• Title/Summary/Keyword: 비드법

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Microfilter Chip Fabrication for Bead-Based Immunoassay (비드를 이용한 면역분석용 마이크로필터 칩의 제작)

  • Lee, Seung-Woo;Ahn, Yoo-Min;Chai, Young-Gyu
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.28 no.9
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    • pp.1429-1434
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    • 2004
  • Immunoassay is one of the important analytical methods for clinical diagnoses and biochemical studies, but needs a long time, troublesome procedures and expensive reagents. In this study, therefore, we propose the micro filter chip with microbeads for immunoassay, which has pillar structures. The advantage of the proposed micro filter chip is to use simple fabrication process and cheap materials. The mold was made by the photolithography technique with Si wafer and negative photoresist SU-8. The replica was made of PDMS, bonded on the pyrex glass. The micro filter chip consists of inlet channel, filter chamber and outlet channel. HBV (Hepatitius B virus) monoclonal antibody (Ag1) labeled with biotin were immobilized onto streptavidin coated beads of 30∼50 $\mu$m size. Fluorescein isothiocyanate (FITC)-labeled HBV monoclonal antibody (Ag8) was used to detect HBsAg (Hebatitis B virus surface Antigen), and fluorescence intensity was monitored by epi-fluorescence microscope. In this study, the immune response of less than 30 min was obtained with with the use of 100 $m\ell$ of sample.

An Weldability Estimation of Laser Welded Specimens (레이저 용접물의 용접성 평가)

  • Lee, Jeong-Ick
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.16 no.1
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    • pp.60-68
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    • 2007
  • It has been conducted by laser vision sensor for weldability estimation of front-bead after doing high speed butt laser welding of any condition. It has been developed a real time GUI(Graphic User Interface) system for weldability application in the basis of texts and field qualify levels. In the reference of bead imperfections, defects absolute position and defects intensity index of front-bead in the basis of formability reference, it has been produced a weldability estimation and defects intensity index of back-bead by back propagation neural network. In the result of by comparing measuring data by laser vision sensor of back-bead and data by back propagation neural network of one, it has been shown the similar results. Finally, under knowledge of welding condition in production line, it has been conducted a weldability estimation of back-bead only in knowledge of informations of front-bead data without using laser vision sensor or welding inspection experts and furthermore it can be used data for final inspection results of back-bead.

The Development and Application of Sheet Metal Forming Technology (박판성형기술의 개발과 적용)

  • 박춘달;이장희;양동열;허훈;정동원
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1994.06a
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    • pp.147-162
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    • 1994
  • Generally, the forming process of sheet metal is very complex and difficult process because of many variables such as tool geometry, material properties and lubrication. In this view point, the numerical analysis of sheet metal forming process is very difficult. High speed computer is used to model complex sheet metal forming process on a reasonable time scale. The design and development of sheet metal parts in the automotive industry and the need for improved sheet forming process and reduced part development cost have led to the use of computer simulation in tool/die design of sheet metal pressing. HMC(Hyundai Mator Company) has invested to develop programs for analysis of sheet metal forming process with connection of Universities. As a result, several programs were developed. Recently, the commercial software, PAM-STAMP of ESI was installed and is being tried to application of it to the real automotive panels. This article reviews the ongoing activities on development and application of analytical modeling of sheet metal forming at HMC.

Changes in Insulation Performance of Organic Insulating Materials for Building Construction by Accelerated Durability Test Conditions (가속내구성 조건에 따른 건축용 유기계 단열재의 단열성능 변화)

  • Lim, Soon-Hyun;Lee, Gun-Cheol
    • Journal of the Korea Institute of Building Construction
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    • v.16 no.6
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    • pp.595-601
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    • 2016
  • The insulation performance of the insulation currently used in building structures is reflected only during design based on initial performance and the reduction in heat insulation performance due to the degradation of long-term durability is not reflected. This study reviewed the degradation of heat insulation performance due to the durability degradation of insulating materials through the accelerated durability test. The study findings showed that the foamed polystyrene insulation bead method did not show performance degradation due to aging in the standard environmental condition and laboratory accelerated test condition but the performance is degraded in the freeze-thaw test condition. On the other hand, in the case of the extrusion method, the degradation of the heat insulation performance was less in the freeze-thaw test condition, but the rapid performance degradation was caused by the release of the internal gas at the beginning of aging. In addition, the hard polyurethane foam insulation showed better initial insulation performance than other insulation materials, but the performance was found to be degraded somewhat under laboratory accelerated test conditions and freeze-thaw test conditions.

Decomposition of BTEX by Gas-phase Photo Reactor (기상광반응기를 이용한 BTEX의 분해)

  • Lee, Nam-Hui;Lee, Gang;Hwang, Du-Seon;Kim, Se-Gi;Kim, Seon-Jae
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2003.03a
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    • pp.77-77
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    • 2003
  • 산업화에 따라 각종 산업분야에서 유기 용제의 사용이 증가하게 되었고 대기오염, 수질오염 등의 상태가 심각해져가고 있는 실정이다 그러나 다양해진 오염물질을 처리하는 데 있어서 기존의 산화처리방법은 한계에 달하였고 새로운 처리공정으로 최근 고급산화법(AOP : Advanced Oxidation Process)에 대한 연구가 활발하게 진행되고 있다. 이에 본 연구에서는 대기환경 및 인체에 심각한 영향을 주는 VOCs(Voletail Organic compounds)중 BTEX(Benzene, Toluene, Ethylbenzene, Xylene)를 다양한 형태의 광촉매제를 이용하여 기상 광분해한 후 이에 대한 최적의 분해 조건과 분해율을 비교하고자 하였다. 순환식의 자체 제작한 기상광반응장치를 이용하여, CVD법으로 TiO$_2$ 입자를 알루미나 비드위에 코팅한 볼과 같은 방법으로 유리기판위에 코팅한 판상의 광촉매제 그리고 결정성이 각기 다른 루틸상과 아나타제 그리고 아나타제/루틸 혼합상의 TiO$_2$ 분말을 직접 이용하여 VOCs 농도와 종류, 광원의 종류 및 세기를 변화시켜 PID(Photo ionization Detector)방식의 순환식 VOCs 측정기를 이용하여 광분해율을 실시간으로 측정하였다.

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A Study on Performance Improvement of TDMA Noise in a GSM Mobile Phone using the Noise Measure Method (잡음 대책법을 이용한 GSM 방식 이동전화기의 TDMA 잡음 개선에 관한 연구)

  • Oh, Se-Kab;Choi, Jae-Myeong;Kang, Heau-Jo
    • Journal of Advanced Navigation Technology
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    • v.13 no.3
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    • pp.406-411
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    • 2009
  • In this paper, we study the method of noise analysis for analysing TDMA noise in a GSM mobile phone and describe the cause of TDMA noise. And we reduced noise by 9dB, 3.5dB, 6dB, using MIC shielding, equipped bead or capacitance, connected PCB ground based on the difference analysis method, which is one of common noise analysis methods.

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A Study on the Comparison of Chloride Ion Quantification Methods for Magnesium-Aluminum (Mg-Al) Alloy Powder (마그네슘-알루미늄(Mg-Al) 합금 분말의 염소이온 정량법의 비교에 관한 연구)

  • Yunhwan, Kim;Youngson Choe
    • Applied Chemistry for Engineering
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    • v.34 no.4
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    • pp.450-454
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    • 2023
  • Chloride ions in the alloy powder used as flux in Flux Cored Arc Welding (FCAW) can cause pores on the bead surface of the welding metal to cause defects, or chloride remaining in the alloy powder can cause corrosion of the metal. Combustion-ion chromatography is mainly used to quantify the chloride ions in alloy powder, but there is a limitation in that the equipment is expensive and requires a high degree of expertise. Therefore, this study aims to find an easy and accurate quantification method in the field by comparing combustion-ion chromatography (C-IC), which is mainly used for chloride ion quantification of alloy powder, X-ray fluorescence analysis (XRF), and potentiometric titration. In this article, magnesium-aluminum alloy powder is applied to the quantification of chloride ions because it is most commonly used as flux. This study confirmed that potentiometric titration can be applied to the quantification of chloride ions in the alloy powder in the industry field.

Preparation of Monodispersed Crosslinked Polymer Beads (단분산상으로 가교된 고분자 비드의 합성)

  • 심상은;변재만;전종원;차윤종;최순자
    • Polymer(Korea)
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    • v.24 no.3
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    • pp.287-298
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    • 2000
  • In preparing micron-sized monodisperse polystyrene beads by dispersion polymerization, the conversion, and the particle size and its distribution were affected by the reaction temperature, concentration of the monomer, solvent and initiator, molecular weight and concentration of the steric stabilizer, amount of oxygen existing in the reactor, and an appropriate combination of these starting materials. Ethanol as a dispersing agent, styrene as a monomer, PVP as a steric stabilizer, AIBN as an initiator, DVB as a cross-linking agent and toluene as a co-solvent were the basic materials for the synthesis. The reaction rate and the conversion were increased with the reaction temperature and the amount of DVB from 1 to 4%, and the conversion was saturated after 10 hours of the reaction time. The optimum reaction recipe for the preparation of the monodisperse PS beads was 25% styrene monomer, 0.5% DVB, 25% toluene, 10-15% PVP, and 2 and 4% AIBN, thereby, 3.9~4 ${\mu}{\textrm}{m}$ and 3.4~9.3 ${\mu}{\textrm}{m}$ of polystyrene beads, respectively, were successfully synthesized.

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Hydrogenation of Polycyclic Aromatic Hydrocarbons Over Pt/Kieselguhr Catalysts in a Trickle Bed Reactor (Trickle Bed Reactor에서 Pt/Kieselguhr 촉매를 이용한 다환방향족 탄화수소 수소화 반응)

  • Seung Kyo, Oh;Seohyeon, Oh;Gi Bo, Han;Byunghun, Jeong;Jong-Ki, Jeon
    • Clean Technology
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    • v.28 no.4
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    • pp.331-338
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    • 2022
  • The objective of this study is to prepare bead-type and pellet-type Pt (1 wt%)/Kieselguhr catalysts as hydrogenation catalysts for the polycyclic aromatic hydrocarbons (PAHs) included in pyrolysis fuel oil (PFO). The optimal reaction temperature to maximize the yield of saturated cyclic hydrocarbons during the PFO-cut hydrogenation reaction in a trickle bed reactor was determined to be 250 ℃. A hydrogen/PFO-cut flow rate ratio of 1800 was found to maximize 1-ring saturated cyclic compounds. The yield of saturated cyclic compound increased as the space velocity (LHSV) of PFO-cut decreased. The difference in hydrogenation reaction performance between the pellet catalyst and the bead catalyst was negligible. However, the catalyst impregnated by Pt after molding the Kieselguhr support (AI catalyst) showed higher hydrogenation activity than the catalyst molded after Pt impregnation on the Kieselguhr powder (BI catalyst), which was a common phenomenon in both the pellet catalysts and bead catalysts. This may be due to a higher number of active sites over the AI catalyst compared to the BI catalyst. It was confirmed that the pellet catalyst prepared by the AI method had the best reaction activity of the prepared catalysts in this study. The majority of the PFO-cut hydrogenation products were cyclic hydrocarbons ranging from C8 to C15, and C11 cyclic hydrocarbons had the highest distribution. It was confirmed that both a cracking reaction and hydrogenation occurred, which shifted the carbon number distribution towards light hydrocarbons.

Application of the Nonionic Surfactant-enhanced Soil Washing to the Kuwait Soil Seriously Contaminated with the Crude Oil (원유로 심하게 오염된 쿠웨이트 토양 정화를 위한 비이온 계면활성제의 토양세척법 적용)

  • Heo, Hyojin;Lee, Minhee
    • Economic and Environmental Geology
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    • v.48 no.6
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    • pp.491-500
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    • 2015
  • Batch experiments were performed to determine the feasibility of the surfactant-enhanced soil washing process at various washing conditions for the Kuwait soil seriously contaminated with the crude oil. The soil was sampled at a dried oil pond in Kuwait and its average TPH concentration was 223,754 mg/kg, which was too high to apply the conventional remediation process. Nine commercialized non-ionic surfactants were used for the batch experiment to measure the surfactant solubility for the crude oil because it was reported that they have worked for the soil remediation. Among them, three surfactants having high crude oil solubility were used for the soil washing experiment. From the result of batch experiment, 5% TritonX-100 washing solution showed the highest TPH removal efficiency (67%) for the crude oil contaminated soil. However, because the residual TPH concentration in the washed soil was still higher than the clean-up level in Kuwait (10,000 mg/kg), the repeated soil washing was performed. After five washings with 2% surfactant solution, the cumulative TPH removal efficiency was higher than 96% and the residual TPH concentration in the soil went down below the clean-up level. To measure the desorption capacity of TritonX-100 remained in the soil after the soil washing, the silica beads and the soil were washed five times with 2% TritonX-100 surfactant solution and then they were washed again with distilled water to detach the surfactant adsorbed on beads or soil. After five washings with surfactant solution, 7.8% and 19.6% of the surfactant was adsorbed on beads and soil, respectively. When additionally washed with distilled water, most of the residual surfactant were detached from beads and only 4.3% of surfactant was remained in soil. From the results, it was investigated that the surfactant-enhanced soil washing process with TritonX-100, Tergitol S-15-7, and Tergitol S-15-9 has a great capability for the remediation of the Kuwait soil seriously contaminated by crude oil (more than 220,000 mg/kg).