• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.99-99
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• 1995

In vivo 실험결과, FVA가 감염된 BALB/c mice 에게 AZT를 18일간 경구투여시 (100mg/kg/day) 대조군에 비해 비장의 비대를 82% 억제하였으며, 이들에게 FVA를 재감염시킨 후 18일 뒤에도 비장의 비대가 63% 억제되었다. 그러나 혈액중 빈혈의 지표는 정상으로 회복하지 못하였다. 한편, tannic acid 는 (500mg/kg/day) 비장 비대를 55% 억제하였으며, 빈혈지표도 정상으로 회복되었다. 그러나, FVA를 재감염시킨 경우에는 비장의 비대가 억제되지 않았다. 반면에, tannic acid 와 AZT 를 병용투여시 비장의 비대를 98% 억제하여 상승효과를 초래하였으며, 빈혈지표도 정상으로 회복되어 독성을 감소시켰다. 또한 이들에게 FVA를 재감염시킨 경우에도 비장의 비대가 63% 억제되었다. 한편, In vitro 실험결과 AZT 의 경우 $IC_{50}$/이 0,01$\mu$M인 반면에 tannic acid의 경우에는 5 $\mu$M로서 약 1/500 의 효력을 나타내었다. 이상의 결과는 tannic acid 는 AZT 와는 다른 기전에 의해 FVA 의 감염에 의한 비장비대 및 빈혈을 억제하는 것으로 고려된다.

• Proceedings of the Korean Information Science Society Conference
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• 2005.07b
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• pp.874-876
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• 2005

간경변을 보유한 환자의 복부 CT 영상을 관찰한 결과 비장의 웨이브 패턴(wave pattern)이 정상간을 보유한 사람의 복부 CT 영상과 차이가 있음이 발견되었다. 한편 간경변에 동반된 비장은 일반적으로 비대한 것으로 알려져 있다. 따라서 본 논문에서는 이러한 두가지 원리를 바탕으로, 복부 CT 영상에서 비장의 웨이브 패턴을 이용하여 비장 비대를 효과적으로 진단하는 새로운 방법을 제시한다. 제안된 방법에서는 단순히 비장의 크기만물 이용하지 않고 비장의 형태 변화를 바탕으로 국소적 비장 비대의 자동 판정을 효과적으로 수행할 수 있음을 입증하였다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.79-79
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• 1993

BALB/C mice 에게 FVA를 감염시킨 후, zidobudine을 1 mg/ml 의 농도로 식수에 용해하여 18일 동안 자유롭게 섭취 시켰을때 (150-200 mg/kg/day) 비장비대가 90% 억제되었고, 혈액 중 reverse transcriptase 활성은 85%로 억제효과를 나타내었으며, 빈혈지수는 회복되는 경향을 나타내었다. 한편, ddI 는 AZT와 동일 용량에서 비장비대는 17%, 혈액 중 reverse transcriptase 억제효과는 34% 억제효과를 나타내었다. 반면에 ddC는 단독투여시 비장비대를 38% 억제하였으며, interferon $\alpha$와 병용투여시 83%의 억제효과를 나타내었다. 이상은 FVA 감염에 의한 mice의 비장비대를 지표로 하여 reverse transcriptase의 억제효과를 지닌 항 AIDS약물의 약효검색법으로 활용할 수 있음을 제시해준다.

• The KIPS Transactions:PartB
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• v.12B no.5 s.101
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• pp.553-560
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• 2005

Generally, it is known that the spleen accompanied by liver cirrhosis is hypertrophied or enlarged. We examined the wave pattern of the spleen by using abdominal CT images of a patient with liver cirrhosis, and found that they are different from those of a person with a normal liver In the abdominal CT image of the patient with liver cirrhosis, there is a deep wave part on the left side of the spleen. In the case of the normal liver, there are waves on the left side, but they aren't deep. Therefore, the total area of waving parts of the spleen with liver cirrhosis is found to be greater than that of the spleen with the normal liver. Moreover, when examining circularity by abstracting the waves of the spleen from the image iO liver cirrhosis, we found they are more circular than those of the spleen accompanied by a normal liver. This paper suggests an automatic method to diagnose splenic enlargement by using the wave pattern of the spleen in abdominal CT images on the basis of the two principles. It tells us that we can judge if the abdomen has a focal splenic enlargement automatically, without the manual test of the size of spleen, only with the shape of spleen.

• Journal of Gastric Cancer
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• v.9 no.2
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• pp.63-67
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• 2009

Splenic marginal zone lymphoma (SMZL) is a rare type of non-Hodgkin's lymphoma (NHL). We report here on a patient who displayed the synchronous occurrence of SMZL and early gastric cancer (EGC). The patient was a 74 year-old male with liver cirrhosis. An EGC in the gastric antrum was diagnosed and the preoperative abdomen computed tomography scan revealed splenomegaly and intra-abdominal lymphadenopathy. We performed subtotal gastrectomy and the postoperative pathologic examination revealed adenocarcinoma limited to the gastric mucosa and SMZL in the lymph nodes. The patient recovered from the surgery without complications and is now awaiting chemotherapy. SMZL has an indolent clinical course with good long-term survival and so there is the possibility of the occurrence of a second primary malignancy. Rare cases of a second primary malignancy being diagnosed along with SMZL have been described in the literature. Patients with SMZL should be carefully followed after treatment to detect the possible occurance of a second primary malignancy.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.278-278
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• 1994

HIV와 유사하게 retro virus의 일종인 Friend virus of Anemia strain (FVA)을 BALB/c mice 에게 감염시 reverse transcriptase 의 활성에 의해 비장의 erythroid progenitor cell 에서 증식되므로서 비장의 비대 및 빈혈을 초래하게 된다. 이를 지표로 하여 reverse transcriptase 억제작용을 지닌 항 virus 약물을 천연성분으로 부터 검색하고자 하였다. 우선, 대조약물로 사용한 기존의 항 AIDS 약물인 zidovudine (AZT) 을 FVA 가 감염된 BAB/c mice 에게 18일간 투여시 (약 100 mg/kg/day, p.o. ) 대조군에 비해 비장의 비대 및 reverse transcriptase 활성이 90% 이상 억제되었으며, 이들의 혈청을 정상 BALB/c mice 에게 재투여시에도 유사한 결과를 나타내어 reverse transcriptase 를 억제하므로서 항 virus 작용을 나타낸다는 것을 입증하였다. 그러나, 혈액중 Hemoglobin등 빈혈의 지수는 대조군과 유사한 수치를 나타내므로서 정상으로 회복되지를 못하였다. 이것은 zidovudine 자체가 지니는 골수억제에 의한 부작용 때문인 것으로 고려된다.

• Journal of the Korean Society of Radiology
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• v.15 no.6
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• pp.883-889
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• 2021

This study was conducted on 101 patients who visited hospital for abdominal ultrasonography from May 2020 to December 2020. The purpose of this study was to find out the elasticity according to the ultrasound images (echo pattern, splenomegaly, hepatitis) during the ultrasound examination using the shear wave elastography. The shear wave elastography value of the normal group of the echo pattern was 5.75±1.58 kPa, and the group with the abnormal echo pattern was 8.84±4.94 kPa, and the shear wave elastography value of the abnormal group was high (p<0.05). In normal spleen size, hepatic elasticity value was 6.33±2.54 kPa, and hepatic elasticity value of splenomegaly was 13.73±5.48 kPa. In the case of splenomegaly, the liver elasticity value was high, and there was a statistically significant difference (p<0.05). As the spleen size increased, the liver elasticity value increased by 1.485 times, and as hepatitis progressed, the liver elasticity value increased by 1.573 times (p<0.05). As a result of analysis of concordance between ultrasound imaging findings and shear wave elastography, the Kappa value was found to be as high as 0.922 (p<0.05), which showed high concordance between the two test methods. Additional comparisons of liver elasticity values in shearwave elastography tests along with liver ultrasound findings are thought to be of great help in diagnosing liver fibrosis.

• Journal of fish pathology
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• v.13 no.2
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• pp.121-127
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• 2000

From August to October 1998, over 60% mortality of cultured striped beakperch Oplegnathus fasciatus was occurred in net cages along the southern coast of Korea. Moribund fish showed some clinical signs of lethargic behavior, dark coloration or decoloration, severe gill anemia and enlargement of spleen. Also enlarged basophilic cells showing Feulgen -positive reaction were observed in the tissue section of spleen, kidney, liver and heart of the diseased fish. GF cells inoculated with spleen homogenate of diseased fish produced cytopathic effect of enlarged and rounded cells, therefore the causative virus was isolated from diseased fish. Striped beakperch fingerlings intraperitoneally inoculated with the causative virus ($10^4TCID_{50}$/0.1 ml) revealed symptoms similar to those of naturally infected fish and died from 7 to 14 days post injection. Transmission electron microscopy revealed that the causative virus was enveloped icosahedral particle with 120~130 nm in diameter. PCR products of the expected size (500 bp) were amplified with a primer set based on the ATPase gene of RSIV(red sea bream iridovirus) using template DNAs which were extracted from the spleen of diseased fish and GF cells inoculated with the causative virus. According to the analysis of nucleotide sequence of these PCR products, the sequence from ATPase cDNA gene of the causative virus showed 95% homology with that of RSIV. These results indicate that the mass mortality in the cultured striped beakperch was caused by the infection of iridovirus similar to RSIV.

• Journal of fish pathology
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• v.16 no.3
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• pp.153-159
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• 2003

The high mortality of cultured juvenile turbot, Scophthalmus maximus occurred in Gochang on June, 2003. The diseased fish was lethargic with reduced feed intake. Grossly, these fish showed pale body, abdominal extension and exophthalmia. The dominant internal gross features of diseased fish were severely enlarged spleen, pale gills and/ or liver. Diseased fish histologically showed basophilic enlarged cells in the kidney, spleen, gill, heart, stomach, intestine, liver, pancreas and adipose tissue. Transmission electron microscopy (TEM) reveled hexagonal virions in the cytoplasm of necrotic cells. The viral particles lead a central electron-dense core and an electron translucent zone, and were 136-159 nm in diameter. These results suggest that the virus belonging to the iridoviridae was responsible for the mortality of cultured juvenile turbot.

• The Journal of the Korean Society for Microbiology
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• v.13 no.1
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• pp.55-62
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• 1978

Adult mice were injected with a single sublethal dose of cyclophosphamide. Effects of the drug on the body weight, spleen weight, and morphology of the peripheral lymphoid system have been analysed. The body weights of the mice given cyclophosphamide(300mg/kg body weight) decreased slightly and returned to normal quickly. Spleen weights, however, changed greatly by keeping the process of decrease, recovery, overshoot, and gradual return to normal only by 20 days. Histologic examinations of spleen and popliteal lymph node showed that follicles disappeared 1 to 2 days before periarteriolar lymphatic sheath or paracortex. At the peak of splenomegaly, the architectures of spleen and lymph node were replaced with the interstitial tissue composed of dense and uniform layer of lymphoid cells. With the return of spleen weight to normal range, the architecturles returned to normal. Our results clearly indicated that cyclophosphamide affected not only B cells but also T cells. These results seemed to suggest that augmentation of delayed-type hypersensitivity by cyclophosphamide may be due to the eliminateion of the suppressor T cells.