• Title/Summary/Keyword: 뿌리생장

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Comparison of Some Propagation Methods in Paeonia albiflora Pallas and Effective Components of Paeonia albiflora Pallas Root Grafted with Paeonia moutan Sims (작약의 번식방법과 작약 / 목단 접목근의 유효성분 비교)

  • 이희덕
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.3
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    • pp.283-287
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    • 1992
  • This experiment was carried in order to compare some propagation methods and the effective components in the root of Paeonia albiflora pallas, Paeonia moutan Sims and Paeonia albiflora pallas root grafted with Paeonia mouton sims The optimum seeding time of August 20 to september 10 for increasing germination ratio. The growing and developing of Paeonia albiflora Pall. for root dividing methods was advantageous to practice at Autum than at spring season. The shoot cutting method during the flowering time was proper to increase the rooting ratio with the aid of Ruton, the plant growth regulator but not emerged of apical bud. The grafting stages between the Paeonia albiflora pall. and Paeonia moutan Sims was available to practice during the September for increasing the thickness growth of root. Among the effective components of the paeony the paeoniflorin was shown high, while paeonol in paeony moutan. The paeoniflorin of herbaceous paeony / paeony moutan was increased about 75.9% than herbaceous paeony alone, and the other compoents are similar to those of herbaceous paeony root.

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Study on the Microbial Degradation of Nicotine (1) -Isolation and Identification of Nicotinophiles (미생물에 의한 니코틴 분해 연구(1) -니코틴 분해세균의 분리 및 동정)

  • 강은희;윤경하
    • Journal of the Korean Society of Tobacco Science
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    • v.2 no.1
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    • pp.17-27
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    • 1980
  • From 143 sources of collected samples, the distribution of nicotinophiles were investigated and the biological characteristics as well as the rate of nicotine degradation were determined for the selected 34 strains which could grow successfully in the nicotine media, and one of the most effective strains was chosen and identified at the species level. Nicotinophils were distributed abundantly in the soils rich with organic materials, tobacco seed and root. The selected 34 strains were classified into 7 genus and identified with 4 strains of Arthrobacter, 11 strains of non-pigmented Pseudomonas, 2 strains of pigmented Pseudomonas, 6 strains of Alkaligenes, 5 strains Chromobacter 2 strains of Listeria and 4 strains of Achromobacter. Pseeudomonas and Alkaligenes were better than other genus in the rate of nicotine degradation and tobacco seed and root were also good sources for the isolation of effective nicotinophiles. Amnog 34 strains, strain NCT 27 which exhibited 97.l% of nicotine degradation rate was the best one for nicotine degradation and was indentified with Pseudomonas putida.

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Fertility Prolongation of the Solid Typed Organic Fertilizer from Cattle Manure (축분 유기질비료의 고형화에 의한 비효 연장)

  • Joo Young-Kyoo;Jung Yeong-Sang;Lee Sang-Kook;Kim Eun-Kyu
    • Asian Journal of Turfgrass Science
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    • v.18 no.2
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    • pp.71-76
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    • 2004
  • Solid typed organic fertilizer from cattle manure may Increase plant root growth with the residual effect on soil fertility if it was applied directly into root system. It may also increase labour efficiency by simplifying the work process of manure application to soil. This research was carried out from May to October 1999 to study the fertility prolongation effects of bar typed organic fertilizer from poultry manure by analysing plant growth model compare with those of the chemical fertilizer or powder typed manure. The results showed that the bar typed organic fertilizer increased growth rates of shoot and root system with extending the effect of its fertility by slow releasing on pepper plant. Especially, solidifying organic manure into the bar type made possible the application of cattle manure under plastic mulching. And it also has benefits on simplifying the application process of the fertilization and effect on an extending soil fertility.

Correlation between In vitro Plant Regeneration and Polarity with Boxthorn (Lycium chinense Mill.) Seedlings (구기자나무 유식물체를 이용한 식물체의 재분화와 극성과의 관계)

  • Kwon Hye-Kyoung;Cho Yi-Yun;Yoon Eui-Soo
    • Korean Journal of Plant Resources
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    • v.19 no.2
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    • pp.308-314
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    • 2006
  • This experiment was conducted out to investigate the effect of plant growth regulators on callus and shoot formation. The callus formation was effective on 1/2 MS medium containing 2,4-D, while shoot formation was suppressed. Shoot formation and differentiation were the highest in combination 0.1 mg/L of IAA and 0.1 mg/L of BA. The polarity of explants was investigated from cotyledon, which excised 20% of each basal and terminal parts. Formation of shoot was induced from excised ends of the basal part. In excised ends of the basal part, callus was induced vigorously and shoots were produced lately. Root induction was easily achieved in 1/3 MS medium from the adventitious shoot and more than 90% of regenerated plantlets acclimatized successfully and flowered normally.

Plant Regeneration from Protoplasts of Suspension Cultured Cells in Arabidopsis thaliana (애기장대(Arabidopsis thaliana) 현탁배양세포의 원형질체로부터 식물체 재분화)

  • 김명덕;김준철;진창덕;임창진;한태진
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.2
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    • pp.125-131
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    • 2000
  • Protoplasts of Arabidopsis thaliana were easily isolated from the shoot-forming (SF) suspension-cultured cell clusters with 4 hours-shaking condition (40 rpm) on CPD enzyme solution containing 1% cellulase R-10, 0.25% pectolyase Y-23 and 0.5% driselase. Protoplasts were cultured on liquid KAO medium supplemented with 1 mg/L 2,4-D, 0.5 mg/L kinetin, 200 mg/L spermidine and 68 g/L glucose. Also, protoplasts were cultured on 0.2 $\mu$M membrane filter placed onto CP solid medium containing the suspension cells as feeder cells in the dark at $25^{\circ}C$ for 4 weeks. Protoplast-derived-SF calli were cultured on MS medium containing 0.05 mg/L IAA, 7 mg/L 2 ip and 30 g/L sucrose under the continuous illumination for four weeks. The frequency of shoot formation was about 60%. The regenerants were transferred into potting soil to grow mature plants. The regenerants formed the silques with seeds after 8 weeks of cultures.

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A Potential Biological Weed Control by Golden Apple Snail(Pomacea canaliculata) in Wet Hill Seeded Rice with Iron-coated Seeds (벼 무논점파(철분코팅종자)재배에서 친환경 잡초관리를 위한 왕우렁이 이용 기술개발)

  • Park, Kwang-Ho
    • Korean Journal of Weed Science
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    • v.32 no.3
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    • pp.273-279
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    • 2012
  • This experiment was conducted to determine a potential effective biological weed control and/or rice young seedling injury by golden apple snail (Pomacea canaliculata) at wet hill seeded rice field. The rice seeds used were treated by iron-coating. The efficacy of weed control as affected by golden apple snail has been tested with twice applications of young golden apple snails of 12 kg (24,000 young snails, $0.5g{\pm}10%$ per young snail, 30~40 days after hatching) per ha at the same day after harrowing and applied with rate of 10 kg (20,000 young snails) per ha at 15days after seeding, respectively. The comparison of this experiment was of the conventional machine transplanted rice paddy field in terms of weed control and rice plant injury as a visual grade. The weed efficacy was of 100% similar with the conventional paddy field which was applied by systematic herbicides of pre-emergence and post-emergence chemical herbicides and there was 1~3% significant rice young seedling injury but no yield losses and grain quality due to the input of golden apple snail.

Evaluation of Safety and Fertilizer Effect of Composts According to Rapid Fermentation Composting of Agro·Livestock·Marine Organic Waste (농·축·수산 폐기물의 고속 발효 퇴비화 시료의 안정성 및 비효 평가)

  • Chang, Ki-Woon;Lee, Jong-Jin;Lee, Jong-Eun;Yu, Yeong-Seok
    • Journal of the Korea Organic Resources Recycling Association
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    • v.10 no.3
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    • pp.85-91
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    • 2002
  • To investigate the agricultural material application possibility of the compost made by the fermenting tank. We evaluate the stability fertility of the compost In evaluation of the stability of the compost, In germination index of test, germination test of lettuce of little resistibility not germinated wholly, in the radish of resistibility, both Shinco and TM-101 is showed 61%, 55% of germination rate, length of root not showed wholly. In stability fertility of the compost, early growth of radish in a little treatment applied was superior to other treatments except for TM-101 treatment, but the latter growth of those was showed opposite result. In evaluation of the fertility of compost, Primary growth of lettuce was superior in treatment applied 1.5t/10a, the latter growth of those was superior in treatment applied 3t/10a.

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Fungal Flora of Ullung Island (IV) -on some agaric fungi- (울릉도의 균류상 (IV) -수종 주름버섯류에 대하여-)

  • Jung, Hack-Sung
    • The Korean Journal of Mycology
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    • v.21 no.1
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    • pp.64-72
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    • 1993
  • Fresh fungi were collected during five field trips to Ullung Island from October of 1989 to September of 1991. Through the observation of some agaric fungi, thirty mushrooms were identified to the species and are listed as below. Among them, three species, Crepidotus geophilus, Mycena elegans, ana Mycena carolinensis were confirmed new to Korea and are registered here with descriptions.

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Control Effects of 3-(4-Hydroxyphenyl)-propionic Acid Isolated Xenorhabdus nematophila K1 against Phytophthora Blight and Anthracnose of Red Pepper (Xenorhabdus nematophila K1 대사물질 3-(4-hydroxyphenyl)-propionic acid의 고추 역병과 탄저병에 대한 방제 효과)

  • Cheon, Wonsu;Kim, Doyeon;Kim, Yonggyun;Hong, Yong Pyo;Yi, Youngkeun
    • Research in Plant Disease
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    • v.19 no.3
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    • pp.208-215
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    • 2013
  • 3-(4-Hydroxyphenyl)-propionic acid (HPP) is a bacterial metabolite synthesized and released by an entomopathogenic bacterium Xenorhabdus nematophila K1. In this study, the control efficacy of HPP was tested against Phytophthora blight and anthracnose of red pepper plants. HPP suppressed mycelial growth of Phytophthora blight and anthracnose pathogens. Under natural sunlight condition, HPP maintained the antifungal activity on the diseases for at least twenty five days. The antifungal activity was not decreased even in the condition of soil-water. It was proved that HPP was able to penetrate the roots and travel upward of the red pepper plants. When HPP suspension was applied to soil rhizosphere before transplanting the red pepper seedlings or was regularly sprayed to the foliage of the plants with ten days interval, it resulted in significant reduction of the disease occurrences (Phytophthora blight and anthracnose) without any phytotoxicity. These results suggested that HPP can be developed to a systemic agrochemical against Phytophthora blight and anthracnose of red pepper plants.

Changes of RNA and Protein During Callus Induction and Plant Regeneration from Perilla frutescens (들깨로부터 Callus의 유기와 재분화에 따른 단백질 및 RNA의 변화)

  • 정상훈;양선경;김현경;정대수;조영수;김도훈
    • Journal of Life Science
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    • v.9 no.1
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    • pp.29-34
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    • 1999
  • Cotyledon and hypocotyl explants of perilla were cultured on MS medium containing a combined concentration of BA(0.5, 1.0 and 1.5mg/$\ell$) and NAA(0.1, 0.5 and 2.0mg/$\ell$) in order to regenerate the explant and induce the callus. The best regeneration of the explant and induction of the callus were observed in a combined concenteration of 0.5mg/$\ell$ of BA and 0.5mg/$\ell$ NAA both in cotyledon and hypocotyl explants. In cotyledon explants, rooting was achieved upon transferring shoots to MS medium containing 0.5mg/$\ell$ of BA and 0.1mg/$\ell$ of NAA. We also investigated the change of protein and RNA content on developmental stage of callus and plant regeneration of perilla. Protein content was increased but RNA content was decreased as the culture period increases. The banding pattern of polypeptide revealed that both 30KD and 45KD polypeptides were obvious in cotyledon obtained from pre-culture explants, but only 30KD polypeptide was further getting obvious as the culture period increases.

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