• Korean Journal of Plant Tissue Culture
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• v.24 no.2
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• pp.119-124
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• 1997

Plantlets were regenerated from various explants (shoot tip, leaf blade, petiole and root segments) via organogenesis and/or somatic embryogenesis from Armoracia rusticana(Lam) Gaerth., Mey, et Scherb.. Shoot regeneration rate from callus was highest on the MS mediums supplemented with 0.5 ㎎/L IAA, 5.0㎎/L BA and 10.0㎎/L spermine. A Low frequency of regeneration occurred on hormone-free MS medium. Multiple shooks were regenerated at a pH of 4.0 to 8.0 on MS medium supplemented with 1.0 ㎎/L BA and 0.1 ㎎/L NAA. Polyamines promoted shoot- and root-formation by 2 to 4 times normal, Specific proteins associated with organogenesis were identified. Somatic embryogenesis occurred directly from the leaf blade, petiole and root segments cultured on MS medium with 2.0 ㎎/L BA and 2.0 ㎎/L BA and 2.0 ㎎/L NAA. Three types of regeneration in A, rusticana were clearly established, which could be applied to the study of morphogenesis and genetics at cell, tissue and organ levels.

• Korean Journal of Remote Sensing
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• v.30 no.2
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• pp.259-274
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• 2014

On Aug 18, 2013, Sakurajima volcano in Japan erupted on a relatively large-scale. Geostationary Ocean Color Imager (GOCI) had used to detect volcanic ash in the surrounding area on the next day of this eruption. The geomagnetic variation has been analyzed using geomagnetic data from Cheongyang observatory in Korea and several geomagnetic observatories in Japan. First, we reconstruct geomagnetic data by principal component analysis and conduct semblance analysis by wavelet transform. Secondly, we minimize the error of solar effect by using wavelet based semblance filtering with Kp index. As a result of this study, we could confirm that the geomagnetic variation usually occur at the moment of Sakurajima volcano eruption. However, we cannot rule out the possibilities that it could have been impacted by other factors besides volcanic eruption in other variation's cases. This research is an exceptional study to analyze geomagnetic variation related with abroad volcanic eruption uncommonly in Korea. Moreover, we expect that it can help to develop further study of geomagnetic variation involved in earthquake and volcanic eruption.

• Reproductive and Developmental Biology
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• v.32 no.1
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• pp.1-7
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• 2008

The SCID-repopulation cells(SRCs) assay has been widely used to determine the self-renewal capacity of hematopoietic stem cells (HSCs). In this study, we tested the repopulating efficiency of porcine bone marrow derived hematopoietic stem cells using nonobese diabetic/severe combined immunodieficient (NOD/SCID) mice which was inherited immunodeficiency mire with defect of T cells, B cells, and low activity of NK cells. We transplanted porcine bone marrow hematopoietic stem/progenitor cells with intraperitoneal injection into neonate NOD/SCID mice. We confirmed efficient reconstitution activity of inoculated porcine hematopoietis cells in variety of organs of NOD/SCID mice. Interestingly, pig $CD3^+$ T lymphocytes detected with high level in liver($15.6{\pm}3.7%$), spleen($5.6{\pm}3.0%$), thymus($1.5{\pm}1.3%$), and BM($2.3{\pm}0.9%$), respectively. These data imply that microenvironment of neonate NOD/SCID mice is very efficient for proliferation and differentiation of porcine T cells, and can be useful for the study of T cells development and renogeneic organ transplantation.

• The Korean Journal of Zoology
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• v.31 no.1
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• pp.11-20
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• 1988

A comparison between two species of Hyla japonica and H. suweonesis in Korea was carried out to clarify their morphological, genetic and ecological differences, to estimate presumed divergent time between them, and to elucidate their probable process and mechanism of speciation. The results are as follows; 1.The breeding season of H. japonica was earlier than that of H. suweonensis about one and half months. 2.H.japonica was distributed all over the country, while H. suweonensis was restricted in Kyonggi plains. 3.On the extemal morphometric characters, differences were hardiy seen between two species. But on the discriminant function analysis, there was apparent differences between them. 4.Genic variation based on 24 loci showed that H. japonica populations were about two fold more variable than that of H. suweonensis populations. 5.Genetic relatedness between two species was rather remote (S =0.520, D=0.634) 6.Their presumed divergent time was estimated to be about three and a quarter mfllon years. It is presumed that H. suweonensis was originated from H. japonica during the period of late pleocene and early pleistocene. 7.Premating isolating mechanisrns (breeding season, mating call, and microhabitat segregation) may be operating to prevent gene ifow between them.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.154-160
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• 2005

To establish rapid micropropagation through organogenesis from apices-derived callus or direct adventitious shoot of three calla lily cultivars(Zantedeschia spp, cv. Sunlight, cv. Chiante, cv. Pink Persuation) were cultured on Murashige and Skoog medium supplemented with different plant growth regulators. The formation rate of callus, organogenesis and in viかo tuber production among the three cultivars were tested. Callus was obtained from cvs. Sunlight, Chiante and Pink Persuasion; the best cultivar was Sunlight. Sunlight induced $53.3\%$ callus and Chiante had the highest rate of $56.7\%$ direct shoot regeneration on medium with 2.0 mg/L BA. Regeneration frequencies ranged from 20 to $70\%$ on medium with 2.0-3.0 mg/L BA. The highest percentage of regeneration and the greatest number of shoots were obtained on medium containing 3.0 mg/L BA in three cultivars. Cytokinins induced multiple shoot formation; 1.0 mg/L of 2ip, 5.0 mg/L of BA, and 1.0 m/L of BA induced 16, 14 and 12 multiple shoots in cvs. Sunlight, Chiante and Pink Persuasion, respectivly. 1.0 mg/L of IAA enhanced root growth in cvs. Sunlight and Chiante while cv. Pink Persuasion exhibited enhanced root growth at 2.0 mg/L of IBA. NAA, however, induced no change in root growth. The addition of 90 g/L sucrose enhanced in vitro tuber formation and following tuber expansion in cv. Sunlight, while 70 g/L of sucrose was effective in cvs. Chiante and Pink Persuasion.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.85-85
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• 2019

가는잎향유[Elsholtzia angustifolia (Loes.) Kitag.]는 꽃이 원줄기와 가지 끝에 조밀하고 아름답게 달리며, 전초에 함유된 정유성분의 향이 우수하여 관상 및 약용으로 이용되고 있다. 이에 본 연구는 관상소재로 이용가능한 가는잎향유의 분화 재배기술을 확립하기 위해 2018년 7월 2일에 유묘(4월 3일~5월 31일 육묘)를 포트에 정식하여 10월 15일까지 수행하였다. 대조구는 플라스틱 화분 10호에 원예상토를 충진한 다음 200구 트레이에 셀당 4립 파종하여 생산된 유묘를 정식하여 이용하였다. 기비처리는 싱싱코트를 0, 10 및 20알로 처리하였고, 추비처리는 정식 후 4주 간격으로 hyponex (4-6-6)를 0, 1000 및 $2000mg{\cdot}L-1$로 3회 엽면시비 하였다. 차광정도 실험은 0, 55 및 75%로 광량을 달리하여 수행하였다. 왜화제는 diniconazole과 daminozide를 각 0, 1000 및 $2000mg{\cdot}L-1$로 정식 후 4주차에 처리하였다. 연구의 결과, 기비와 추비 처리는 무처리와 비교하여 생육에 큰 차이가 없었으나, 절간장은 처리량이 증가할수록 억제되었으며, 처리시 무처리에 비해 유의적으로 감소하였다. 측지수 및 엽수는 기비 처리에 따른 큰 차이가 없었으며, 추비는 측지수와 엽수가 많아지는 경향이었다. 한편, 기비와 추비 처리는 개화율을 비교적 감소시키는 결과를 보였다. 차광정도가 증가할수록 초장, 절간장, 경직경, 화서수, 화서장이 감소하는 경향이 나타났으며, 무차광과 차광처리 간 측지수와 엽수는 유의적인 차이가 나타나지 않았다. 왜화제 처리는 무처리와 비교하여 절간장을 감소시켰다. Daminozide 처리시 측지수와 엽수는 무처리에 비해 증가하였으나 유의적인 차이는 없었고, 지상부의 생체중은 엽수와 측지수에 비례하여 무거워지는 결과를 보였다. 또한, $2000mg{\cdot}L-1$ 농도 처리시 개화율이 100%에 도달하였다. Diniconazole 처리는 무처리와 비교하여 큰 차이가 없었다. 따라서, 가는잎향유를 분화재배를 하기 위해서는 기비와 추비는 처리하지 않고, 무차광 조건에서 재배하는 것이 좋다. 또한, 왜화제는 daminozide $2000mg{\cdot}L-1$로 처리하는 것이 측지수, 엽수 및 개화율이 우수한 결과를 보여 분화 재배에 적합할 것으로 판단된다.

• The Journal of the Petrological Society of Korea
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• v.4 no.2
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• pp.186-200
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• 1995

구상구조를 보이는무주 구상편마암은 전북 무주군 왕정리일대에 분포하는 정편마암인 함전기석 복운모 화강편마암내에 배태되어 있다. 구상구조는 구상편마암의 기원암인 화강암내에 포획된 이질암이 변성분화작용을 받아 생성된 것으로 사료된다. 구상편마암은 각의 발달이 없는 초생암구로 구성된 TypeI의 암구와 각의 발딜이 있는 TypeII로 구분이 가능하다. TypeII는 단각암구와 다각암구 그리고 핵의 구조에 따라 다양한 형태로 나눌수 이TEk. 구성암은 내핵, 외학, 각, 그리고 기질부로 구성된다. 핵의 장경은 보통 5cm 내지 8cm이며 구형 또는 타원형의 행태로 암구으 중심부를 이루고 있다. 핵의 화학성분은 $Al_2O_3$, total $Fe_2O_3$, MgO, $K_2O$ LREE가 풍부하고 반대로 $Na_2O$, CaO, HREE가 결정된 것이 특징이며, 핵을 주로 구성하는 변성광물은 근청석-규선석-흑운모-올리고클레이스이다. 각은 운모류의 우혹질 각과 장석류의 우백질 각으로 구분되며 수mm내지 수cm의 두께를 이루며 단일각 내지 다각구조를 이루고 있다. 이들은 핵에 비하여 $Na_2O$, CaO가 상대적으로 부화되고 있으며 기질부를 이루는 화강편마암의 조성과 유사하다. 기지루는 반상변정질로 되어 있고 장석 반상변정의 크기는 대략 2내지 3 mm의 크기로 구성되어 있으며 부수적으로 운모류와 소량의 전기석과 규선석이 존재한다. 또한 후기에 유입된 많은 유체들에 의한 후퇴변성작용의 영향으로 장석은 견운모화내지 전기석화되고, 근청석은 피나이트화 되었다.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.1-6
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• 1999

In order to obtain plantlet derived by anthers, the anthers of Lilium asiatic hybrid 'Gran Paradiso' were cultured on Murashige and Skoog's medium supplemented with various combinations of auxin and cytokinin. The most suitable pollen stage of anther culture for the callus induction was 3 days before anthesis at the early to late binucleate stage. Organogenic calli were induced on MS medium supplemented with 5.0 mg/L 2,4-D alone and the combination of 1.0 mg/L 2,4-D and 1.0 mg/L kinetin, however, the combination of NAA and BA was more effective than that of 2,4-D and kinetin on plant regeneration through organogenesis. Shoots were formed from the induced callus on the medium with 0.5 mg/L NAA and 1.0 mg/L BA after 180 days of culture. Multiple shoots with 3-4 leaves, roots, and bulblets were formed on the medium with the combination of 2.0 mg/L NAA and 2.0 mg/L BA after 250 days of culture. The chromosome from root tip of the regenerated plantlet showed the diploid (2n=2x=24). Diploid plants were transferred to the pots and all plants were flowered in two years.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.325-328
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• 2012

A new Dendranthema grandiflourm 'My Sun' was developed at Gyeonggi-do Agricultural Research & Extension Services (GARES), Korea in 2009. 'My Sun' was initially derived from the cross in 2005 between 'Omega Time Orange', a potted chrysanthemum cultivar with orange single type, and 'Tasman', a potted chrysanthemum cultivar with white single type in 2005. The cultivar has single type flowers with yellow petals. Trial and evaluation was conducted from 2006 to 2009 for the selection of this variety, including a shading culture in spring and a retarding culture in winter. The flowering time of 'My Sun' was October 13th, and year-round flowering is possible by shading or lighting treatment. The diameter of flower is 21.0 mm. Numbers of flowers per stem and petals per flower are 34.4 and 20.4, respectively. Its leaf color was green (Green Group 137A) and plant height was 13.3 cm. Days to flowering under the short day treatment is about 42 in spring, and numbers of branch per plant was 3.4 ea in the winter. This cultivar was resistance to white rust and consumer's preference of new pot-mum is high level than control.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.343-349
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• 1994

One hundred and two virulent(V) strains of Cryphonectria parasitica were isolated from the cankers of American chestnut (Castanea dentata) trees in western Massachusetts, USA. The diversity of vegetative compatibility groups (VCGs) of C. parasitica was investigated. One hundred and two strains represented 54 VCGs; 38 VCGs had only one strain each, 6 VCGs had 2 strains each, and 10 most common VCGs had 52 strains. Great diversity in VCGs may due to the increasing numbers of VCGs with time since the pathogen has been in Massachusetts for 80 years. Ten vegetative compatibility representative strains were selected from the 10 most common VCGs and converted to hypovirulent (H) strains through the pairing and hyphal anastomosis of H strains (4 strains with French dsRNA elements and 17 strains with Italian dsRNA elements). All of the 10 representative strains were converted to H strains by at least more than one of the H strains.