• Korean journal of applied entomology
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• v.45 no.1 s.142
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• pp.79-85
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• 2006

This study was conducted to get basic information on the occurrence of plant-parasitic nematodes for the establishment of nematode management strategy in major potato production areas in Korea. Nationwide soil collection was done in 11 areas of Cheju, Yesan, Gimchun, Goryoung, Hong chun, Pyungchang, Gimjae, Milyang, Namwon, Gangnung, and Inje in 2004-2005. Root-hot nematode juveniles(J2) were detected in 30 samples among the 50 samples. The average density was 12-69 J2/100cc soil. Pratylenchus sp., Helicotylenchus sp., Ditylenchus sp., Tylenchus sp., and Tylenchorhynchus sp. were also detected in various locations, however, their densities were very low. Root-knot nematode females were collected from tomato roots inoculated with the potato field soils for PCR-RFLP identification. The females from Cheju, Milyang, and Goryung showed PCR products of 500 bp. And the Dra I restriction enzyme digestions showing 290 bp and 230 bp fragments confirmed their identity as Meloidogyne hapla.

• Korean journal of applied entomology
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• v.31 no.4
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• pp.396-415
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• 1992

To investigate identification of species distribution state, population density of plant parasitic nematodes and damaged state by parasitism of nematodes with soil samples taken from major cultivating area of medicinal herbs in Kyungbuk districts are summarized as follow; total species belonging to genera in families were identified from 43 different medicinal herbs. The dominant nematode species on medicinal herbs was Meloidogyne hapla by 55.6% of field infection. Damaged state by parasitizm of Root-knot nematode, M. hapla, M. incognita are severed 15 medical plant except P. japonica, infected field were in 54.5~88.0%, and highered in population densities of 2nd larvae, Gall, Egg sac. Percentage of yield-decrease were 57.8% in A. gigas, 49.1% in P.japonica. Lighten effect of M. spp. by cropping system were lowered in population density in Rice+Paeony than Paeony+Paeony in paddy field, and also lowered in Sesame+Paeony or one year fallowing after harvest Paeony than Red pepper+Pseony, Paeony+Paeony in upland field.

• The Korean Journal of Pesticide Science
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• v.8 no.4
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• pp.353-357
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• 2004

Large economic losses have been reported by root-knot nematide, Meloidogyne incognita, in Korea. However, fewy environmentally-friendly nematicide alternatives for the control of M. incognita have been developed. This study was conducted of Korean native plants to screen nematicidal activity which could be used by organic farmers. Methanol extracts (1,000 mg $L^{-1}$) from fifty-three Korean native plants were applied to 24-well tissue culture testplates containing $1\times10^2$ M incognita and the nematicidal activities were determined. Nematicidal activities of the methanol extracts in common purslain (Portulaca oleraceae L.) and water mouse-ear-chickweed (Stellaria aquatica Scop.) were 60.0% and 40.6%, respectively, compared with that of control, however, those of other forty-one plants were less than 30%. Our results suggest that the methanol extracts of common purslain and water mouse-ear-chickweed contain nematicidal active compounds.

• The Korean Journal of Pesticide Science
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• v.9 no.3
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• pp.268-273
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• 2005

The cultivating agroproducts are damaged by the Meloidogyne spp. which are gradually increasing in farm land soil. No effective control method for Meloidogyne spp., however, is available. Pasteuria penetrans which is one of the microorganisms in soil is used for biological control of Meloidogyne spp. although the method of mass production is limited. This study was conducted to investigate attachment and mass production effect of P. penetrans to M. arenaria under different temperatures (10, 30, 50 and $70^{\circ}C$) and pH values (4, 7 and 10). Attachment rates under these temperature and pH were more than 96% and 80%, respectively. In mass production rates, the number of P. penetrans attached on M. arenaria under different temperatures and pH were highly increased in root of tomato but not significantly different. Therefore, we concluded that P. penetrans can survive and attach on M. arenaria under various conditions. This method for mass production of P. penetrans can be provided to develop environmentally-friendly nematicide.

• Journal of Ginseng Research
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• v.20 no.3
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• pp.331-338
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• 1996

This study was conducted to screen the antagonistic plants on northern root-knot nematode (Meloidogyne) and to utilize those in its control. Egg hatching of M. hapla was found to be inhibited by 17 plant methanol extracts, and 11 plant extracts among them were also toxic to M. hapla second stage juvenile. Egg hatching of iW. hapla was also found to be inhibited by squeezed extracts of Cassia tora and Zea mays, and they were also toxic to M. hapla second stage juvenile. Extracts of Achyranthes japonica, Melia axedrach and Acorus graminens were toxic to M. hapla second stage with a juvenile mortality above 70clc at the 10 folds diluted concent ration and A. graminens was toxic to tested juvenile mortality above 50% at the 100 folds diluted concentration. The toxicity was directly propotional to the diluted concentration of the plant extracts and to the exposure period. Punica granatum, Acorns graminens and Melia axedrach were effective in inhibiting root penetration of JW. hapla juveniles, among of them p. granatum is most effective Percent inhibition of penetration by second and third stage juveniles into tomato slants penetrating by it was 72.7 and 82.4%, respectively.