• Resources Recycling
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• v.13 no.4
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• pp.17-22
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• 2004

This study was focused on the treatment of thickened sewage sludge. General bacteria and E. coli were disinfected over 99％ and organic compounds were decomposed after irradiation. It was suggested that this process can be an alternative for digestion Process. The moisture content in sludge was decreased over 10％ (w/w) and the coagulation of sludge particles was enhanced by irradiation at the dose of 15 kGy and addition of sea waste resource (star-fish) as a dewatering aid.

• Proceedings of the Korean Aquaculture Society Conference
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• 2004.05a
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• pp.374-375
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• 2004

• Proceedings of the Korean Aquaculture Society Conference
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• 2004.05a
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• pp.376-377
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• 2004

• Proceedings of the Korean Nutrition Society Conference
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• 2003.05a
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• pp.298.2-298.2
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• 2003

• Proceedings of the Korean Nutrition Society Conference
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• 2003.05a
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• pp.298.1-298.1
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• 2003

• Proceedings of the Materials Research Society of Korea Conference
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• 2002.11a
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• pp.176-176
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• 2002

• Development and Reproduction
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• v.10 no.1
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• pp.33-39
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• 2006

Gonadal development and reproductive cycle of the North Pacific seastar, Asterias amurensis captured from the Gosung, Gyeongsangnamdo, between November 2003 and February 2005, was investigated monthly changes of gonadosomatic index(GSI), gonadal development and oocyte size-frequency distribution. Monthly changes of GSI values showed similar trends in female and male. GSI values were reached the maximum in March($3.88{\pm}3.04$ in female, $0.87{\pm}0.57$ in male), and then gradually decreased. Based on the monthly changes of GSI, histological observation of gonadal development, reproductive cycle was divided into following successive stage: growing stage(October to January), mature stage(February to March), spent stage(March to April), degeneration and resorption stage(April to May), recovery stage(July to September). based on these result, this species seemed to have a synchronous oocyte development and one spawning season a year.

• Korean Journal of Food Science and Technology
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• v.42 no.4
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• pp.424-430
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• 2010

Immuno-modulatory activities of peptides from Asterias amurensis were investigated using a nano-encapsulation process. The molecular weights of the peptides in the range of 5-7 kDa were separated using Sephadex G-75 gel filtration. Eighty-five percent of the nano-particles were in the 300 nm range using dynamic light scattering. The cytotoxicity of the A. amurensis nano-particles against CCD-986sk human dermal fibroblast cells was 11.64% after adding 1.0 mg/mL of the samples, which was lower than that from the control (13.28% collagen). The secretion of $NO^-$ from macrophages was estimated as $40\;{\mu}M$ after adding 1.0 mg/mL of gelatin nano-particles, which was higher than the others. Prostaglandin $E_2$ production from UV-induced human skin cells decreased greatly to 860 pg/mL after adding 1.0 mg/mL of the samples. Confocal microscopy revealed that nano-particles effectively penetrated the cells within 1 hour. From these results, we consider that nano-encapsulation of the peptides from A. amurensis can improve their biological functions.

• Korean Journal of Food Science and Technology
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• v.40 no.5
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• pp.522-527
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• 2008

Low molecular peptides were isolated from Asterias amurensis via SDS-PAGE. The peptides were separated via consecutive gel filtration as five fractions (F1-F5) according to molecular weights, based on the results of MALDI-TOF MS analysis. The molecular weight of the most active peptide was estimated as 15,000 daltons. The peptide showed cytotoxicity on normal human fibroblast cells at levels as low as 20% when 1.0 mg/mL of the samples was added. The peptide also exhibited higher levels of nitric oxide production from macrophages than the lipopolysaccaharides. It was determined that prostaglendin $E_2$ production was significantly inhibited, up to 127.8% as compared to the control. The low molecular peptide inhibited hyaluronidase activity as 535.7 ${\mu}g/mL$ of $IC_{50}$. It can be concluded that the relatively low molecular weight peptide, fucoidan, from A. amurensis has excellent cosmetic and immunomodulatory activities, which can be considered as a possible resource of new cosmetic agents for skin immunomodulation.

• Journal of Life Science
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• v.28 no.8
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• pp.955-961
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• 2018

Although much research has focused on various bioactive substances in starfish, research on microorganisms isolated from starfish is lacking as compared with other natural products. In this study, we investigated bacterial communities in the gut of red starfish (Certonardoa semiregularis) in Jeju Island. In total, 103 bacterial strains were isolated using marine agar and R2A medium. The isolated strains were analyzed and identified using the 16S rRNA gene sequence. Based on an analysis of this gene sequence, the 103 isolated bacteria were classified into four major groups: Proteobacteria (93%: Alpha-proteobacteria, 24.8%; Beta-proteobacteria, 4%; Gammaproteobacteria, 65%) Bacteroidetes (4%), Actinobacteria (2%), and Firmicutes (1%). In addition, the isolates were divided into seven classes (Actinobacteria, Flavobacteria, Bacilli, Sphingobacteria, Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria), 15 orders, 19 families, and 24 genera. A phylogenetic analysis revealed two strains, Lysobacter sp. and Pedobacter sp., with similarity of 97.55% and 97.58%, respectively. As the similarity in the 16S rRNA gene sequence was 98% or less compared to previously identified bacteria, the two strains may possibly be classified as a new genus or species. We suggest that additional studies, including biochemical and morphological tests, should be performed to identify the new candidate strains.