• Title/Summary/Keyword: 분화 능력

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Beyond the Molecular Facilitator, CD82: Roles in Metastasis Suppressor, Stem Cell Niche, Muscle Regeneration, and Angiogenesis (분자 촉진제를 넘어, CD82: 전이억제자, 줄기세포 니쉬, 근육 재생 및 혈관신생에서의 역할)

  • Lee, Hyun-Chae;Han, Jung-Hwa;Hur, Jin
    • Journal of Life Science
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    • v.31 no.9
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    • pp.856-861
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    • 2021
  • CD82/KAI1, identified as a metastasis suppressor, was initially known only as a molecular facilitator, but its various functions have recently been revealed. CD82 plays an important role in the stem-progenitor cell, angiogenesis, and muscle. We would like to introduce the recently reported functions and roles of CD82 in this review. CD82 is a member of the tetraspanin family, which consists of four transmembrane domains. The interaction between CD82 and cell adhesion molecules suppresses the metastasis of cancer. CD82 regulates the cell cycle of stem-progenitor cells in the stem cell niche. In the bone marrow, CD82 is expressed on long-term repopulating hematopoietic stem cells (LT-HSCs), which show multipotent differentiation potential. The interaction between CD82 and Duffy antigen receptor for chemokines (DARC) induces quiescence in LT-HSCs. CD82 also regulates Rac1 activity, resulting in the homing and engraftment of HSCs into the bone marrow niche. Besides, CD82 maintains the differentiation potential of muscle stem cells and prevents angiogenesis by inhibiting the expression of cytokines, such as IL-6 and VEGF and adhesion molecules in endothelial cells. CD82 is a key membrane protein that distinguishes the hierarchy of stem-progenitor cells, and is also important for amplification and verification of cellular resources. Further studies on the function of CD82 in various organs and cells are expected to advance cell biology and cell therapy.

A Case Study of Family Therapy for Marriage Migrant Woman who Experienced Family Violence - Focusing on Chinese Woman Who Participated in Counseling alone - (가정폭력 피해 결혼이주여성의 가족치료 사례연구: 단독으로 상담에 참여한 중국출신 여성을 중심으로)

  • Moon, Jung Hwa
    • Korean Journal of Family Social Work
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    • no.55
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    • pp.91-128
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    • 2017
  • The purpose of this study is to develop an effective intervention strategy for marriage migrant woman in family therapy. For this purpose, we collected counseling cases of professional counselors who successfully completed counseling and attempted the qualitative analysis of treatment intervention strategies and effects. The results of the study were obtained by dividing the meaning units in the immigrant woman's statements made during the counseling process composed of a total of 6 sessions. The counselors were analyzed to have tried the following intervention strategies. They attempted the following six strategies: Helping emotional differentiation by searching for unresolved emotional problems, dealing with undifferentiation due to family projection process and love triangle, dealing with multi-generational transfer process of the original family relationship patterns and coping mechanism, shedding lihgt on ineffectiveness of inconsistent communication due to emotional oppression applying a communication model of MRI, switching client's awareness through reorganization, suggesting a way of communication that leads to real self. Such counselors' attempts resulted in positive changes and treatment effects were found to include first, cognitive insights and motivation for change, second, improved communication skills and third, anxiety reduction and self-differentiation. Due to their husbands' refusal to participate in counseling, marriage migrant women often get involved in counseling alone, so they tend to worry that the effectiveness of family therapy may be low but it was found that the proper intervention of the counselor could improve the ability of the wife to resolve conflicts, which would be a great help in solving problems such as family violence and this study is meaningful in that it provided the appropriate therapeutic intervention strategies needed.

Genetic Diversity of Rana catesbiana Captured on various sites in Korea based on mitochondrial ND1 sequence (미토콘드리아 ND1 유전자 염기서열 비교를 통한 국내 서식 황소개구리의 유전적 다양성 조사)

  • Lee Ji-Young;Shim Jae-Man;Joung Insil
    • Proceedings of the KAIS Fall Conference
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    • 2005.05a
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    • pp.297-300
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    • 2005
  • 1970년대 식용을 위한 양식을 목적으로 일본에서 수입된 황소개구리가 국내 하천과 호서생태계에 큰 피해를 주었으나, 최근 급속히 그 개체수가 줄어든 것으로 추정되므로 이번 연구에서는 국내에 서식하는 북미산 황소개구리의 유전자 분석을 통하여 개체동태군에 대한 유전적 연관을 조사하였다. 이를 위하여 전라남도 지역에서 서식하는 황소개구리를 채집하여 이미 발표된 북미산 황소개구리와 미토콘드리아 ND1/tRNA 유전자 1215bp의 염기서열을 비교, 분석하였다. 북미산과 비교하였을 때 조사한 국내 서식 개체 모두에게 ND1/tRNA 유전자 1개 위치에서 염기변화가 발견되었으나 이는 도입 개체군의 유전자인지 국내 특이변이가 진행된 것인지 확실하지 않다. 또한 조사한 개체 일부에서 유전자 염기서열의 6위치에서의 변이가 발견되었으나 국내 서식 황소개구리는 미국산 황소개구리와의 유전적 차이가 거의 없으며, Kimura-2-parameter 분석결과 국내 서식 황소개구리 개체 내에서 $98.7\%\~100\%$의 높은 유사성을 보여 종내 유전적 차이가 거의 없는 것으로 보인다. Neighbor-Joining과 Maximum Parsimony 분석 결과, cluster를 이루는 개체군의 차이를 보였으므로 개체들이 분화되어 나온 시점과 위치가 다른 것으로 확인되었지만 장흥, 영암, 고흥의 개체가 국내 도입시기의 개체군에 속하며 광주, 남평 지역의 개체군이 고흥의 한 개체로부터 분화되어 나왔음을 추정할 수 있다. 이러한 결과로부터 국내에 서식하는 황소개구리가 도입 후 지역 특이적 분화가 일어났다고 결정하기는 무리가 있으며, 이와 같이 유전적 유전도가 높은 개체들간의 교배에 따른 유전적유전적 다양성의 감소가 최근에 관찰되는 국내산 황소개구리의 급격한 감소원인들 중의 하나일 가능성을 시사한다.년도) 18,756, 2045(년도) 22,595, 시장점유율 증가로 인한 수출액 증가분 누적(억원) : 2015(년도) 3,411, 2025(년도) 8,847, 2035(년도) 14,433, 2045(년도) 18,005 또한 시나리오 비교평가를 실시하여 본 결과, 본 연구에서 정의한 순편익 누적(Cumulative Net Profit) 변수를 적용하면 현재 연구비 추세 대비 $30\%$ 까지 연구비를 증가 시키는 것이 효율적임을 알 수 있었다.성, 생산 용이성, 제품 디자인의 우수한 정도가 a=0.01 수준 하에서 유의적으로 추정되었다. 이들 변수들 중에서 품질경쟁력에 가장 큰 영향을 미치는 측정변수는 제품의 기본 성능, 수명(내구성), 신뢰성, 제품 디자인의 순서로 추정되었다. 이것은 한국 제조업이 아직 산업 디자인이 품질경쟁력에 크게 영향을 미치는 성숙단계에 이르지 못하였음을 의미한다. (2) 제품 디자인에게 영향을 끼치는 유의적인 변수는 연구개발력, 연구개발투자 수준, 혁신활동 수준(5S, TPM, 6Sigma 운동, QC 등)이며, 제품 디자인은 우선 품질경쟁력을 높여 간접적으로 고객만족과 고객 충성을 유발하는 것으로 추정되었다. 상기의 분석결과로부터, 본 연구는 다음과 같은 정책적 함의를 도출하였다. 첫째, 신상품 개발과 혁신을 위한 포괄적인 연구개발 프로젝트를 품질 경쟁력의 주요 결정요인(제품의 기본성능, 신뢰성, 수명(내구성) 및 제품 디자인)과 연계하여 추진해야 할 것이다. 둘째, 기업은 디자인 경영 마인드 제고와 디자인 전문인력 양성을, 대학은 디자인 현장 업무를 통하여 창의력 증진과 기획 및 마케팅 능력 교육을, 정부는 디자인 기술개발 및 디자인 교육지원의 강화를 통하여 각각 디자인 경쟁력$\rightarrow$품질경쟁력을 제고시켜야 할 것이

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Selection of Suitable Varieties of Carnation (Dianthus caryophyllus L.) and Optimization of Culture Conditions for Efficient Tissue Culture (효율적 조직배양체계 확립을 위한 카네이션 품종 선발 및 배양조건 설정)

  • Kang, Chan-Ho;Han, Bum-So;Han, So-Gon;Kown, Sung-Hwan;Song, Young-Ju
    • Korean Journal of Plant Resources
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    • v.24 no.2
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    • pp.121-129
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    • 2011
  • As the molecular breeding was progressed, many plant transformation techniques were attained for improving transformation accuracy and used to produce useful transgenic plants. Day by day, new varieties were developed so new transformation techniques required for these newly developed varieties. Carnation (Dianthus caryophyllus L.) is a popular and economically important ornamental plant, all over the world. Keeping this in view, we selected 18 varieties of D. caryophyllus L. commonly available in the market and did optimization of culture conditions for more efficient tissue culture and to get higher number of plants via micro-propagation. Four varieties namely Yellowdotcom, Jakarta, Belmonte, Polartessino etc. were selected for organ culture studies from single cell line. The optimum growth was recorded in the MS media supplied with sucrose 3%, NAA 1.0 mg/L and TDZ 1.0 mg/L. except Belmonte, in which, BA 1.0 mg/L was found to be the best combination, in place of TDZ, rest ingredients were same. The most efficient coagulating agent used to obtain higher number of plant from callus was phytagel 0.3%. The most effective explant for higher shoot formation was stem in which 80.2% shoot formation was recorded. It also reduced culture periods by 6 days.

Role of NFAT5 in Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells (인체 지방 유래 중간엽 줄기세포의 골분화 조절 기전에서 NFAT5의 역할)

  • Lee, Sun Young;Yang, Ji won;Jung, Jin Sup
    • Journal of Life Science
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    • v.23 no.4
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    • pp.471-478
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    • 2013
  • Human adipose tissue-derived mesenchymal stem cells (hADSCs) have therapeutic potential, including the ability to self-renew and differentiate into multiple lineages. Understanding of molecular mechanisms of stem cell differentiation is important for improving the therapeutic efficacies of stem cell transplantation. In this study, we determined the role of nuclear factor of activated T cells (NFAT5) in the osteogenic differentiation of hADSCs. The down-regulation of NFAT5 expression by the transfection of a specific siRNA significantly inhibited osteogenic differentiation of hADSCs and decreased the activity of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-${\kappa}B$) promoter without affecting their proliferation and adipogenic differentiation. The inhibition of NFAT5 expression inhibited the basal and Tumor Necrosis Factor ${\alpha}$ (TNF-${\alpha}$) induced activation of NF-${\kappa}B$, but it did not affect TNF-${\alpha}$-induced degradation of the $I{\kappa}B$ protein. These findings indicate that NFAT5 plays an important role in the osteogenic differentiation of hADSCs through the modulation of the NF-${\kappa}B$ pathway.

Reproductive Growth of Seeds and Overwintered Stump of Bulrush (Scirpus juncoides Roxb.) (올챙고랭이(Scirpus juncoides Roxb.) 종실(種實) 및 월동주기부(越冬株基部)의 번식생장(繁殖生長)에 관한 연구(硏究))

  • Huh, S.M.;Guh, J.O.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.35-44
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    • 1987
  • Differing in water conditions, the dry matter weight per plant was highest at 0 cm flooding depth, and was decreased at above 2 cm flooding depths. The shoot and spikes per pot developed best at flooding depths of 0 and 2 cm, but worst at -5cm or above 4cm flooding depths. The dry matter weight of shoots was linearly increased, but the weight of roots was sharply decreased according to high temperature after flower initation. The shoots and spikes per pot developed more effectively at 25$^{\circ}C$ than at 35 or 15$^{\circ}C$. The number of shoot and spike per pot were decreased according to higher shading. The effects of shading of 25-45% were not significantly small. The natural white and yellow spectra were the most effective to increase dry matter weight, shoots and spikes per pot. The dry matter weights of shoot and root per plant were not significantly different among at: 50 and 75% clay. The number of shoots were best and continuously increased at 75% clay, and the increments at 0 and 100% clays showed the lag period at early stage. The pattern of spikes was similar to shoots with less difference in various clay composition. The dry matter weight per plant in paddy field and upland field was basically similar. The numbers of shoot and spike were increased in response to increased fertilizer levels up to 20Kg per 10a of each component.

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On the Development of Agent-Based Online Game Characters (에이전트 기반 지능형 게임 캐릭터 구현에 관한 연구)

  • 이재호;박인준
    • Proceedings of the Korea Inteligent Information System Society Conference
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    • 2002.11a
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    • pp.379-384
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    • 2002
  • 개발적인 측면에서 온라인 게임 환경에서의 NPC(Non Playable Character)들은 환경인식능력, 이동능력, 특수 능력 및 아이템의 소유 배분 등을 원활히 하기 위한 능력들을 소유해야 하며, 게임 환경을 인식, 저장하기 위한 데이터구조와 자신만의 독특한 임무(mission)를 달성하기 위한 계획을 갖고 행위를 해야 한다. 이런 의미에서 NPC는 자신만의 고유한 규칙과 행동 패턴, 그리고 목표(Goal)와 이를 실행하기 위한 계획(plan)을 소유하는 에이전트로 인식되어야 할 것이다. 그러나, 기존 게임의 NPC 제어 구조나 구현 방법은 이러한 요구조건에 부합되지 못한 부분이 많았다. C/C++ 같은 컴퓨터 언어들을 이용한 구현은 NPC의 유연성이나, 행위에 많은 문제점이 있었다. 이들 언어의 switch 문법은 NPC의 몇몇 특정 상태를 묘사하고, 그에 대한 행위를 지정하는 방법으로 사용되었으나, 게임 환경이 복잡해지면서, 더욱더 방대한 코드를 만들어야 했고, 해석하는데 많은 어려움을 주었으며, 동일한 NPC에 다른 행동패턴을 적용시키기도 어려웠다. 또한, 대부분의 제어권을 게임 서버 폭에서 도맡아 함으로써, 서버측에 많은 과부하 요인이 되기도 하였다. 이러한 어려움을 제거하기 위해서 게임 스크립트를 사용하기도 하였지만, 그 또한 단순 반복적인 패턴에 사용되거나, 캐릭터의 속성적인 측면만을 기술 할 수 있을 뿐이었다 이러한 어려움을 해소하기 위해서는 NPC들의 작업에 필요한 지식의 계층적 분화를 해야 하고, 현재 상황과 목표 변화에 적합한 반응을 표현할 수 있는 스크립트의 개발이 필수 적이라 할 수 있다 또한 스크립트의 실행도 게임 서버 측이 아닌 클라이언트 측에서 수행됨으로써, 서버에 걸리는 많은 부하를 줄일 수 있어야 할 것이다. 본 논문에서는, 대표적인 반응형 에이전트 시스템인 UMPRS/JAM을 이용하여, 에이전트 기반의 게임 캐릭터 구현 방법론에 대해 알아본다.퓨터 부품조립을 사용해서 Template-based reasoning 예를 보인다 본 방법론은 검색노력을 줄이고, 검색에 있어 Feasibility와 Admissibility를 보장한다.매김할 수 있는 중요한 계기가 될 것이다.재무/비재무적 지표를 고려한 인공신경망기법의 예측적중률이 높은 것으로 나타났다. 즉, 로지스틱회귀 분석의 재무적 지표모형은 훈련, 시험용이 84.45%, 85.10%인 반면, 재무/비재무적 지표모형은 84.45%, 85.08%로서 거의 동일한 예측적중률을 가졌으나 인공신경망기법 분석에서는 재무적 지표모형이 92.23%, 85.10%인 반면, 재무/비재무적 지표모형에서는 91.12%, 88.06%로서 향상된 예측적중률을 나타내었다.ting LMS according to increasing the step-size parameter $\mu$ in the experimentally computed. learning curve. Also we find that convergence speed of proposed algorithm is increased by (B+1) time proportional to B which B is the number of recycled data buffer without complexity of computation. Adaptive transversal filter with proposed data recycling buffer algorithm could efficiently reject ISI of channel and increase speed of convergence in avoidance burden of computational complexity in reality when it was experimented having

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Amount of Telomeric DNA on Pig Lymphocytes by Quantitative Fluorescence in situ Hybridization (양적형광접합보인법(Q-FISH)에 의한 돼지 백혈구 세포의 텔로미어 함량 분석)

  • Sohn, Sea-Hwan;Jung, Hyun-Jin;Choi, Duk-Soon
    • Journal of Animal Science and Technology
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    • v.50 no.4
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    • pp.465-474
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    • 2008
  • The amount of telomeric DNA was quantified across different breeds(Landrace, Duroc, Yorkshire and Berksire), at different ages(90 days old and 180 days old) and among sexes(male and female) in pigs raised at the Performance Testing Station of Korea Swine Association, Jinkyo, Korea. The telomeric DNA amount was quantified by Quantitative Fluorescence In Situ Hybridization(Q-FISH) using a porcine telomeric DNA probe on interphase nuclei of lymphocytes. Analysis revealed that the amount of telomeric DNA on the pig lymphocytes was found to decrease with age. The quantity of telomeres significantly differed among breeds at 90 days of age. The colored breeds such as Berkshire and Duroc had higher amount of telomeric DNA than the Yorkshire and Landrace breed. In addition, the amount of telomeric DNA in male lymphocytes was significantly higher than that of females. In the correlation coefficients between the telomere quantity and their productive traits; average daily gain, loin percent and index value were positively correlated, whereas body length, feed efficiency and back fat thickness correlated negatively. However, the correlation coefficients were very low and not significant. Therefore, this study suggests that the amount of telomeres on lymphocytes can be considered as a physiological marker but not as a productive marker in pig.

Bioreactor Cultures of Lithospermum erythrorhizon for Shikonin Production with In Situ Extraction (동시 추출을 겸한 생물반응기에서 Lithospermum erythrorhizon 배양에 의한 shikonin 생산)

  • 김동진;장호남
    • Microbiology and Biotechnology Letters
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    • v.18 no.5
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    • pp.525-529
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    • 1990
  • Plant cell cultures of Lithospermum erythrorhizon were performed in stirred tank and packed-bed reactors with in situ extraction by n-hexadecane. The specific shikonin production and volumetric shikonin productivity of stirred tank reactor reached 1.5 mg shikoninlg cell and 400$\mu g$ shikonin/(L.day), respectively. In packed-bed reactor with calcium alginate-immobilized cells specific shikonin production and volumetric productivity reached 2.0 mg shikoninlg cell and 2857$\mu g$ shikonin/(L.day), which were 1.3 and 7.1 times higher than those of stirred tank reactor, respectively. The higher shikonin production and productivity of packed-bed reactor seemed to be due to high cell loading capacity of calcium alginate immobilized cells in packed-bed reactor and improved cell-cell contact.

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Radiotherapy Results of Malignant Astrocytoma and Glioblastoma Multiforme (악성 성상세포종과 교아세포종의 방사선 치료성적)

  • Choi, Doo-Ho;Lee, Hae-Kyung;Hong, Seong-Eon
    • Radiation Oncology Journal
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    • v.10 no.2
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    • pp.163-169
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    • 1992
  • A retrospective analysis was performed on 53 patients with brain malignant astrocytoma and glioblastoma multiforme treated with surgical resection and postoperative radiotherapy in the period between January 1980 and June 1991. There were 13 patients with malignant astrocytoma, 40 patients with glioblastoma multiforme. Survival rates were analyzed according to histologic grade, age, performance status, extent of surgical resection, tumor location, symptom duration, total radiation dose and addition of chemo­therapy after radiation therapy. 5 year actuarial survival rate for malignant astrocytoma was $29.4\%$, for glioblastoma multiforme was $2.8\%$. Histologic grade, age, performance status, total radiation dose were statistitically significant prognostic factors.

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