• Journal of agriculture & life science
• /
• v.43 no.6
• /
• pp.105-109
• /
• 2009

Kyungsan nursery complex which has a vast area for the production of various species of fruit tree stocks is in a high demand of virus-free saplings. Apple tree stocks, the most important products, urgently need more rapid and reliable viral diagnosis. In this study, a bead beater was tested because of convenience in dealing with large number of samples. Also, industrial glass bead abrasive (0.4 mm in diameter) at very low cost was used in a disposable way. For bead beater-aided RNA extraction from apple stem tissues, the guanidine thiocyanate method was confirmed to be very reliable. Silca membrane filter tube in connection to vacuum filtering device was strongly suggested for simplifying RNA capture and washing steps. Apple virus detection was confirmed by RT-PCR.

• Korean Journal of Plant Resources
• /
• v.32 no.5
• /
• pp.499-508
• /
• 2019

The study was carried out to investigate growth of 48.6-L pot-cultivated 1-year-old 'Fuji'/M.9 apple trees depending on different levels of nitrogen concentration. While rise in tree growth was paralleled with increase of nitrogen concentration, more than 32 mM of nitrogen rather restrained tree growth. In particular, growth of 16 mM of nitrogen treated trees was satisfied with criteria for production of high-quality pot-cultivated nursery stocks. Although mineral contents of leaves were higher in 8 and 16 mM nitrogen treatments than commonly recommended mineral contents in apple orchards, such somewhat surplus minerals could be helpful for tree growth after transplanting to apple orchards. In addition, our result indicated that soils of 8 and 16 mM of nitrogen treated pots met appropriate criteria for soil chemical property of apple orchards. Thus, in the light of tree growth, mineral contents of leaves, and soil chemical property in the pots, 16 mM of nitrogen treatment is considered to be suitable for production of 1-year-old 'Fuji'/M.9 apple potted trees.

• Horticultural Science & Technology
• /
• v.19 no.1
• /
• pp.39-42
• /
• 2001

C6-Benzylamino purine (BAP) 400 ppm with 500 ppm Spreader, 500 ppm Cover, 250 ppm Spreadersticker, 350 ppm Siloxane, or 1% Tween 20 was sprayed and the effect of surface-active agents on the feathering of maiden apple trees was evaluated. Only Tween 20 showed additional effect on the feathering in comparison with the single BAP treatment. In the second year different concentrations of Tween 20 were added to 400 ppm BAP. Each BAP treatment added with 0.2, 0.5, 0.8, or 1.0% Tween 20 was more effective in feathering than BAP 400ppm alone, but no significant differences among the concentrations.

• Landscaping Tree
• /
• s.144
• /
• pp.66-67
• /
• 2015

몇 년 전 제주도의 소나무 묘포장에서 급격히 생장이 떨어지다가 결국 나무가 죽는다는 피해 진단 의뢰가 있어 현지 조사를 하였다. 병원균을 동정(同定)한 결과 자주날개무늬병(Helicobasidium mompa)에 의한 피해로 밝혀졌다. 자주날개무늬병은 과수, 뽕나무, 느릅나무, 은행나무, 참나무류, 편백나무 등 넓은 기주범위를 가지며 특히 사과나무에 큰 피해를 주는 것으로 알려져 있다. 우리나라에서는 소나무에 공식적인 보고기록은 없으나 본병과 발생지의 특성을 파악하여 소나무 묘목관리에 주의를 요하는 의미에서 이 병을 소개한다.

• 농업기술회보
• /
• v.42 no.7 s.501
• /
• pp.39-44
• /
• 2005

2기 도시민 웰빙농사교육 호응/"도시민 웰빙농사" 수강소감/중국, 흑룡강성 동포 농촌간부 및 농촌지도자 협회방문/본회 조춘선,유승윤 2명의 지도위원 별세/ 사과 고품질 및 피롤농업 세미나 개최/제주 문시병,진천 이문희, 임실 이재연 3명 회우 조합장 당선/복분자·뽕나무(오디)·땅콩의 묘목 및 씨앗분양안내/초대 경 기도지부장 이영순 선생 방문

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.13 no.1
• /
• pp.1-9
• /
• 2011

This study was conducted to investigate the influence of different polyethylene film (P.E. film) for mulching on the changes in soil temperature and the production of good feathered apple nursery trees. M.9 rootstocks with stem diameter of 9.1~11.0 mm were planted in plots covered with different P.E. film (i.e., transparent P.E. film, black P.E. film, and bare soil as control). Three weeks after planting, the rootstocks were veneer grafted with 'Sinano Sweet' apple cultivar. In the middle of June, BA was sprayed to nursery for inducing feathering during the growing season. The soil temperature of the control was higher than air temperature by about $0.7^{\circ}C$ from mid April to early October, and that of P.E. film mulching was about $1{\sim}5^{\circ}C$ higher than that of the control. The soil temperature under transparent P.E. film was about $2{\sim}3^{\circ}C$ higher than that under black P.E. film. The diurnal range of soil temperature under the black P. E. film was lowest among all treatments. The P.E. film mulching induced better tree growth and feathering than bare soil. Percentage of good feathering apple nursery of black P.E. film was highest among all treatments because the soil temperature unuder black P.E. film in the early growing season was higher than that of the control and the number of days when the maximum soil temperature was over $35^{\circ}C$ in the summer was lower than that under the transparent P.E. film.

• Horticultural Science & Technology
• /
• v.19 no.3
• /
• pp.352-357
• /
• 2001

This experiment was carried out in order to develop some chip budding techniques related to the production of high quality maiden apple trees. Optimum chip budding time for apple trees appeared to be from mid-August to early September. Untying wrapping tapes after 6 8 weeks from budding led to higher graft success and better shoot growth than removing the tapes earlier or later than the 6-8 weeks. Dipping pre-cut bud chips into ordinary water of $25^{\circ}C$ for 5 hours did not hamper the graft-success nor the early season growth of the graft compared with non-treated control. Dipping the pre-cut bud-chips into the $35^{\circ}C$ water for 3 hours or for 5 hours hampered the union formation and shoot growth of the 'Fuji' apple trees.

• Horticultural Science & Technology
• /
• v.17 no.4
• /
• pp.476-480
• /
• 1999

This study was carried out to determine the optimum storage temperatures of six fruit nurseries ('Fuji' apple, 'Niitaka' pear, 'Sheridan' grape, 'Yumyoung' peach, 'Fuyu' persimmon, and 'Hayward' kiwifruit) from 1995 to 1997. Nursery plants were planted in field after storage at -5, 0, and $5^{\circ}C$ for one year. Nurseries stored at $0^{\circ}C$ showed highest survival rate and growth after planting. The survival rate of 'Sheridan' grape and 'Hayward' kiwifruit nursery plants stored at $-5^{\circ}C$ was 0%. Cold resistance of the two fruit nursery plants appeared weaker than others. Also, 'Niitaka' pear nursery plants stored at $5^{\circ}C$ showed significantly high survival rate and growth after planting. The higher storage temperature, the lower mineral and carbohydrate contents of nursery plants. Therefore, $0{\sim}5^{\circ}C$ in 'Niitaka' pear and near $0^{\circ}C$ in other five fruit nursery stocks were found to be the optimum storage temperatures.

• Journal of Plant Biotechnology
• /
• v.49 no.3
• /
• pp.222-229
• /
• 2022

Apples are the most grown fruit crops in the fruit industry of Korea. However, virus or viroid infection such as apple mosaic virus (ApMV), apple stem grooving capillovirus (ASGV), apple stem pitting virus (ASPV), apple chlorotic leaf spot virus (ACLSV), apple scar skin viroid (ASSVd) causes fruit yield reduction and poor fruit quality. Therefore, in this study, we examined to established an efficient virus-free system to eliminate the most infected ASGV virus in domestic apple orchard. We investigated that the shoot growth rate and the virus removal rate in ASGV infected potted apples that were treated with heat treatment in a growth chamber (constant temperature/humidity device) maintained at 36℃, 38℃ and 40℃ for 4 weeks. Here we found that the shoot growth rate was the highest in the heat treatment group (36℃) and the virus was removed in the middle and top of the shoot but not in the bottom. The virus was did not removed in the 38℃ and 40℃ heat treatment group in all section of shoots, and the heat treatment group (40℃) died after 4 weeks of heat treatment without growth of shoots. We performed in vivo shoot-tip grafting using the shoot-tip of potted apple heat-treated at 36 ℃, and we also investigated the viability and virus removal rate, which showed 94% viability and 20% virus removal rate. Collectively, our results suggest that it would be possible to produce the virus-free apple plants through heat treatment and shoot-tip grafting.

• Korean journal of applied entomology
• /
• v.43 no.3 s.136
• /
• pp.189-194
• /
• 2004

This study investigated the species of leafrollers in apple orchards and nursery farms in Gyeongsangbuk-do for three years from 1998 to 2000, and also examined the occurrence and damage of leafrollers in commercial apple orchards in 5-6 cities and districts for 10 years from 1992 to 2001. Total seven species of tortricidae were found in the apple orchards, which were Adoxophyes orana, Archips breviplicanus, Rhopobota unipunctana, Choristoneura longicellana, Acleris fimbriana, Ptycholoma lecheana circumclusna and Archips subrufanus. Among them, A. orana was dominant species every year, and A. breviplicanus and R. unipunctana occurred with a high density in one or two farms in some years. The dominant species in the 1980s were A. breviplicanus and R. unipunctana, but it was considered that dominant species have been changed in the late 1990s. According to the result of leafroller damage in commercial apple orchards for 10 years, the mean fruit damage rate was $0.67\%$. Fruit damage was observed frequently between August and October by third-generation larvae.