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Effective Reconstitution of Porcine Hematopoietic Cells in Newborn NOD/SCID Mice Xenograft  

Lee, Yong-Soo (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Lee, Hyun-Joo (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Kim, Tea-Sik (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Kim, Hye-Sun (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Kim, Yoo-Kyong (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Kim, Jae-Hwan (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Park, Jin-Ki (Animal Biotechnology Division, National Livestock Research Institute)
Chung, Hak-Jae (Animal Biotechnology Division, National Livestock Research Institute)
Chang, Won-Kyong (Animal Biotechnology Division, National Livestock Research Institute)
Kim, Dong-Ku (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Publication Information
Reproductive and Developmental Biology / v.32, no.1, 2008 , pp. 1-7 More about this Journal
Abstract
The SCID-repopulation cells(SRCs) assay has been widely used to determine the self-renewal capacity of hematopoietic stem cells (HSCs). In this study, we tested the repopulating efficiency of porcine bone marrow derived hematopoietic stem cells using nonobese diabetic/severe combined immunodieficient (NOD/SCID) mice which was inherited immunodeficiency mire with defect of T cells, B cells, and low activity of NK cells. We transplanted porcine bone marrow hematopoietic stem/progenitor cells with intraperitoneal injection into neonate NOD/SCID mice. We confirmed efficient reconstitution activity of inoculated porcine hematopoietis cells in variety of organs of NOD/SCID mice. Interestingly, pig $CD3^+$ T lymphocytes detected with high level in liver($15.6{\pm}3.7%$), spleen($5.6{\pm}3.0%$), thymus($1.5{\pm}1.3%$), and BM($2.3{\pm}0.9%$), respectively. These data imply that microenvironment of neonate NOD/SCID mice is very efficient for proliferation and differentiation of porcine T cells, and can be useful for the study of T cells development and renogeneic organ transplantation.
Keywords
Porcine; Hematopoietic stem cells; NOD/SCID mice; Engrafhnent; Differentiation;
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Times Cited By KSCI : 1  (Citation Analysis)
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