg{\cdot}L^{-1}$) increased fruit length, fruit weight, and L/D ratio regardless of application methods, but it did not affect soluble solid content, acidity, leaf area, and chlorophyll. Seed number was not affected by $GA_{4+7}$+BA application, however, more immature seeds was observed in treated 'Gala' fruit. Shoot growth was increased when spraying $GA_{4+7}$+BA at tree crown but not affected when spraying at fruit directly. More significant fruit growth was observed when $GA_{4+7}$+BA was applied on the fruits between late of May and early of June when fruit cell division ended; however, high concentration of $GA_{4+7}$+BA resulted in lower fruit storability because of lower firmness. Hence, more attention should be paid when applying high concentration of $GA_{4+7}$+BA to small sized fruit cultivars like 'Gala'.

• Journal of the Korea Organic Resources Recycling Association
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• v.28 no.3
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• pp.5-14
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• 2020

This study aims to survey the effect of sulphuric acid (H₂SO₄) treatment for pH adjustment with 6.5 and 7.0 regarding ammonia volatilization on chemical content change in the aerobic liquefying process of co-digesate swine manure and apple pomace. The digestates of swine manure was aerated with 0.3 ㎥ air/㎥·min for 60 days. The untreated digestate showed the increased pH and decreased contents of electrical conductivity (EC) and total nitrogen (T-N). The untreated digestate had a high concentration of NH₃ with 172.6 mg/L, but, ammonia (NH₃) concentration of H₂SO₄-treated digestate was significantly lower than that of untreated digestate. The H₂SO₄-untreated digestate for retaining aeration showed a decreased concentration of 47.2% of ammonium nitrogen. While, the H₂SO₄-treated digestate had a high concentration of ammonium nitrogen compared to the untreated digestate. Also, the H₂SO₄ treatment affected to increase the contents of nitrogen and phosphoric acid content. Therefore, the pH adjustment with H₂SO₄ might be a useful method for the decreased ammonia concentration and nitrogen maintenance in the aerobic liquefying process of swine manure digesate.

• The Korean Journal of Pesticide Science
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• v.13 no.3
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• pp.185-189
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• 2009

A monoterpenoid compound, benzylideneacetone (BZA), is a metabolite of an entomopathogenic bacterium, Xenorhabdus nematophila. Its primary biological activity is an inhibitor of phospholipase $A_2$, which catalyzes the committed step of biosynthesis of various eicosanoids that are critically important to mediate insect immune responses. When BZA was applied to two-spotted spider mite, Tetranychus urticae, it exhibited a dose-dependent mortality in leaf-disc assay. Subsequently BZA was tested against T. urticae infesting apples in a field orchard, in which it showed a significant control efficacy, which was not statistically different with that of a commercial acaricide. BZA also had significant antibacterial activities against three species of plant pathogenic bacteria when it was added to the bacterial cultures, in which it showed the highest inhibitory activity against a bacterial wilt-causing pathogen, Ralstonia solanacearum. The bacterial pathogen caused significant disease symptom to young potato plants. However, BZA significantly suppressed the disease occurrence. This study suggests that BZA can be used to develop a novel crop protectant to control mite and bacterial pathogen.

• Korean journal of applied entomology
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• v.56 no.1
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• pp.61-67
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• 2017

Two major sex pheromone components (Z-8-dodecenyl acetate and E-8-dodecenyl acetate) are known in the peach fruit moth, Grapholita molesta. From a putative biosynthetic pathway of these sex pheromone components, delta 10 desaturase ($10{\Delta}$ DES) has been proposed to play a crucial role in synthesizing a species-specific stereoisomer of the double bond. However, its molecular identity was not known. This study determined a putative desaturase (Gm-comp1575) as a $10{\Delta}$ DES candidate from G. molesta transcriptome constructed from the sex pheromone gland. Its open reading frame encodes 370 amino acid sequence with a predicted molecular weight at 43.2 kDa and isoelectric point at 8.77. It was predicted to have four transmembrane domains and six glycosylation sites at N-terminal or cytosolic domains. A phylogenetic analysis with its predicted amino acid sequence indicated that Gm-comp1575 is closely related with known $10{\Delta}$ DES genes of other insects. Gm-comp1575 transcript was detected in female adults at sex pheromone gland and other abdominal tissues. RNA interference of Gm-comp1575 significantly reduced attractiveness of virgin females in apple orchard compared to control females. These results suggest that Gm-comp1575 is associated with sex pheromone biosynthesis of G. molesta.

• Korean journal of applied entomology
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• v.35 no.3
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• pp.260-265
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• 1996

A field colony of the two-spotted spider mite, Tetranychus urticae Koch, was selected with tetradifon for 4 mousing whole-plant residual method. This strain showed 371-fold resistance to tetradifon. The log dosehatchability lines of $F_{1}$ eggs(RS cross; Td5female$\times$Smale, and SR cross; Sfemale$\times$Td5male) were closer to the line of the resistant colony than to that of the susceptible. These differences could be due to cytoplasmic inheritance or maternal effect. The estimate of dominance index (D) for the $F_{1}$ eggs of RS cross was 0.998 and that for $F_{1}$ eggs of SR cross was 0.262. This indicates that tetradifon resistance is completely dominant in RS cross and incompletely dominant in SR cross. Td5 strain exhibited high levels of resistance to clofentezine, benzoximate, and chlorfencon, and no cross resistance to fenazaquin, pyridaben, flufenoxuron, tebufenpyrad, and fenothiocarb.

• Research in Plant Disease
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• v.10 no.2
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• pp.145-149
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• 2004

'Hongro' is one of the most important apple cultivars whose growing area is increasing because of its good quality. Recently fruit shrinking symptom causing decrease of fruit size, juice, and quality, appears in some commercial 'Hongro' orchards. The average frequency of occurrence of fruit shrinking symptom was 12 % of total trees investigated and Apple chlorotic leaf spot virus (ACLSV) was detected from all the trees showing fruit shrinking symptom by ELISA. A typical virus infection symptom of leaf epinasty and stem necrosis appeared on woody indicators, Spy227 and Virginia crab grafted with infected trees and all the grafted trees showed positive reaction to ACLSV antiserum by ELISA. It was proved that ACLSV can be easily transmitted by grafting. ACLSV was also isolated from the leaves of C. quinoa inoculated with sprouting leaf sap of infected trees. To prove that the fruit shrinking symptom was caused by ACLSV infection, ACLSV-infected scion was grafted on virus-free 'Hongro/M9' and the fruit characteristics were investigated. Consequently the same symptoms of fruit size and juice decreasing were observed from the trees grafted with ACLSV-infected scion. Therefore, it is suggested that the fruit shrinking symptom is caused by ACLSV infection and 'Hongro' can be classified as sensitive cultivar to ACLSV.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.310-317
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• 2019

Plant regeneration protocols for adventitious shoot organogenesis from apple (Malus domestica 'Fuji') leaf explants were developed in the present study. The effects of different basal media, types and concentrations of carbon sources, and concentrations of plant growth regulators were evaluated to determine the optimal shoot regeneration conditions for 'Fuji' apple leaf explants. On different treatments involving combinations of basal media, LS and N6 media, and different types and concentrations of cytokinins, 6-benzyl-adenine (BA) and thidiazuron (TDZ), shoot regeneration rates were the highest in the N6 medium combined with BA. Among the plant growth regulator and carbon source combination treatments, 5.0 mg/L BA, and 0.1 mg/L α-naphthalene acetic acid (NAA) with 40 g/L sorbitol was the optimal combination for shoot regeneration. In addition, the optimal sorbitol concentrations for shoot regeneration were 40 g/L and 60 g/L. The highest regeneration (81.8%) was achieved using 40 g/L sorbitol. The regenerated shoots elongated and rooted on rooting medium, consisting of 1/4 MS medium with 0.2 mg/L indole-3-butyric acid (IBA). The plantlets were acclimatized and the regenerated plants exhibited normal phenotypes.