• Title/Summary/Keyword: 불안정 조건

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Comparative Studies on the Enzymatic Properties of two Trypsin-like Enzymes from Menhaden, Brevoortia tyranus (혈합육어 멘헤이든의 장기조직분포Trypsin-유사효소에 관한 비교효소학적 연구)

  • PYEUN Jae-Hyeung;KIM Hyeung-Rak;GODBER J. S.
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.1
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    • pp.12-24
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    • 1990
  • Two trypsin-like enzymes, designated trypsin A and 3, purified from the intestine of menhaden by $(NH_4)_2SO_4$ fractionation, Benzamidine-Sepharose 6B affinity chromatography, DEAE-Sephacel ion exchange chromatography and Sephadex G-75 gel filtration chromatography. The two trypsins were subjected to compare the enzymatic properties of the trypsin-like enzymes from the other dark fleshed fishes. Both trypsins catalysed the hydrolysis of N$\alpha$-benzoyl-DL-arginine-p-nitroanilide and they were remarkably inhibited by several well known trypsin-inhibitors, tosyllysyl chloromethyl ketone, soybean trypsin inhibitor, be-nzamidine, leupeptin and antipain, etc. Therefore, it was ascertained that the two enzymes are serine-type trypsins. The molecular weights of these enzymes were about 25,000 and 26,200, respectively, ;Is determined by SDS-PAG electrophoresis and by Sephadex G-100 gel filtration, and the molecular weights of these two enzymes are somewhat fewer than those from the other dark fleshed fishes. Both enzymes had less basic amino acids such as arginine and Iysine, whereas they had slightly high contents of neutral amino acids, glycine, alanine and tryptophane. The enzymes showed a pH optimum of $8\~11$ at $60^{\circ}C$ against the $N\alpha$-benzoyl-DL-argi-nine-p-nitroanilide substrate and they were quite unstable above $40^{\circ}C$ and under the atidic pH region. The Km constant of the two enzymes against the $N\alpha$-benzoyl-DL-arginine-p-nitroanilide was $1.4\times10^{-4}M$ for trypsin A and $4.3\times10^{-5}M$ for trypsin B, respectively.

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Properties of Retard Type Stabilizing Agent for Reuse of Sludge Water of Ready Mixed Concrete (레미콘 회수수의 재활용을 위한 지연형 안정화제의 특성)

  • Lee Mun-Hwan;Lee Sea-Hyun;Park Young-Shin;Park Jae-Myung
    • Journal of the Korea Concrete Institute
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    • v.17 no.1 s.85
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    • pp.105-112
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    • 2005
  • Stabilizing and rousing sludge water generated from washing truck mixer and batcher plant can resolve inconsistency in quality and improve strength, therefore it is essential to review how to utilize it. This research conducted experiments and studied on solids of sludge water to find out the types of stabilizing agents available in conditions of producing ready mixed concretes in Korea. The result showed that oxy carboxylic acid retarder dedicated for stabilizing sludge water was most effective in decreasing solid. However, the setting time of cement paste was retarded due to surplus reactants, but it did not impede application of ready mixed concretes. When we left the sludge water mixed with stabilizing agent, it has been noted that initial retard effect recovered to the level using just service water in 7${\~}$8 days and that it is effective to use stabilized sludge water in 2${\~}$3 days. On the other hand, saccharic type super retarding agent was also outstanding in applicability by showing similar effect. The sludge water stabilizing agents currently marketed depend on imports, therefore it is necessary to locally manufacture usable stabilizing agents and to review its usability with multi-dimensional view.

A Method for Selecting Software Reliability Growth Models Using Partial Data (부분 데이터를 이용한 신뢰도 성장 모델 선택 방법)

  • Park, Yong Jun;Min, Bup-Ki;Kim, Hyeon Soo
    • KIPS Transactions on Software and Data Engineering
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    • v.4 no.1
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    • pp.9-18
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    • 2015
  • Software Reliability Growth Models (SRGMs) are useful for determining the software release date or additional testing efforts by using software failure data. It is not appropriate for a SRGM to apply to all software. And besides a large number of SRGMs have already been proposed to estimate software reliability measures. Therefore selection of an optimal SRGM for use in a particular case has been an important issue. The existing methods for selecting a SRGM use the entire collected failure data. However, initial failure data may not affect the future failure occurrence and, in some cases, it results in the distorted result when evaluating the future failure. In this paper, we suggest a method for selecting a SRGM based on the evaluation goodness-of-fit using partial data. Our approach uses partial data except for inordinately unstable failure data in the entire failure data. We will find a portion of data used to select a SRGM through the comparison between the entire failure data and the partial failure data excluded the initial failure data with respect to the predictive ability of future failures. To justify our approach this paper shows that the predictive ability of future failures using partial data is more accurate than using the entire failure data with the real collected failure data.

Drag Coefficient Variations of an Oscillating NACA 0012 Airfoil (진동하는 NACA 0012 에어포일에서의 항력계수 변화)

  • Kim, Dong-Ha;Chang, Jo-Won;Kim, Hak-Bong;Jeon, Chang-Soo
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.36 no.2
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    • pp.137-145
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    • 2008
  • An experimental study was performed in order to investigate the influence of Reynolds number on the drag coefficient variations of an oscillating airfoil. A NACA 0012 airfoil was sinusoidally pitched at the quarter chord point with an oscillating amplitude of ${\pm}6^{\circ}$. The free-stream velocities were 1.98, 2.83 and 4.03 m/s and the corresponding chord Reynolds numbers were $2.3{\times}10^4$, $3.3{\times}10^4$ and $4.8{\times}10^4$, respectively. The drag coefficient was calculated from the ensemble average velocity measured by an X-type hot-wire probe(X-type, 55R51) in the near-wakes region. In the case of Re=$2.3{\times}10^4$, variation of drag coefficient shows a negative damping (counter-clockwise variation), which implies an unstable state which could be excited by aerodynamic force, whereas the drag coefficient represents the positive damping (clockwise variation) as the Reynolds number increases from Re=$3.3{\times}10^4$ to $4.8{\times}10^4$. Hence, the drag coefficient variations show significant differences between Re=$2.3{\times}10^4$ and $4.8{\times}10^4$이다.

Analysis on Physical Property of Para-aramid/Nylon Hybrid filaments according to the ATY nozzle Diameter (ATY Nozzle 직경변화에 따른 Aramid/Nylon Hybrid사의 ATY 물성)

  • Park, Mi-Ra;Choi, La-Hee;Ma, Hye-Young;Park, Sung-Woo;Kim, Seung-Jin
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2012.03a
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    • pp.64-64
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    • 2012
  • 아라미드는 일반적인 유기섬유와는 다른 우수한 역학적 성질을 바탕으로 보호의류 중에서 방탄방호 및 방검보호 의류에 사용되는 고부가 소재이다. 현재까지 ATY기계에서 사의 구조와 물성에 큰 영향을 미치는 Nozzle의 구조에 대한 연구결과는 많이 발표되어왔다. 그러나 최근 들어 소방방화방 검용 보호의류에 많이 사용되는 아라미드사에 ATY 공정 중에서 Nozzle의 직경이 ATY사의 물성에 어떤 영향을 미치는가에 대한 연구는 발표된 바가 없다. 따라서 본 연구에서는 Para-aramid/Nylon hybrid사를 이용하여 ATY로 제조할 경우, 표면에 생기는 loop로 인하여 타 소재와 접착시, 접착제 담지 성능이 향상되어 접착력이 상승되는 반면 아라미드 Hybrid사의 역학물성은 ATY가 가공되기 전의 물성보다 저하되는 약점을 가지고 있다. 따라서 본 연구에서는 ATY 제조공정에서 Nozzle의 직경을 달리할 때 Aramid/Nylon Hybrid ATY사의 물성변화를 분석함으로서 방화복과 방검용 보호의류에 적합한 아라미드 ATY사를 개발하고자 한다. 본 연구에서는 ATY 제조공정 중 다른 공정조건은 동일하게 하고 Nozzle의 직경을 0.6, 0.75, 1, 1.2mm로 변경하여 4가지 시료를 준비하고 물성분석을 위하여 제조된 시료의 강신도, 초기탄성률을 각각 측정하여 인장특성을 확인하였으며, 건열수축률과 습열수축률을 측정하여 시료의 열 수축률을 측정 분석하였다. 표면의 루프 발현 정도를 보기위하여 형태 불안정성을 측정 평가하였으며 영상현미경시스템을 사용하여 표면특성을 측정 평가하여 다음과 같은 결론을 얻었다. Nozzle의 직경이 증가함에 따라 절단강도는 30% 감소하였고 초기탄성률은 3배 가까이 감소하였다. 그리고 절단신도는 2배정도 증가하는 경향을 나타내었다. 또한 Nozzle의 직경이 증가함에 따라 ATY hybrid사의 건 습열수축률이 증가하다가 직경이 1.2mm일 때 감소하는 경향을 나타내었고 직경 변화에 따라 4~6%의 열 수축률의 분포를 보였다. Para-aramid/Nylon hybrid사의 형태불안정성은 0.3~0.5%를 분포를 나타내었고 Nozzle의 직경이 0.6, 1mm일 때 상대적으로 낮은 ATY의 불안정성이 확인되었다. Nozzle의 직경이 감소할수록 loop의 엉킴이 적으며 flat하였으며 직경이 1.2mm일 때 가장 조밀하고 표면에 loop가 많이 형성된 것을 확인하였다.

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A study on the HTS-NAA/γ-spectrometry for the analysis of alpha-particle emitting impurities in silica (고순도 실리카중 알파방출 불순물 분석을 위한 HTS-NAA/γ-spectrometry 연구)

  • Lee, Kil Yong;Yoon, Yoon Yeol;Cho, Soo Young;Yang, Myung Kwon;Shim, Sang Kwon;Kim, Yongje;Chung, Yong Sam
    • Analytical Science and Technology
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    • v.18 no.1
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    • pp.5-12
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    • 2005
  • It has been established that soft error of high precision electronic circuits can be induced by alpha particles emitted from the naturally occurring radioactive impurities such as U, and Th. As the electronic circuits have recently become lower dimension and higher density, these alpha-particle emitting radioactive impurities have to be strictly controlled. The aim of this study is to develop of NAA (Neutron Activation Analysis) and gamma-spectrometry to improve the analytical sensitivity and precision of U and Th. A new NAA method has been established using the HTS (Hydrulic transfer system) irradiation facility which has been used to produce radioisotopes for industries and medicines instead of the PTS (pneumatic transfer system) irradiation facility which has been used in general NAA. When the ultratrace impurities have to be analyzed by NAA, background gamma-ray spectra induced from $^{222}Rn$ and its progenies in air is serious problem. This unstable background has been eliminated or stabilized by the use of a nitrogen purging system. Ultra trace amounts of U (0.1 ng/g) and Th (0.01 ng/g) in high purity silica used for EMC could be analyzed by the use of HTS-NAA and low background gamma-spectrometry.

A Case study on Reorganization of Specialized High School Through Workforce demand of Electric-Electronic-Communication Industry in Gyeonggi Province and SWOT Analysis (지역 기반 산업의 인력 수요와 SWOT 분석을 통한 특성화 고등학교 개편에 관한 사례연구)

  • Yi, Sang-Bong
    • 대한공업교육학회지
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    • v.36 no.2
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    • pp.65-84
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    • 2011
  • This Study was to conducted in order to suggest future direction for reorganization of Suwon ${\bigcirc}{\bigcirc}$ Women's High School as a vocational high school. The purpose of this Study was to provide suggestions for the Suwon ${\bigcirc}{\bigcirc}$ women's high school through workforce demand of electric-electronic-communication industry in Gyeonggi Province and SWOT Analysis. The findings of the study were as follows: First, the high school need to work out a educational strategy geared to workforce demand of region based electric-eElectronic-communication industry in Gyeonggi Province for students' employment. Second, the following two approaches are recommended for curriculum development to meet region based electric-eElectronic-communication industry in Gyeonggi Province for students' employment. (1) School curriculum development that is geared to a enterprise for students' employment. Demand of the enterprise is reflected when curriculum is developed under cooperation between school and enterprise. Specific knowledge, attitude and skills is emphasized to students according to demand of the enterprise. (2) school curriculum development that is geared to industry for students' employment. Demand of the electric-electronic-communication industry in Gyeonggi Province is reflected when curriculum is developed under cooperation between school and the electric-electronic-communication industry. Broader knowledge, attitude and skills is emphasized to students for the industry.

An Antifungal Subatance, 2,4-Diacetylphloroglucinol Produced from Antagonistic Bacterium Pseudo-monas fluorescens 2112 Against Phytophthora capsici (Phytophthora capsici를 길항하는 Pseudononas fluorescens 2112가 생산하는 항진균 항생물질 2,4-diacetylphloroglucinol)

  • 이은탁;김상달
    • Microbiology and Biotechnology Letters
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    • v.29 no.1
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    • pp.37-42
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    • 2001
  • An antifungal substance was purified from culture broth of Pseudomonas flulorescens 2112 that showed a broad-spectrum antagonistic activity against various phytopathogenic fungi including capsici. The substance was identified as 2,4-diacetylphloro-glucinol basd on NMR analysis. The 2,4-diacetylphloroglcinol showed antibiotic activity in broad acidic range from pH 1.0 to pH 9.0. About 83% of initial activity was remained after incubation for 30min ar $60^{\circ}C$, however, the activity was dropped up to 50% after 30 min incubation in $80^{\circ}C$. When the nucleotides of P. capsici treated with 2,4-diacetylphloroglucinol were labeled with[$^{3}$ H]-Adenin, the newly synthesized and radioactive-labeled RNA was significantly reduced than those of untreated P. capsici. indicating that the 2,4-diacetylphloroglucinol inhibits RNA synthesis.

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The Removal of Styrene using Immobilized Microorganisms in Hydrogel Beads (미생물 고정화 복합고분자담체를 이용한 Styrene 제거)

  • Song, Ji-Hyeon;Ham, Eun-Yi
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.6
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    • pp.648-653
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    • 2006
  • As an alternative for the traditional materials packed in biofilters treating gaseous VOCs, a novel packing material has been developed and tested. In the packing material(named as Hydrogel Bead, HB), pollutant-degrading microorganisms were immobilized in hydrogel consisted of alginate, polyvinyl alcohol(PVA), and powdered activated carbon. A closed-bottle study showed that the HB rapidly removed gaseous styrene without the losses of adsorption and biodegradation capacity. Biofilter column experiments using the HBs also demonstrated that greater than 95% of removal efficiencies were found at an inlet styrene loading rate of $245g/m^3/hr$, which was higher biofilter performance than other elimination capacity reported earlier. Furthermore, when the inlet styrene concentration increased stepwise, the adsorption played an important role in overall styrene removals. The absorbed styrene was found to be biodegraded in the following low inlet loading condition. Consequently, the new HB material is able to successfully minimize the drawbacks of activated carbon(necessity of regeneration) and biological processes(low removal capacity at dynamic loading conditions), and maximize the overall performance of biofilter systems treating VOCs.

Cavitation Analysis on Ship Seawater Pump Using CFD (CFD를 이용한 선박용 해수펌프의 공동현상에 대한 분석)

  • Kim, Bu-Gi;Kim, Hong-Ryeol;Yang, Chang-Jo;Kim, Jun-Ho
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.4
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    • pp.400-406
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    • 2017
  • The model used in this study was reversed to analyze the cause of excessive damage that occurred inside the rotating system and pipe system of a centrifugal-type seawater pump on a ship. For this purpose, internal flow analysis on a cooling seawater pump was performed using CFD. As a result, the shape and boundary conditions of the target pump were set by reverse engineering, and pump efficiency at a design operating point of $125m^3/h$ was calculated as 85.3 % with a head of 32.0 m. The maximum efficiency point of the target pump was estimated to be 86.2 % at $150m^3/h$, but this differed from the actual operating point. At $112.5m^3/h$, which was the lowest flow point, flow was unstable due to the characteristics of the low flow point and analysis convergence was not good. The purpose of this study was to clarify the cause of ongoing cavitation in seawater pumps and piping systems in operation. Future research will be needed to clarify causes for pipe systems in the future by performing calculations for the total piping system of an inlet and outlet, in addition to measuring the flow rate of each branch pipe.