• Title/Summary/Keyword: 뿌리 생장

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Effect of Light on Root Growth and Gravitropic Response of Phytochrome Mutants of Arabidopsis (Arabidopsis phytochrome mutant에서 빛이 뿌리 생장과 굴중성 반응에 미치는 영향)

  • Park, Ji-Hye;Lee, Sang-Seoung;Woo, Soon-Hwa;Kim, Soon-Young
    • Journal of Life Science
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    • v.22 no.5
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    • pp.681-686
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    • 2012
  • Light, one of the environmental stimuli, is fundamental to the growth and development of plants. Red and far-red light are sensed using the phytochrome family of plant photoreceptors. To investigate the effect of light on root growth and gravitropism, we used the Arabidopsis phytochrome mutants grown in several light conditions. The root growth of $phyA$ reared in all light conditions except white light and was stimulated compared to the WT. The stimulation of root growth was obvious in $phyA$ grown in red light. On the other hand, the root growth of $phyB$ grown in all light conditions decreased, and the lowest rate of decrease was observed in $phyAB$ grown in white and red light. The gravitropic response of $phyA$ was stimulated compared to the WT when it was grown in all light conditions except far-red light. $PhyAB$ grown in all light conditions showed the inhibition of gravitropic response. The transcript level of ACS, one of the enzymes regulating ethylene biosynthesis, increased in $phyA$ grown in white and red light, but not in $phyA$ grown in far-red light. In conclusion, these results suggested that the $P_{fr}$ form of $phyB$ regulates the root growth and gravitropism.

참당귀 뿌리 배양에 있어서 root segmentation과 식물생장조절제가 뿌리 생장과 decursinol angelate 생산에 미치는 영향

  • Kim, Ji-Yeon;Jo, Ji-Suk;Jo, Jong-Mun;Lee, Yong-Il;Kim, Ik-Hwan;Kim, Dong-Il
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.305-308
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    • 2002
  • Decursinol angelate, a new anticancer agent, was produced by root cultures of Angelica gigas Nakai. In addition, difference of specific yields between primary and secondary root was investigated. It was found that specific yield of secondary root was much higher than that of primary root at various conditions, so that it was thought that the formation and growth of secondary root were feasible. From this point of view, effects of root segmentation and plant growth regulators (NAA, IBA) on root morphology and decursinol angelate production were examined. Root segmentation increased secondary root mass and product formation. On the other hand, addition of NAA or IBA at various concentrations promoted secondary root formation and production of decursinol angelate significantly. Five-fold increase of production was obtained at 4 mg/L of IBA compared to control without NAA and IBA.

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Determination of Antifungal Activity on Pepper Anthracnose and Plant Growth Promoting Activity of Pleurospermum camtschaticum Root Extract (누리대 뿌리 추출물의 고추 탄저병에 대한 항균 및 생장 촉진 활성 검정)

  • Inkyu Lee;Young Sun Baek;Youn Su Lee
    • Research in Plant Disease
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    • v.29 no.3
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    • pp.268-275
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    • 2023
  • This study was conducted to confirm the utilization of Pleurospermum camtschaticum root extract as an organic agricultural material. Antioxidant activity of P. camtschaticum root extract, closely related to antibacterial activity, increased in a dose-dependent manner. In mycelial growth inhibitory activity, 100% P. camtschaticum root extract supressed over 70% for Colletotrichum coccodes and over 68% for Colletotrichum dematium. In the pepper fruit anthracnose development test, the size of the lesion decreased in a dose-dependent manner, which showed the same tendency as the previous results in inhibitory activity on mycelial growth. In the pepper seed germination and red pepper growth promotion test of P. camtschaticum root extract, oposite results was confirmed. The lower the concentration, the more the seed germination and growth promotion effects were shown. The phenol content of pepper leaves was also measured after pepper growth promotion test have been completed. The phenol content related to antibacterial activity increased in all treated groups compared to the untreated group. Therefore, the results of this study showed the possibility of development as an organic material.

Studies on the Allelopathy of some Poaceae Plants (수종 화본과식물의 Allelopathy에 관한 연구)

  • Lee, Kyung Soon;Il Koo Lee
    • The Korean Journal of Ecology
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    • v.4 no.3_4
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    • pp.93-108
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    • 1981
  • This investigation includes the sick soil phenomenon caused by the self-poisoning of Setaria italica, Sorghum nervosum, Zea mays and Miscanthus sinensis among Poaceae. It elucidates whether the poison is directly excreted from the root or the secondary product resulting from the decomposition in the soil; the effect of Miscantus sinensis on the germination and growth of other plants, and the effect of Zea mays grown between furrows to shade Angelica gigas on its growth. The results obtained are as follows; Supplied with the leakage water from the pots, in which the same plants as the test ones were grown, in anticipation of the poison to be directly excreted from their roots, Sataria itlaica and Zea mays exhibited the growth inhibition more than 30%, whereas Sorghum nervosum and Miscanthus sisnensis were not effected in growth at all. When cultivated in the soils mixed with the roots of the some plants as the test ones, in anticipation of the poison to be the secondary product resulting from the decomposition in soil, Setaria italica and Zea mays showd growth inhibition of more than 50%, which is greater than that of the case of the leakage water, and Miscanthus sinensis exhibited no inhibition either, whereas Sorghum nervosum in the 50% plot showed heavy growth inhibition of more than 80% to the case of the leakage water. The common or uncommon plants found easily in the group of Misscanthus scinenis were not affected by the extracts of the steam and leaves of Miscanthus sinensis in germination and growth. Supplied with the leakage water from the pots in which Miscanthus sinensis was grown, among Lespedeza crytobotrya, Oenothera odorata, Raphanus sativus val'. acarlthiformis, Zoysia japonica, Patrinia scabiosaefolia. which are easily found in the group of Miscanthus sinensis, only Patrinia scabiosaefolia was slightly inhibited in growth in the 100% plot, whereas the others did not show any inhibition at all. Mean while, Amaranthus patulus. Solanum nigrum, Capsella bursa-pastoris val'. triangularis, Alopecurus amurensis, Chenopodium album val'. centrorubrum, which could not be found in the group of Miscanthus sinensis, were all distinctly inhibited. In the experiment on the effect of Zea mays on the growth of Angelica gigas, its growth was severely inhibited by one-half to two thirds with the increased concentration in both the cases of growing in the mixture of the soil and the powdered root of Zea mays and being supplied with the leakage water from the pot in which Zea mays was grown.

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Effect of Northern Root-knot Nematode, Meloidogyne hapla, on Growth of Codonopsis Zanceolata (당근뿌리 혹선충(Meloidogyne hapla)이 더덕(Codonopsis lanceolata)의 생육에 미치는 영향)

  • Jung Do-Chul;Han Sang-Chan
    • The Korean Journal of Soil Zoology
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    • v.8 no.1_2
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    • pp.27-31
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    • 2003
  • This study has been conducted to assess and analyze the damage of Codonopsis lanceolata by differential inoculation levels of Meloidogyne hapla in pot and plot. As the density of M. hapla increased, early growth of C. lanceolata was inhibited 60% in vine and 54% in root. However, growth of C. lanceolata was not remarkably different by density of M. hapla in the plot test. This result may be attribute to low density of M. hapla in the plot.

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The Effect of Oryzalin on Growth and Gravitropism in Arabidopsis Roots (Oryzalin이 애기장대 뿌리 생장과 굴중성 반응에 미치는 작용)

  • Go, Jin Gyu;Park, Sun Ill;Kim, Soon Young
    • Journal of Life Science
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    • v.31 no.2
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    • pp.109-114
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    • 2021
  • Oryzalin is a dinitroaniline herbicide that has been known to disrupt microtubules. Microtubules and microfilaments are components of cytoskeletons that are implicated in plant cell growth, which requires the synthesis of cellulose when cell walls elongate. In addition, microtubules are also involved in the sedimentation of statoliths, which regulate the perception of gravity in the columella cells of root tips. In this study, we investigated the effect of oryzalin on the growth and gravitropic response of Arabidopsis roots. The role of ethylene in oryzalin's effect was also examined using these roots. Treatment of oryzalin at a concentration of 10-4 M completely inhibited the roots' growth and gravitropic response. At a concentration of 10-6 M oryzalin, root growth was inhibited by 47% at 8 hr when compared to control. Gravitropic response was inhibited by about 38% compared to control in roots treated with 10-6 M oryzalin for 4 hr. To understand the role of oryzalin in the regulation of root growth and gravitropic response, we measured ethylene production in root segments treated with oryzalin. It was found that the addition of oryzalin stimulated ethylene production through the activation of ACC oxidase and ACC synthase genes, which are key components in the synthesis of ethylene. From these findings, it can be inferred that oryzalin inhibits the growth and gravitropic response of Arabidopsis roots by stimulating ethylene production. The increased ethylene alters the arrangement of the microtubules, which eventually interferes with the growth of the cell wall.

Allelopathic Effects of Medicinal Plants on Echinoclhoa crus-galli Beauv. (약용작물의 Allelopathy 효과에 관한 연구)

  • Kim, Sang-Yeol;Kim, Chil-Ryong;Park, Sung-Tae;Kim, Soon-Chul
    • Korean Journal of Weed Science
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    • v.17 no.4
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    • pp.407-412
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    • 1997
  • Allelopathic activity of methanol extract from medicinal plants was determined based on the inhibition of germination, shoot and root length of Echinochloa crus-galli. The response was depended on the source of extracts and concentrations. Of the 20 test plants tested, Cnidium officinale showed most inhibition according to germination, shoot and root length of E. crus-galli. Based on the plant parts, the root extract of C. officinale had more inhibitory effect on E. crus-galli than the stem+leaf extract. Phytotoxic effects of the root extract on E. crus-galli showed 75% inhibition of seed germination, 95% inhibition of shoot growth, and 100% inhibition of root growth at the concentration of 1g/petridish. Sequential partitioning of root extract with organic solvents with increasing polarity showed that the ethylacetate fraction had the greatest inhibitory effect on E. crus-galli. Germination was not inhibited at any rate of ethylacetate fraction but shoot and root growth, were significantly inhibited even at 2mg/petridish. This indicates that the most toxic phytotoxin present in the root extract may be moderate polar compound.

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Characteristics of Germination and Early Growth of Parasenecio firmus in Container by Shading Treatment (차광처리에 따른 병풍쌈의 용기 내 발아 및 초기생장 특성)

  • Song, Ki Seon;Jeon, Kwon Seok;Kim, Chang Hwan;Yoon, Jun Hyuck;Park, Yong Bae;Kim, Jong Jin
    • Journal of Korean Society of Forest Science
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    • v.103 no.4
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    • pp.547-555
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    • 2014
  • This study was carried out in order to investigate the germination and the early growth characteristics of Parasenecio firmus. Seed pre-treatment before the each experiment was carried out by shading treatment (with drying at room temperature (DRT), drying at low temperature (DLT) and water soaking (WS) for 48 hours). Experiment was performed by shading treatment (full sunlight, 35%, 50%, 75%, and 95% shading). Seeds of Parasenecio firmus were surveyed the highest germination rate (61.1%) in full sunlight with WS (overall 25.7~61.1%). Height was surveyed the highest under 95% shading. And root collar diameter was surveyed the highest in full sunlight. Fresh weights (leaf, shoot, root and total) were the highest under 50% shading. Dry weights (leaf, shoot and total) were the highest under 75% shading. It was indicated the lowest leaf, shoot, root and total under 95% shading. Leaf growth (leaf area, leaf length, leaf width and leaf thickness) and root growth (total root length, root project area, root surface area, root diameter and root volume) were good under 35%~75% shading, but the lowest under 95% shading. As a result of surveying the whole experiment, seed of Parasenecio firmus grows well under 50%~75% shading after germination by high sunlight with water soaking (WS).

The Optimal Culture Conditions and Antifungal Activity of Culture Extract from Oudemansiella mucida (끈적긴뿌리버섯(Oudemansiella mucida)의 최적배양조건 및 배양 추출액의 항균작용에 관한 연구)

  • Choi, Mi-Ryue;Cho, Hae-Jin;Lee, Jae-Seong;Kim, Hye-Young;Lee, Tae-Soo
    • The Korean Journal of Mycology
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    • v.39 no.2
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    • pp.91-98
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    • 2011
  • Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to produce antifungal substances to inhibit the mycelial growth and spore germination of the plant pathogenic fungi. To produce good amount of antifungal substances from culture media, the optimal culture conditions of O. mucida were investigated. The most favorable conditions for the mycelial growth were $25^{\circ}C$ and pH 5 in potato dextrose agar. The most favorable carbon and nitrogen sources promoting mycelial growth were maltose and calcium nitrate, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplemented to the basal medium as a carbon source. The optimal mycelial growth of O. mucida was found in the Hennerberg medium. The crude extract from submerged culture of potato dextrose broth exhibited inhibition of mycelial growth of Colletotrichum acutatum, Botrytis cinerea and Pyricularia oryzae but, fungicidal activity is not good enough to compared with commercially available fungicides tested. Therefore, the antifungal substances extracted from submerged culture of O. mucida might have a potential to be used for biocontrol agent of fungal diseases of plants.

The effect of extracts of industrial plant resources on germination and growth of Raphanus sativus L. (주요 特用資源植物 抽出物이 무 發芽 및 초기 生長抑制에 미치는 영향)

  • 이현모;임정대;김명조;유창연
    • Korean Journal of Plant Resources
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    • v.14 no.2
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    • pp.108-115
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    • 2001
  • For determining the effect of extracts exudated from ten industrial plant resources on germination and early growth of shoot and root of radish the allelopathic compounds screened. Germination rate and seedling growth of radish differed depending on plant species and extract concentration. Lower levels of 1% extracts exudated from industrial plant resources promoted the rate of germination of radish while higher levels of 10% extract inhibited the rate of germination. Germination rate, shoot growth and root growth of radish in higher concentration of extract also differed depending on the plant species exudated and extract concentraton of industrial plant resources. Higher concentration of extracts from Xanthium strutnarium L. Angellica gigas Nakai, and Solanum nigrum L. were completely inhibited the germination while those from Cichorium intybus L., and Polygonatum officinale Allioni were not inhibited. Extracts exudated from Xanthium strutnarium L, Angellica gigas Nakai, and Solanum nigrum L. showed the strong inhibition of shoot and root growth of radish while those from Cichorium intybus L., and Polygonatum officinale Allioni did not show the bioactivity in shoot and root growth of radish..

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