• Title/Summary/Keyword: 불연속성

Search Result 217, Processing Time 0.032 seconds

Delivery of Ti Plasmid into Nicotiana sanderae Protoplasts via Liposomes (Liposome을 이용한 Ti Plasmid의 꽃담배 원형질체내 도입)

  • Lim, Myung-Ho;Jeong, Jae-Dong;Kim, In-Soo
    • Applied Biological Chemistry
    • /
    • v.37 no.5
    • /
    • pp.343-348
    • /
    • 1994
  • Ti plasmid of A. tumefaciens was labeled with $^3H-thymidine$, purified and encapsulated into phosphatidylserine (PS) and PS-cholesterol (Chol; 1 : 1 molar ratio) liposomes by lyophilization-rehydration method. PS was supplemented with 1 mole percent octadecyl rhodamine B for fluorometric measurement of PS. Liposomes entrapping $^3H-Ti plasmid$ were fused with Nicotiana sanderae protoplasts by treating with 5 mM $CaCl_2$ and 10% PEG. The fusion was evidenced by fluorescence microscopic technique. The amounts of Ti plasmid and PS associated with protoplasts were assayed by the radioactivity of $^3H-Ti plasmid$ and by the fluorescence of rhodamine B. About 7.9% of the PS liposome and 7.2% of PS-Chol liposome were fused with protoplasts. During the fusion process, about 30% of the liposomal contents of PS-Chol liposome was leaked, in contrast to about 60% leakage of its contents in PS liposome. Accounting the number of liposomes fused with protoplasts together with the encapsulation efficiency and the leakage of liposomal contents, it was calculated that ca. 1,700 Ti plasmid was transfered into one protoplast by the present method. This result may indicates that the present method transfers enough Ti plasmid into plant protoplast to elicit genetic transformation of plants.

  • PDF

Enhancement of FLDWAV Model for Its Application to the Main Reach of the Han River (한강 본류에의 적용을 위한 FLDWAV 모형의 개선)

  • Jun, Kyung-Soo;Kim, Jin-Soo;Lee, Sang-Ho
    • Journal of Korea Water Resources Association
    • /
    • v.40 no.2 s.175
    • /
    • pp.135-146
    • /
    • 2007
  • FLDWAV model was modified such that it can adequately simulate the effect of Jamsil and Singok submerged weirs in the main reach of the Han River. The enhanced model combines weir-type discharge equations for overflow at fixed weir and Manning equation for fluvial-type flow at the movable weir. Equations for weir overflow include those for submerged weir flow and free overflow. Gates of the movable weir may be open or closed for the simulation. In order to test the simulation capabilities, the enhanced model was applied for various flow conditions at submerged weirs. Backwater effect due to Jamsil and Singok submerged weirs were well simulated. Simulations were carried out for spring and neap tides extracted from artificial tide generated by combining $M_2\;and\;S_2$ tidal constituents. Simulation results cleared indicated that tidal effect extends further upstream as the flood discharge decreases. Low flow simulation capabilities of the enhanced model was tested. Discontinuities of water surface elevation due to the submerged weirs were successively simulated.

Community Structure and Floristic Composition of Cymbidium goeringii Group in Korean Islets (한반도 도서지역 춘란집단의 종조성과 군락구조)

  • Song, Hong-Seon;Park, Yong-Jin
    • FLOWER RESEARCH JOURNAL
    • /
    • v.18 no.2
    • /
    • pp.110-116
    • /
    • 2010
  • This text was analyzed and investigated the vegetation and floristic composition by ordination and classification of phytosociological method, to evaluate the species composition and community structure of Cymbidium goeringii group in Korean islets. In habitat of 33 plots, the mean altitude was 65.9m, the direction was the southeast slope, the mean slope was 7.9%. The coverage of Cymbidium goeringii was 4.5%. The appearing plants with the Cymbidium goeringii was the total 102 taxa, and it was the kind of trees 68 taxa (66.7%), herbs 34 taxa (33.3%), evergreen plants 36 taxa (35.3%) and deciduous plants 66 taxa (64.7 %) respectively. The frequency of appearing plant was the highest in the Eurya japonica (48.5%), followed by Pinus thunbergii (45.5%), Smilax china (36.4%), Carex lanceolata (33.3%), Hedera rhombea (33.3%), Machilus thunbergii (30.0%), Styrax japonicus (30.3%) and Pinus densiflora (27.3%), respectively. The vegetation of tree layer in Cymbidium goeringii group was classified into Pinus thunbergii community, Pinus densiflora community, Castanopsis sieboldii community and Quercus variabilis community. Pinus densiflora community showed a strong combination with Cymbidium goeringii group in Korean islets. Pinus thunbergii community among communities was combined with Castanopsis sieboldii community, and Pinus densiflora community and Quercus variabilis community were combined.

Foucault et la sexualité biologique (푸코와 생물학적 성 담론)

  • Hong, Eun-Young
    • Journal of Korean Philosophical Society
    • /
    • v.105
    • /
    • pp.423-460
    • /
    • 2008
  • Les positions qu'essait d'expliquer les $probl{\grave{e}}mes$ divers ${\grave{a}}$ propos de la $sexualit{\acute{e}}$, faisaient ressortir les points biologiques des actions $d^{\prime}{\hat{e}}tre$ humain par ${\acute{e}}volutionnisme$ plutot qu'explique les $probl{\grave{e}}mes$ de la nature humaine, $d{\acute{e}}sirs$ sexuelles, par les $sci{\acute{e}}t{\grave{e}}s$, les cultures et les'institutions. En face des questions des $possibilit{\acute{e}}s$ d'explications biologiques sur toutes les actions sexuelles, ils sont $plac{\acute{e}}s$ en crise. Le fait que $l^{\prime}{\hat{e}}tre$ humain est un'etre historique, n'implique pas ce qu'il est evolutionnaire. Pour cette raison, c'est difficile qu'on accepte le determisme biologique, $l^{\prime}{\acute{e}}volutionnisme$, sans conditions. En opposition avec cettes $id{\acute{e}}es$, Michel Foucault voudrait insister sur le fait de penser au sexe comme les $r{\acute{e}}sultats$ des productions sociologiques, politiques. Selon Foucault, la $sexualit{\acute{e}}$ n'est pas $donn{\acute{e}}$ de la nature que le pouvoir essaierait de mater, ou un domaine obscure que le savoir tendrait de $d{\acute{e}}voiler$, mais une sorte de dispositif historique. Donc, dans cet article, j'essaie d'examiner les processus, les differences, et les $probl{\grave{e}}mes$ entre les deux positions, puis je considere les limites philosophiques, des deux argumentations, et ses implications philosophique. Cela permet d'approcher ${\grave{a}}$ l'horizon divers du discours sexuelles de la $compr{\acute{e}}hension$.

Carbonate Breccias of the Middle Cambrian Daegi Formation, Taebaeksan Basin (태백산분지 캠브리아 중기 대기층 내의 탄산염 각력암)

  • Jang, Hwimin;Son, Mira;Ryu, In-Chang
    • Economic and Environmental Geology
    • /
    • v.52 no.5
    • /
    • pp.381-393
    • /
    • 2019
  • Carbonate breccias occur sporadically in the Middle Cambrian Daegi Formation on the southern limb of the Baegunsan Syncline, Taebaeksan Basin. These carbonate breccias have been largely interpreted either as sedimentary breccias or as tectonic, fault-related breccias. Recent study, however, indicates that the majority of these breccias are a solution-collapse breccia which is causally linked to the paleokarstification. Extensive karstification is attributed to prolonged subaerial exposure of the carbonate platform. The exposed surface is a record of interruption in sedimentation on the carbonate platform. In the stratigraphic record, such karst-related post-depositional features are recognized as meteoric diagenetic features, paleosols, and solution-collapse breccias. Solution-collapse breccias are particularly well preserved and most profound in the carbonate rocks below the major unconformities, which also are evidence of prolonged subaerial exposure. The Middle Cambrian Daegi Formation provides an example of solution-collapse breccias. The formation and preservation of the solution-collapse breccias imply that a stratigraphic discontinuity surface (unconformity) can be designated within the Daegi Formation.

Characteristics and Genesis of Terrace Soils in Yeongnam Area -IV: Soil micromorphological features (영남지역(嶺南地域)에 분포(分布)된 단구지토양(段丘地土壤)의 특성(特性)과 생성연구(生成硏究) -제(第) 4 보(報) : 토양미세형태학적(土壤微細形態學的) 특성(特性))

  • Jung, Yeun-Tae;Shin, Jae-Sung;No, Yeong-Pal
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.19 no.3
    • /
    • pp.205-210
    • /
    • 1986
  • A soil micromorphological study of clayey terrace soils in Yeongnam area was carried out to investigate the soil genetic features and to distinguish the soil parent materials. Dominant plasmic fabrics in the terrace soils were vosepic, mosepic, insepic, and masepic of sepic fabrics but the Geugrag and Upyeong in Yeongil sequence have an asepic fabrics. Embedded grain cutans in the terrace soils were characterized by having porphyroskelic related distribution. Among pedological features of the B horizons of the terrace soils, the argillic cutans of ferriargillans and glaebules of manganiferrous concretions were the dominant in mineralogical composition, and the papules are abundant in the most terrace soils indicating that the soils are generally old. The substrata of the Bancheon in Yeongcheon and Upyeong in Yeongil area were so different from the terrace deposits in plasmic fabrics that these soils were believed to be composed of bisequum profiles. Because of having the free grain cutans, it was considered that the substrata of Bancheon in Yeongcheon and Upyeong in Yeongil area might have a period of soil formation before deposition of the terrace materials.

  • PDF

The Hermeneutics of Religious Experience : Daesoon Thought in the light of Schillebeeckx's Theological Hermeneutics (종교체험의 해석적 성격: 스힐레벡스의 해석학으로 본 대순 사상)

  • Lee, Chan-Su
    • Journal of the Daesoon Academy of Sciences
    • /
    • v.17
    • /
    • pp.93-112
    • /
    • 2004
  • This article examines the process of how Daesoon thought keeps its own identity while being practiced as a religion in Korean culture through the perspective of theological hermeneutics of E. Schillebeeckx, a representative theologian in 20th century, especially on the centering of the matter of experience. Schillebeeckx says that an experience is an occurrence of perception by encountering something different from oneself. According to him, 1) the occurrence of perception is premised on a framework or form of interpretation which prescribes experience as the experience in the perceptive dimension. 2) A framework of interpretation is subject to pre-existing system or structure which already has formed a basis to the experience. It consists of various interpretative elements such as social form, general knowledges, various experiences, theoretical models in their society, etc.. 3) The experiences take place in the reflection of contemporary situation, and it is understood by a society, thus making it a social stream. This experience is expressed historically, gains historicity in the process of being handed down from person to person, from generation to generation. In this way, an experience starts to form a tradition. 4) The tradition is a historical stream which gives rise to, admits, and integrates various experiences, religious revelation, and faith etc. In this way, the tradition as a historical stream becomes a field of religious actuality such as revelation, salvation, etc. Conclusively, a religion takes place, is formed, only when it is understood anew by the people who live in that cultural tradition through their languages. The ground Daesoon thought takes place, is formed, is in this way. Daesoon thought takes place in the core of traditional Korean culture, undergoes changes when it encounters the other stream of traditional Korean culture, and the vice versa. Because of this inevitable change, ironically enough, Daesoon thought can maintain its own identity as Daesoon thought, and Korean culture its own identity as Korean culture, avoiding falling into the tertiary which is neither Daesoon thought nor Korean culture. It is in this way that any religion can be in the faithfulness to its starting point to be enriched and transformed in its interaction with the other tradition. At here is the reason Daesoon thought has to have an openness to the changing world.

  • PDF

Comparative study of laminar and turbulent models for three-dimensional simulation of dam-break flow interacting with multiarray block obstacles (다층 블록 장애물과 상호작용하는 3차원 댐붕괴흐름 모의를 위한 층류 및 난류 모델 비교 연구)

  • Chrysanti, Asrini;Song, Yangheon;Son, Sangyoung
    • Journal of Korea Water Resources Association
    • /
    • v.56 no.spc1
    • /
    • pp.1059-1069
    • /
    • 2023
  • Dam-break flow occurs when an elevated dam suddenly collapses, resulting in the catastrophic release of rapid and uncontrolled impounded water. This study compares laminar and turbulent closure models for simulating three-dimensional dam-break flows using OpenFOAM. The Reynolds-Averaged Navier-Stokes (RANS) model, specifically the k-ε model, is employed to capture turbulent dissipation. Two scenarios are evaluated based on a laboratory experiment and a modified multi-layered block obstacle scenario. Both models effectively represent dam-break flows, with the turbulent closure model reducing oscillations. However, excessive dissipation in turbulent models can underestimate water surface profiles. Improving numerical schemes and grid resolution enhances flow recreation, particularly near structures and during turbulence. Model stability is more significantly influenced by numerical schemes and grid refinement than the use of turbulence closure. The k-ε model's reliance on time-averaging processes poses challenges in representing dam-break profiles with pronounced discontinuities and unsteadiness. While simulating turbulence models requires extensive computational efforts, the performance improvement compared to laminar models is marginal. To achieve better representation, more advanced turbulence models like Large Eddy Simulation (LES) and Direct Numerical Simulation (DNS) are recommended, necessitating small spatial and time scales. This research provides insights into the applicability of different modeling approaches for simulating dam-break flows, emphasizing the importance of accurate representation near structures and during turbulence.

A Study on Applicability of Equivalent Continuum Flow Model in DFN Media (DFN 매질에 대한 등가연속체 유동모델의 적용 가능성 평가에 관한 연구)

  • Lee, Dahye;Um, Jeong-Gi
    • Tunnel and Underground Space
    • /
    • v.27 no.5
    • /
    • pp.303-311
    • /
    • 2017
  • The correlation analysis between the results obtained from DFN flow model and equivalent continuum flow model were conducted on total of 72 DFN blocks having various fracture geometry and domain size. A strong linear relation seems to exist between the two approaches under condition that normalized relative error for continuum behavior (ER) is less than 0.2, and the results from both methods are found to almost identical. To explore the field applicability of equivalent continuum flow model in DFN media, a total of 48 numerical schemes related to inflow of underground circular openings were implemented under various DFN configurations. The equivalent continuum flow model in DFN media with a constant hydraulic aperture was evaluated as valid. However, as the anisotropy increases due to variation of the hydraulic aperture, the results are likely to be overestimated compare to the DFN flow model.

Design of a spatiotemporal object model for 2D geographic objects (2차원 지리 객체를 위한 시공간 객체 모델 설계)

  • Lee, Hyeon-Ah;Nam, Kwang-Woo;Ryu, Keun-Ho
    • The KIPS Transactions:PartD
    • /
    • v.9D no.1
    • /
    • pp.43-56
    • /
    • 2002
  • Most of works have been performed on representation of spatiotemporal objects from various points of view. Most of them represent spatiotemporal objects using approaches from GIS, temporal databases, object-oriented databases or data type. Spatiotemporal objects can be classified as objects whose position and shape changes discretely over time, objects whose position changes continuously and objects whose shape changes continuously as well as position. Previous works on spatiotemporal model have focused on only one of them. In this paper, we propose a spatiotemporal model that can represent three types of objects in Euclidean plan. For this purpose, we represent both discrete and continuous moving objects by defining temporal model extended from valid time and by defining relationship between two consecutive versions of objects. The proposed spatiotemporal object model is based on open GIS specification so that it has compatibility with existing spatial data model.